Ahmad Kandil - Academia.edu (original) (raw)

Papers by Ahmad Kandil

Research paper thumbnail of Sun-Exposed versus Sun-Protected Cutaneous Basal Cell Carcinoma: Clinico-Pathological Profile and p16 Immunostaining

Diagnostics

Introduction: Although widespread, BCC is still relatively poorly understood in regards to pathog... more Introduction: Although widespread, BCC is still relatively poorly understood in regards to pathogenesis and prognosis, particularly the lesions formed on anatomical sites away from sun exposure. With the aim of deepening our understanding of the pathogenesis and clinico-pathological correlations of BCCs, we conducted this study. Methods: Tissue blocks and data of 52 Egyptian patients diagnosed with BCC were retrieved for clinical information and inclusion criteria, then re-examined histologically; p16 immunostaining was carried out and evaluated for analysis and comparison between the two groups, i.e., sun-exposed and sun-protected. Results: Sex, age, clinical suspicion, tumor size, recurrence status, and histologic variants did not show a significant difference between the sun-protected and sun-exposed groups; however, the mean ages recorded were 67.2 vs. 62.7 for the sun-protected and sun-exposed groups, respectively. A total of 52% of BCCs were positive for p16. The sun-protected...

Research paper thumbnail of Histopathological, immunohistochemical and biomolecular diagnosis of myocarditis due to Clostridium chauvoei in a bovine

Ciência Rural, 2015

The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with ... more The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with emphasis on the pathological findings, isolation and molecular identification associated with the in situ localization of C. chauvoei. The animal, a male Brangus bull with nine months of age, was found dead without prior clinical signs. Multifocal and coalescent areas of necrosis were observed in the myocardium. Rod cells in the cardiac muscle fibers were positive immunostaining for C. chauvoei, while this bacterium was also isolated and identified by polymerase chain reaction (PCR).

‘igure 1 - Myocarditis due to Clostridium chauvoei in cattle: A. Pericardial adhesion to the epicardium, associated with significant fibrin deposition. B. Dilatation of the right and left ventricles with pallor areas in the myocardium (arrows). A detailed view of the pale area is shown in the inset. C. Epicardium with marked diffuse fibrin deposition (asterisk) and marked diffuse neutrophils infiltration. HE, Obj.20. D. Coagulative necrosis of the myocardium (asterisk) surrounded by intact and degenerate neutrophils (arrow) HE, Obj.10. E. Numerous Gram-positive rods in the area of myocardial necrosis (arrows). Brown-modified Hopps stain, Obj.40. F. Immunohistochemistry demonstrating that the tissue is positive for Anti-C. chauvoei (arrow). Streptavidin-biotin peroxidase. Obj.40. a RS Se oe ee ee ee ee a, ee containing fibrin was observed in the thoracic cavity. In the heart, the pericardium was adhered to the parietal pleura and epicardium. Additionally, significant fibrin deposition (Figure 1A), marked dilatation of the right and left ventricles and multifocal areas of pallor measuring 0.5cm were found in the myocardium (Figure 1B). The lungs were slightly collapsed and A male Brangus bull, 9 months of age was referred to the Federal University of Rio Grande do Sul (UFRGS), where the necropsy was performed. The animal was found dead without prior clinical signs. The bovine belonged to a herd of 150 animals kept at a farm in the state of Rio Grande do Sul, Brazil, and had been vaccinated for foot and mouth disease in the week before death, but did not receive vaccines against

Research paper thumbnail of Nasal Polyp, Pathology, Sinonasal Carcinoma

Nasal Polyp, Pathology, Sinonasal Carcinoma

The Utility of Clinicopathological Correlation of Sinonasal Masses in a Tertiary Hospital

, Aug 3, 2021

Research paper thumbnail of Effect of Mesenchymal Stem Cell – Derived Extracellular Vesicles on Acetaminophen – Induced Nephrotoxicity in Adult Male Albino Rats

