Ahmed Dawod - Academia.edu (original) (raw)
Papers by Ahmed Dawod
Journal of Clinical Oncology, Jun 1, 2023
Frontiers in Oncology
BackgroundOvarian cancer (OC) is the deadliest gynecological cancer, often diagnosed at advanced ... more BackgroundOvarian cancer (OC) is the deadliest gynecological cancer, often diagnosed at advanced stages. A fast and accurate diagnostic method for early-stage OC is needed. The tumor marker gangliosides, GD2 and GD3, exhibit properties that make them ideal potential diagnostic biomarkers, but they have never before been quantified in OC. We investigated the diagnostic utility of GD2 and GD3 for diagnosis of all subtypes and stages of OC.MethodsThis retrospective study evaluated GD2 and GD3 expression in biobanked tissue and serum samples from patients with invasive epithelial OC, healthy donors, non-malignant gynecological conditions, and other cancers. GD2 and GD3 levels were evaluated in tissue samples by immunohistochemistry (n=299) and in two cohorts of serum samples by quantitative ELISA. A discovery cohort (n=379) showed feasibility of GD2 and GD3 quantitative ELISA for diagnosing OC, and a subsequent model cohort (n=200) was used to train and cross-validate a diagnostic model...
Journal of Current Veterinary Research
This study aimed to investigate the effect of climatic factors, including temperature-humidity in... more This study aimed to investigate the effect of climatic factors, including temperature-humidity index (THI) and rainfall level (RFL) regarding the parity on the incidence of mastitis, milk yield, and composition in Holstein cattle in Egypt. A total of 2496 lactating cows were used from different four dairy farms located in Egypt. One hundred dairy cows were selected from each dairy herd for detection of the effect of different climatic factors and parity on milk composition and somatic cell count (SCC).The data grouped according to parity into 1, 2, and >2 parity groups. Also, the animals grouped according to THI into low (<70), medium (70-80), and high (>80) THI level and regrouped according to RFL into low (<2 mm), medium (2-5 mm), and high (>5 mm) rainfall per month. The obtained results revealed an increase in daily milk yield, milk protein%, fat %, and SCC with the increase of parity. Also, high THI significantly (p≤ 0.05) decreased daily milk yield (29.45 kg) and increased SCC (415 × 103 cell/mL). In high THI and low RFL climatic conditions, multiparous cows were prone to the occurrence of clinical mastitis compared to primiparous ones (p≤ 0.001); especially during mid and late lactation stages. In conclusion, high THI and low RFL impaired dairy performance and increased the frequency of clinical mastitis.
Zagazig Veterinary Journal
This study was constructed to investigate the differences between three breeds of rabbits [New Ze... more This study was constructed to investigate the differences between three breeds of rabbits [New Zealand White (NZW), Rex and Egyptian Gabali (Al-Gabali)] under heat stress regarding growth traits, hematological, biochemical and immunological parameters, oxidative stress biomarkers as well as metabolic hormones. Thirty rabbits of each breed (4 weeks of age) were subjected to heat stress (32 ± 1°C, 70-80% relative humidity) in the summer season at the period from mid-July to the end of August 2019. Blood samples were collected at 10 th week of age. The results revealed that body weights of NZW and Rex were higher by 205 and 145 g, respectively than those of Al-Gabali as well as average daily gains of NZW and Rex were higher by 4.42 and 3.40 g/day, respectively compared with Al-Gabali (P <0.05). Average daily feed intake of Rex was lower by 7.65 g/day than those of NZW and 2.89 g/day compared with Al-Gabali (P <0.05) and they had the lowest feed to gain ratios (3.58±0.097). Aspartate aminotransferase, urea and creatinine were significantly lowered in the plasma of Al-Gabali compared with NZW and Rex whereas, Al-Gabali recorded higher total proteins (5.90±0.048 g/dL), total globulins (2.50±0.054 g/dL), antibody titer against sheep red blood cells (6.38±0.111) and superoxide dismutase (7.42±0.163 IU/L) than NZW (5.66±0.069 g/dL, 2.21±0.084 g/dL, 4.90±0.068 and 6.80±0.053 IU/L, respectively) and Rex (5.74±0.072 g/dL 2.25±0.095 g/dL, 4.25±0.095 and 6.88±0.050 IU/L, respectively). Al-Gabali bunnies had higher immunoglobulin G, immunoglobulin A, catalase and glutathione peroxidase than NZW and Rex. However, Al-Gabali recorded the lowest insulin (15.64±0.20 ng/mL), growth hormone (45.80±0.48 ng/mL) and triiodothyronine (136.20±2.15 ng/mL). In conclusion, Al-Gabali rabbits were better adapted to heat stress compared with NZW and Rex, which was reflected in the improvement of health, immunity and oxidative stress indicators as well as lower plasma metabolic hormones, but they didn't have the genetic potential for growth traits.
