Abraham Torres - Academia.edu (original) (raw)
Papers by Abraham Torres
International Journal of Molecular Sciences
Lynch syndrome (LS) is the main hereditary colorectal cancer syndrome. There have been few report... more Lynch syndrome (LS) is the main hereditary colorectal cancer syndrome. There have been few reports regarding the clinical and molecular characteristics of LS patients in Latin America; this is particularly true in the Mexican population, where no information is available. The present study aims to describe the clinical and molecular spectrum of variants in a cohort of patients diagnosed with LS in Mexico. We present a retrospective analysis of 412 patients with suspected LS, whose main site of cancer diagnosis was the colon (58.25%), followed by the endometrium (18.93%). Next-generation sequencing analysis, with an extensive multigene panel, showed that 27.1% (112/414) had a variant in one of the genes of the mismatch repair pathway (MMR); 30.4% (126/414) had a variant in non-MMR genes such as CHEK2, APC, MUTYH, BRCA1, and BRCA2; and 42.5% (176/414) had no genetic variants. Most of the variants were found in MLH1. Pathogenic variants (PVs) in MMR genes were identified in 65.7% (96/1...
Molecular Therapy - Nucleic Acids, 2020
Archives of Virology, 2019
The Given names of the author Alma Mariana Fuentes-González was incorrectly tagged in original pu... more The Given names of the author Alma Mariana Fuentes-González was incorrectly tagged in original publication and corrected here. The original article has been corrected. Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Journal of immunology research, 2017
Systemic lupus erythematosus (SLE) is characterized by deregulated activation of T and B cells, a... more Systemic lupus erythematosus (SLE) is characterized by deregulated activation of T and B cells, autoantibody production, and consequent formation of immune complexes. Liposomes with nonbilayer phospholipid arrangements (NPA), induced by chlorpromazine, procainamide, or manganese, provoke a disease resembling human lupus when administered to mice. These mice produce anti-NPA IgM and IgG antibodies and exhibit an increased number of TLR-expressing spleen cells and a modified gene expression associated with-dependent TLR4 signaling (includingand) and complement activation. Additionally, they showed a diminished gene expression related to apoptosis and NK cell activation. We hypothesized that such gene expression may be affected by miRNAs and so miRNA expression was studied. Twelve deregulated miRNAs were found. Six of them were common to the three lupus-like models. Their validation by qRT-PCR and TaqMan probes, including miR-342-3p, revealed that miR-155-5p and miR-200a-3p expression ...
Oncology reports, 2018
Cervical cancer (CC) is one of the most common cancers diagnosed in women worldwide, and it is es... more Cervical cancer (CC) is one of the most common cancers diagnosed in women worldwide, and it is estimated that ~500,000 new patients are diagnosed with cervical cancer annually and that ~270,000 deaths occur each year. Patients with cervical cancer are treated with different radiotherapy schedules, either alone or with adjuvant chemotherapy. Unfortunately, nearly 50% of all patients with cervical cancer do not respond to standard treatment due to tumor radioresistance. In this scenario, several microRNAs (miRNAs) have been associated with the acquisition of the radioresistance phenotype. The aim of the present study was to evaluate the possible role of miR‑125a in the acquisition of radioresistance in cervical cancer. The expression of miR‑125a was assessed by means of RT‑qPCR in 30 cervical cancer samples from patients receiving standard treatment and 3 induced radioresistant cervical cancer cell lines. In addition, we employed miR‑125a mimics and inhibitors to evaluate its function...
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2017
Cervical cancer is one of the leading causes of death in women worldwide, which mainly affects de... more Cervical cancer is one of the leading causes of death in women worldwide, which mainly affects developing countries. The patients who suffer a recurrence and/or progression disease have a higher risk of developing distal metastases. Proteases comprising the degradome given its ability to promote cell growth, migration, and invasion of tissues play an important role during tumor development and progression. In this study, we used high-density microarrays and quantitative reverse transcriptase polymerase chain reaction to evaluate the degradome profile and their inhibitors in 112 samples of patients diagnosed with locally advanced cervical cancer. Clinical follow-up was done during a period of 3 years. Using a correlation analysis between the response to treatment and the development of metastasis, we established a molecular signature comprising eight degradome-related genes (FAM111B, FAM111A, CFB, PSMB8, PSMB9, CASP7, PRSS16, and CD74) with the ability to discriminate patients at ris...