al-azhar medical journal, Dec 1, 2017

Background: Novel therapies are urgently needed to address the rising incidence and prevalence of... more Background: Novel therapies are urgently needed to address the rising incidence and prevalence of acute kidney injury (AKI) and chronic kidney disease (CKD). Mesenchymal stem cells (MSCs) can generate extracellular micro vesicles (EVs) with intrinsic protective or regenerative capacity. Objectives: Studying the possible role of EVs generated from bone marrow-derived mesenchymal stem cells (BM MSCs) in regeneration of kidney tissue in acetaminophen-induced renal failure (RF). Patients and Methods: Twenty four adult male albino rats of a local strain were chosen as an animal model for this study. They were divided into 4 equal groups: control, RF, RF received culture media, and RF received MSCs-derived EVs. Renal failure (RF) was induced by oral administration of acetaminophen. At the end of the experiment (24 days), blood samples were obtained for serum creatinine, urea, malodialdehyde (MDA) and tumor necrosis factor alpha (TNF-α). Animals were sacrificed, and kidney tissue was obtained for histopathological examination and immunohistochemical examination for BAX protein expression, in addition to BCL2 gene expression by real time (RT) PCR. Results: There was a significant decrease in BCL2 gene expression in addition to significant increases in serum creatinine, urea, MDA and TNF-α in acetaminophen-treated group. There was an increased BAX protein expression by immunohistochemistry in acetaminophen-treated group. There were significant increase in BCL2 gene expression, and significant decreases in serum creatinine, urea, MDA and TNF-α in MSCs-derived EVs-treated group. There was a decrease in BAX protein expression by immunohistochemistry in MSCs derived EVs-treated group. Conclusion: BM MSCs-derived (EVs) have a role in regeneration of kidney tissue in acetaminopheninduced renal failure through antioxidant, anti-inflammatory and anti-apoptotic mechanisms.

Research paper thumbnail of Behavior and neuropsychiatric changes in experimental chronic toxoplasmosis: Histopathological and immunohistochemical studies

Background: T. gondii is an intracellular protozoan parasite that can establish a latent infectio... more Background: T. gondii is an intracellular protozoan parasite that can establish a latent infection in the central nervous system that may develop into chronic inflammation resulting in neurobehavioral problems in the host. The processes behind these alterations are still largely mysterious. Objective: Detection of behavioral, histopathological and immunohistochemical changes in mice infected by T. gondii Me49 strain. Material and Methods: A total of 105 adult male Swiss albino mice were divided into 60 used for experimental infection, and 45 as control. Assessment of physical appearance was monitored for acute toxoplasmosis daily for three weeks post infection (PI). Correlation between behavior changes and the degree of infection was conducted by measuring histopathological (H&E and silver stain) and immunohistochemical (presence or absence of CD3, CD138 and caspase-3 immunoreactive cells) parameters weekly starting from 7th week to the 12th week PI. Results: Infected mice had neuro...

Research paper thumbnail of Immunohistochemical Expression of P16 in Cervical and Urinary Bladder Squamous Cell Carcinoma

Al-Azhar Medical Journal, 2021

Background: p16 is a tumor suppressor protein important in regulating cell cycle. In bladder canc... more Background: p16 is a tumor suppressor protein important in regulating cell cycle. In bladder cancer, p16 mutations are widespread and tend to be more prevalent in low-grade superficial tumors than in higher-grade invasive tumors. Objective: To evaluate expression of p16 in 25 cases of cervical squamous cell carcinoma and 25 cases of urinary bladder squamous cell carcinoma. Patients and methods: This study was carried out in the Histopathology Department of Al-Azhar University Hospitals and from some private laboratories during the period from August 2019 up to January 2020. H & E stained sections were prepared from paraffin blocks for revision of diagnosis. Immunohistochemical analysis was done on 4 micron sections of formalin-fixed paraffin-embedded tissue using the labeled streptavidinbiotin method after antigen retrieval. Antibodies against P16 were utilized on sections from paraffin blocks. CINtec Histology kit includes a two-step antibody method for detection of human p16 antib...

Research paper thumbnail of The Utility of Clinicopathological Correlation of Sinonasal Masses in a Tertiary Hospital

Journal of Evolution of Medical and Dental Sciences

BACKGROUND Sinonasal masses are commonly seen lesions in ENT practice with multiple aetiological ... more BACKGROUND Sinonasal masses are commonly seen lesions in ENT practice with multiple aetiological factors. We wanted to study the clinico-pathological features of nasal mass specimens in a tertiary hospital and evaluate the utility of routine histopathological examination of the surgically removed nasal tissue specimens. METHODS All the histologically examined specimens of patients clinically presenting with nasal or sinonasal lesions over a period of one year were retrospectively studied and analyzed. RESULTS Out of total 36 patients with masses, 21 were males (58.3 %) and 15 (41.7 %) were females. The age ranged from 10 to 75 years with a mean age of 31.4 years and maximum patients were in the third decade (37.5 %). The most common presenting clinical symptom was nasal obstruction that was seen in 33 patients (91.6 %). Computed tomography (CT) scan was done in 94.5 % of cases prior to the surgical intervention. One fifth of the sinonasal lesions were neoplastic as per histopatholog...

