Abu Zaffar Shibly - Academia.edu (original) (raw)

Papers by Abu Zaffar Shibly

Reviews in Medical Virology, Dec 31, 2023

Nutrients

Recent evidence suggests that trimethylamine-N-oxide (TMAO), a metabolite of L-carnitine and chol... more Recent evidence suggests that trimethylamine-N-oxide (TMAO), a metabolite of L-carnitine and choline, is linked to atherosclerosis and cardiovascular diseases. As TMAO content is very high in fish, we raised the following question: why do Japanese people, who consume lots of fish, show a low risk of atherosclerosis? To address this question, we investigated the effects of TMAO and other L-carnitine-related metabolites on carotid intima–media thickness (IMT). Participants were recruited from a small island and a mountainous region. Plasma L-carnitine, γ-butyrobetaine (γBB), TMAO, trimethyllysine (TML), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) levels were measured using liquid or gas chromatography–mass spectrometry. Plasma L-carnitine concentration was higher in men than in women. TMAO and TML were significantly higher in the residents of the island than in the mountainous people. In multiple linear regression analyses in all participants, TML showed a significant ...

International Journal of Molecular Sciences

Alzheimer’s disease (AD) is a common dementia disease in the elderly. To get a better understandi... more Alzheimer’s disease (AD) is a common dementia disease in the elderly. To get a better understanding of the pathophysiology, we performed a proteomic analysis of the urine exosomes (U-exo) in AD model mice (J20). The polymer precipitation method was used to isolate U-exo from the urine of 3-month-old J20 and wild-type (WT) mice. Neuron-derived exosome (N-exo) was isolated from U-exo by immunoprecipitation. iTRAQ-based MALDI TOF MS/MS was used for proteomic analysis. The results showed that compared to WT, the levels of 61 and 92 proteins were increased in the J20 U-exo and N-exo, respectively. Gene ontology enrichment analysis demonstrated that the sphingolipid catabolic process, ceramide catabolic process, membrane lipid catabolic process, Aβ clearance, and Aβ metabolic process were highly enriched in U-exo and N-exo. Among these, Asah1 was shown to be the key protein in lipid metabolism, and clusterin, ApoE, neprilysin, and ACE were related to Aβ metabolism and clearance. Furthermo...

Suez Canal Veterinary Medicine Journal. SCVMJ, 2016

The present study was undertaken during a period from2013 to 2015 in different localities at Dami... more The present study was undertaken during a period from2013 to 2015 in different localities at Damietta Governorateto investigate the isolation prevalence of Enterococcusspp., antibacterial resistance patterensand their pathogenicity in Pekin ducks. Clinical cases from 46 duck flocks of different breedssuspected with bacterial infection (26 farms and 20backyard),were examined for the prevalence ,isolation and identification. Enterococcus faecalis. Suspected cases were confirmed after culturing on Bile esculinazid agar, biochemical testing either by conventional and bioMerieuxVitek. Prevalence were (7/26) and (4/20) in duckling farms and backyard respectively.Sensitivity testing of isolated enterococci from duck flocks to antimicrobial agents were tested using the disk diffusion method.Enterococcus faecalis showed multiple drug resistance patterns for different antibiotics ,oxytetracyclin, chloramphenicol, Erythromycin, Gentamicin, Ciprofloxacin, Neomycin, Kanamycin, Vancomycin, Nalidixic acid, and streptomycin while, susceptible to Ampicilin.Pathogencity test for isolated Enterococcus fecalis was performed .Inoculated ducks showed mainly,general clinical signs respiratory manifestation, lameness , growth retardation and pathologically airsaculitis, perihepatitis, pericarditis ,endocarditis, splenomegaly and necrosis of bone extremities (FHN) differ in severity according to the route of inoculation intravenous, intrathoracic, intranasal and orally respectively. Key word: Enterococcus, bacterial diseases, Duck diseases, arthritis in ducks, pathogenicity test in duck.