Journal of Current Veterinary Research
This study aimed to evaluation of the in vitro and in vivo acaricidal activity of the Ficus sycom... more This study aimed to evaluation of the in vitro and in vivo acaricidal activity of the Ficus sycomorus (F. sycomorus) against fur mites in rabbits. Methanol 70% was used to extract F. sycomorus extracts from either the leaf or stem bark. The in vitro acaricidal activity of F. sycomorus extracts was tested using 2 ml of each F. sycomorus extract at 0.125, 0.25, 0.5, and 1 mg/ml concentrations in each experimental Petri dish containing ten Sarcoptesscabiei (S. scabiei) mites/dish. Negative control mites were given distilled water, whereas positive control mites were given selamectin. The stereomicroscopic examination was then performed 30 minutes after incubation and was repeated hourly until 7 hours after incubation. The mortality rate, median lethal time (LT50), and lethal concentration (LC50 and LC99) were calculated. Also, for in vivo acaricidal activity of the same extracts were done using fifty intact New Zealand white male rabbits allocated to five experimental groups (n = 10). The first experimental group kept without infection as the negative control
Life
This study was conducted to investigate the effect of the Ficus sycomorus extract on Eimeria inte... more This study was conducted to investigate the effect of the Ficus sycomorus extract on Eimeria intestinalis in experimentally infected rabbits. For this purpose, forty male 30-day-old rabbits (Blanc de Bouscat) were divided into four groups (n = 10 in each group). Rabbits kept in the first group served as negative control (non-treated-non-infected). Rabbits kept in the second, third, and fourth groups were challenged at 10 weeks old with 3 × 104E. intestinalis sporulated oocysts. The third and fourth groups were treated orally with diclazuril 10% (0.05 mg/kg body weight) and F. sycomorus (100 mg/Kg) for three consecutive days, respectively. The efficacy was assessed based on the growth performance parameters, clinical symptoms, oocyst shedding, histopathological findings, and hematological parameters for 16 days post challenge. The study revealed that rabbits treated with F. sycomorus methanolic extract and diclazuril showed mild clinical symptoms with a significant decrease in oocyst...
Processes, 2021
In the present study, a phytochemical of Ficus sycomorus (Moraceae family) was screened, and the ... more In the present study, a phytochemical of Ficus sycomorus (Moraceae family) was screened, and the effect of this extract on rabbit performance indices, immunity, and carcass quality measures was determined. Ficus sycomorus samples were collected, air-dried, and extracted with 70% methanol to prepare a solution of 100 mg/mL concentration. The extract was subjected to high-resolution mass spectrometric measurements via ultra-high performance liquid chromatography-quadrupole time-of-flight-nanospray mass spectrometry (UPLC-QToF-MS) and 1H NMR analysis. Forty-eight male rabbits, one-month-old, belonging to the Blanc de Bouscat and New Zealand White breeds were selected and distributed equally in a 2 × 3 factorial trial. The rabbits within each breed received F. sycomorus extract at the dose of 0, 100, and 200 mg/kg for 60 days. Blood samples were collected and serum obtained for the detection of liver enzymes, serum lipids, and proteins. The results of UPLC-QToF-MS and molecular networki...
American Journal of Pharmacology and Toxicology, 2021
The study was conducted to determine the effect of bromocriptine and nano-bromocriptine on extern... more The study was conducted to determine the effect of bromocriptine and nano-bromocriptine on external and internal egg qualities of late laying hens. A total of 150 Novo-gene brown strain hens of 70 weeks of age were selected from a commercial laying farm. The birds were divided randomly into three groups, with 50 birds in each. The first group was given saline and kept as control, while the second and third groups were given bromocriptine (2-bromoalpha-ergocriptine) and nano-bromocriptine, respectively. Each group was separated into two subgroups of equal number, the first receiving the treatment orally and the second receiving the treatment via subcutaneous injections at a dose of 100 microg/kg body weight/ week. During experimentation, five eggs were taken every 4 weeks from each subgroup to determine the internal and the external egg qualities. Results revealed that Administration of either bromocriptine or nano-bromocriptine increased significantly the egg weight, egg length, yolk height, yolk weight, yolk width and thick albumin height. The findings conclude that bromocriptine and nano-bromocriptine treatment could be used for the late laying hens to improve the internal and external qualities of eggs.
