Ahad Hossain - Academia.edu (original) (raw)
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Papers by Ahad Hossain
Live cell surface functionalization has been increasingly explored in recent years because of its... more Live cell surface functionalization has been increasingly explored in recent years because of its emerging bio- medical and therapeutic applications. Known methods to functionalize live-cell surface includes time-consuming metabolic docking of non-natural functional groups on cell surfaces that are hard to use. Here, we report an easy and direct functionalization method for cell surface and cell-cell conjugation utilizing native phosphate groups on cell membranes. We used three-dimensional single-molecule localization microscopy to confirm that the chemical conjugate binds preferentially on the cell surface. We show an application of this method for live-cell imaging and enhancement of cell-cell interaction leading to increased T cell proliferation. Our simple and reversible cell-conjugation strategy would be widely useful to explore and optimize cell-cell contact-based proliferation for any cell-based therapeutic applications.
Live cell surface functionalization has been increasingly explored in recent years because of its... more Live cell surface functionalization has been increasingly explored in recent years because of its emerging bio- medical and therapeutic applications. Known methods to functionalize live-cell surface includes time-consuming metabolic docking of non-natural functional groups on cell surfaces that are hard to use. Here, we report an easy and direct functionalization method for cell surface and cell-cell conjugation utilizing native phosphate groups on cell membranes. We used three-dimensional single-molecule localization microscopy to confirm that the chemical conjugate binds preferentially on the cell surface. We show an application of this method for live-cell imaging and enhancement of cell-cell interaction leading to increased T cell proliferation. Our simple and reversible cell-conjugation strategy would be widely useful to explore and optimize cell-cell contact-based proliferation for any cell-based therapeutic applications.