Ahlem Ben Sassi - Academia.edu (original) (raw)
Papers by Ahlem Ben Sassi
Natural Product Research, 2021
La cytotoxicite de 30 derives cyclopentanones est etudiee in vitro sur culture clonogenique de le... more La cytotoxicite de 30 derives cyclopentanones est etudiee in vitro sur culture clonogenique de leucemie murine L1210. Les resultats sont compares a ceux obtenus avec les medicaments de referencee 17 derives se sont averes actifs sur les cellules L1210.
Journal of Food Measurement and Characterization, 2020
Brassica oleracea var. gongylodes, commonly used as food, is loaded with biochemicals that fend d... more Brassica oleracea var. gongylodes, commonly used as food, is loaded with biochemicals that fend diabetes, cancer, high cholesterol and it improves the general human health. The present study aimed at evaluating the antioxidant and antibacterial activities of leaves, flesh and peel extracts of B. oleracea var. gongylodes cultivated in Tunisia. Besides, biochemical, phenolic and nutritional composition was investigated. Phenolics and ascorbic acid were detected and quantified by high performance liquid chromatography (HPLC) method, while fatty acids and sugars were identified by gas chromatography flame ionization detector (GC-FID). Nutritional composition was determined for each plant part. Antioxidant activities were estimated by DPPH, ABTS and reducing power assays. Agar disk diffusion and micro-dilution methods were adopted to determine antibacterial activity. Leaves showed significantly high antiradical (EC50 = 0.26–0.32 mg/mL), antioxidant (EC50 = 0.46 mg/mL) and antibacterial (MIC = 0.625 mg/mL) activities. Chlorogenic acid, catechol, epigallocatechin and epicatechin 3-O-gallate were present in leaves. Isorhamnetin-3-O-glucoside was detected in peel and flesh. Gallic acid was present only in flesh. Peel showed higher ascorbic acid content (223.2 mg/100 g DW) than flesh and leaves. Hemicellulose, chlorophylls, proteins, Na, P and microelements amounts were significantly elevated in leaves. Sucrose is the main sugar in stem tissues. Linoleic, palmitic and oleic acids were the major fatty acids in this species. The present study provides important data on phytochemical, nutritional composition, bioactivities and emphasizes B. oleracea var. gongylodes as a potential nutraceutical and functional food. Moreover, this species can be employed as an alternative and complementary therapy for the running of related diseases of oxidative stress.
Journal of Herbs, Spices & Medicinal Plants, 2019
Asian Pacific Journal of Tropical Medicine, 2018
Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constitu... more Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The α-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside (pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene (29.21%), γ -terpinene (12.96%), 4-terpenyl acetate (12.18%) and α -pinene (5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems (IC50=0.68 mg/mL) and flowers (IC50=0.67 mg/mL) methanolic extracts and essential oil (IC50=0.72 mg/mL). Findings of α-glucosidase activity revealed that petroleum ether extracts of leaves and roots together with aerial parts oil showed a highest activity with IC50 of 0.044, 0.045 and 0.049 mg/mL, respectively. Conclusions: Observed antioxidant and α-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes.
Journal of Molecular Structure, 2019
Abstract Large series of novel N-bis-1,2,3-triazolo-linked-1,5-benzodiazepin-2-ones (BZD) have be... more Abstract Large series of novel N-bis-1,2,3-triazolo-linked-1,5-benzodiazepin-2-ones (BZD) have been synthesized under microwave irradiation through a Cu(I)-catalyzed double 1,3-dipolar alkyne-azide coupling reaction. This process is of considerable synthetic advantages in terms of time saving and remarkable yields. The chemical structures of the isolated compounds have been elucidated on the basis of extensive spectroscopic methods including 1D & 2D NMR, IR and HRMS. All the synthesized compounds have been evaluated for their antimicrobial and antioxidant activities. In vitro antimicrobial environment, almost all of compounds have shown an interesting activity. Among the dimers of 1,5-benzodiazepine-1,2,3-triazole compounds tested, the results revealed that the potent antibacterial was recorded to compounds 3g,l for Gram-positive (within MIC = 31.25 and 125 μg/mL), and 3h,k for Gram-negative (within MIC = 31.25 and 125 μg/mL). Besides, the results demonstrate that compounds 3h,k have demonstrated the strongest potential against all the tested fungus (within MIC = 62.5 and 125 μg/mL). The antioxidant evaluation indicated that most compounds exhibited a moderate to good biological activity.