Animals, 2021
The current study aimed to investigate the potential use of nano-bromocriptine in improving the l... more The current study aimed to investigate the potential use of nano-bromocriptine in improving the laying performance of late laying hens by modulating the prolactin gene expression. A total of 150 NOVOgen brown laying hens aged 70 weeks were randomly allocated into three groups of 50 birds each. The first group was kept as a control, while the second and the third groups were treated with bromocriptine and nano-bromocriptine, respectively, at a dose of 100 µg/kg body weight per week. The pause days, egg production, feed per dozen egg, and Haugh unit were determined on a monthly basis. Also, the relative prolactin gene expression in the pituitary gland was quantified using qPCR and the number of the ovarian follicles was determined after slaughtering at the 84th week of age. It was found that nano-bromocriptine and bromocriptine improved egg laying performance with minimal pause days, reduced feed per dozen egg, and depressed the relative prolactin gene expression; however, nano-bromoc...
American Journal of Animal and Veterinary Sciences, 2019
This study was conducted to evaluate the direct and the indirect standardized effects of some dai... more This study was conducted to evaluate the direct and the indirect standardized effects of some dairy performance parameters (days dry, lactation length, parity, total milk yield and 305-day milk yield) on the milk Somatic Cell Count (SCC) of Holstein cows throughout modeling of these variables with the path analysis technique. During studying 617 Holstein cows were enrolled in the experiment with the average Days in Milk (DIM) of 15±7.14 and still in the trial till the end of their lactation length. Milk samples were taken from each cow every three months; then, the fresh milk samples subjected to somatic cell counting via automatic cell counter. Other data of days dry, lactation length, parity, total and actual 305-day milk yields obtained from the dairy farm recording system (Dairycomp). After collection of all data, the data entered to AMOS software program version 24 to build a path model among days dry, lactation length, parity as independent variables and total, 305-day milk yields as intermediate transitional variables and milk SCC as a dependent variable. The study revealed that the SCC was directly affected by lactation length, total and 305-day milk yields, while it indirectly affected with total milk yield. Moreover, the overall standardized effect of the total milk yield on the milk SCC was week as a result of the negative direct effect-0.173 neutralized with another positive indirect one 0.229.
Animals, 2021
This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasm... more This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GFequina Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GFequina) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium th...
Journal of Clinical Oncology, Jun 1, 2023
Frontiers in Oncology
BackgroundOvarian cancer (OC) is the deadliest gynecological cancer, often diagnosed at advanced ... more BackgroundOvarian cancer (OC) is the deadliest gynecological cancer, often diagnosed at advanced stages. A fast and accurate diagnostic method for early-stage OC is needed. The tumor marker gangliosides, GD2 and GD3, exhibit properties that make them ideal potential diagnostic biomarkers, but they have never before been quantified in OC. We investigated the diagnostic utility of GD2 and GD3 for diagnosis of all subtypes and stages of OC.MethodsThis retrospective study evaluated GD2 and GD3 expression in biobanked tissue and serum samples from patients with invasive epithelial OC, healthy donors, non-malignant gynecological conditions, and other cancers. GD2 and GD3 levels were evaluated in tissue samples by immunohistochemistry (n=299) and in two cohorts of serum samples by quantitative ELISA. A discovery cohort (n=379) showed feasibility of GD2 and GD3 quantitative ELISA for diagnosing OC, and a subsequent model cohort (n=200) was used to train and cross-validate a diagnostic model...
Journal of Current Veterinary Research
This study aimed to investigate the effect of climatic factors, including temperature-humidity in... more This study aimed to investigate the effect of climatic factors, including temperature-humidity index (THI) and rainfall level (RFL) regarding the parity on the incidence of mastitis, milk yield, and composition in Holstein cattle in Egypt. A total of 2496 lactating cows were used from different four dairy farms located in Egypt. One hundred dairy cows were selected from each dairy herd for detection of the effect of different climatic factors and parity on milk composition and somatic cell count (SCC).The data grouped according to parity into 1, 2, and >2 parity groups. Also, the animals grouped according to THI into low (<70), medium (70-80), and high (>80) THI level and regrouped according to RFL into low (<2 mm), medium (2-5 mm), and high (>5 mm) rainfall per month. The obtained results revealed an increase in daily milk yield, milk protein%, fat %, and SCC with the increase of parity. Also, high THI significantly (p≤ 0.05) decreased daily milk yield (29.45 kg) and increased SCC (415 × 103 cell/mL). In high THI and low RFL climatic conditions, multiparous cows were prone to the occurrence of clinical mastitis compared to primiparous ones (p≤ 0.001); especially during mid and late lactation stages. In conclusion, high THI and low RFL impaired dairy performance and increased the frequency of clinical mastitis.