International Journal of Oncology, 2016
The transcription factor PAX8, a member of the paired box-containing gene family with an importan... more The transcription factor PAX8, a member of the paired box-containing gene family with an important role in embryogenesis of the kidney, thyroid gland and nervous system, has been described as a biomarker in tumors of the thyroid, parathyroid, kidney and thymus. The PAX8 gene gives rise to four isoforms, through alternative mRNA splicing, but the splicing pattern in tumors is not yet established. Cervical cancer has a positive expression of PAX8; however, there is no available data determining which PAX8 isoform or isoforms are present in cervical cancer tissues as well as in cervical carcinoma-derived cell lines. Instead of a differential pattern of splicing isoforms, we found numerous previously unreported PAX8 aberrant transcripts ranging from 378 to 542 bases and present in both cervical carcinoma-derived cell lines and tumor samples. This is the first report of PAX8 aberrant transcript production in cervical cancer. Reported PAX8 isoforms possess differential transactivation properties; therefore, besides being a helpful marker for detection of cancer, PAX8 isoforms can plausibly exert differential regulation properties during carcinogenesis.
Gynecology and Obstetrics Research - Open Journal, 2015
Sertoli-Leydig cell tumors represent about 0.2 to 0.5% of all primary ovarian tumors. One of the ... more Sertoli-Leydig cell tumors represent about 0.2 to 0.5% of all primary ovarian tumors. One of the main features of this type of tumor is the high production of androgens, which promotes hirsutism and virilization. This case reports a 28-year-old patient with severe abdominal pain whose physical examination showed a right adnexal mass, confirmed by pelvic US, without clinical evidence of virilization, who presented elevated alpha-fetoprotein (636 ng/ mL), negative hCG-β and was negative to other tumor markers. Exploratory laparotomy was performed with right salpingo-oophorectomy. Histologically, it was identified a tumor with heterogeneous areas, retiform, tubular, microcystic, anastomosing cords and trabeculae with Leydig cells and areas of hepatoid differentiation. The tumor was positive for inhibin, cytokeratin AE1/AE3 and calretinin. One week after surgery, alpha-feto protein levels dropped to 150 ng/ ml and to 0.89 ng/ml five months later. This is a case of Sertoli-Leydig cell tumor with elevated alpha fetoprotein no evidence of virilization and the histological pattern showing focal areas of hepatoid differentiation.
Molecules, 2014
Cervical carcinoma (CC) is one of the most common cancers and a leading cause of mortality in wom... more Cervical carcinoma (CC) is one of the most common cancers and a leading cause of mortality in women worldwide. Epidemiologic and experimental data have clearly demonstrated a causal role of high-risk Human Papillomavirus (HR-HPV) types in CC initiation and progression, affecting the cellular processes by targeting and inactivating
International Journal of Molecular Sciences
Lynch syndrome (LS) is the main hereditary colorectal cancer syndrome. There have been few report... more Lynch syndrome (LS) is the main hereditary colorectal cancer syndrome. There have been few reports regarding the clinical and molecular characteristics of LS patients in Latin America; this is particularly true in the Mexican population, where no information is available. The present study aims to describe the clinical and molecular spectrum of variants in a cohort of patients diagnosed with LS in Mexico. We present a retrospective analysis of 412 patients with suspected LS, whose main site of cancer diagnosis was the colon (58.25%), followed by the endometrium (18.93%). Next-generation sequencing analysis, with an extensive multigene panel, showed that 27.1% (112/414) had a variant in one of the genes of the mismatch repair pathway (MMR); 30.4% (126/414) had a variant in non-MMR genes such as CHEK2, APC, MUTYH, BRCA1, and BRCA2; and 42.5% (176/414) had no genetic variants. Most of the variants were found in MLH1. Pathogenic variants (PVs) in MMR genes were identified in 65.7% (96/1...
Molecular Therapy - Nucleic Acids, 2020
Archives of Virology, 2019
The Given names of the author Alma Mariana Fuentes-González was incorrectly tagged in original pu... more The Given names of the author Alma Mariana Fuentes-González was incorrectly tagged in original publication and corrected here. The original article has been corrected. Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Journal of immunology research, 2017
Systemic lupus erythematosus (SLE) is characterized by deregulated activation of T and B cells, a... more Systemic lupus erythematosus (SLE) is characterized by deregulated activation of T and B cells, autoantibody production, and consequent formation of immune complexes. Liposomes with nonbilayer phospholipid arrangements (NPA), induced by chlorpromazine, procainamide, or manganese, provoke a disease resembling human lupus when administered to mice. These mice produce anti-NPA IgM and IgG antibodies and exhibit an increased number of TLR-expressing spleen cells and a modified gene expression associated with-dependent TLR4 signaling (includingand) and complement activation. Additionally, they showed a diminished gene expression related to apoptosis and NK cell activation. We hypothesized that such gene expression may be affected by miRNAs and so miRNA expression was studied. Twelve deregulated miRNAs were found. Six of them were common to the three lupus-like models. Their validation by qRT-PCR and TaqMan probes, including miR-342-3p, revealed that miR-155-5p and miR-200a-3p expression ...
