Adelola Oseni - Academia.edu (original) (raw)

Papers by Adelola Oseni

Research paper thumbnail of Contributor contact details

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Research paper thumbnail of Assessing the chondrogenic potential of a novel biometic nanocomposite polymer for tissue engineering a nose: The chondrocyte response to nanoscale

Assessing the chondrogenic potential of a novel biometic nanocomposite polymer for tissue engineering a nose: The chondrocyte response to nanoscale

Research paper thumbnail of Dynamic control of head mesoderm patterning

Development, 2011

The embryonic head mesoderm gives rise to cranial muscle and contributes to the skull and heart. ... more The embryonic head mesoderm gives rise to cranial muscle and contributes to the skull and heart. Prior to differentiation, the tissue is regionalised by the means of molecular markers. We show that this pattern is established in three discrete phases, all depending on extrinsic cues. Assaying for direct and first-wave indirect responses, we found that the process is controlled by dynamic combinatorial as well as antagonistic action of retinoic acid (RA), Bmp and Fgf signalling. In phase 1, the initial anteroposterior (a-p) subdivision of the head mesoderm is laid down in response to falling RA levels and activation of Fgf signalling. In phase 2, Bmp and Fgf signalling reinforce the a-p boundary and refine anterior marker gene expression. In phase 3, spreading Fgf signalling drives the a-p expansion of MyoR and Tbx1 expression along the pharynx, with RA limiting the expansion of MyoR. This establishes the mature head mesoderm pattern with markers distinguishing between the prospectiv...

Research paper thumbnail of The application of POSS nanostructures in cartilage tissue engineering: the chondrocyte response to nanoscale geometry

Journal of Tissue Engineering and Regenerative Medicine, 2013

Despite extensive research into cartilage tissue engineering (CTE), there is still no scaffold id... more Despite extensive research into cartilage tissue engineering (CTE), there is still no scaffold ideal for clinical applications. Various synthetic and natural polymers have been investigated in vitro and in vivo, but none have reached widespread clinical use. The authors investigate the potential of POSS-PCU, a synthetic nanocomposite polymer, for use in CTE. POSS-PCU is modified with silsesquioxane nanostructures that improve its biological and physical properties. The ability of POSS-PCU to support the growth of ovine nasoseptal chondrocytes was evaluated against a polymer widely used in CTE, polycaprolactone (PCL). Scaffolds with varied concentrations of the POSS molecule were also synthesized to investigate their effect on chondrocyte growth. Chondrocytes were seeded onto scaffold disks (PCU negative control; POSS-PCU 2%, 4%, 6%, 8%; PCL). Cytocompatibilty was evaluated using cell viability, total DNA, collagen and GAG assays. Chondrocytes cultured on POSS-PCU (2% POSS) scaffolds had significantly higher viability than PCL scaffolds (p < 0.001). Total DNA, collagen and sGAG protein were also greater on POSS-PCU scaffolds compared with PCL (p > 0.05). POSS-PCU (6% and 8% POSS) had improved viability and proliferation over an 18 day culture period compared with 2% and 4% POSS-PCU (p < 0.0001). Increasing the percentage of POSS in the scaffolds increased the size of the pores found in the scaffolds (p < 0.05). POSS-PCU has excellent potential for use in CTE. It supports the growth of chondrocytes in vitro and the POSS modification significantly enhances the growth and proliferation of nasoseptal chondrocytes compared with traditional scaffolds such as PCL.

Research paper thumbnail of Cartilage Tissue Engineering: the Application of Nanomaterials and Stem Cell Technology

Tissue Engineering for Tissue and Organ Regeneration, 2011

Research paper thumbnail of Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration

Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration

Methods in Molecular Biology, 2013

Autologous hydrogel manufacture is an exciting technique within the field of regenerative medicin... more Autologous hydrogel manufacture is an exciting technique within the field of regenerative medicine. Fibrin is a protein with many biocompatible and regenerative features. The ability to generate fibrin scaffolds with the necessary matrix topography for cell integration, from a patient&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s autologous tissue, could improve the translation of many tissue engineering efforts from bench to clinical application. Here we describe the rapid extraction and production of fibrin hydrogels for development of organs, using a simple low-cost series of centrifugations and ethanol precipitation, which produces hydrogels of a more predictable amount and morphology.