Zagazig Veterinary Journal
This study was constructed to investigate the differences between three breeds of rabbits [New Ze... more This study was constructed to investigate the differences between three breeds of rabbits [New Zealand White (NZW), Rex and Egyptian Gabali (Al-Gabali)] under heat stress regarding growth traits, hematological, biochemical and immunological parameters, oxidative stress biomarkers as well as metabolic hormones. Thirty rabbits of each breed (4 weeks of age) were subjected to heat stress (32 ± 1°C, 70-80% relative humidity) in the summer season at the period from mid-July to the end of August 2019. Blood samples were collected at 10 th week of age. The results revealed that body weights of NZW and Rex were higher by 205 and 145 g, respectively than those of Al-Gabali as well as average daily gains of NZW and Rex were higher by 4.42 and 3.40 g/day, respectively compared with Al-Gabali (P <0.05). Average daily feed intake of Rex was lower by 7.65 g/day than those of NZW and 2.89 g/day compared with Al-Gabali (P <0.05) and they had the lowest feed to gain ratios (3.58±0.097). Aspartate aminotransferase, urea and creatinine were significantly lowered in the plasma of Al-Gabali compared with NZW and Rex whereas, Al-Gabali recorded higher total proteins (5.90±0.048 g/dL), total globulins (2.50±0.054 g/dL), antibody titer against sheep red blood cells (6.38±0.111) and superoxide dismutase (7.42±0.163 IU/L) than NZW (5.66±0.069 g/dL, 2.21±0.084 g/dL, 4.90±0.068 and 6.80±0.053 IU/L, respectively) and Rex (5.74±0.072 g/dL 2.25±0.095 g/dL, 4.25±0.095 and 6.88±0.050 IU/L, respectively). Al-Gabali bunnies had higher immunoglobulin G, immunoglobulin A, catalase and glutathione peroxidase than NZW and Rex. However, Al-Gabali recorded the lowest insulin (15.64±0.20 ng/mL), growth hormone (45.80±0.48 ng/mL) and triiodothyronine (136.20±2.15 ng/mL). In conclusion, Al-Gabali rabbits were better adapted to heat stress compared with NZW and Rex, which was reflected in the improvement of health, immunity and oxidative stress indicators as well as lower plasma metabolic hormones, but they didn't have the genetic potential for growth traits.
Journal of Current Veterinary Research
This study aimed to evaluation of the in vitro and in vivo acaricidal activity of the Ficus sycom... more This study aimed to evaluation of the in vitro and in vivo acaricidal activity of the Ficus sycomorus (F. sycomorus) against fur mites in rabbits. Methanol 70% was used to extract F. sycomorus extracts from either the leaf or stem bark. The in vitro acaricidal activity of F. sycomorus extracts was tested using 2 ml of each F. sycomorus extract at 0.125, 0.25, 0.5, and 1 mg/ml concentrations in each experimental Petri dish containing ten Sarcoptesscabiei (S. scabiei) mites/dish. Negative control mites were given distilled water, whereas positive control mites were given selamectin. The stereomicroscopic examination was then performed 30 minutes after incubation and was repeated hourly until 7 hours after incubation. The mortality rate, median lethal time (LT50), and lethal concentration (LC50 and LC99) were calculated. Also, for in vivo acaricidal activity of the same extracts were done using fifty intact New Zealand white male rabbits allocated to five experimental groups (n = 10). The first experimental group kept without infection as the negative control
Life
This study was conducted to investigate the effect of the Ficus sycomorus extract on Eimeria inte... more This study was conducted to investigate the effect of the Ficus sycomorus extract on Eimeria intestinalis in experimentally infected rabbits. For this purpose, forty male 30-day-old rabbits (Blanc de Bouscat) were divided into four groups (n = 10 in each group). Rabbits kept in the first group served as negative control (non-treated-non-infected). Rabbits kept in the second, third, and fourth groups were challenged at 10 weeks old with 3 × 104E. intestinalis sporulated oocysts. The third and fourth groups were treated orally with diclazuril 10% (0.05 mg/kg body weight) and F. sycomorus (100 mg/Kg) for three consecutive days, respectively. The efficacy was assessed based on the growth performance parameters, clinical symptoms, oocyst shedding, histopathological findings, and hematological parameters for 16 days post challenge. The study revealed that rabbits treated with F. sycomorus methanolic extract and diclazuril showed mild clinical symptoms with a significant decrease in oocyst...