Chemistry & Biodiversity, 2019
The purpose of this study was to identify the chemical composition and the antibacterial activity... more The purpose of this study was to identify the chemical composition and the antibacterial activity of the essential oils (EOs) extracted from the green tops of Daucus carota L. subsp. sativus (Hoffm.) Arcang. plants producing yellow roots (DcsYR) and those producing orange roots (DcsOR) and from two varieties of Foeniculum vulgare subsp. vulgare cultivated in Tunisia. Analyses revealed that the EOs from the two D. carota varieties were rich in constituents belonging to sesquiterpenes. Phenylpropanoids and non‐terpene derivatives were the most abundant classes of compounds in the EOs from the two varieties of F. vulgare, of which compositions were predominated by (E)‐anethole and p‐acetonylanisole. All the tested EOs were significantly more effective against Gram‐negative bacteria, and that obtained from var. azoricum was more active against the yeast Candida albicans than the reference drug. The EOs obtained from these by‐products showed indeed interesting potential to be promoted as natural antimicrobials in food preservation systems, as well as the possibility to be used in flavor industries.
![Research paper thumbnail of [Effects of the methanolic extract of Chrysanthemum trifurcatum (Desf.) Batt. and Trab. on rat duodenal motility]](https://attachments.academia-assets.com/84583269/thumbnails/1.jpg)
](Comptes Rendus Biologies, Mar 1, 2007
Data Revues 16310748 00110003 07002445, Apr 30, 2008
Comptes Rendus Chimie, 2008
The chemical composition of essential oil isolated from the flowerheads of Chrysanthemum trifurca... more The chemical composition of essential oil isolated from the flowerheads of Chrysanthemum trifurcatum (Desf.) Batt. and Trab. var. macrocephalum (viv.) Beg. (Asteraceae) by hydrodistillation was analysed by GC and GC/MS. A total of 56 compounds representing 97.48% of the oil were identified: limonene (20.89%), γ-terpinene (19.13%), 1,8-cineole (10.64%), β-pinene (8.77%), α-pinene (5.32%), 2-hexenal (4.85%), 4-terpenyl acetate (3.42%), β-myrcene (2.31%), germacrene-B
The Indian journal of medical research, 2008
Tunisian Chrysanthemum species are known to have medicinal activity and some of the species are u... more Tunisian Chrysanthemum species are known to have medicinal activity and some of the species are used in traditional medicine. We have earlier shown the use of C. trifurcatum flowerheads in Tunisian traditional medicine to treat constipation. In the present study we investigated the anti microbiol activity of four Tunision Chrysanthemum species. Different parts (flowers, leaves, stems, roots, leaves and flowers and leaves and stems) of four Tunisian Chrysanthemum species, were extracted with solvents of increasing polarity to obtain aqueous and organic extracts. These extracts were tested in vitro for their antimicrobial activity against 14 bacteria and four yeasts, using agar diffusion and microdilution methods. Activity was evaluated by measuring the zones of inhibition against the tested organisms and minimum inhibitory concentrations (MIC) were determined from the lowest concentrations of extracts to inhibit the growth of microorganisms. Cytotoxity and antiviral activities agains...
La Tunisie médicale, 2004
The cytotoxicity of 30 cyclopentanone derivatives was studied in vitro, in a clonogenic assay usi... more The cytotoxicity of 30 cyclopentanone derivatives was studied in vitro, in a clonogenic assay using murine leukemia L1210. Results are compared to those obtained with reference medicaments. 17 derivatives exhibit activities against L1210 cells.
