Ahmet Var - Academia.edu (original) (raw)

Papers by Ahmet Var

Turkiye Klinikleri Journal of Case Reports, 2002

Purpose:Oxidative/antioxidative balance is changed in pa tients undergoing chronic hemodialysis. ... more Purpose:Oxidative/antioxidative balance is changed in pa tients undergoing chronic hemodialysis. The aim of this study was to evaluate the lipid peroxidation and antioxidative enzyme activities for different membranes in pa tients with chronic renal failure, before and after hemodi alysis. Materials and Methods: Serum MDA levels and erythrocyte GSH-Px, SOD activities were measured from 76 patients that underwent hemodialysis with different dialysing membranes (polysulfone, hemophane, cellulose). Statis tical analysis was performed by using Student's t test. Results:There was a statistically significant difference be tween the MDA levels determined before and after the hemodialysis, MDA levels decreased significantly after hemodialysis (p<0.005). Similarly, there was also an in crease in the GSH-Px (pO.001) and SOD (p<0,05) en zyme activities after hemodialysis when compared to the levels determined before hemodialysis. In hemodialysis with 3 different membrane type; hemodialysis with poly sulfone membrane induced significant changes in plasma MDA (p<0.005). Erythrocyte SOD and GSH-Px activi ties increased insignificantly in hemodialysis with 3 different membrane type. Conclusion: Our results suggest that use of polysulfone mem branes for hemodialysis prevents the formation of free radicals and consequently increases the antioxidant en zyme activities.

Turkish Thoracic Journal/Turk Toraks Dergisi, 2011

Kronik obstrüktif akciğer hastalığında (KOAH) hava yolu inflamatuar ve oksidatif stres belirteçle... more Kronik obstrüktif akciğer hastalığında (KOAH) hava yolu inflamatuar ve oksidatif stres belirteçlerinin arttığı ve bunun akciğer fonksiyonlarında daha hızlı düşme ile ilişkili olduğu düşünülmektedir. Oksidatif stresin sistemik belirteçleri arasında nitrik oksit (NO) ve malonil dealdehit (MDA), antioksidan belirteçler arasında da eritrosit superoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) yer almaktadır. Çalışmamızda KOAH'lı hastalardaki sistemik oksidan ve antioksidan belirteç düzeylerinin fonksiyonel parametrelerle ilişkisini araştırdık. Gereç ve Yöntem: KOAH tanılı ve bilinen sistemik inflamatuar ek hastalığı olmayan 51 hasta çalışmaya alındı. Tüm olguların solunum fonksiyon testleri değerlendirildi, 6 dakika yürüme testi (6DYT) ve dispne derecelendirilmesi (VAS) yapıldı. Çalışmaya 12 sağlıklı kontrol da dahil edildi. Hasta ve kontrol grubunda, venöz kanda eritrositlerde SOD, GSH-Px ve serumda NO, MDA düzeyleri bakıldı. Bulgular: Hastaların yaş ortalaması 62.94±10.56 yıl olarak bulundu. Kontrol ve hasta grubu arasında MDA (p<0.001), NO (p=0.012) ve GSH-Px (p=0.012) açısından anlamlı fark mevcuttu; her üç belirteç de kontrol grubunda KOAH grubuna göre yüksekti ancak yaş faktörüne göre düzeltme yapıldığında sadece MDA (p=0.017) kontrol grubunda daha yüksek saptandı. 1. saniyede zorlu ekspiratuvar volüm (FEV 1) düştükçe NO, SOD, MDA ve GSH-Px istatistiksel anlamlı olmasa da artmaktaydı. FEV 1 gibi 6DYT ve VAS ile de araştırılan parametrelerin hiçbirisi ile istatistiksel anlamlı ilişkisi yoktu. Sonuç: Çalışmamızda KOAH'ta oksidan ve antioksidan denge bozukluğunun fonksiyonel parametrelere yansımasını saptayamadık. Bu durum, lokal bulguların sistemik olarak tam yansımaması yanında KOAH'ta antioksidanların sağlıklı kişilerdekine benzer bir denge oluşturmaya çalışmasına bağlı olabilir.

