Ahmed Said - Academia.edu (original) (raw)

Papers by Ahmed Said

Frontiers in pediatrics, Feb 29, 2024

Introduction: Fluid overload on Extracorporeal Membrane Oxygenation (ECMO) is associated with wor... more Introduction: Fluid overload on Extracorporeal Membrane Oxygenation (ECMO) is associated with worse outcomes. Previous in vitro studies have attempted to quantify oxygenator-related insensible losses, as failure to account for this fluid loss may lead to inaccurate fluid balance assessment and potentially harmful clinical management, such as unnecessary exposure to diuretics, slow continuous ultrafiltration (SCUF), or continuous kidney replacement therapy (CKRT). We performed a novel in vivo study to measure insensible fluid losses in pediatric ECMO patients. Methods: Pediatric ECMO patients were approached over eleven months in the pediatric and cardiac intensive care units. The water content of the oxygenator inflow sweep gas and exhaust gas were calculated by measuring the ambient temperature and relative humidity at frequent intervals and various sweep flow. Results and discussion: Nine subjects were enrolled, generating 431 data points. The cohort had a median age of 11 years IQR [0.83, 13], weight of 23.2 kg IQR [6.48, 44.28], and body surface area of 0.815 m 2 IQR [0.315, 1.3725]. Overall, the cohort had a median sweep of 2.5 L/min [0.9, 4], ECMO flow of 3.975 L/ m 2 /min [0.75, 4.51], and a set ECMO temperature of 37 degrees Celsius [36.6, 37.2]. The calculated net water loss per L/min of sweep was 75.93 ml/day, regardless of oxygenator size or patient weight. There was a significant difference in median documented vs. calculated fluid balance incorporating the insensible fluid loss, irrespective of oxygenator size (pediatric oxygenator: 7.001 ml/kg/day [-12.37, 28.59] vs. -6.11 ml/kg/day [-17.44, 13.01], respectively, p = 0.005 and adult oxygenator: 14.36 ml/kg/day [1.54, 25.77] and 9.204 ml/kg/ day [-1.28, 22.05], respectively, p = <0.001). We present this pilot study of measured oxygenator-associated insensible fluid losses on ECMO. Our results are consistent with prior in vitro methods and provide the basis for future studies evaluating the impact of incorporating these fluid losses into patients' daily fluid balance on patient management and outcomes.

Zenodo (CERN European Organization for Nuclear Research), Jun 7, 2017

The current study aimed to investigate the possible effect of Ceratonia siliqua (carob) pods and ... more The current study aimed to investigate the possible effect of Ceratonia siliqua (carob) pods and Ficus carica (fig) fruits ether extracts against lead toxicity on hematological, biochemical parameters, growth performance and mortality % in Nile Tilapia (Oreochromis niloticus). One hundred and forty fish were divided into 7 groups. The first group was left as a control and fed a control diet, while, the second and third groups were exposed to 1/2 96 h LC50 (77.5 mg/L) and 1/4 96 hLC50 (38.7 mg/L) of Lead (Pb), respectively and provided with a control diet. The fourth and fifth groups were exposed to 1/2 96 h LC50 and 1/4 96 h LC50 of Pb, respectively and fed diet with 600 mg carob extract/kg diet. The sixth and seventh group were exposed to 1/2 96 h LC50 and 1/4 96 h LC50 of Pb, respectively and provided with diet contain 600 mg fig extract/kg diet. The hematological, biochemical parameters and growth performance was significantly decreased after exposure to 1/2 96 h LC50 and 1/4 96 hLC50 of Pb for 30, 60 and 90 days, while plasma glucose and mortality % were increased significantly. There were no significant changes in RBCs, Hb and PCV of O. niloticus exposed to 1/2 96 h LC50 and 1/4 96 h LC50 of Pb that fed diet enriched with carob extract 600 mg/kg diet for 90 days. The fish exposed to 1/2 96 h LC50 and 1/4 96 h LC50 of Pb and fed diet enriched with 600 mg/kg diet carob or fig for 90 days revealed no significant changes in plasma albumin, total protein, glucose and growth parameters with a reduction of mortality %. The current study highlighted the importance of carob and fig ether extract in the protection of Nile tilapia against the lead acetate toxicity.

