Adil Elgarrai - Academia.edu (original) (raw)
Papers by Adil Elgarrai
African Journal of Biotechnology, Dec 29, 2008
Asian Pacific Journal of Reproduction, 2018
Objective: To determine the effects of Calotropis procera latex on pubertal traits of immature ma... more Objective: To determine the effects of Calotropis procera latex on pubertal traits of immature male Wistar rats. Methods: A total of 30 immature male Wistar rats aged 3 weeks old were grouped randomly into 5 groups: group A (control group) was offered distilled water as a placebo; group B was daily oral dosed with suspension of Calotropis procera latex at a dose rate of 5 mg/kg BW, group C 10 mg/kg BW; group D 15 mg/kg BW and group E 20 mg/ kg BW. The rats were weighed daily to adjust the dose and record the BW changes, and the treatment continued for 4 weeks; thereafter, rats were sacrificed. Serum samples (n=30) were collected from all rats and kept frozen until assayed for reproductive hormones. Furthermore, the testes and epididymae were weighed; epididymal sperms were counted; sperm motility & abnormality were estimated; and histopathological sections of the testes were prepared. Results: The results of this study showed that oral dosing of immature male rats with Calotropis procera latex at doses rate 10 mg/kg BW significantly (P<0.05) reduced the growth rate, BW, testicular & epididymal weights, the level of most of reproductive hormones as well as the sperm traits examined; however, it significantly (P<0.05) augmented the abnormalities of spermatozoa and the seminiferous epithelium. Conclusions: Latex of Calotropis procera contains substances that have anti-androgenic activities and/or endocrine disrupting effects. If these substances are purified and identified, they can be used as male contraceptives.
PubMed, Dec 1, 1995
The influence of increasing the physical electrofusion parameters, direct current (DC) pulse stre... more The influence of increasing the physical electrofusion parameters, direct current (DC) pulse strength, pulse duration, pulse number, alternating current (AC) voltage and alignment time, in electrolytes on the rates of fusion, degeneration and development of zona-free mouse 2-cell embryos were examined. Furthermore, the effects of physiological saline and mannitol as fusion media and various mouse strains were also evaluated. Dulbecco's phosphate-buffered saline (PBS) supplemented with 10% fetal calf serum was used as the main fusion solution. A significant increase in the rate of fusion (P < 0.05) was obtained by increasing pulse strength from 30 to 300 V/mm. The embryos fused at the pulse strengths of 30 to 70 V/mm had significantly higher development rates to blastocysts compared with those fused at 100 to 300 V/mm (P < 0.05). There were no significant differences in the rates of fusion, degeneration and development to blastocysts when the pulse duration was increased from 30 to 90 microseconds. Although fusion rates were increased (P < 0.05) by increasing the pulse number up to 4, a significant decrease (P < 0.05) in development to blastocysts was observed when the pulse number was 5. Application of AC voltage prior to the DC pulse tended to increase the fusion rate (89.2-93.8%), compared with fusion with the DC pulse only (75.0%). Prolongation of alignment time from 5 to 15 sec had no effect on the fusion rate. Under the optimum conditions (2 pulses of DC of 70 V/mm, 70 microseconds pulse duration and AC of 5 V/mm for 5 sec), no significant difference was obtained in the fusion and development rates in different mouse strains, nor were fusion and development rates significantly different among PBS, physiological saline and mannitol solutions (P > 0.05).
Journal of Veterinary Medicine and Animal Production, Apr 6, 2014
This study was carried out to examine treatment of repeat breeder (RB) dairy cows with diluted Po... more This study was carried out to examine treatment of repeat breeder (RB) dairy cows with diluted Povidone iodine (2%). Sixty cross-bred dairy cows suffer repeat breeding were used to investigate the efficacy of intra-uterine infusion of diluted Pvidone iodine for treatment of repeat breeding in dairy cows. Uterine swabs were collected from uteri of the candidate cows prior treatment. After bacterial culture the uteri of the selected cows were found mildly infected (subclinical endometritis). They were then divided randomly into three equal groups. Group A (n = 20 cows) was intra-uterine infused with 2% Povidone iodine 6 hours pre-insemination. Group B (n = 20 cows) was infused with the same solution 6 hours post-insemination. Group C (n = 20 cows) was left without treatment to serve as control. The potency and efficacy of diluted Povidone iodine to cure repeat breeding in dairy cows was evaluated by studying three parameters, the days open (DO), rate of service per conception and the calving interval (CI). The results of this study revealed that, the DO, rate of service per conception and CI were significantly (P<0.001) reduced by intra-uterine infusion of diluted Povidone solution 6 hours pre and post insemination as compared to the control. It is concluded that, the repeat breeding (RB) syndrome in cross-bred dairy cows is most likely to be due to sub-clinical endometritis of the reproductive tract as well as deficiency of Iodine. Furthermore, intra-uterine infusion of 2% Povidone iodine can be used successfully to improve the reproductive efficiency of RB dairy cows.