Processes, 2021
In the present study, a phytochemical of Ficus sycomorus (Moraceae family) was screened, and the ... more In the present study, a phytochemical of Ficus sycomorus (Moraceae family) was screened, and the effect of this extract on rabbit performance indices, immunity, and carcass quality measures was determined. Ficus sycomorus samples were collected, air-dried, and extracted with 70% methanol to prepare a solution of 100 mg/mL concentration. The extract was subjected to high-resolution mass spectrometric measurements via ultra-high performance liquid chromatography-quadrupole time-of-flight-nanospray mass spectrometry (UPLC-QToF-MS) and 1H NMR analysis. Forty-eight male rabbits, one-month-old, belonging to the Blanc de Bouscat and New Zealand White breeds were selected and distributed equally in a 2 × 3 factorial trial. The rabbits within each breed received F. sycomorus extract at the dose of 0, 100, and 200 mg/kg for 60 days. Blood samples were collected and serum obtained for the detection of liver enzymes, serum lipids, and proteins. The results of UPLC-QToF-MS and molecular networki...
American Journal of Pharmacology and Toxicology, 2021
The study was conducted to determine the effect of bromocriptine and nano-bromocriptine on extern... more The study was conducted to determine the effect of bromocriptine and nano-bromocriptine on external and internal egg qualities of late laying hens. A total of 150 Novo-gene brown strain hens of 70 weeks of age were selected from a commercial laying farm. The birds were divided randomly into three groups, with 50 birds in each. The first group was given saline and kept as control, while the second and third groups were given bromocriptine (2-bromoalpha-ergocriptine) and nano-bromocriptine, respectively. Each group was separated into two subgroups of equal number, the first receiving the treatment orally and the second receiving the treatment via subcutaneous injections at a dose of 100 microg/kg body weight/ week. During experimentation, five eggs were taken every 4 weeks from each subgroup to determine the internal and the external egg qualities. Results revealed that Administration of either bromocriptine or nano-bromocriptine increased significantly the egg weight, egg length, yolk height, yolk weight, yolk width and thick albumin height. The findings conclude that bromocriptine and nano-bromocriptine treatment could be used for the late laying hens to improve the internal and external qualities of eggs.
Animals, 2021
The current study aimed to investigate the potential use of nano-bromocriptine in improving the l... more The current study aimed to investigate the potential use of nano-bromocriptine in improving the laying performance of late laying hens by modulating the prolactin gene expression. A total of 150 NOVOgen brown laying hens aged 70 weeks were randomly allocated into three groups of 50 birds each. The first group was kept as a control, while the second and the third groups were treated with bromocriptine and nano-bromocriptine, respectively, at a dose of 100 µg/kg body weight per week. The pause days, egg production, feed per dozen egg, and Haugh unit were determined on a monthly basis. Also, the relative prolactin gene expression in the pituitary gland was quantified using qPCR and the number of the ovarian follicles was determined after slaughtering at the 84th week of age. It was found that nano-bromocriptine and bromocriptine improved egg laying performance with minimal pause days, reduced feed per dozen egg, and depressed the relative prolactin gene expression; however, nano-bromoc...
American Journal of Animal and Veterinary Sciences, 2019
This study was conducted to evaluate the direct and the indirect standardized effects of some dai... more This study was conducted to evaluate the direct and the indirect standardized effects of some dairy performance parameters (days dry, lactation length, parity, total milk yield and 305-day milk yield) on the milk Somatic Cell Count (SCC) of Holstein cows throughout modeling of these variables with the path analysis technique. During studying 617 Holstein cows were enrolled in the experiment with the average Days in Milk (DIM) of 15±7.14 and still in the trial till the end of their lactation length. Milk samples were taken from each cow every three months; then, the fresh milk samples subjected to somatic cell counting via automatic cell counter. Other data of days dry, lactation length, parity, total and actual 305-day milk yields obtained from the dairy farm recording system (Dairycomp). After collection of all data, the data entered to AMOS software program version 24 to build a path model among days dry, lactation length, parity as independent variables and total, 305-day milk yields as intermediate transitional variables and milk SCC as a dependent variable. The study revealed that the SCC was directly affected by lactation length, total and 305-day milk yields, while it indirectly affected with total milk yield. Moreover, the overall standardized effect of the total milk yield on the milk SCC was week as a result of the negative direct effect-0.173 neutralized with another positive indirect one 0.229.
Animals, 2021
This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasm... more This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GFequina Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GFequina) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium th...