Oncology reports, 2018
Cervical cancer (CC) is one of the most common cancers diagnosed in women worldwide, and it is es... more Cervical cancer (CC) is one of the most common cancers diagnosed in women worldwide, and it is estimated that ~500,000 new patients are diagnosed with cervical cancer annually and that ~270,000 deaths occur each year. Patients with cervical cancer are treated with different radiotherapy schedules, either alone or with adjuvant chemotherapy. Unfortunately, nearly 50% of all patients with cervical cancer do not respond to standard treatment due to tumor radioresistance. In this scenario, several microRNAs (miRNAs) have been associated with the acquisition of the radioresistance phenotype. The aim of the present study was to evaluate the possible role of miR‑125a in the acquisition of radioresistance in cervical cancer. The expression of miR‑125a was assessed by means of RT‑qPCR in 30 cervical cancer samples from patients receiving standard treatment and 3 induced radioresistant cervical cancer cell lines. In addition, we employed miR‑125a mimics and inhibitors to evaluate its function...
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2017
Cervical cancer is one of the leading causes of death in women worldwide, which mainly affects de... more Cervical cancer is one of the leading causes of death in women worldwide, which mainly affects developing countries. The patients who suffer a recurrence and/or progression disease have a higher risk of developing distal metastases. Proteases comprising the degradome given its ability to promote cell growth, migration, and invasion of tissues play an important role during tumor development and progression. In this study, we used high-density microarrays and quantitative reverse transcriptase polymerase chain reaction to evaluate the degradome profile and their inhibitors in 112 samples of patients diagnosed with locally advanced cervical cancer. Clinical follow-up was done during a period of 3 years. Using a correlation analysis between the response to treatment and the development of metastasis, we established a molecular signature comprising eight degradome-related genes (FAM111B, FAM111A, CFB, PSMB8, PSMB9, CASP7, PRSS16, and CD74) with the ability to discriminate patients at ris...
International Journal of Oncology, 2016
The transcription factor PAX8, a member of the paired box-containing gene family with an importan... more The transcription factor PAX8, a member of the paired box-containing gene family with an important role in embryogenesis of the kidney, thyroid gland and nervous system, has been described as a biomarker in tumors of the thyroid, parathyroid, kidney and thymus. The PAX8 gene gives rise to four isoforms, through alternative mRNA splicing, but the splicing pattern in tumors is not yet established. Cervical cancer has a positive expression of PAX8; however, there is no available data determining which PAX8 isoform or isoforms are present in cervical cancer tissues as well as in cervical carcinoma-derived cell lines. Instead of a differential pattern of splicing isoforms, we found numerous previously unreported PAX8 aberrant transcripts ranging from 378 to 542 bases and present in both cervical carcinoma-derived cell lines and tumor samples. This is the first report of PAX8 aberrant transcript production in cervical cancer. Reported PAX8 isoforms possess differential transactivation properties; therefore, besides being a helpful marker for detection of cancer, PAX8 isoforms can plausibly exert differential regulation properties during carcinogenesis.
Gynecology and Obstetrics Research - Open Journal, 2015
Sertoli-Leydig cell tumors represent about 0.2 to 0.5% of all primary ovarian tumors. One of the ... more Sertoli-Leydig cell tumors represent about 0.2 to 0.5% of all primary ovarian tumors. One of the main features of this type of tumor is the high production of androgens, which promotes hirsutism and virilization. This case reports a 28-year-old patient with severe abdominal pain whose physical examination showed a right adnexal mass, confirmed by pelvic US, without clinical evidence of virilization, who presented elevated alpha-fetoprotein (636 ng/ mL), negative hCG-β and was negative to other tumor markers. Exploratory laparotomy was performed with right salpingo-oophorectomy. Histologically, it was identified a tumor with heterogeneous areas, retiform, tubular, microcystic, anastomosing cords and trabeculae with Leydig cells and areas of hepatoid differentiation. The tumor was positive for inhibin, cytokeratin AE1/AE3 and calretinin. One week after surgery, alpha-feto protein levels dropped to 150 ng/ ml and to 0.89 ng/ml five months later. This is a case of Sertoli-Leydig cell tumor with elevated alpha fetoprotein no evidence of virilization and the histological pattern showing focal areas of hepatoid differentiation.
Molecules, 2014
Cervical carcinoma (CC) is one of the most common cancers and a leading cause of mortality in wom... more Cervical carcinoma (CC) is one of the most common cancers and a leading cause of mortality in women worldwide. Epidemiologic and experimental data have clearly demonstrated a causal role of high-risk Human Papillomavirus (HR-HPV) types in CC initiation and progression, affecting the cellular processes by targeting and inactivating