Atherosclerosis, 1997

The relationship between glycosylated haemoglobin (HbAe), lipid peroxide (LPO), superoxide dismut... more The relationship between glycosylated haemoglobin (HbAe), lipid peroxide (LPO), superoxide dismutase (SOD), glutathione peroxidase (GPx) with and without retinopathy in diabetes mellitus was investigated. The study included 57 patients with diabetes mellitus type II and 40 healthy controls. The control group and the group of type 1 I diabetics subdivided to with and without rctinopathy and evaluated for the following parameters. The mean values of Hb AI, and plasma LPO in diabetic patients were significantly higher than those in the control subjects (p < 0.001). Erythmcyte SOD activity of diabetic patients was found significantly lower compared with non diabetics @ < 0.001). There was no difference in eqthrocyte GPx activity between the normals and the diabetics. We have observed that Hbhl, and plasma LPO levels were higher in diabetic individuals with retinopatby than those without (p < 0.001). We concluded that increased LPO and decreased antioxidant deface syitem in diabetes mellitus may induce the onset of diabetic microangiopatic complications. 30 LysopbospbatidyIcbc4Ine in oxidized LDL may be IovoIvcd in lipoprotein Iipsse down-regulation in human monocytc-derived maeropbsges

… J Gynecol Obst, 2005

ÖZET Amaç: Uterusu olmayan, menopozdaki, sağlıklı, sigara içmeyen kadınlarda östrojen tedavisinin... more ÖZET Amaç: Uterusu olmayan, menopozdaki, sağlıklı, sigara içmeyen kadınlarda östrojen tedavisinin kardiyovasküler risk faktörleri üzerine etkisini araştırmak. Gereç ve Yöntemler: 2003-2004 yılları arasında Celal Bayar Üniversitesi Kadın Doğum Anabilim Dalı menopoz polikliniğine ...

Acta Obstetricia et Gynecologica Scandinavica, 2009

To determine if oxidative stress and endothelial dysfunction exist at the same time in a young, n... more To determine if oxidative stress and endothelial dysfunction exist at the same time in a young, non-obese group of patients with polycystic ovary syndrome (PCOS). Cross-sectional study. Celal Bayar University, School of Medicine, Manisa, Turkey. Thirty-one young, non-obese patients with PCOS and 23 age- and body mass index-matched controls. Following clinical and biochemical diagnosis, malonyldialdehyde (MDA), superoxide dismutase (SOD), von Willebrand Factor (vWF), and nitric oxide (NO) levels of patients and controls were measured and compared. To find out oxidative stress and endothelial dysfunction parameters. MDA (0.12+/-0.03 vs 0.10+/-0.03, p=0.01) and SOD (8.0+/-0.7 vs 7.28+/-0.8, p=0.001) levels were significantly higher in PCOS group while there was no difference in vWF (527.2+/-280.1 vs 568.1+/-276.8, p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05) and NO levels (169.9+/-47.4 vs 168.9+/-80, p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05). When the results of the PCOS patients were divided into two subgroups in terms of insulin resistance (IR- and IR + ), the IR- subgroup had significantly higher MDA levels compared to the control (0.125+/-0.03 vs 0.101+/-0.03, p=0.03). Though IR+ group also had higher MDA than the control group, it did not reach to a significant level (0.117+/-0.05 vs 0.101+/-0.03, p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05). Both IR- and IR+ groups had significantly higher SOD levels compared with control group (7.99+/-0.7 vs 7.28+/-0.8, p=0.009 and 8.22+/-0.8 vs 7.28+/-0.8, p=0.03, respectively). vWF and NO levels were not different among these three groups (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05). Oxidative stress is prominent while endothelial dysfunction does not exist in young, non-obese patients with PCOS.

Phytotherapy research : PTR, Jan 29, 2015

Coronary heart disease because of atherosclerosis is still the most common cause of mortality. El... more Coronary heart disease because of atherosclerosis is still the most common cause of mortality. Elevated levels of low-density lipoprotein and total cholesterol are major risk factors for atherosclerotic cardiovascular disease. The aim of this study was to evaluate the effects of the olive leaf extract on serum lipid profile, early changes of atherosclerosis and endothelium-dependent relaxations in cholesterol-fed rats. For this purpose, rats were fed by 2% cholesterol-enriched or standard chow for 8 weeks. Some rats in each group were also fed orally by olive leaf extract at doses of 50 or 100 mg/kg/day. Atorvastatin at dose of 20 mg/kg of body weight daily was also given as positive control. After 8 weeks, lipid profiles of rat serums were analyzed. Antioxidant enzyme activities (superoxide dismutase and glutathione peroxidase) and degree of lipid peroxidation (malondialdehyde levels) were also measured in the hearts isolated from rats. In addition, expression of adhesion molecules...