BMC Public Health, 2014

Background: Cardiovascular disease (CVD) is estimated to cause 46% of all mortalities in Kuwait. ... more Background: Cardiovascular disease (CVD) is estimated to cause 46% of all mortalities in Kuwait. To design effective primary and secondary prevention programs, an assessment of a population's prior CVD knowledge is of paramount importance. There is scarcity of data on the existing CVD knowledge among the general Kuwaiti population. Hence, this study was performed to assess the level of knowledge towards CVD types, warning symptoms of heart attack or stroke, and CVD risk factors. It also explored public views on the community pharmacists' role in CVD prevention and management. Methods: A descriptive cross-sectional survey was performed using a pretested self-administered questionnaire on a sample of 900 randomly selected Kuwaiti individuals. Descriptive and multivariate logistic regression analysis were used in data analysis. Results: The response rate was 90.7%. Respondents' knowledge about types of CVD, heart attack or stroke symptoms was low. Almost 60% of respondents did not know any type of CVD, and coronary heart disease was the commonest identified type (29.0%). Two-fifths of participants were not aware of any heart attack symptoms, and the most commonly known were chest pain (50.4%) and shortness of breath (48.0%). Approximately half of respondents did not recognize any stroke symptoms, and the most commonly recognized were 'confusion or trouble speaking' (36.4%) and 'numbness or weakness' (34.7%). Respondents' knowledge regarding CVD risk factors was moderate. The commonest factors identified by over four-fifths of participants were smoking, obesity, unhealthy diet and physical inactivity. In the multivariate logistic regression analysis, independent predictors of better level of CVD knowledge were females, age 50-59 years, high level of education, regular eating of healthy diet, and had a family history of CVD. Most of respondents only identified the role that pharmacists had to play is to help patients manage their medications, with a minimal role in other aspects of CVD prevention and management. Conclusions: There are deficiencies in CVD knowledge among Kuwaiti population, which could turn into insufficient preventative behaviours and suboptimal patient outcomes. There is an apparent need to establish more widespread and effective educational interventions, which should be sensitive to the perceptions, attitudes, and abilities of targeted individuals.

Biomedicines

Amyloid β (Aβ) peptide is deposited in the brains of sporadic Alzheimer’s disease (AD) due to imp... more Amyloid β (Aβ) peptide is deposited in the brains of sporadic Alzheimer’s disease (AD) due to impaired vessel-dependent clearance. To understand the mechanisms, we investigated time-dependent cerebrovascular changes in AD model mice. Cerebrovascular and other pathological changes were analyzed in AD model mice (J20 strain) aging from 2 to 9 months by immunostaining. At 2 months, Aβ was only intraneuronal, whereas vessels were positive from 3 months in J20 mice. Compared to wild-type (WT), vessel density was increased at 2 months but decreased at 9 months in J20 mice, claudin-5 levels were decreased, and vascular endothelial growth factor (VEGF) levels were increased in the cortex and hippocampus of J20 mice brain at all time points. Albumin extravasation was evident from 3 months in J20 brains. Collagen 4 was increased at 2 and 3 months. Aquaporin 4 was spread beyond the vessels starting from 3 months in J20, which was restricted around the vessel in wild-type mice. In conclusion, t...

Background: Plants are an integral part of life in various communities. In modern science, new dr... more Background: Plants are an integral part of life in various communities. In modern science, new drug design and for vaccination gradually depends on natural ayurvedic formulation of medicinal treatment by treating folk practitioners in the rural area. In Bangladesh, there are various rich sources of natural medicinal plants which are so much effective various ailments. Folk medicine forms the primary tier of health-care practices in Bangladesh. The objective of this study was to document the medicinal uses of plants by a folk medicinal practitioner (Kaviraj) at Kondokpur Village in Bhola district, Bangladesh. Methods: The field work was conducted during April-August 2015. Data was collected by interview with randomly selected 95 informants and Traditional Health Practitioner (THP) living in the study area. Results: The Kaviraj (folk practitioner) used 10 plants distributed into 10 families in his formulations. The formulations were used to treat diverse diseases like gastrointestinal disorders, skin disorders, pain, fever, burns, infertility, diabetes, jaundice, diarrhea, stomach pain, inflammation, dysentery, tuberculosis, asthma and cuts and wounds. Conclusion: The present study revealed that some of the well-known medicinal plants are used an effective ethnobotanical practice in our study area. Moreover herbal plants used by the Kaviraj of Bhola district needs to be scientifically studied towards discovery of useful drugs.