Research paper thumbnail of Sun-Exposed versus Sun-Protected Cutaneous Basal Cell Carcinoma: Clinico-Pathological Profile and p16 Immunostaining

Diagnostics

Introduction: Although widespread, BCC is still relatively poorly understood in regards to pathog... more Introduction: Although widespread, BCC is still relatively poorly understood in regards to pathogenesis and prognosis, particularly the lesions formed on anatomical sites away from sun exposure. With the aim of deepening our understanding of the pathogenesis and clinico-pathological correlations of BCCs, we conducted this study. Methods: Tissue blocks and data of 52 Egyptian patients diagnosed with BCC were retrieved for clinical information and inclusion criteria, then re-examined histologically; p16 immunostaining was carried out and evaluated for analysis and comparison between the two groups, i.e., sun-exposed and sun-protected. Results: Sex, age, clinical suspicion, tumor size, recurrence status, and histologic variants did not show a significant difference between the sun-protected and sun-exposed groups; however, the mean ages recorded were 67.2 vs. 62.7 for the sun-protected and sun-exposed groups, respectively. A total of 52% of BCCs were positive for p16. The sun-protected...

Research paper thumbnail of Histopathological, immunohistochemical and biomolecular diagnosis of myocarditis due to Clostridium chauvoei in a bovine

Ciência Rural, 2015

The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with ... more The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with emphasis on the pathological findings, isolation and molecular identification associated with the in situ localization of C. chauvoei. The animal, a male Brangus bull with nine months of age, was found dead without prior clinical signs. Multifocal and coalescent areas of necrosis were observed in the myocardium. Rod cells in the cardiac muscle fibers were positive immunostaining for C. chauvoei, while this bacterium was also isolated and identified by polymerase chain reaction (PCR).

‘igure 1 - Myocarditis due to Clostridium chauvoei in cattle: A. Pericardial adhesion to the epicardium, associated with significant fibrin deposition. B. Dilatation of the right and left ventricles with pallor areas in the myocardium (arrows). A detailed view of the pale area is shown in the inset. C. Epicardium with marked diffuse fibrin deposition (asterisk) and marked diffuse neutrophils infiltration. HE, Obj.20. D. Coagulative necrosis of the myocardium (asterisk) surrounded by intact and degenerate neutrophils (arrow) HE, Obj.10. E. Numerous Gram-positive rods in the area of myocardial necrosis (arrows). Brown-modified Hopps stain, Obj.40. F. Immunohistochemistry demonstrating that the tissue is positive for Anti-C. chauvoei (arrow). Streptavidin-biotin peroxidase. Obj.40. a RS Se oe ee ee ee ee a, ee containing fibrin was observed in the thoracic cavity. In the heart, the pericardium was adhered to the parietal pleura and epicardium. Additionally, significant fibrin deposition (Figure 1A), marked dilatation of the right and left ventricles and multifocal areas of pallor measuring 0.5cm were found in the myocardium (Figure 1B). The lungs were slightly collapsed and A male Brangus bull, 9 months of age was referred to the Federal University of Rio Grande do Sul (UFRGS), where the necropsy was performed. The animal was found dead without prior clinical signs. The bovine belonged to a herd of 150 animals kept at a farm in the state of Rio Grande do Sul, Brazil, and had been vaccinated for foot and mouth disease in the week before death, but did not receive vaccines against

Research paper thumbnail of Nasal Polyp, Pathology, Sinonasal Carcinoma

Nasal Polyp, Pathology, Sinonasal Carcinoma

The Utility of Clinicopathological Correlation of Sinonasal Masses in a Tertiary Hospital

, Aug 3, 2021

Research paper thumbnail of Effect of Mesenchymal Stem Cell – Derived Extracellular Vesicles on Acetaminophen – Induced Nephrotoxicity in Adult Male Albino Rats