IOSR Journal of Agriculture and Veterinary Science, 2014
Asian Pacific Journal of Reproduction, May 1, 2016
Asian Pacific Journal of Reproduction, Sep 1, 2016
Abstract Objective To investigate the effects of supplementation of potassium bromate (KBrO 3 ) t... more Abstract Objective To investigate the effects of supplementation of potassium bromate (KBrO 3 ) to drinking water on the growth rate, pubertal weight, testis & epididymal weights and testicular histology of growing male rats. Methods Thirty male Wizard rat [age = 21 d, mean BW = (40.0 ± 5.3) g] were used. The rats were grouped into 5 treatment groups each group consist of 6 rats. T 1 was offered drinking water supplemented with 100 mg, T 2 200 mg, T 3 300 mg, T 4 400 mg/L KBrO 3 for the duration of the experiment, while the control group was offered KBrO 3 free water. The BW weights were taken weekly. Eight weeks after treatment the rats were sacrificed, testes & epididymae were excised and weighted. The testes were fixed and histopathological sections of 5–6 μm were made, stained with H & E and examined under light microscope. Results The results showed that the growth rate, pubertal weight, testes & epididymal weights and testicular histology of growing male rats were significantly ( P 3 supplementation to drinking water. The growth rate of the control group and T 1 (100 mg/L KBrO 3 ) fit well a sigmoid pattern growth curve and no differences in their growth rates were recorded, while the sigmoid pattern of the growth curves of treatment T 2 , T 3 and T 4 was disrupted. No difference ( P > 0.05) in BW between the control and T 1 was recorded. However, it is clear that T 2 , T 3 and T 4 significantly ( P P 3 to drinking water caused serious changes in testicular tissue of the rats. The treatment rats' testes had distorted or even collapsed seminiferous tubules and narrow interstitial spaces. Upon magnification the seminiferous tubules appeared very narrow, mostly devoid of spermatogenesis and with no sperms. Conclusion Exposure of prepubertal rats to KBrO 3 retards their growth, causes testicular hypoplasia and impairs spermatogenesis, which is a predictor of infertility or even sterility in the future.
Animal Reproduction Science, Aug 1, 1997
Pronuclear stage embryos with intact (ZI), slit (ZS) or completely removed (ZF) zona pellucida we... more Pronuclear stage embryos with intact (ZI), slit (ZS) or completely removed (ZF) zona pellucida were encapsulated with an artificial zona pellucida (AZP) made of 1.5% sodium alginate. Embryos were cultured in KSOM medium with or without protein and their development in vitro to the blastocyst stage was recorded. AZP significantly (P < 0.05) improved the development of embryos to the blastocyst stage regardless of the presence of the natural zona pellucida. The encapsulated embryos developed at a higher rate (P < 0.05) in the absence of protein as compared with non-encapsulated embryos. Furthermore, the cell contacts at the 4-cell stage were significantly improved (P < 0.05) with encapsulation. AZP improved (P < 0.05) the development of pronuclear stage embryos with a slit zona pellucida to morula and blastocyst stages as compared with ZS embryos. It is concluded that AZP improves the in vitro development of pronuclear stage embryos with intact or completely removed zona pellucida as well as micromanipulated embryos to the blastocyst stage.
African Journal of Biotechnology, Aug 31, 2007
African Journal of Biotechnology, Dec 29, 2008
IOSR Journal of Dental and Medical Sciences, 2014
Journal of Veterinary Medical Science, 1997
Reproduction, Fertility and Development, 2006
IOSR Journal of Agriculture and Veterinary Science, 2014
Japanese Journal of Veterinary Research, Nov 30, 1998
African Journal of Biotechnology, 2011
IOSR Journal of Agriculture and Veterinary Science, 2013
Journal of Health Informatics in Developing Countries, 2018
Background: Saudization achieved limited success in private Saudi firms; nevertheless, it can ach... more Background: Saudization achieved limited success in private Saudi firms; nevertheless, it can achieve great success in the healthcare sector if there is sustainable graduation and training of Saudi healthcare-providers. Objectives: This study analyzed the structure of the healthcare workforce of some healthcare institutions in Najran city to know the rate of Saudization in the healthcare sector, the healthcare-workers qualification, healthcare-workers mean age, healthcare-workers years of expertise and the rate of hiring of Saudi female healthcare-givers. Methods: Cross sectional data of healthcare-workers from some health institutions in Najran city were collected by a simple questionnaire composed of personal data (such as age, gender, nationality, religion, qualification, major specialization and expertise in years). The questionnaire was distributed to 700 health working staff and the response rate was 73.6% (515 correctly filled questionnaire). Results: This study revealed that...