Journal of clinical trials, 2021

Background: Pueraria Decoction (PD) is a Japanese herbal medicine of Kampo tradition, which is us... more Background: Pueraria Decoction (PD) is a Japanese herbal medicine of Kampo tradition, which is used for acute febrile diseases, inflammatory disease and allergic rhinitis. Moreover, PD is reported to have beneficial effects on autonomic disturbance in patients. Objective: The objective of the study is to investigate the effects of PD on autonomic nervous system of healthy adult subjects using spectral analysis of heart rate and blood pressure variability during Head-Up Tilt Test (HUTT). Here, we investigated the effects of PD on autonomic nervous system of healthy adults using spectral analysis of heart rate and blood pressure variability during Head-Up Tilt Test (HUTT). Methods: Twenty healthy subjects were divided into young and middle-aged groups, and examined twice with HUTT, before and 5 minutes after taking 5 g of PD. Spectral analysis of RR interval and Systolic Blood Pressure (SBP) variability was then used to measure the changes in autonomic functions. Results: As for all s...

Background: Folk ayurvedic pratitioners are the primary health care providers in rural population... more Background: Folk ayurvedic pratitioners are the primary health care providers in rural population in Bangladesh.in Bengali term; these medicinal practitioners are known as “Kavirajes”. Ayurvedic studies are an important feature of traditional medicinal practices in Bangladesh. The major characteristic that separates the folk medicinal practitioners from other systems of existing medicinal practices is their almost exclusive use of simple preparations of medicinal plants for treatment of various ailments. The objective of this study was to make a comparative analysis of reported ethnomedicinal uses of plants used by a folk medicinal practitioner (Kaviraj) of paramtala village of Muradnagar Upazila in comilla district, Bangladesh. Methods: Interviews of the Kaviraj were carried out with the help of a semistructured questionnaire and the guided field-walk method. Results: The Kaviraj used 12 plants distributed into 10 families in his formulations. Available ethnomedicinal and scientifi...

Diabetes mellitus (DM) is a common and metabolic disorder throughout the world. In recent years, ... more Diabetes mellitus (DM) is a common and metabolic disorder throughout the world. In recent years, there have been various types of research and survey works are studied. Bangladesh is the land of beauty whereas the natural plants have exclusive medicinal ayurvedic activity against Insulin Dependent Diabetic Mellitus (IDDM) and Non-Insulin Dependent Diabetic Mellitus (NIDDM). Among many medications and other alternative medicines, several herbs have been known to cure and control diabetes; additionally they have no side effects. In the last few years, there has been an exponential growth in the field of herbal medicine and gaining popularity both in developing and developed countries because of their natural origin and less side effects. In this review work, we just figure out some potential herbal plants (25) antidiabetic activity in Bangladesh. A comprehensive review of the present paper is an attempt to list of the plants with anti-diabetic and related beneficial effects originatin...

Journal of Biomolecular Structure and Dynamics, 2020

Background: Chikungunya virus (CHIKV) instigating Chikungunya fever is a global infective menace ... more Background: Chikungunya virus (CHIKV) instigating Chikungunya fever is a global infective menace resulting in high fever, weakened joint-muscle pain, and brain inflammation. Inaccessibility and unavailability of effective drugs have led us to an uncertain arena when it comes to providing proper medical treatment to the affected people. Method: In this study, authentic encroachment has been made concerning the peptide-based epitope vaccine designing against CHIKV. A Proteome-wide search was performed to locate a conserved portion among the accessible viral outer membrane proteins which showcase a remarkable immune response using specific immunoinformatics and docking simulation tools. Results: Primarily, the most probable immunogenic envelope glycoproteins E1 and E2 were identified from the UniProt database depending on their antigenicity scores. Subsequently, we selected two distinctive sequences "SEDVYANTQLVLQRP" and "IMLLYPDHPTLLSYR" in both E1 and E2 glycoproteins respectively. These two sequences identified as the most potent T and B cell epitope-based peptides as they interacted with 6 and 7 HLA-I and 5 HLA-II molecules with an extremely low IC50 score that was verified by molecular docking. Moreover, the sequences possess no allergenicity and are certainly located outside the transmembrane region. In addition, the sequences exhibited 88.46% and 100.00% Conservancy, covering high population coverage of 89.49% to 94.74% and 60.51% to 88.87% respectively in endemic countries. Conclusion: The identified peptide SEDVYANTQLVLQRP and IMLLYPDHPTLLSYR can be utilized next for the development of peptide-based epitope vaccine contrary to CHIKV, So further documentations and experimentations like Antigen testing, Antigen production, Clinical trials are needed to prove the validity of it.