AIP Advances, 2022

Organic-inorganic perovskite solar cells (PSCs) have been emerging as one of the most promising p... more Organic-inorganic perovskite solar cells (PSCs) have been emerging as one of the most promising photovoltaic technologies. Surface morphology is considered as a key-parameter in energy alignment and plays a dominant role in specifying the device performance. The large grains and low roughness enhance the transport of charge carriers from perovskite layers to the transport layers, and this reflects on the delivered power conversion efficiency (PCE). Therefore, in this study, we investigated the influence of the fabrication parameters on the grain size and the morphology of perovskite layers, thereby the PCE of PSCs. These parameters included the anti-solvent nature (chlorobenzene or toluene), annealing temperature, and annealing ramp rate (slow or flash annealing). The scanning electron microscope confirmed that flash annealing results in a better morphology than slow annealing whether by employing an anti-solvent dripping or not. Furthermore, the dripping of chlorobenzene as an anti-solvent produced better morphology and large grains compared with toluene. Therefore, combining chlorobenzene with flash annealing induced the formation of large grains, full cover, and a uniform perovskite layer, which reflects on the performance of the fabricated PSCs. Finally, employing the aforementioned optimum preparation parameters to fabricate carbon-based PSCs resulted in decent PCE, current density short circuit (Jsc), open circuit voltage (Voc), and fill factor (FF) of 7%, 11.3 mA/cm 2 , 0.74 V, and 34%, respectively.

Revue Méditerranéenne des Télécommunications, Jul 24, 2017

Systematic Reviews in Pharmacy, 2020

The present study plan to assess the salivary pH at the commencing of prosthodontic or orthodonti... more The present study plan to assess the salivary pH at the commencing of prosthodontic or orthodontic treatment and after two weeks, six weeks , and fourteen weeks. It was hypothesized that the PH values between the starting time and these follow up intervals was not significant. 30 patients were included in this study who are allready attend to commence prosthodontic and fixed orthodontic treatment. 20 patients were stating to wear orthodontic appliance while the remaining patients comenced to be rehabilitated by prosthodontic treatment. All the subjects based on inclusion and exclusion criteria. Saliva specimen were got from all subjects, instantly previous to the insertion of aplince or denture (T0; baseline evaluation), after two weeks (T1), six weeks (T2), and fourteen weeks (T3) from the baseline assessment. The pH values were measured with the GC Saliva-Check Kit (GC Corp., Leuven, Belgium). Univariate (ONE WAY ANOVA) test was used, and differences within groups were inspected by using multiple comparison method (L.S.D.). The salivary samples at different time intervals present a signifigant difference of the PH value, between each succeeding time periods, and also from the base line time T0 In conclusion, when the results of the present study is displayed, the hypothesis is rejected due to a significant difference was observed in PH value between the baseline salivary PH and the succeeding follow up periods.

Sadhana-academy Proceedings in Engineering Sciences, Feb 1, 2015

The aim of the present paper is to study the unsteady magneto-hydrodynamic viscous Couette flow w... more The aim of the present paper is to study the unsteady magneto-hydrodynamic viscous Couette flow with heat transfer in a Darcy porous medium between two infinite parallel porous plates considering Hall effect, and temperature dependent physical properties under constant pressure gradient. The parallel plates are assumed to be porous and subjected to a uniform suction from above and injection from below while the fluid is flowing through a porous medium that is assumed to obey Darcy's law. A numerical solution for the governing nonlinear partial differential equations coupled with set of momentum equations and the energy equation including the viscous and Joule dissipations is adopted. The effect of the porosity of the medium, the Hall current and the temperature dependent viscosity and thermal conductivity on both the velocity and temperature distributions are investigated. It is found that the porosity number M has a marked effect on decreasing the velocity distribution (owing to a simultaneous increase in Darcy porous drag). Also the temperature T is decreased considerably with increasing porosity number. With increasing Hall current parameter m, the velocity component u (x-direction) is considerably increased, whereas velocity component w (z-direction) is reduced. Temperatures are decreased in the early stages of flow but effectively increased in the steady state with increasing m.