Journal of Oleo Science, 2014
Pharmaceutical Biology, 2007
... Ahlem Ben Sassi, Fethia Harzallah-Skhiri and Mahjoub Aouni ... The authors are grateful to Pr... more ... Ahlem Ben Sassi, Fethia Harzallah-Skhiri and Mahjoub Aouni ... The authors are grateful to ProfessorRachid Chemli, Mr. Mohamed Ben Salah (Laboratoire de Pharmacognosie, Faculté de Pharmacie, Monastir, Tunisia), and Misses Dalila Haouas and Lilia Trabelsi (Laboratoire ...
Comptes Rendus Biologies, 2007
Brazilian Journal of Pharmaceutical Sciences, 2020
Natural Product Research, 2008
Fifteen species of Tunisian traditional medicinal plants, belonging to 10 families, were selected... more Fifteen species of Tunisian traditional medicinal plants, belonging to 10 families, were selected for this study. They were Inula viscosa (L.) Ait and Reichardia tingitana (L.) Roth ssp. discolor (Pom.) Batt. (Asteraceae), Mesembryanthemum cristallinum L. and M. nodiflorum L. (Aizoaceae), Arthrocnemum indicum (Willd.) Moq., Atriplex inflata Muell., A. parvifolia Lowe var. ifiniensis (Caball) Maire, and Salicornia fruticosa L. (Chenopodiaceae), Cistus monspeliensis L. (Cistaceae), Juniperus phoenicea L. (Cupressaceae), Erica multiflora L. (Ericaceae), Frankenia pulverulenta L. (Frankeniaceae), Hypericum crispum L. (Hypericaceae), Plantago coronopus L. ssp. eu-coronopus Pilger var. vulgaris G.G. (Plantaginaceae) and Zygophyllum album L. (Zygophyllaceae). Fifty extracts prepared from those plants were screened in order to assay their antiviral activity against Herpes simplex virus type 1 (HSV-1), using neutral red incorporation. Extracts from eight plants among these 15 showed some degree of antiviral activity, while the methanolic extract of E. multiflora was highly active with EC(50) of 132.6 microg mL(-1). These results corroborate that medicinal plants from Tunisia can be a rich source of potential antiviral compounds.
Natural Product Research, 2021
La cytotoxicite de 30 derives cyclopentanones est etudiee in vitro sur culture clonogenique de le... more La cytotoxicite de 30 derives cyclopentanones est etudiee in vitro sur culture clonogenique de leucemie murine L1210. Les resultats sont compares a ceux obtenus avec les medicaments de referencee 17 derives se sont averes actifs sur les cellules L1210.
Journal of Food Measurement and Characterization, 2020
Brassica oleracea var. gongylodes, commonly used as food, is loaded with biochemicals that fend d... more Brassica oleracea var. gongylodes, commonly used as food, is loaded with biochemicals that fend diabetes, cancer, high cholesterol and it improves the general human health. The present study aimed at evaluating the antioxidant and antibacterial activities of leaves, flesh and peel extracts of B. oleracea var. gongylodes cultivated in Tunisia. Besides, biochemical, phenolic and nutritional composition was investigated. Phenolics and ascorbic acid were detected and quantified by high performance liquid chromatography (HPLC) method, while fatty acids and sugars were identified by gas chromatography flame ionization detector (GC-FID). Nutritional composition was determined for each plant part. Antioxidant activities were estimated by DPPH, ABTS and reducing power assays. Agar disk diffusion and micro-dilution methods were adopted to determine antibacterial activity. Leaves showed significantly high antiradical (EC50 = 0.26–0.32 mg/mL), antioxidant (EC50 = 0.46 mg/mL) and antibacterial (MIC = 0.625 mg/mL) activities. Chlorogenic acid, catechol, epigallocatechin and epicatechin 3-O-gallate were present in leaves. Isorhamnetin-3-O-glucoside was detected in peel and flesh. Gallic acid was present only in flesh. Peel showed higher ascorbic acid content (223.2 mg/100 g DW) than flesh and leaves. Hemicellulose, chlorophylls, proteins, Na, P and microelements amounts were significantly elevated in leaves. Sucrose is the main sugar in stem tissues. Linoleic, palmitic and oleic acids were the major fatty acids in this species. The present study provides important data on phytochemical, nutritional composition, bioactivities and emphasizes B. oleracea var. gongylodes as a potential nutraceutical and functional food. Moreover, this species can be employed as an alternative and complementary therapy for the running of related diseases of oxidative stress.
