Agnieszka Kwiatek - Academia.edu (original) (raw)

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Papers by Agnieszka Kwiatek

Journal of Virology, 2013

We constructed a phagemid consisting of the whole genome of the Neisseria gonorrhoeae bacteriopha... more We constructed a phagemid consisting of the whole genome of the Neisseria gonorrhoeae bacteriophage NgoΦ6 cloned into a pBluescript plasmid derivative lacking the f1 origin of replication (named pBS::Φ6). Escherichia coli cells harboring pBS::Φ6 were able to produce a biologically active phagemid, NgoΦ6fm, capable of infecting, integrating its DNA into the chromosome of, and producing progeny phagemids in, a variety of taxonomically distant Gram-negative bacteria, including E. coli , Haemophilus influenzae , Neisseria sicca , Pseudomonas sp., and Paracoccus methylutens . A derivative of pBS::Φ6 lacking the phage orf7 gene, a positional homolog of filamentous phage proteins that mediate the interaction between the phage and the bacterial pilus, was capable of producing phagemid particles that were able to infect E. coli , Haemophilus influenzae , N. sicca , Pseudomonas sp., and Paracoccus methylutens , indicating that NgoΦ6 infects cells of these species using a mechanism that does n...

Journal of Virology, 2013

We constructed a phagemid consisting of the whole genome of the Neisseria gonorrhoeae bacteriopha... more We constructed a phagemid consisting of the whole genome of the Neisseria gonorrhoeae bacteriophage NgoΦ6 cloned into a pBluescript plasmid derivative lacking the f1 origin of replication (named pBS::Φ6). Escherichia coli cells harboring pBS::Φ6 were able to produce a biologically active phagemid, NgoΦ6fm, capable of infecting, integrating its DNA into the chromosome of, and producing progeny phagemids in, a variety of taxonomically distant Gram-negative bacteria, including E. coli , Haemophilus influenzae , Neisseria sicca , Pseudomonas sp., and Paracoccus methylutens . A derivative of pBS::Φ6 lacking the phage orf7 gene, a positional homolog of filamentous phage proteins that mediate the interaction between the phage and the bacterial pilus, was capable of producing phagemid particles that were able to infect E. coli , Haemophilus influenzae , N. sicca , Pseudomonas sp., and Paracoccus methylutens , indicating that NgoΦ6 infects cells of these species using a mechanism that does n...