ACS Applied Materials & Interfaces, Nov 6, 2019

Journal of the Turkish Chemical Society, Section A: Chemistry, May 31, 2023

Unfortunately, many toxic solvents are used in chemistry laboratories and in the manufacturing of... more Unfortunately, many toxic solvents are used in chemistry laboratories and in the manufacturing of materials, which poses a serious risk to process safety, the natural environment, and human health. In this review, different tools for solvent-free organic reactions have been surveyed as an approach for green chemistry, where many of the solvents are known to upset our ecosystems so an enormous research effort has been exerted during the last decade to avoid the utilization of hazardous solvents and the number of publications on solvent-free reactions has increased nearly exponentially. Obviously, this reflects the great interest in solventless reactions. In our survey, we will highlight the solvent-free organic reaction as an approach for green chemistry to cover work published up to nearly 2022.

Journal of Pharmacology and Experimental Therapeutics, Mar 19, 2018

Life, Apr 26, 2022

Background: Due to the increased use of titanium dioxide nanoparticles (TiO 2 NPs), the risks of ... more Background: Due to the increased use of titanium dioxide nanoparticles (TiO 2 NPs), the risks of their reprotoxic effect arise. This study anticipated examining the potential protective effects of GEO (geranium essential oil) components screened via GC/MS analysis against the reprotoxic impacts of TiO 2 NPs on male rats. Methods: Thirty-two adult male rats were randomly assigned to four groups: control, GEO (75 mg/kg bwt/orally/day/60 days), TiO 2 NPs (100 ppm/rat/IP/day/60 days), and TiO 2 NPs + GEO. After 60 days, hormonal assay, semen appraisal, lipid peroxidation, antioxidant enzymes, testis and prostate morphometry, and the steroidogenesis-related genes' mRNA expressions were assessed. Results: The TEM and DLS results demonstrated that synthesized TiO 2 NPs are spherical with minimal aggregations polydispersed and varying in size from 50 to 100 nm. TiO 2 NPs IP injection-induced sperm abnormalities decreased the percent of motile sperms in the sperm count, reduced sex hormone levels, altered the testicular oxidant/antioxidant status and mRNA expression of steroid-related genes, and induced architectural alterations in testicular, epididymal, and prostate gland tissues. GEO significantly rescued the TiO 2 NPs-altered spermiogram, sex hormones, and antioxidant capacity, restored the tissue architectures, and enhanced steroidogenesis-related gene mRNA expression. Conclusions: These findings may significantly contribute to developing combinatorial treatments for infertility associated with various environmental and industrial xenobiotic exposures.

Al-Azhar International Medical Journal

Background: Uterine atony is the greatest typical etiology of primary postpartum hemorrhage (PPH)... more Background: Uterine atony is the greatest typical etiology of primary postpartum hemorrhage (PPH). The atonic PPH rates of developed nations are rising despite regular active control of the third stage of work. Primary PPH continues to be the greatest cause of maternal mortality in less developed nations. Aim and objectives: To assess the effectiveness of rectal misoprostol, oxytocin infusion, and oxytocin intravenous bolus and infusion in reducing blood loss during and after elective caesarean delivery.

The Egyptian Journal of Forensic Sciences and Applied Toxicology

Background: One of the most essential and commonly utilized nanoparticles is zinc oxide nanoparti... more Background: One of the most essential and commonly utilized nanoparticles is zinc oxide nanoparticles (ZnO-NPs). They are widely used in commercial items such as sunscreens and daily-care products, as well as in the food industry as a food additive and in food packaging because of their antibacterial and fungicidal properties. Aim: The study aimed to evaluate the subacute toxic effects of different doses of ZnO-NPs on the kidneys of adult male albino rats. Methods: Forty adult male albino rats were divided into four groups (10 rats per group); Group I served as the control (Negative control), Group II ZnO-NPs treated group (10mg/kg/day), Group III ZnO-NPs treated group (100mg/kg/day) and Group IV ZnO-NPs treated group (200mg/kg/day) for 28 days orally. The levels of serum urea, creatinine, uric acid, and zinc were estimated. Furthermore, oxidative stress markers in kidney tissue, including malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were estimated. Histopathological examination of the kidney tissues by light microscope was performed. Results: Oral ZnO-NPs induced a significant increase in serum creatinine, urea, uric acid, and zinc in a dose-dependent manner as the higher the dose the more significant toxicity. Zinc oxide nanoparticles induced a significant elevation of MDA and a significant decrease in the antioxidant enzymes SOD and GPx in kidney tissue also in a dose-dependent manner as toxicity is more evident in the high doses. Also, significant histopathological changes were detected in the kidney tissues. Conclusion: It can be concluded that subacute oral administration of ZnO-NPs induces nephrotoxic effects in a dose-dependent manner. The present study recommends that full attention must be given to evaluating the safety and toxicological issues of nanoparticles on the tissue, cells, and macromolecule of the human body.