Journal of Herbs, Spices & Medicinal Plants, 2019
Asian Pacific Journal of Tropical Medicine, 2018
Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constitu... more Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The α-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside (pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene (29.21%), γ -terpinene (12.96%), 4-terpenyl acetate (12.18%) and α -pinene (5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems (IC50=0.68 mg/mL) and flowers (IC50=0.67 mg/mL) methanolic extracts and essential oil (IC50=0.72 mg/mL). Findings of α-glucosidase activity revealed that petroleum ether extracts of leaves and roots together with aerial parts oil showed a highest activity with IC50 of 0.044, 0.045 and 0.049 mg/mL, respectively. Conclusions: Observed antioxidant and α-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes.
Journal of Molecular Structure, 2019
Abstract Large series of novel N-bis-1,2,3-triazolo-linked-1,5-benzodiazepin-2-ones (BZD) have be... more Abstract Large series of novel N-bis-1,2,3-triazolo-linked-1,5-benzodiazepin-2-ones (BZD) have been synthesized under microwave irradiation through a Cu(I)-catalyzed double 1,3-dipolar alkyne-azide coupling reaction. This process is of considerable synthetic advantages in terms of time saving and remarkable yields. The chemical structures of the isolated compounds have been elucidated on the basis of extensive spectroscopic methods including 1D & 2D NMR, IR and HRMS. All the synthesized compounds have been evaluated for their antimicrobial and antioxidant activities. In vitro antimicrobial environment, almost all of compounds have shown an interesting activity. Among the dimers of 1,5-benzodiazepine-1,2,3-triazole compounds tested, the results revealed that the potent antibacterial was recorded to compounds 3g,l for Gram-positive (within MIC = 31.25 and 125 μg/mL), and 3h,k for Gram-negative (within MIC = 31.25 and 125 μg/mL). Besides, the results demonstrate that compounds 3h,k have demonstrated the strongest potential against all the tested fungus (within MIC = 62.5 and 125 μg/mL). The antioxidant evaluation indicated that most compounds exhibited a moderate to good biological activity.
Chemistry & Biodiversity, 2019
The purpose of this study was to identify the chemical composition and the antibacterial activity... more The purpose of this study was to identify the chemical composition and the antibacterial activity of the essential oils (EOs) extracted from the green tops of Daucus carota L. subsp. sativus (Hoffm.) Arcang. plants producing yellow roots (DcsYR) and those producing orange roots (DcsOR) and from two varieties of Foeniculum vulgare subsp. vulgare cultivated in Tunisia. Analyses revealed that the EOs from the two D. carota varieties were rich in constituents belonging to sesquiterpenes. Phenylpropanoids and non‐terpene derivatives were the most abundant classes of compounds in the EOs from the two varieties of F. vulgare, of which compositions were predominated by (E)‐anethole and p‐acetonylanisole. All the tested EOs were significantly more effective against Gram‐negative bacteria, and that obtained from var. azoricum was more active against the yeast Candida albicans than the reference drug. The EOs obtained from these by‐products showed indeed interesting potential to be promoted as natural antimicrobials in food preservation systems, as well as the possibility to be used in flavor industries.