Research paper thumbnail of Advancing nasal reconstructive surgery: the application of tissue engineering technology

Journal of Tissue Engineering and Regenerative Medicine, 2012

Cartilage tissue engineering is a rapidly progressing area of regenerative medicine with advances... more Cartilage tissue engineering is a rapidly progressing area of regenerative medicine with advances in cell biology and scaffold engineering constantly being investigated. Many groups are now capable of making neocartilage constructs with some level of morphological, biochemical, and histological likeness to native human cartilage tissues. The application of this useful technology in articular cartilage repair is well described in the literature; however, few studies have evaluated its application in head and neck reconstruction. Although there are many studies on auricular cartilage tissue engineering, there are few studies regarding cartilage tissue engineering for complex nasal reconstruction. This study therefore highlighted the challenges involved with nasal reconstruction, with special focus on nasal cartilage tissue, and examined how advancements made in cartilage tissue engineering research could be applied to improve the clinical outcomes of total nasal reconstructive surgery. Abbreviations used: AD-MSC, adipose derived mesenchymal derived stem cells; BMSC, bone marrow stromal cells; BM-MSC, bone marrow derived mesenchymal stem cells; Col, collagen; ELP, elastic-like polypeptide; ESC, embryonic stem cells; sGAG, sulphated glycosaminoglycans; PCL, poly (caprolactone); SMSC, synovium derived mesenchymal stem cell. 762 A. Oseni et al.

Research paper thumbnail of Optimization of chondrocyte isolation and characterization for large-scale cartilage tissue engineering

Journal of Surgical Research, 2013

Harvest Nasoseptal cartilage Dispase Hyaluronidase Pronase Collagenase II a b s t r a c t Backgro... more Harvest Nasoseptal cartilage Dispase Hyaluronidase Pronase Collagenase II a b s t r a c t Background: Advancements in cartilage tissue engineering have the potential to ameliorate facial and joint reconstructive surgery as we know it. The translation of in vitro models of cartilage regeneration into clinical scenarios is the next phase of cartilage tissue engineering research. To engineer larger, more robust, and clinical relevant constructs, a great number of viable chondrocytic cells are needed. However, there is a paucity of literature concerning the most favorable method of chondrocyte isolation. Isolation methods are inconsistent, resulting in small yields and poor cell quality, and thus unreliable neocartilage formation. This study aimed to optimize the chondrocyte isolation protocol to give a maximum yield with optimal cell viability for the engineering of large cartilaginous constructs such as the human nose and ear. Methods: We employed several enzymes (pronase, dispase, hyaluronidase, and collagenase), enzyme concentrations, and digest lengths to digest freshly harvested ovine nasoseptal cartilage. We used automated trypan blue live/dead staining, immunofluorescent labeling of CD44, collagenase II, collagenase I, and Aggrecan, and alamarBlue to assess cell yield and viability. Results: Incubation length in enzymatic solutions had the greatest effect on cell viability, whereas concentrations of enzymes had a lesser effect. Isolated cells maintained their expression of chondrocyte-specific cell surface markers.

Research paper thumbnail of Nasal Reconstruction Using Tissue Engineered Constructs

Nasal Reconstruction Using Tissue Engineered Constructs

Annals of Plastic Surgery, 2013

Currently, the gold standard for reconstruction after rhinectomy or severe trauma to the nose, in... more Currently, the gold standard for reconstruction after rhinectomy or severe trauma to the nose, includes transposition of autologous mucosal flaps plus autologous cartilage grating and coverage using a skin flap. Difficulties with this approach arise where; cartilage and mucosa harvested from autologous donor sites is insufficient to achieve a passable aesthetic and functional reconstruction. Skin flaps are often bulky, poor color matches with hair follicles that reduce the aesthetic quality of the reconstruction. We suggest that tissue engineering could be a source of functional replacement tissues for nasal reconstructive surgery. However, the advancement of such an approach is dependent on the dissemination of scientific information into the clinical community, regarding the engineering of tissues such as mucosa, skin, and cartilage. This paper therefore reviews how the tissue engineering strategies available for producing clinically viable tissues could help resolve issues around reconstructing the human nose.