3 Biotech, 2018

Lassa virus (LASV) is responsible for an acute viral hemorrhagic fever known as Lassa fever. Sequ... more Lassa virus (LASV) is responsible for an acute viral hemorrhagic fever known as Lassa fever. Sequence analyses of LASV proteome identified the most immunogenic protein that led to predict both T-cell and B-cell epitopes and further target and binding site depiction could allow novel drug findings for drug discovery field against this virus. To induce both humoral and cell-mediated immunity peptide sequence SSNLYKGVY, conserved region 41-49 amino acids were found as the most potential B-cell and T-cell epitopes, respectively. The peptide sequence might intermingle with 17 HLA-I and 16 HLA-II molecules, also cover 49.15-96.82% population coverage within the common people of different countries where Lassa virus is endemic. To ensure the binding affinity to both HLA-I and HLA-II molecules were employed in docking simulation with suggested epitope sequence. Further the predicted 3D structure of the most immunogenic protein was analyzed to reveal out the binding site for the drug design ...

African Journal of Traditional, Complementary and Alternative Medicines, 2016

Background: The aim of the study was to investigate the hypoglycemic effects of Phloridzin. Metho... more Background: The aim of the study was to investigate the hypoglycemic effects of Phloridzin. Methods and Materials: High fat diet induced diabetic KKAy mice were administered with phloridzin at an oral dose (60 mg/kg/day, ig.) for 10 weeks. A range of parameters, including blood glucose and lipid, serum insulin, glucose tolerance, were tested to evaluate its anti-hyperglycemic effects. Results: Phloridzin decreased water-intake, body weight, FBG, FINS, HOME-IR, Serum leptin, and CRP levels, increased serum adiponectin level in diabetic mice. Phloridzin also improved the oral glucose tolerance test (OGTT) to a certain degree. In addition, phloridzin decreased liver index, and epididymal, perirenal white adipose tissue indexs, increased pancreas index in diabetic mice. At last, phloridzin increased hepatic GK activity and hepatic glycogen level, decreased hepatic PEPCK, G-6-Pase activities in diabetic mice. Conclusions: Phloridzin possessed anti-hyperglycemic activities.

Advances in Breast Cancer Research, 2017

The study was conducted to ensure knowledge, attitude, awareness about breast and cervical cancer... more The study was conducted to ensure knowledge, attitude, awareness about breast and cervical cancer among the female students of Mawlana Bhashani Science and Technology University. This study was carried out from July to September, 2015 among the participants of university female halls, different female hostels and different faculty of the university by using a validated questionnaire which was developed for this study. A total 250 female students, at the age of (18-26) years were participated. Collected information was analyzed using SPSS, Graph-pad Prism and MS Excel. The results showed that about 87.6% participants were undergraduate. Most of them come from village (45.6%) and city (36.0%). About 90% were Muslims and the socioeconomic level of most of the participants was middle (90.4%). In case of food intake patterns, it was observed that 35.6% participants eat chips, soft drinks, popcorn everyday; 40.4% eat meat regularly; 24% eat sugar everyday; 27.6% eat fruits and vegetables every day. Among them, 55.6% girls maintained daily 1 hour physical activity; 37.2% did exercise rarely; 34.8% participants rarely do strenuous exercise. About 84.4% respondents have not any family history of cancer; 6% participants have sister or mother having breast tumor and 7.2% have at least more than one close relative who have cancer. Among the participants, only 0.8% drank alcohol; 1.2% have addiction of smoking cigarette; 15.6% girls wear tight bra; 3.6% have benign breast disease and 2.8% participants have attended in breast or cervical cancer screening programs.