Science

The performance of perovskite solar cells with inverted polarity (p-i-n) is still limited by reco... more The performance of perovskite solar cells with inverted polarity (p-i-n) is still limited by recombination at their electron extraction interface, which also lowers the power conversion efficiency (PCE) of p-i-n perovskite-silicon tandem solar cells. A MgF x interlayer with thickness of ~1 nanometer at the perovskite/C 60 interface favorably adjusts the surface energy of the perovskite layer through thermal evaporation, which facilitates efficient electron extraction and displaces C 60 from the perovskite surface to mitigate nonradiative recombination. These effects enable a champion open-circuit voltage of 1.92 volts, an improved fill factor of 80.7%, and an independently certified stabilized PCE of 29.3% for a monolithic perovskite-silicon tandem solar cell ~1 square centimeter in area. The tandem retained ~95% of its initial performance after damp-heat testing (85°C at 85% relative humidity) for >1000 hours.

Zagazig Veterinary Journal, 2017

Pathogens, 2021

Coxiella burnetii is the causative agent of Q fever. It can infect animals, humans, and birds, as... more Coxiella burnetii is the causative agent of Q fever. It can infect animals, humans, and birds, as well as ticks, and it has a worldwide geographical distribution. To better understand the epidemiology of C. burnetii in Somalia, ticks infesting camels were collected from five different regions, including Bari, Nugaal, Mudug, Sool, and Sanaag, between January and March 2018. Collected ticks were tested for C. burnetii and Coxiella-like endosymbiont DNA by using IS1111, icd, and Com1-target PCR assays. Moreover, sequencing of the 16S-rRNA was conducted. Molecular characterization and typing were done by adaA-gene analysis and plasmid-type identification. Further typing was carried out by 14-marker Multi-Locus Variable-Number Tandem Repeats (MLVA/VNTR) analysis. The investigated ticks (n = 237) were identified as Hyalomma spp. (n = 227, 95.8%), Amblyomma spp. (n = 8, 3.4%), and Ripicephalus spp. (n = 2, 0.8%), and 59.1% (140/237) of them were positive for Coxiella spp. While Sanger sequ...

Suez Canal Veterinary Medicine Journal. SCVMJ, Jun 30, 2019

The object of this study was to investigate the effects of oral administration of Isoprinosine (1... more The object of this study was to investigate the effects of oral administration of Isoprinosine (163.3mg/ kg) for 21 days, in rabbit before vaccination with (RHVD) vaccine on cellular and humoral immune response. Forty rabbits were divided into four equal groups. 1 st group was left without treatment and used as control, 2 nd group was treated with oral dose of isoprinosine (163.3 mg/kg) for 21 days, 3 rd group was vaccinated with rabbit haemorrhagic viral disease (RHVD) vaccine and the 4 th group was orally administered with isoprinosine (163.3 mg/kg) for 21 days then was vaccinated with (RHVD) vaccine. Results proved that vaccinated treated rabbits showed a significant improvement in phagocytic percent, phagocytic index, lysozyme activity, nitric oxide, serum total protein, serum albumin, gamma globulins in comparison with vaccinated nontreated rabbits. DNA destruction percent observed in liver and kidney tissue samples of treated, vaccinated and treated vaccinated groups. In conclusion, it appears that isoprinosine improves the cellular immune response in vaccinated rabbits and it has a genotoxic effect on hepatocytes of vaccinated rabbits.