Research paper thumbnail of The application of POSS nanostructures in cartilage tissue engineering: the chondrocyte response to nanoscale geometry

The application of POSS nanostructures in cartilage tissue engineering: the chondrocyte response to nanoscale geometry

Journal of Tissue Engineering and Regenerative Medicine, 2013

Despite extensive research into cartilage tissue engineering (CTE), there is still no scaffold id... more Despite extensive research into cartilage tissue engineering (CTE), there is still no scaffold ideal for clinical applications. Various synthetic and natural polymers have been investigated in vitro and in vivo, but none have reached widespread clinical use. The authors investigate the potential of POSS-PCU, a synthetic nanocomposite polymer, for use in CTE. POSS-PCU is modified with silsesquioxane nanostructures that improve its biological and physical properties. The ability of POSS-PCU to support the growth of ovine nasoseptal chondrocytes was evaluated against a polymer widely used in CTE, polycaprolactone (PCL). Scaffolds with varied concentrations of the POSS molecule were also synthesized to investigate their effect on chondrocyte growth. Chondrocytes were seeded onto scaffold disks (PCU negative control; POSS-PCU 2%, 4%, 6%, 8%; PCL). Cytocompatibilty was evaluated using cell viability, total DNA, collagen and GAG assays. Chondrocytes cultured on POSS-PCU (2% POSS) scaffolds had significantly higher viability than PCL scaffolds (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). Total DNA, collagen and sGAG protein were also greater on POSS-PCU scaffolds compared with PCL (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05). POSS-PCU (6% and 8% POSS) had improved viability and proliferation over an 18 day culture period compared with 2% and 4% POSS-PCU (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). Increasing the percentage of POSS in the scaffolds increased the size of the pores found in the scaffolds (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). POSS-PCU has excellent potential for use in CTE. It supports the growth of chondrocytes in vitro and the POSS modification significantly enhances the growth and proliferation of nasoseptal chondrocytes compared with traditional scaffolds such as PCL. Copyright © 2013 John Wiley &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Sons, Ltd.

Research paper thumbnail of Contributor contact details

Contributor contact details

Research paper thumbnail of Assessing the chondrogenic potential of a novel biometic nanocomposite polymer for tissue engineering a nose: The chondrocyte response to nanoscale

Assessing the chondrogenic potential of a novel biometic nanocomposite polymer for tissue engineering a nose: The chondrocyte response to nanoscale

Research paper thumbnail of Dynamic control of head mesoderm patterning

Development, 2011

The embryonic head mesoderm gives rise to cranial muscle and contributes to the skull and heart. ... more The embryonic head mesoderm gives rise to cranial muscle and contributes to the skull and heart. Prior to differentiation, the tissue is regionalised by the means of molecular markers. We show that this pattern is established in three discrete phases, all depending on extrinsic cues. Assaying for direct and first-wave indirect responses, we found that the process is controlled by dynamic combinatorial as well as antagonistic action of retinoic acid (RA), Bmp and Fgf signalling. In phase 1, the initial anteroposterior (a-p) subdivision of the head mesoderm is laid down in response to falling RA levels and activation of Fgf signalling. In phase 2, Bmp and Fgf signalling reinforce the a-p boundary and refine anterior marker gene expression. In phase 3, spreading Fgf signalling drives the a-p expansion of MyoR and Tbx1 expression along the pharynx, with RA limiting the expansion of MyoR. This establishes the mature head mesoderm pattern with markers distinguishing between the prospectiv...