Biochemistry & Physiology: Open Access, 2016

Asian Pacific Journal of Tropical Biomedicine, 2016

Leonurus sibiricus is a herbaceous plant found in many countries in Asia and America. This plant ... more Leonurus sibiricus is a herbaceous plant found in many countries in Asia and America. This plant is widely practiced as a remedy for the treatment of diabetes, menstrual irregularities, and bronchitis. The approval of therapeutic implications of any drugs depends on the well characterized mode of actions of the compounds. The bioactive compounds like diterpenes, triterpenes, flavonoids and phenolic acids in Leonurus sibiricus show analgesic, anti-inflammatory, anti-oxidant, anti-atherogenic and antihemorrhagic, anti-diabetic, anti-bacterial and allelopathic potency. Interestingly, the expression level of some genes is altered by the crude extract treatments, which are effective against cancers, diabetes and cardiovascular diseases where the molecular mechanisms are yet to be explored. Intriguingly, the extracts significantly induce nitric oxide production by endothelial nitric oxide synthase, a signaling molecule of vasodilation in combination with interferon-g indicating positive effect on atherosclerosis. Further investigations are required to unlock the effects of bioactive compounds found in extracts at clinical settings.

Bioinformation, 2016

A metabolic disorder (MD) occurs when the metabolic process is disturbed. This process is carried... more A metabolic disorder (MD) occurs when the metabolic process is disturbed. This process is carried out by thousands of enzymes participating in numerous inter-dependent metabolic pathways. Critical biochemical reactions that involve the processing and transportation of carbohydrates, proteins and lipids are affected in metabolic diseases. Therefore, it is of interest to identify the common pathways of metabolic disorders by building protein-protein interactions (PPI) for network analysis. The molecular network linkages between MD and age related diseases (ARD) are intriguing. Hence, we created networks of protein-protein interactions that are related with MD and ARD using relevant known data in the public domain. The network analysis identified known MD associated proteins and predicted genes and or its products of ARD in common pathways. The genes in the common pathways were isolated from the network and further analyzed for their co-localization and shared domains. Thus, a model hy...

Biochemistry & Physiology: Open Access, 2015

The form of programmed cell death known as Apoptosis has become an intense focus of investigation... more The form of programmed cell death known as Apoptosis has become an intense focus of investigation in various fields including carcinogenesis and cancer therapy. It is a sequentially regulated suicidal programme where cells regulate certain enzymes including caspase 9 activation and cytochrome C release with apoptosis inducing factor (AIF). The Bcl-2 proteins also represent a promising target for modulating tumor cell sensitivity to Apoptosis. Disturbance of this regulatory pathway may lead to various diseases like autoimmune diseases, neurodegenerative diseases and cancers. Therefore, understanding the mechanisms for apoptosis signaling pathway will give us huge knowledge to enlighten the pathogenesis of various diseases including cancer, and will open new horizons to therapeutic approaches in drug designing.

The effect of many calcium salts and CaC2 is diverge on the ripening of the mango fruits in Bangl... more The effect of many calcium salts and CaC2 is diverge on the ripening of the mango fruits in Bangladesh, which is frequently found. Green and mature fresh mango fruits (mangefera indica, four types of subgroup was taken, lokhna-mangefera indica, hemsagor-mangefera indica, gopalvogh – mangefera indica, and langra-mangefera indica) were harvested and immersed for l0 minutes in different calcium salt concentration. CaC2 and three calcium salts were taken in four concentrations – 2.5, 5.0, 7.5 and 10 %. The salts were –calcium chloride (CaCl2), calcium sulphate (CaSO4) and calcium ammonium nitrate Ca[NH4NO3]2. For the accuracy of the result, a control for every concentration was also included in which fruits were dipped in fresh water for 10 minutes. The mango fruits were ripened at ambient temperature (27°±4°c) in the paper carton–boxes lined and covered with newspapers. CaC2 acts as a fast ripening agent. but Calcium salts delayed the fruits ripening about 3 days as compared to the con...