Background and Design: Immunological factors are considered to have an important role in the mult... more Background and Design: Immunological factors are considered to have an important role in the multifactorial ethiopathogenesis of Behçet’s disease (BD). Especially, Th1 cells function by expressing proinflammatory cytokines and neutrophil-attracting chemokines. Levels of IL-2R, IL-6 and IL-8 were evaluated in patients with BD in this study. Material and Method: Sixty-six patients with BD (36 with inactive and 30 with active) composed the study group and 22 subjects – the control group. Cytokine analyses were done with ELISA method by using commercial kits. ESR and CRP levels were also measured.Results: IL-2R, IL-6 and IL-8 levels in all patients, including the inactive ones, were not different from those in controls. CRP and ESR levels in patients were higher than in controls. IL-8 and CRP levels were increased in patient with active BD (p<0.05) when compared to those with inactive BD. Conclusion: Our study demonstrated elevated levels of serum IL-8, a chemokine activating monunuc...

Journal of Cardiothoracic and Vascular Anesthesia, 2015

conditions. The last meta-analysis of randomized controlled trials (RCTs) in heart surgery did no... more conditions. The last meta-analysis of randomized controlled trials (RCTs) in heart surgery did not show any association between transfusion trigger and change in adverse events including mortality [1]. However, contemporary RCTs revealed contrary results. The aim of this study was to compare the longest follow-up mortality of restrictive and liberal blood transfusion strategies in adult cardiac surgery patients by meta-analysis of RCTs. Methods. Studies were identified in PubMed, The Web of Knowledge, Transfusion Evidence Library, and ClinicalTrials. gov databases from January 2000 to March 2015 by two researchers independently. Inclusion criteria were RCTs comparing liberal and restrictive transfusion triggers in adult cardiac surgery and evaluating mortality. Data were analysed according to the intention to treat. Mantel-Haenszel method with randomeffects analysis model was performed using RevMan 5 software. Results. Two conference proceedings were excluded due to the lack of the necessary information. Five trials randomizing 2,670 patients were included. The overall analysis showed that the use of liberal transfusion strategy was associated with a statistically significant reduction in the longest follow-up mortality compared with liberal strategy (40/1,339 [3.0%] in the liberal group versus 61/1,331 [4.6%] in the restrictive group; Odds Ratio, 1.55; 95% CI, 1.03À2.33; p ¼ 0.04). There was no heterogeneity between trials (p ¼ 0.49; I 2 ¼ 0%). Conclusion. The use of liberal blood transfusion strategy may reduce the longest follow-up mortality in cardiac surgery patients.

Hepato-gastroenterology

It has been shown that hepatic blood inflow occlusion impairs liver regeneration. Our aim in this... more It has been shown that hepatic blood inflow occlusion impairs liver regeneration. Our aim in this study was to investigate the effect of trimetazidine, known as an anti-ischemic and anti-oxidant agent, on liver regeneration after hepatic blood inflow occlusion. Sprague-Dawley rats were randomized into three groups. Rats in group 1 underwent 65% hepatectomy. Rats in group 2 and 3 were subjected to 15 minutes of hepatic blood inflow occlusion during 65% hepatectomy. Rats were treated with saline (in group 1 and 2) or trimetazidine (in group 3) 30 minutes before operation. Serum level of aspartate transaminase, wet to dry liver weight ratio, and liver injury score in light microscopy were studied for the evaluation of liver injury. Liver regeneration was evaluated by PCNA-labeling index (the percentage of hepatocytes staining for proliferating cell nuclear antigen), mitotic index (the percentage of mitotic hepatocytes), and liver regeneration rate (the percentage of initial liver weigh...

Archives of gynecology and obstetrics, 2003

Atherosis is accepted to underlie the pathogenesis of preeclampsia, therefore we aimed to determi... more Atherosis is accepted to underlie the pathogenesis of preeclampsia, therefore we aimed to determine malonyldialdehyde (MDA) levels as a marker of lipid peroxidation, and lipoprotein(a) (Lp(a)), apolipoprotein A-1 (Apo A-1) and apolipoprotein B (Apo B) levels as a marker of atherogenic profile in preeclamptic and normal pregnant women. Twenty preeclamptic and 20 gestational-age matched normal pregnant patients were enrolled in the study, mean gestational ages for the preeclamptic and the control group were 33.9+/-1.4 and 35.5+/-0.7 weeks, respectively. Blood was withdrawn from the patients soon after diagnosis, and from the controls at their routine prenatal visits. MDA levels was significantly higher in preeclamptic patients (P=0.0003), but no difference was observed in Apo A-1 and Apo B and Lp(a) levels between the 2 groups. We consider that higher MDA was due to oxidative stress seen in preeclampsia, and similar Apo A-1 and Apo B and Lp(a) levels were due to lack of systemic ather...