Research paper thumbnail of The application of POSS nanostructures in cartilage tissue engineering: the chondrocyte response to nanoscale geometry

Journal of Tissue Engineering and Regenerative Medicine, 2013

Despite extensive research into cartilage tissue engineering (CTE), there is still no scaffold id... more Despite extensive research into cartilage tissue engineering (CTE), there is still no scaffold ideal for clinical applications. Various synthetic and natural polymers have been investigated in vitro and in vivo, but none have reached widespread clinical use. The authors investigate the potential of POSS-PCU, a synthetic nanocomposite polymer, for use in CTE. POSS-PCU is modified with silsesquioxane nanostructures that improve its biological and physical properties. The ability of POSS-PCU to support the growth of ovine nasoseptal chondrocytes was evaluated against a polymer widely used in CTE, polycaprolactone (PCL). Scaffolds with varied concentrations of the POSS molecule were also synthesized to investigate their effect on chondrocyte growth. Chondrocytes were seeded onto scaffold disks (PCU negative control; POSS-PCU 2%, 4%, 6%, 8%; PCL). Cytocompatibilty was evaluated using cell viability, total DNA, collagen and GAG assays. Chondrocytes cultured on POSS-PCU (2% POSS) scaffolds had significantly higher viability than PCL scaffolds (p < 0.001). Total DNA, collagen and sGAG protein were also greater on POSS-PCU scaffolds compared with PCL (p > 0.05). POSS-PCU (6% and 8% POSS) had improved viability and proliferation over an 18 day culture period compared with 2% and 4% POSS-PCU (p < 0.0001). Increasing the percentage of POSS in the scaffolds increased the size of the pores found in the scaffolds (p < 0.05). POSS-PCU has excellent potential for use in CTE. It supports the growth of chondrocytes in vitro and the POSS modification significantly enhances the growth and proliferation of nasoseptal chondrocytes compared with traditional scaffolds such as PCL.

Research paper thumbnail of Cartilage Tissue Engineering: the Application of Nanomaterials and Stem Cell Technology

Tissue Engineering for Tissue and Organ Regeneration, 2011

Research paper thumbnail of Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration

Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration

Methods in Molecular Biology, 2013

Autologous hydrogel manufacture is an exciting technique within the field of regenerative medicin... more Autologous hydrogel manufacture is an exciting technique within the field of regenerative medicine. Fibrin is a protein with many biocompatible and regenerative features. The ability to generate fibrin scaffolds with the necessary matrix topography for cell integration, from a patient&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s autologous tissue, could improve the translation of many tissue engineering efforts from bench to clinical application. Here we describe the rapid extraction and production of fibrin hydrogels for development of organs, using a simple low-cost series of centrifugations and ethanol precipitation, which produces hydrogels of a more predictable amount and morphology.

Research paper thumbnail of Advancing nasal reconstructive surgery: the application of tissue engineering technology

Journal of Tissue Engineering and Regenerative Medicine, 2012

Cartilage tissue engineering is a rapidly progressing area of regenerative medicine with advances... more Cartilage tissue engineering is a rapidly progressing area of regenerative medicine with advances in cell biology and scaffold engineering constantly being investigated. Many groups are now capable of making neocartilage constructs with some level of morphological, biochemical, and histological likeness to native human cartilage tissues. The application of this useful technology in articular cartilage repair is well described in the literature; however, few studies have evaluated its application in head and neck reconstruction. Although there are many studies on auricular cartilage tissue engineering, there are few studies regarding cartilage tissue engineering for complex nasal reconstruction. This study therefore highlighted the challenges involved with nasal reconstruction, with special focus on nasal cartilage tissue, and examined how advancements made in cartilage tissue engineering research could be applied to improve the clinical outcomes of total nasal reconstructive surgery. Abbreviations used: AD-MSC, adipose derived mesenchymal derived stem cells; BMSC, bone marrow stromal cells; BM-MSC, bone marrow derived mesenchymal stem cells; Col, collagen; ELP, elastic-like polypeptide; ESC, embryonic stem cells; sGAG, sulphated glycosaminoglycans; PCL, poly (caprolactone); SMSC, synovium derived mesenchymal stem cell. 762 A. Oseni et al.