African Journal of Agricultural Research, Jul 31, 2008
African Journal of Biotechnology, Dec 29, 2008
Asian Pacific Journal of Reproduction, 2018
Objective: To determine the effects of Calotropis procera latex on pubertal traits of immature ma... more Objective: To determine the effects of Calotropis procera latex on pubertal traits of immature male Wistar rats. Methods: A total of 30 immature male Wistar rats aged 3 weeks old were grouped randomly into 5 groups: group A (control group) was offered distilled water as a placebo; group B was daily oral dosed with suspension of Calotropis procera latex at a dose rate of 5 mg/kg BW, group C 10 mg/kg BW; group D 15 mg/kg BW and group E 20 mg/ kg BW. The rats were weighed daily to adjust the dose and record the BW changes, and the treatment continued for 4 weeks; thereafter, rats were sacrificed. Serum samples (n=30) were collected from all rats and kept frozen until assayed for reproductive hormones. Furthermore, the testes and epididymae were weighed; epididymal sperms were counted; sperm motility & abnormality were estimated; and histopathological sections of the testes were prepared. Results: The results of this study showed that oral dosing of immature male rats with Calotropis procera latex at doses rate 10 mg/kg BW significantly (P<0.05) reduced the growth rate, BW, testicular & epididymal weights, the level of most of reproductive hormones as well as the sperm traits examined; however, it significantly (P<0.05) augmented the abnormalities of spermatozoa and the seminiferous epithelium. Conclusions: Latex of Calotropis procera contains substances that have anti-androgenic activities and/or endocrine disrupting effects. If these substances are purified and identified, they can be used as male contraceptives.
PubMed, Dec 1, 1995
The influence of increasing the physical electrofusion parameters, direct current (DC) pulse stre... more The influence of increasing the physical electrofusion parameters, direct current (DC) pulse strength, pulse duration, pulse number, alternating current (AC) voltage and alignment time, in electrolytes on the rates of fusion, degeneration and development of zona-free mouse 2-cell embryos were examined. Furthermore, the effects of physiological saline and mannitol as fusion media and various mouse strains were also evaluated. Dulbecco's phosphate-buffered saline (PBS) supplemented with 10% fetal calf serum was used as the main fusion solution. A significant increase in the rate of fusion (P < 0.05) was obtained by increasing pulse strength from 30 to 300 V/mm. The embryos fused at the pulse strengths of 30 to 70 V/mm had significantly higher development rates to blastocysts compared with those fused at 100 to 300 V/mm (P < 0.05). There were no significant differences in the rates of fusion, degeneration and development to blastocysts when the pulse duration was increased from 30 to 90 microseconds. Although fusion rates were increased (P < 0.05) by increasing the pulse number up to 4, a significant decrease (P < 0.05) in development to blastocysts was observed when the pulse number was 5. Application of AC voltage prior to the DC pulse tended to increase the fusion rate (89.2-93.8%), compared with fusion with the DC pulse only (75.0%). Prolongation of alignment time from 5 to 15 sec had no effect on the fusion rate. Under the optimum conditions (2 pulses of DC of 70 V/mm, 70 microseconds pulse duration and AC of 5 V/mm for 5 sec), no significant difference was obtained in the fusion and development rates in different mouse strains, nor were fusion and development rates significantly different among PBS, physiological saline and mannitol solutions (P > 0.05).
Journal of Veterinary Medicine and Animal Production, Apr 6, 2014
This study was carried out to examine treatment of repeat breeder (RB) dairy cows with diluted Po... more This study was carried out to examine treatment of repeat breeder (RB) dairy cows with diluted Povidone iodine (2%). Sixty cross-bred dairy cows suffer repeat breeding were used to investigate the efficacy of intra-uterine infusion of diluted Pvidone iodine for treatment of repeat breeding in dairy cows. Uterine swabs were collected from uteri of the candidate cows prior treatment. After bacterial culture the uteri of the selected cows were found mildly infected (subclinical endometritis). They were then divided randomly into three equal groups. Group A (n = 20 cows) was intra-uterine infused with 2% Povidone iodine 6 hours pre-insemination. Group B (n = 20 cows) was infused with the same solution 6 hours post-insemination. Group C (n = 20 cows) was left without treatment to serve as control. The potency and efficacy of diluted Povidone iodine to cure repeat breeding in dairy cows was evaluated by studying three parameters, the days open (DO), rate of service per conception and the calving interval (CI). The results of this study revealed that, the DO, rate of service per conception and CI were significantly (P<0.001) reduced by intra-uterine infusion of diluted Povidone solution 6 hours pre and post insemination as compared to the control. It is concluded that, the repeat breeding (RB) syndrome in cross-bred dairy cows is most likely to be due to sub-clinical endometritis of the reproductive tract as well as deficiency of Iodine. Furthermore, intra-uterine infusion of 2% Povidone iodine can be used successfully to improve the reproductive efficiency of RB dairy cows.