Advances in Animal and Veterinary Sciences, 2021

HPLC is one of the greatest prevailing techniques in analytical chemistry with the capability for... more HPLC is one of the greatest prevailing techniques in analytical chemistry with the capability for separation, identification, and quantification of analytes exists in food of

S everal broad spectrum antibiotics were widely used for treatment and prophylaxsis of some dange... more S everal broad spectrum antibiotics were widely used for treatment and prophylaxsis of some dangerous bacterial infection in rabbits (El-Nawawy et al., 2006). Cefepime is one of fourth-generation parenteral cephalosporin antibiotic that is used for treatment of pneumonia, febrile neutropenia, urinary tract infections. Cefepime monotherapy gives both an excellent microbiological clearance and a good clinical response (El-Dars et al., 2019). Antimicrobial drug residues are detected by chemical, biological and immunological methods. The use of high performance liquid chromatography (HPLC) is an effective, accurate, rapid and sensitive technique for the analysis of cefepime residues in the tissues (Orti et al., 2000). Intensive uncontrolled use of antimicrobials drugs in the rabbit industry especially if the withdrawal times are neglected leads to harmful effects on human health (Tajick and Shohreh, 2006). The possible human hazards health problems related to antibiotic residues have been report-research Article Abstract | Drug Residues in meat have serious effects on human health (e.g., increase antimicrobial resistance, carcinogenicity, mutagenicity, and hypersensitivity) which urge the control of veterinary drug residues to ensure consumers' health. Thus, this work was designed to determine cefepime residues quantitatively in different tissues of the rabbits (liver, kidneys and muscles) and to determine the withdrawal time of cefepime following intra-muscular (I/M) dosing of the drug using high performance liquid chromatography (HPLC). Twenty four healthy male rabbit (growing white New Zealand) were used and randomly allocated into two equal groups, 12 rabbits/each; group 1 (negative control) was I/M injected with sterile normal saline and group 2 was I/M injected with cefepime hydrochloride (75 mg/kg BW) once daily for 5 successive days. Rabbits were sacrificed on 1 st , 3 rd , 7 th and 14 th day post antibiotic injection and liver, kidney and breast muscles samples were collected for detection of residue by HPLC. Results showed that days of experiment and tissue type has an effect on cefepime residual level as there is highly significant interaction (p =0.0001). The highest cefepime residual level was detected in kidney followed by liver while the lowest level was recorded in muscle samples. Cefepime remained within the detectable limit in the breast muscle (on the 3 rd day) and in the kidney and liver tissues (on the 7 th day) following the last I/M injection of the drug. While on the 14 th day after last dose, the cefepime residues were disappear in all tested tissues. Cefepime administration caused non-marked changes of tested biochemical indices (liver, and kidney function tests) of rabbit then reached to safe levels during the withdrawal period at other time points. Therefore, it is recommended to pay attention to the proper withdrawal time (14 day) before slaughtering of cefepime-treated rabbits to make sure that rabbits tissues are free from cefepime residues and become safe for consumers.

Molecules, 2021

Scaffolds hybridization is a well-known drug design strategy for antitumor agents. Herein, series... more Scaffolds hybridization is a well-known drug design strategy for antitumor agents. Herein, series of novel indolyl-pyrimidine hybrids were synthesized and evaluated in vitro and in vivo for their antitumor activity. The in vitro antiproliferative activity of all compounds was obtained against MCF-7, HepG2, and HCT-116 cancer cell lines, as well as against WI38 normal cells using the resazurin assay. Compounds 1–4 showed broad spectrum cytotoxic activity against all these cancer cell lines compared to normal cells. Compound 4g showed potent antiproliferative activity against these cell lines (IC50 = 5.1, 5.02, and 6.6 μM, respectively) comparable to the standard treatment (5-FU and erlotinib). In addition, the most promising group of compounds was further evaluated for their in vivo antitumor efficacy against EAC tumor bearing mice. Notably, compound 4g showed the most potent in vivo antitumor activity. The most active compounds were evaluated for their EGFR inhibitory (range 53–79%)...

Egyptian Journal of Aquatic Biology and Fisheries, 2021