Adriaan Antonis - Academia.edu (original) (raw)

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Papers by Adriaan Antonis

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Tijdschrift voor diergeneeskunde, Jan 15, 2001

The aim of the experiment was to study whether bovine herpesvirus 1 (BHV1) marker vaccine batches... more The aim of the experiment was to study whether bovine herpesvirus 1 (BHV1) marker vaccine batches known to be contaminated with bovine virus diarrhoea virus (BVDV) type 1 could cause BVD in cattle. For this purpose, four groups of cattle were used. The first group (n = 4 calves, the positive control group), was vaccinated with vaccine from a batch contaminated with BVDV type 2. The second group (n = 4 calves, the negative control group), was vaccinated with vaccine from a batch that was not contaminated with BVDV. The third group (n = 39 calves), was vaccinated with a vaccine from one of four batches contaminated with BVDV type 1 (seronegative experimental group). The fourth group (n = 6 seropositive heifers), was vaccinated with a vaccine from one of three batches known to be contaminated with BVDV type 1. All cattle were vaccinated with an overdose of the BHV1 marker vaccine. At the start of the experiment, all calves except those from group 4 were seronegative for BVDV and BHV1. ...

Tools for enhancing employability & entrepreneurship Wijs met Water Inspirerende verhalen over de... more Tools for enhancing employability & entrepreneurship Wijs met Water Inspirerende verhalen over de waarde van water in het voedselsysteem Water is één van de meest essentiële natuurlijke hulpbronnen op aarde. De waarde van water wordt te vaak in Nederland onderschat. In dit document vragen wij meer aandacht voor de rol van water in de transitie naar een circulair voedselsysteem.

Veterinary Parasitology, 2016

Een grote uitdaging is het professionaliseren van de schapenhouderij en het verbeteren van het ec... more Een grote uitdaging is het professionaliseren van de schapenhouderij en het verbeteren van het economisch perspectief ervan, met behoud van het goede imago. Binnen de Publiek-Private Samenwerking Kleine Herkauwers-Schapen (PVE en EZ) is onderzoek uitgevoerd naar kansen voor van het verbinden van bestaande datastromen in de keten en het analyseren van data ter ondersteuning van de kwaliteitsslag die de sector wil maken.

Vooruitblik naar het symposium: "oude rassen, nieuwe kansen de weg naar succes". Een ui... more Vooruitblik naar het symposium: "oude rassen, nieuwe kansen de weg naar succes". Een uitgebreid programma en introductie van alle sprekers op het symposium maken er onderdeel van uit

Onderzoek naar kritische succesfactoren voor een laag antibioticumgebruik bij vleeskalveren. Wage... more Onderzoek naar kritische succesfactoren voor een laag antibioticumgebruik bij vleeskalveren. Wageningen Livestock Research, Rapport 1068A Samenvatting NL Op verzoek van SBK en het ministerie van Landbouw, Natuur en Voedselkwaliteit is onderzoek uitgevoerd naar kritische succesfactoren voor een laag antibioticumgebruik bij vleeskalveren. Binnen bestaande databases zijn de relaties van bedrijfs-en koppelkenmerken met het antibioticumgebruik geanalyseerd. Via een aanvullende enquête zijn verschillen in technische en sociale (ondernemers-) factoren tussen structurele laag-en hooggebruikers onderzocht. Summary UK Commissioned by SBK and the Ministry of Agriculture, Nature and Food Quality, research is carried out to identify critical success factors for low antibiotic use in veal calves. By analysis of sectoral databases, associations between farm and stock characteristics and antibiotics use are determined. With the results of an additional survey, differences in technical and social factors between structural low and high users of antibiotics are examined. Dit rapport is gratis te downloaden op https://doi.org/10.18174/427965 of op www.wur.nl/livestock-research (onder Wageningen Livestock Research publicaties).

Bioengineered Bugs, 2011

View related articles Citing articles: 3 View citing articles ©2 0 1 1 L a n d e s B i o s c i e ... more View related articles Citing articles: 3 View citing articles ©2 0 1 1 L a n d e s B i o s c i e n c e. D o n o t d i s t r i b u t e .