IOSR Journal of Agriculture and Veterinary Science, 2014
Asian Pacific Journal of Reproduction, May 1, 2016
Asian Pacific Journal of Reproduction, Sep 1, 2016
Abstract Objective To investigate the effects of supplementation of potassium bromate (KBrO 3 ) t... more Abstract Objective To investigate the effects of supplementation of potassium bromate (KBrO 3 ) to drinking water on the growth rate, pubertal weight, testis & epididymal weights and testicular histology of growing male rats. Methods Thirty male Wizard rat [age = 21 d, mean BW = (40.0 ± 5.3) g] were used. The rats were grouped into 5 treatment groups each group consist of 6 rats. T 1 was offered drinking water supplemented with 100 mg, T 2 200 mg, T 3 300 mg, T 4 400 mg/L KBrO 3 for the duration of the experiment, while the control group was offered KBrO 3 free water. The BW weights were taken weekly. Eight weeks after treatment the rats were sacrificed, testes & epididymae were excised and weighted. The testes were fixed and histopathological sections of 5–6 μm were made, stained with H & E and examined under light microscope. Results The results showed that the growth rate, pubertal weight, testes & epididymal weights and testicular histology of growing male rats were significantly ( P 3 supplementation to drinking water. The growth rate of the control group and T 1 (100 mg/L KBrO 3 ) fit well a sigmoid pattern growth curve and no differences in their growth rates were recorded, while the sigmoid pattern of the growth curves of treatment T 2 , T 3 and T 4 was disrupted. No difference ( P > 0.05) in BW between the control and T 1 was recorded. However, it is clear that T 2 , T 3 and T 4 significantly ( P P 3 to drinking water caused serious changes in testicular tissue of the rats. The treatment rats' testes had distorted or even collapsed seminiferous tubules and narrow interstitial spaces. Upon magnification the seminiferous tubules appeared very narrow, mostly devoid of spermatogenesis and with no sperms. Conclusion Exposure of prepubertal rats to KBrO 3 retards their growth, causes testicular hypoplasia and impairs spermatogenesis, which is a predictor of infertility or even sterility in the future.
Animal Reproduction Science, Aug 1, 1997
Pronuclear stage embryos with intact (ZI), slit (ZS) or completely removed (ZF) zona pellucida we... more Pronuclear stage embryos with intact (ZI), slit (ZS) or completely removed (ZF) zona pellucida were encapsulated with an artificial zona pellucida (AZP) made of 1.5% sodium alginate. Embryos were cultured in KSOM medium with or without protein and their development in vitro to the blastocyst stage was recorded. AZP significantly (P < 0.05) improved the development of embryos to the blastocyst stage regardless of the presence of the natural zona pellucida. The encapsulated embryos developed at a higher rate (P < 0.05) in the absence of protein as compared with non-encapsulated embryos. Furthermore, the cell contacts at the 4-cell stage were significantly improved (P < 0.05) with encapsulation. AZP improved (P < 0.05) the development of pronuclear stage embryos with a slit zona pellucida to morula and blastocyst stages as compared with ZS embryos. It is concluded that AZP improves the in vitro development of pronuclear stage embryos with intact or completely removed zona pellucida as well as micromanipulated embryos to the blastocyst stage.
African Journal of Biotechnology, Aug 31, 2007
African Journal of Biotechnology, Dec 29, 2008
IOSR Journal of Dental and Medical Sciences, 2014
Journal of Veterinary Medical Science, 1997
Reproduction, Fertility and Development, 2006
IOSR Journal of Agriculture and Veterinary Science, 2014
Japanese Journal of Veterinary Research, Nov 30, 1998
African Journal of Biotechnology, 2011
IOSR Journal of Agriculture and Veterinary Science, 2013
Journal of Health Informatics in Developing Countries, 2018
Background: Saudization achieved limited success in private Saudi firms; nevertheless, it can ach... more Background: Saudization achieved limited success in private Saudi firms; nevertheless, it can achieve great success in the healthcare sector if there is sustainable graduation and training of Saudi healthcare-providers. Objectives: This study analyzed the structure of the healthcare workforce of some healthcare institutions in Najran city to know the rate of Saudization in the healthcare sector, the healthcare-workers qualification, healthcare-workers mean age, healthcare-workers years of expertise and the rate of hiring of Saudi female healthcare-givers. Methods: Cross sectional data of healthcare-workers from some health institutions in Najran city were collected by a simple questionnaire composed of personal data (such as age, gender, nationality, religion, qualification, major specialization and expertise in years). The questionnaire was distributed to 700 health working staff and the response rate was 73.6% (515 correctly filled questionnaire). Results: This study revealed that...
African Journal of Agricultural Research, Jul 31, 2008