Adity Bose - Academia.edu (original) (raw)

Papers by Adity Bose

Department of Chemistry, Presidency University, 86/1, College Street, Kolkata-700 073, India<b... more Department of Chemistry, Presidency University, 86/1, College Street, Kolkata-700 073, India E-mail : adity.chem@presiuniv.ac.in, adityc17j@gmail.com Fax : 91-33-22413893 Manuscript received 12 August 2016, accepted 16 August 2016 Interactions of serum albumin (SA) with different drugs is an important topic of research in the field of biophysical chemistry. In this review 6-MP, an anticancer drug has been investigated with the help of different spectroscopic tools. The mode of interaction has been analyzed and finally docking with HSA has been given as an additional supporting information. From all the works it has been found that the main interaction of small 6-MP molecule with SAs has been primarily dynamic with a minor contribution from static component. Both thermodynamic parameters and docking studies revealed hydrophobic interaction to play the dominant role with domain II being the primary binding ...

The UV radiation is known as a potential environmental carcinogen which causes damages of macromo... more The UV radiation is known as a potential environmental carcinogen which causes damages of macromolecules like DNA, RNA, lipid, and protein inside the cell and eventually causes cell death. Persons who chronically exposed to UV radiation due to occupational or environmental exposure have a potential risk to develop cancer of the skin and internal organs. Polyphenols have a promising role in scavenging reactive oxygen species developed in our body due to a variety of exposure including radiation. In the present study, we are therefore evaluating the role of sinapic acid (SA), a polyphenol, in scavenging UV radiation-induced generation of reactive oxygen species and eventual cell death. We have studied the biological activity of SA as a potential antioxidant on human peripheral blood mononuclear cells taken from human volunteers. Methods Graded concentration of SA in different solvent mediums was applied to UV exposed peripheral blood mononuclear cells for evaluating the best effective...

ACS Food Science & Technology, 2022

Plant-derived flavonoids are an excellent option as a potential substitute to synthetic products ... more Plant-derived flavonoids are an excellent option as a potential substitute to synthetic products in food industries, and flavonoid−metal complexes exhibit better functional properties than free flavonoids. Here, an iron(II) complex of rutin, a dietary flavonoid, has been synthesized and characterized. A comparative assessment of the interaction of rutin and its iron(II) complex with the transport protein [bovine serum albumin (BSA)] and deoxyribonucleic acid (DNA) has been performed. The interaction process has been characterized using multispectroscopic techniques and has been further supported by theoretical studies. We have evidenced the alteration in the interacting mode of rutin with BSA upon complexation with Fe(II). Also, rutin interacts with DNA via minor groove binding, while the complex interacts with DNA through a mixed mode involving both groove binding and intercalative binding. Interestingly, the antioxidant activity of the metal complex has been found to be enhanced, and it also provides better protection against UV radiation-induced cell damage than rutin alone.

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2021

Dimethoxy-4-hydroxycinnamic acid commonly known as Sinapic acid is a well-known derivative of hyd... more Dimethoxy-4-hydroxycinnamic acid commonly known as Sinapic acid is a well-known derivative of hydroxycinnamic acids, is commonly present in human diet. Due to its wide variety of pharmacological activities like antioxidant, antimicrobial, anti-inflammatory, anticancer, and anti-anxiety, it has attracted much attention for the researchers. In our previous published work we have already analyzed the interaction between sinapic acid (SA) with a model transport protein. In this work our aim is to demonstrate a detailed investigation of the binding interaction between sinapic acid with another carrier of genetic information in a living cell, the DNA. Here we have used calf thymus DNA (ct-DNA) as a model. The binding characteristic of SA with ct-DNA was investigated by different spectroscopic and theoretical tools. The spectroscopic investigation revealed that quenching of intrinsic fluorescence of SA by ct-DNA occurs through dynamic quenching mechanism. The thermodynamic parameters established the involvement of hydrogen bonding and weak van der Waals forces in the interaction. Further, the circular dichroism, competitive binding experiment with ethidium bromide and potassium iodide quenching experiment suggested that SA possibly binds to the groove position of the ct-DNA. Finally, molecular docking analysis established the SA binds to minor groove position of ct-DNA in G-C rich region through hydrogen bonding interaction. Additionally, gel electrophoresis analysis has been performed to determine the protective efficacy of SA against UVB induced DNA damage and 50 μM of SA was found to protect the DNA from UVB induced damage. We hope that our study could provide the validation of SA on behalf of therapeutics and development of next generation therapeutic drug as well as designing new efficient drug molecule and methodology for the interaction study of the drug with DNA.