al-azhar medical journal, Dec 1, 2017

Background: Novel therapies are urgently needed to address the rising incidence and prevalence of... more Background: Novel therapies are urgently needed to address the rising incidence and prevalence of acute kidney injury (AKI) and chronic kidney disease (CKD). Mesenchymal stem cells (MSCs) can generate extracellular micro vesicles (EVs) with intrinsic protective or regenerative capacity. Objectives: Studying the possible role of EVs generated from bone marrow-derived mesenchymal stem cells (BM MSCs) in regeneration of kidney tissue in acetaminophen-induced renal failure (RF). Patients and Methods: Twenty four adult male albino rats of a local strain were chosen as an animal model for this study. They were divided into 4 equal groups: control, RF, RF received culture media, and RF received MSCs-derived EVs. Renal failure (RF) was induced by oral administration of acetaminophen. At the end of the experiment (24 days), blood samples were obtained for serum creatinine, urea, malodialdehyde (MDA) and tumor necrosis factor alpha (TNF-α). Animals were sacrificed, and kidney tissue was obtained for histopathological examination and immunohistochemical examination for BAX protein expression, in addition to BCL2 gene expression by real time (RT) PCR. Results: There was a significant decrease in BCL2 gene expression in addition to significant increases in serum creatinine, urea, MDA and TNF-α in acetaminophen-treated group. There was an increased BAX protein expression by immunohistochemistry in acetaminophen-treated group. There were significant increase in BCL2 gene expression, and significant decreases in serum creatinine, urea, MDA and TNF-α in MSCs-derived EVs-treated group. There was a decrease in BAX protein expression by immunohistochemistry in MSCs derived EVs-treated group. Conclusion: BM MSCs-derived (EVs) have a role in regeneration of kidney tissue in acetaminopheninduced renal failure through antioxidant, anti-inflammatory and anti-apoptotic mechanisms.

Research paper thumbnail of Behavior and neuropsychiatric changes in experimental chronic toxoplasmosis: Histopathological and immunohistochemical studies

Background: T. gondii is an intracellular protozoan parasite that can establish a latent infectio... more Background: T. gondii is an intracellular protozoan parasite that can establish a latent infection in the central nervous system that may develop into chronic inflammation resulting in neurobehavioral problems in the host. The processes behind these alterations are still largely mysterious. Objective: Detection of behavioral, histopathological and immunohistochemical changes in mice infected by T. gondii Me49 strain. Material and Methods: A total of 105 adult male Swiss albino mice were divided into 60 used for experimental infection, and 45 as control. Assessment of physical appearance was monitored for acute toxoplasmosis daily for three weeks post infection (PI). Correlation between behavior changes and the degree of infection was conducted by measuring histopathological (H&E and silver stain) and immunohistochemical (presence or absence of CD3, CD138 and caspase-3 immunoreactive cells) parameters weekly starting from 7th week to the 12th week PI. Results: Infected mice had neuro...

Research paper thumbnail of Immunohistochemical Expression of P16 in Cervical and Urinary Bladder Squamous Cell Carcinoma

Al-Azhar Medical Journal, 2021

Background: p16 is a tumor suppressor protein important in regulating cell cycle. In bladder canc... more Background: p16 is a tumor suppressor protein important in regulating cell cycle. In bladder cancer, p16 mutations are widespread and tend to be more prevalent in low-grade superficial tumors than in higher-grade invasive tumors. Objective: To evaluate expression of p16 in 25 cases of cervical squamous cell carcinoma and 25 cases of urinary bladder squamous cell carcinoma. Patients and methods: This study was carried out in the Histopathology Department of Al-Azhar University Hospitals and from some private laboratories during the period from August 2019 up to January 2020. H & E stained sections were prepared from paraffin blocks for revision of diagnosis. Immunohistochemical analysis was done on 4 micron sections of formalin-fixed paraffin-embedded tissue using the labeled streptavidinbiotin method after antigen retrieval. Antibodies against P16 were utilized on sections from paraffin blocks. CINtec Histology kit includes a two-step antibody method for detection of human p16 antib...

Research paper thumbnail of The Utility of Clinicopathological Correlation of Sinonasal Masses in a Tertiary Hospital

Journal of Evolution of Medical and Dental Sciences

BACKGROUND Sinonasal masses are commonly seen lesions in ENT practice with multiple aetiological ... more BACKGROUND Sinonasal masses are commonly seen lesions in ENT practice with multiple aetiological factors. We wanted to study the clinico-pathological features of nasal mass specimens in a tertiary hospital and evaluate the utility of routine histopathological examination of the surgically removed nasal tissue specimens. METHODS All the histologically examined specimens of patients clinically presenting with nasal or sinonasal lesions over a period of one year were retrospectively studied and analyzed. RESULTS Out of total 36 patients with masses, 21 were males (58.3 %) and 15 (41.7 %) were females. The age ranged from 10 to 75 years with a mean age of 31.4 years and maximum patients were in the third decade (37.5 %). The most common presenting clinical symptom was nasal obstruction that was seen in 33 patients (91.6 %). Computed tomography (CT) scan was done in 94.5 % of cases prior to the surgical intervention. One fifth of the sinonasal lesions were neoplastic as per histopatholog...