Surgery Today, 2010

To investigate the effect of tadalafil on anastomotic healing in an ischemic small intestine. Sta... more To investigate the effect of tadalafil on anastomotic healing in an ischemic small intestine. Standardized transection and anastomosis in the small intestine were performed in 48 male Sprague-Dawley rats divided into four equal groups (n = 12): group 1, normal anastomosis; group 2, ischemic anastomosis; group 3, normal anastomosis+tadalafil treatment; group 4, ischemic anastomosis+tadalafil treatment. Ischemia was established by ligating 2 cm of mesentery on either side of the anastomosis. Tadalafil was given to the rats once a day at dose of 5 mg/kg. The anastomotic bursting pressures and hydroxyproline concentrations were measured on postoperative day 4. A histopathological evaluation of the anastomoses was also performed. The bursting pressure and hydroxyproline concentration in group 2 were significantly lower than those in the other groups. There was no difference in the hydroxyproline concentration among groups 1, 3, and 4. While there was no difference between groups 3 and 4, the bursting pressures were significantly higher in groups 3 and 4 than in group 1. The histopathological evaluation revealed no significant differences in inflammatory cell infiltration, vascularization, or anastomotic collagen deposition among the groups. Tadalafil treatment improved the anastomotic bursting pressure and the hydroxyproline concentration in both normal and ischemic small intestine anastomosis.

Pediatric Surgery International, 2010

This study aimed to investigate the apoptotic mechanisms, oxidative stress, and mechanisms of eff... more This study aimed to investigate the apoptotic mechanisms, oxidative stress, and mechanisms of effect of antibiotics and ursodeoxycholic acid (UDCA) in total parenteral nutrition (TPN)-associated liver injury. Four groups of young rabbits were used in the study as follows: Group 1 (n: 7): TPN + Metronidazole (30 mg/kg IV) + Gentamicin (6 mg/kg IV); Group 2 (n: 7): TPN + UDCA (15 mg/kg per oral); Group 3 (n: 6): TPN only; and Group 4 (n: 7): Control group. After 10 days, the animals were killed and livers were removed. Hepatic apoptosis, apoptotic proteins, malondialdehyde (MDA) and myeloperoxidase (MPO) levels were studied in liver, and direct bilirubin values were assessed in the blood samples. Direct bilirubin increased with TPN, and antibiotic combination, as the most effective group, significantly lowered its levels (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01). MDA values also showed significant differences in comparisons between G1 and G3 (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) and G1-G4 (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01). An increased number of apoptotic cells was detected particularly in G2 and G3, whereas the lowest levels, other than in the control group, were found in G1. All TUNEL-positive cell number data were statistically significant except between G2 and G3(p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Caspase-3 and Bax immunoreactivities were greatest in G2. Significant differences were shown in caspase-3 immunoreactivity between the groups (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01), except between G1 and G3 (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05). All comparisons between the groups were significant for Bax (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01). In contrast, Bcl-2 immunoreactivity was moderate and highest in G1: comparisons between G1 and the other groups demonstrated statistically significant differences (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01). Fas-L immunoreactivity was greatest in G2, and all comparisons between the groups were statistically significant (p…

Pediatric Nephrology, 2004

We investigated the changes in renal excretion of calcium, sodium, and potassium in asthmatic chi... more We investigated the changes in renal excretion of calcium, sodium, and potassium in asthmatic children treated with inhaled budesonide, an inhaled glucocorticoid. Twenty-two asthmatic patients (7 female, 15 male, mean age 10.1€4.3 years) treated with 400-600 mg/day inhaled budesonide and 23 healthy children (6 female, 17 male, mean age 10.2€2.8 years) were enrolled in the study. The parameters recorded were serum sodium, potassium, calcium, phosphorus, alkaline phosphatase (ALP), type I collagen carboxyterminal telopeptide (ICTP), osteocalcin, intact parathyroid hormone (PTH) levels, first spot morning urine calcium/creatinine ratio, sodium/potassium ratio, and daily renal calcium excretion rate (UCa-ER). These parameters were measured in the control group and pre-and post-budesonide treatment in asthmatic children. Serum electrolytes, ALP, PTH, ICTP, and UCa-ER were in the normal ranges and were not significantly different between controls and asthmatic children. Serum levels of ICTP increased, while levels of osteocalcin decreased after budesonide therapy in the asthmatic group (P=0.001, P=0.005). UCa-ER was decreased after budesonide therapy in asthmatics (P=0.000). In conclusion, moderate doses of inhaled budesonide cause hypocalciuria and decreased bone turnover. These results may be attributed to a mechanism compensating for decreased absorption of calcium in the gut due to the topical effect of swallowed budesonide rather than the systemic effects of the drug. Increased bone metabolism and decreased turnover may have an important role in this compensatory mechanism.