Journal of Herbal Medicine, 2020

serum albumin interaction in different media and its effective dose selection in radiation-induce... more serum albumin interaction in different media and its effective dose selection in radiation-induced cytotoxicity on human blood cells,

Materials Advances, 2020

This work deals with the synthesis of biomolecule-based monometallic Ag and Pd and bimetallic Ag–... more This work deals with the synthesis of biomolecule-based monometallic Ag and Pd and bimetallic Ag–Pd nanocomposites and their catalytic activity towards etherification reaction.

Food Chemistry, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

BioImpacts, 2019

Introduction: Flavonoids are widely used as dietary supplements, and thus, play a significant rol... more Introduction: Flavonoids are widely used as dietary supplements, and thus, play a significant role in the research field. In recent time, the interaction of flavonoid-metal complexes with serum albumin (SA) has widely been studied since the complexation poses a significant impact on biological activities. Additionally, the binding nature of flavonoids with SA gets modified in the presence of metal ions. Methods: In the present review, we studied the interaction of quercetin (Qu), a well-known flavonoid, and its Cu2+ complexes with SA to provide sufficient information about the beneficial role of metal-flavonoid complexes over free flavonoids. Results: Complexation with Cu(II) ion may alter the mode of binding of Qu with SAs. The strength of binding might be increased in the presence of Cu(II) as evidenced by the binding constant calculation. However, the drug binding site in bovine serum albumin (BSA) and human serum albumin (HSA) are not altered during the complexation process. Con...

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018

The crucial role of chemosensor for the immediate recognition of environment pollutant motivates ... more The crucial role of chemosensor for the immediate recognition of environment pollutant motivates the researchers to develop variety of sensing protocols. Of various chemosensory protocols, the colour change observed by the naked eye is considered to be a conceivable and on-site way to indicate the presence of an analyte. We herein report a colourimetric and commercially available absorption probe, Sinapic Acid (SA) that is completely ready to use for "on-site" visual determination of copper ions. The molecule, SA is well-known phenolic acid, often utilized for its antibacterial activity. In this work, for the first time, we are exploring its ability to work as an efficient Cu 2+ sensor. This sensor molecule selectively detected Cu 2+ ions by changing its colour from colourless to pink within detection limit of 64.5 nM, which is much lower than other reported sensor molecules and the suggested limit by World Health Organization (WHO) and U. S. Environmental Protection Agency (EPA) guidelines. The sensing mechanism was investigated through UV-vis and 1 H-NMR titration along with ESI-MS spectroscopy and further confirmed by DFT computational studies. Studies revealed the participation of hydroxyl group (-OH) and methoxy group (-OMe) of SA in complexation with Cu 2+. The binding stoichiometry of SA to Cu 2+ was found to be 1:2 through Job's plot and ESI-MS analysis. Importantly, paper strips of SA were prepared which could be used for a rapid "on-site" determination of Cu 2+ containing samples.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Jan 5, 2019

Cyclodextrins have a wide range of applications in different areas of drug delivery and pharmaceu... more Cyclodextrins have a wide range of applications in different areas of drug delivery and pharmaceutical industry due to their complexation ability and other versatile characteristics. Here we have studied the binding interactions of a small biologically important phenolic molecule, Thymol (Th), with both α and β cyclodextrins (CDs), which are well known drug delivery vehicles. Extent of encapsulation has been determined using several spectroscopic techniques. In fluorescence experiments, significant increase in fluorescence intensities have been discerned for both the CDs but there had been a much early saturation for αCD. Anisotropy experiments have been performed too and very surprisingly no appreciable increase in anisotropy value was observed in either case. Isothermal titration calorimetry (ITC) data, however, show signature of binding of Th with the βCD. These intriguing results were explained with the help of molecular docking and dynamics simulation studies. The docking calcu...