Veterinary Microbiology, 2004

Veterinary vaccines are usually tested for the absence of contaminants. However, the quality cont... more Veterinary vaccines are usually tested for the absence of contaminants. However, the quality control does not always imply that vaccines are not contaminated as, for example, illustrated by the bovine herpes virus 1 (BHV1) vaccine used in The Netherlands in 1999 that contained a small amount of bovine viral diarrhoea virus (BVDV1). Thousands of cows were vaccinated with BHV1 vaccine batches, and the question arose as to whether these small amounts of BVDV1, most likely not detected with in vitro tests, could have infected cattle. More in general, the question was whether the outcome of the in vitro tests, i.e. the in vitro infectivity, was indicative for the infectivity for cattle, i.e. the in vivo infectivity. We therefore carried out in vitro experiments to determine the sensitivity of a BVDV1 isolation assay. In addition, we performed two animal experiments, in which we estimated the lowest dose needed to infect calves with BVDV1. We extrapolated the experimental in vitro and in vivo results from a tissue culture infectious dose (TCID50) to a cattle infectious dose (CID50). We observed a partial response in the calves inoculated with this dose: four out of six calves turned out to be infected. In the tissue culture test, all 20 samples tested negative. The response in vivo, however, was not significantly higher than the in vitro response, which implies that no difference in susceptibility was observed between the animal test and the tissue culture test. Based on the results in our experiments, some cattle may have been infected with BVDV1 after the application of the contaminated BHV1 vaccine during the vaccination campaign. The question remains that how many cattle received contaminated vaccine, and became infected with BVDV1.

Veterinary Microbiology, 2005

Our current knowledge of antigenic variability of the bovine respiratory syncytial virus (BRSV) i... more Our current knowledge of antigenic variability of the bovine respiratory syncytial virus (BRSV) is quite limited and is mainly dependent on the use of monoclonal antibodies (mAb). In this study, we present not only analysis of the antigenic, but also of the genetic variability of BRSV. Using a panel of BRSV-specific mAb we distinguished five main reactivity patterns, three of which corresponded to the previously established subgroups A, B and AB. A single viral strain yielded the fourth pattern, while four viral strains did not react with any of the used mAbs forming the fifth pattern. To investigate the genetic basis for the antigenic heterogeneity of the BRS virus G protein, DNA of 11 BRSV isolates was directly sequenced. The comparison of the obtained nucleotide or amino acid sequences to those BRSV strains present in the GenBank revealed 88.1-99.4% and 77.7-98.4% similarity, respectively. These results supported the previously stated suggestion to type BRSV isolates according to their genetic relationship. In order to introduce a rapid and simple method to study the genetic variability of BRSV, we utilized the restriction enzyme analysis of RT-PCR products derived from mRNAs corresponding to the most variable region of the BRSV glycoprotein G ectodomain. Using this restriction enzyme analysis we were able to identify genetic variability among BRSV isolates. The detected non-synonymous mutations led frequently to a change in digestion pattern and were predominantly located in two mucin-like regions of the G protein gene. A correlation has been found between grouping of isolates in the phylogenetic tree and their restriction patterns clustering together isolates with the same restriction profiles. However, viruses placed distant in the tree sharing the same restriction patterns were detected supposing that phylogenetic analysis should be necessary for BRSV typing. Thus, we propose to use DNA restriction polymorphism for a rapid detection of genetic variants among BRSV isolates circulating in cattle population and as a preliminary tool for their typing.

BMC veterinary research, Jan 13, 2017

Pasteurella multocida, Mannheimia haemolytica, Histophilus somni and Trueperella pyogenes are fou... more Pasteurella multocida, Mannheimia haemolytica, Histophilus somni and Trueperella pyogenes are four bacterial agents commonly associated with bovine respiratory disease (BRD). In this study a bacterial multiplex real-time PCR (the RespoCheck PCR) was evaluated for the detection in bronchoalveolar lavage fluid (BALF) of these four bacterial agents. The analytical sensitivity of the multiplex real-time PCR assay determined on purified DNA and on bacterial cells of the four target pathogens was one to ten fg DNA/assay and 4 × 10(-1) to 2 × 10(0) CFU/assay. The analytical specificity of the test was, as evaluated on a collection of 118 bacterial isolates, 98.3% for M. haemolytica and 100% for the other three target bacteria. A set of 160 BALF samples of calves originating from ten different herds with health problems related to BRD was examined with bacteriological methods and with the RespoCheck PCR. Using bacteriological examination as the gold standard, the diagnostic sensitivities an...

Een reactie op het artikel 'BVD-aanpak: preventie' in tijdschrift voor Diergeneeskunde 13... more Een reactie op het artikel 'BVD-aanpak: preventie' in tijdschrift voor Diergeneeskunde 130(2005)nr. 16, waarin gesteld wordt dat op een rundveebedrijf alle of nagenoeg alle dieren dienen te beschikken over afweerstoffen tegen het BVD-virus om schade door besmetting te voorkomen. De reactie geeft aan dat dit onnauwkeurig is verwoord

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Tijdschrift voor diergeneeskunde, 2005