Research paper thumbnail of Optimization of chondrocyte isolation and characterization for large-scale cartilage tissue engineering

Journal of Surgical Research, 2013

Harvest Nasoseptal cartilage Dispase Hyaluronidase Pronase Collagenase II a b s t r a c t Backgro... more Harvest Nasoseptal cartilage Dispase Hyaluronidase Pronase Collagenase II a b s t r a c t Background: Advancements in cartilage tissue engineering have the potential to ameliorate facial and joint reconstructive surgery as we know it. The translation of in vitro models of cartilage regeneration into clinical scenarios is the next phase of cartilage tissue engineering research. To engineer larger, more robust, and clinical relevant constructs, a great number of viable chondrocytic cells are needed. However, there is a paucity of literature concerning the most favorable method of chondrocyte isolation. Isolation methods are inconsistent, resulting in small yields and poor cell quality, and thus unreliable neocartilage formation. This study aimed to optimize the chondrocyte isolation protocol to give a maximum yield with optimal cell viability for the engineering of large cartilaginous constructs such as the human nose and ear. Methods: We employed several enzymes (pronase, dispase, hyaluronidase, and collagenase), enzyme concentrations, and digest lengths to digest freshly harvested ovine nasoseptal cartilage. We used automated trypan blue live/dead staining, immunofluorescent labeling of CD44, collagenase II, collagenase I, and Aggrecan, and alamarBlue to assess cell yield and viability. Results: Incubation length in enzymatic solutions had the greatest effect on cell viability, whereas concentrations of enzymes had a lesser effect. Isolated cells maintained their expression of chondrocyte-specific cell surface markers.

Research paper thumbnail of Nasal Reconstruction Using Tissue Engineered Constructs

Nasal Reconstruction Using Tissue Engineered Constructs

Annals of Plastic Surgery, 2013

Currently, the gold standard for reconstruction after rhinectomy or severe trauma to the nose, in... more Currently, the gold standard for reconstruction after rhinectomy or severe trauma to the nose, includes transposition of autologous mucosal flaps plus autologous cartilage grating and coverage using a skin flap. Difficulties with this approach arise where; cartilage and mucosa harvested from autologous donor sites is insufficient to achieve a passable aesthetic and functional reconstruction. Skin flaps are often bulky, poor color matches with hair follicles that reduce the aesthetic quality of the reconstruction. We suggest that tissue engineering could be a source of functional replacement tissues for nasal reconstructive surgery. However, the advancement of such an approach is dependent on the dissemination of scientific information into the clinical community, regarding the engineering of tissues such as mucosa, skin, and cartilage. This paper therefore reviews how the tissue engineering strategies available for producing clinically viable tissues could help resolve issues around reconstructing the human nose.

Research paper thumbnail of The application of POSS nanostructures in cartilage tissue engineering: the chondrocyte response to nanoscale geometry

The application of POSS nanostructures in cartilage tissue engineering: the chondrocyte response to nanoscale geometry

Journal of Tissue Engineering and Regenerative Medicine, 2013

Despite extensive research into cartilage tissue engineering (CTE), there is still no scaffold id... more Despite extensive research into cartilage tissue engineering (CTE), there is still no scaffold ideal for clinical applications. Various synthetic and natural polymers have been investigated in vitro and in vivo, but none have reached widespread clinical use. The authors investigate the potential of POSS-PCU, a synthetic nanocomposite polymer, for use in CTE. POSS-PCU is modified with silsesquioxane nanostructures that improve its biological and physical properties. The ability of POSS-PCU to support the growth of ovine nasoseptal chondrocytes was evaluated against a polymer widely used in CTE, polycaprolactone (PCL). Scaffolds with varied concentrations of the POSS molecule were also synthesized to investigate their effect on chondrocyte growth. Chondrocytes were seeded onto scaffold disks (PCU negative control; POSS-PCU 2%, 4%, 6%, 8%; PCL). Cytocompatibilty was evaluated using cell viability, total DNA, collagen and GAG assays. Chondrocytes cultured on POSS-PCU (2% POSS) scaffolds had significantly higher viability than PCL scaffolds (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). Total DNA, collagen and sGAG protein were also greater on POSS-PCU scaffolds compared with PCL (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05). POSS-PCU (6% and 8% POSS) had improved viability and proliferation over an 18 day culture period compared with 2% and 4% POSS-PCU (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). Increasing the percentage of POSS in the scaffolds increased the size of the pores found in the scaffolds (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). POSS-PCU has excellent potential for use in CTE. It supports the growth of chondrocytes in vitro and the POSS modification significantly enhances the growth and proliferation of nasoseptal chondrocytes compared with traditional scaffolds such as PCL. Copyright © 2013 John Wiley &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Sons, Ltd.