Journal of photochemistry and photobiology. B, Biology, 2018

The binding interaction of Rutin, a flavonoid, with model transport proteins, bovine serum albumi... more The binding interaction of Rutin, a flavonoid, with model transport proteins, bovine serum albumin (BSA) and human serum albumin (HSA), were investigated using different spectroscopic techniques, such as fluorescence, time-resolved single photon counting (TCSPC) and circular dichroism (CD) spectroscopy as well as molecular docking method. The emission studies revealed that the fluorescence quenching of BSA/HSA by Rutin occurred through a simultaneous static and dynamic quenching process, and we have evaluated both the quenching constants individually. The binding constants of Rutin-BSA and Rutin-HSA system were found to be 2.14 × 10 M and 2.36 × 10 M at 298 K respectively, which were quite high. Further, influence of some biologically significant metal ions (Ca, Zn and Mg) on binding of Rutin to BSA and HSA were also investigated. Thermodynamic parameters justified the involvement of hydrogen bonding and weak van der Waals forces in the interaction of Rutin with both BSA and HSA. Fu...

Spectroscopy Letters, 2017

The present work demonstrates the photophysical characterization of the interaction of two antiba... more The present work demonstrates the photophysical characterization of the interaction of two antibacterial and antimicrobial compounds, Thymol and its isomer, Carvacrol with different biomimetic micellar nanocavities having varying surface charge characteristics. These types of molecules are reported to be photoacids and this behavior is remarkably exhibited upon interaction with the macromolecular assemblies of micellar systems in presence of inorganic and organic quencher molecules (silver nitrate and diethylamine) and is manifested through significant modulations of the bimolecular quenching constant values.

AIP Conference Proceedings, 2008

Magnetic field effect combined with laser flash photolysis technique have been used to study the ... more Magnetic field effect combined with laser flash photolysis technique have been used to study the mechanism of interactions between two drug‐like quinone molecules, Menadione (1,4‐naphthoquinone, MQ) and 9, 10 Anthraquinone (AQ) with one of the DNA bases, ...

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The Journal of Physical Chemistry A, 2007

A derivative of phenazine, dibenzo[a,c]phenazine (DBPZ), can be used as a very good hydrogen-bond... more A derivative of phenazine, dibenzo[a,c]phenazine (DBPZ), can be used as a very good hydrogen-bonding probe unlike its parent phenazine molecule. Steady-state absorption and fluorescence studies reveal that DBPZ is completely insensitive to polarity of the medium. However, DBPZ can form a hydrogen bond very efficiently in its first excited singlet state. The extent of this excited-state hydrogen-bond formation depends both on size and on hydrogen-bond donor ability of the solvents. Time-resolved fluorescence studies and theoretical calculations also suggest that this hydrogen-bond formation is much more favorable in the excited state as compared to the ground state. In the excited state, the electron density is pushed toward the nitrogen atoms from the benzene rings, thereby increasing the dipole moment of the DBPZ molecule. Although the dipole moment of DBPZ increases upon photoexcitation, like other polarity probes, the molecule remains fully insensitive to the polarity of the interacting solvent. This unusual behavior of DBPZ as compared to simple phenazine and other polarity probes is due to the structure of the molecule. Hydrogen atoms at the 1 and 8 positions of DBPZ are sterically interacting with a lone pair of electrons on the proximate nitrogen atoms and make both of the nitrogen atoms inaccessible to solvent molecules. For this reason, DBPZ cannot sense the polarity of the medium. However, DBPZ can only sense solvents, those that have hydrogen with some electropositive nature, that is, the hydrogen-bond donating solvents. Hydrogen being the smallest among all elements can only interact with the lone pair of electrons of nitrogen atoms. Thus, DBPZ can act as a sensor for the hydrogen-bond donating solvents irrespective of their dielectrics.