Comptes Rendus Biologies, Mar 1, 2007
Data Revues 16310748 00110003 07002445, Apr 30, 2008
Comptes Rendus Chimie, 2008
The chemical composition of essential oil isolated from the flowerheads of Chrysanthemum trifurca... more The chemical composition of essential oil isolated from the flowerheads of Chrysanthemum trifurcatum (Desf.) Batt. and Trab. var. macrocephalum (viv.) Beg. (Asteraceae) by hydrodistillation was analysed by GC and GC/MS. A total of 56 compounds representing 97.48% of the oil were identified: limonene (20.89%), γ-terpinene (19.13%), 1,8-cineole (10.64%), β-pinene (8.77%), α-pinene (5.32%), 2-hexenal (4.85%), 4-terpenyl acetate (3.42%), β-myrcene (2.31%), germacrene-B
The Indian journal of medical research, 2008
Tunisian Chrysanthemum species are known to have medicinal activity and some of the species are u... more Tunisian Chrysanthemum species are known to have medicinal activity and some of the species are used in traditional medicine. We have earlier shown the use of C. trifurcatum flowerheads in Tunisian traditional medicine to treat constipation. In the present study we investigated the anti microbiol activity of four Tunision Chrysanthemum species. Different parts (flowers, leaves, stems, roots, leaves and flowers and leaves and stems) of four Tunisian Chrysanthemum species, were extracted with solvents of increasing polarity to obtain aqueous and organic extracts. These extracts were tested in vitro for their antimicrobial activity against 14 bacteria and four yeasts, using agar diffusion and microdilution methods. Activity was evaluated by measuring the zones of inhibition against the tested organisms and minimum inhibitory concentrations (MIC) were determined from the lowest concentrations of extracts to inhibit the growth of microorganisms. Cytotoxity and antiviral activities agains...
La Tunisie médicale, 2004
The cytotoxicity of 30 cyclopentanone derivatives was studied in vitro, in a clonogenic assay usi... more The cytotoxicity of 30 cyclopentanone derivatives was studied in vitro, in a clonogenic assay using murine leukemia L1210. Results are compared to those obtained with reference medicaments. 17 derivatives exhibit activities against L1210 cells.
Journal of Oleo Science, 2014
Pharmaceutical Biology, 2007
... Ahlem Ben Sassi, Fethia Harzallah-Skhiri and Mahjoub Aouni ... The authors are grateful to Pr... more ... Ahlem Ben Sassi, Fethia Harzallah-Skhiri and Mahjoub Aouni ... The authors are grateful to ProfessorRachid Chemli, Mr. Mohamed Ben Salah (Laboratoire de Pharmacognosie, Faculté de Pharmacie, Monastir, Tunisia), and Misses Dalila Haouas and Lilia Trabelsi (Laboratoire ...
Comptes Rendus Biologies, 2007
Brazilian Journal of Pharmaceutical Sciences, 2020
Natural Product Research, 2008
Fifteen species of Tunisian traditional medicinal plants, belonging to 10 families, were selected... more Fifteen species of Tunisian traditional medicinal plants, belonging to 10 families, were selected for this study. They were Inula viscosa (L.) Ait and Reichardia tingitana (L.) Roth ssp. discolor (Pom.) Batt. (Asteraceae), Mesembryanthemum cristallinum L. and M. nodiflorum L. (Aizoaceae), Arthrocnemum indicum (Willd.) Moq., Atriplex inflata Muell., A. parvifolia Lowe var. ifiniensis (Caball) Maire, and Salicornia fruticosa L. (Chenopodiaceae), Cistus monspeliensis L. (Cistaceae), Juniperus phoenicea L. (Cupressaceae), Erica multiflora L. (Ericaceae), Frankenia pulverulenta L. (Frankeniaceae), Hypericum crispum L. (Hypericaceae), Plantago coronopus L. ssp. eu-coronopus Pilger var. vulgaris G.G. (Plantaginaceae) and Zygophyllum album L. (Zygophyllaceae). Fifty extracts prepared from those plants were screened in order to assay their antiviral activity against Herpes simplex virus type 1 (HSV-1), using neutral red incorporation. Extracts from eight plants among these 15 showed some degree of antiviral activity, while the methanolic extract of E. multiflora was highly active with EC(50) of 132.6 microg mL(-1). These results corroborate that medicinal plants from Tunisia can be a rich source of potential antiviral compounds.