The Journal of Physical Chemistry A, 2008

Laser flash photolysis and an external magnetic field have been used to study the interaction of ... more Laser flash photolysis and an external magnetic field have been used to study the interaction of two quinone molecules, namely, 9,10-anthraquinone (AQ) and 2-methyl 1,4-naphthoquinone, commonly known as menadione (MQ), with one of the DNA bases, guanine (G) and its nucleoside guanosine hydrate (dG). In organic homogeneous medium, it has been observed that G undergoes a predominant hydrogen (H) abstraction reaction with both the quinones while dG supports photoinduced electron transfer (PET) along with H abstraction. On the other hand, in SDS medium, G supports PET with AQ but not with MQ. However, behavior of dG remains unperturbed toward AQ and MQ with the change in medium. All of these observations have been explained on the basis of stabilization of radical ion pair and difference in size of the quinones, which can affect the distance of approach among the interacting molecules.

Science and Technology of Advanced Materials, 2008

The magnetic field effect (MFE) in conjunction with laser flash photolysis has been used for the ... more The magnetic field effect (MFE) in conjunction with laser flash photolysis has been used for the study of the interaction of one of the small drug like quinone molecules, 2-methyl, 1,4-naphthoquinone, commonly known as menadione (MQ), with one of the DNA bases, thymine (THN), and its corresponding nucleoside, thymidine (THDN), in acetonitrile (ACN) and sodium dodecylsulfate (SDS) micelles. It has been observed that THN undergoes electron transfer (ET) and hydrogen (H) abstraction with MQ, while THDN undergoes only H abstraction in both the media. However, our earlier studies showed that a purine base, adenine (ADN), and its nucleoside, 2-deoxyadenosine (ADS), undergo ET in ACN and H abstraction in SDS. Here we have attempted to explain the differences in the reactions of these DNA bases with MQ. We also reveal the crucial role of a sugar unit in altering the behavior of purine and pyrimidine bases with respect to ET and H abstraction.

Journal of Photochemistry and Photobiology A: Chemistry, 2007

This work illustrates a switchover of reaction mode on account of steric effect in different type... more This work illustrates a switchover of reaction mode on account of steric effect in different types of media. We have observed that in polar organic medium, 2-methyl-1,4-naphthoquinone (menadione, MQ) undergoes electron transfer (ET) with different amines, eg, triethylamine ...

Journal of Photochemistry and Photobiology A: Chemistry, 2009

Interaction between 2-methyl-1,4-naphthoquinone (MQ) and adenine (A) and 2′-deoxyadenosine (dA) r... more Interaction between 2-methyl-1,4-naphthoquinone (MQ) and adenine (A) and 2′-deoxyadenosine (dA) reveals interesting differences with respect to electron transfer (ET) and hydrogen abstraction (HA). In our case A and dA exhibited ET from different sites, which has been associated to ...

Journal of Molecular Liquids, 2014

We have studied the interactions of individual nucleoside 5′-monophosphates of all the five nucle... more We have studied the interactions of individual nucleoside 5′-monophosphates of all the five nucleic acid bases, adenosine 5′-monophosphate (AMP), guanosine 5′-monophosphate (GMP), thymidine 5′-monophosphate (TMP), cytidine 5′-monophosphate (CMP) and uridine 5′-monophosphate (UMP) with two quinones, 9,10anthraquinone (AQ) and 2-methyl 1,4-naphthoquinone (MQ) commonly known as menadione using laser flash photolysis in two different types of media. Although electron transfer (ET) in excited state is a well-known phenomenon in DNA with quinones, this work reveals a total failure of ET with these isolated 5′-monophosphate molecules in a homogeneous medium (acetonitrile/water), except GMP. Similar experiments in SDS micelles have been repeated but here even GMP failed to transfer electrons. We have attributed the failure of ET in homogeneous medium to a semi-circular conformation adopted by the 5′-monophosphates, which is assumed to pull back the electrons from the electron donor center, the nitrogenous base. In SDS micelles a rapid spin exchange within an abnormally short inter-radical distance has been considered to prevent ET.

Journal of Luminescence, 2006

The interactions between 9,10-anthraquinone (AQ) and different aromatic amines, N,N-dimethylanili... more The interactions between 9,10-anthraquinone (AQ) and different aromatic amines, N,N-dimethylaniline and 4,4′-bis (dimethylamino) diphenylmethane (DMDPM), have been studied using absorption, steady-state fluorescence, and laser flash photolysis techniques in organic ...