Medical Principles and Practice, 2012

Objective: To determine the risk of an association with some genetic polymorphisms involved in ve... more Objective: To determine the risk of an association with some genetic polymorphisms involved in venous thromboembolism (VTE) gene variations (FVL, FV H1299R, FII G20210A, MTHFR C677T, MTHFR A1298C, PAI-1 4G/5G, β-ﬁbrinogen -455 G → A, FXIII Val34Leu and GpIIIa HPA-1a) in cancer patients. Subjects and Methods: Among 78 cancer patients, 28 who had proven first episode of VTE were selected as the patient group, with 50 control samples selected from age-, sex- and body mass index-matched healthy volunteers (healthy group). The differences in frequency of genetic polymorphisms were found to be statistically insignificant between these two groups. Results: Logistic regression analysis after adjustment for age, sex, smoking and hypertension showed no difference. The screened mutations of these genes were not significantly associated with VTE risk. Conclusion: There is no possible benefit from genetic screening tests regarding VTE in cancer patients.

Gynecologic and Obstetric Investigation, 2003

Endothelial dysfunction underlies the pathogenesis of preeclampsia, but its mechanism has not yet... more Endothelial dysfunction underlies the pathogenesis of preeclampsia, but its mechanism has not yet been completely understood. Elevated oxygen free radicals may partially explain the endothelial cell damage. In this study, we have aimed to measure homocysteine (Hcy) and nitric oxide (NO) levels as endothelial dysfunction markers in preeclamptic women. Nineteen preeclamptic (33.9 ± 1.4 weeks) and 15 gestational-age-matched normal pregnant women (35.5 ± 0.7 weeks) were included in the study. Mean NO level was significantly lower (p < 0.001) and mean Hcy level was significantly higher (p < 0.001) in the preeclamptic group. Elevated Hcy and oxygen free radical levels could decrease NO levels due to the reaction with each other and reduced NO may increase blood pressure and ischemia in preeclamptic patients. We have concluded that increased Hcy and oxygen free radical levels, and decreased NO levels are closely associated with preeclampsia-related endothelial dysfunction.

Cutaneous and Ocular Toxicology, 2011

Behçet&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;am... more Behçet&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s disease (BD) is an inflammatory vasculitis. Endogenous nitric oxide (NO), produced by endothelial cells, has pleiotropic effects such as vasodilatator, antiplatelet, antiproliferative. Reactive oxygen species (ROS) are produced at sites of endothelial inflammation. ROS target polyunsaturated lipids, which results in malondialdehyde (MDA) production. The aim was to investigate the oxidative stress in BD patients by measuring MDA and total antioxidant status (TAS) levels and to establish a possible relationship with respect to NO levels regarding disease activity. 55 BD patients (30 active/25 inactive) and 20 healthy volunteers were included in the study. Blood samples were drawn following an overnight fasting. TAS and MDA levels were determined spectrophotometrically. Serum nitrite (NO(2-)) and nitrate (NO(3-)) levels were measured to estimate NO production. Data were expressed as mean ± SD. TAS levels were significantly lower in BD patients than the controls (1.19 ± 0.34 vs. 3.29 ± 0.89 mmol/L). In the active BD group, MDA levels (0.36 ± 0.19 nmol/mL) were significantly higher than both the inactive BD group (0.25 ± 0.18 nmol/mL) and controls (0.18 ± 0.41 nmol/mL). NO levels were significantly lower in the active group compared to the inactive group (18.0 ± 2.80 vs. 19.40 ± 2.70 µmol/L). MDA levels correlated negatively with NO levels in the active group. Decreased NO levels mediated by increased oxidative stress significantly contribute to endothelial dysfunction observed in BD.

Current Therapeutic Research, 2004

Background: Sepsis remains a difficult problem for clinicians, with its systemic effects and high... more Background: Sepsis remains a difficult problem for clinicians, with its systemic effects and high morbidity and mortality rates. The roles of oxidative stress, endothelial dysfunction, and lipid peroxidation in sepsis-induced organ damage are being investigated.

Clinical Biochemistry, 2012

Existance of low grade persistent inflammation in obese children may increase the risk of metabol... more Existance of low grade persistent inflammation in obese children may increase the risk of metabolic and cardiovascular events. The aim was to determine whether glucose intolerance has an influence on inflammatory markers in obese adolescents. Designs and methods: 45 obese adolescents (mean BMI: 30.34 ± 5.42 kg/m 2) were grouped as normal or impaired glucose tolerance. IL-6 and CRP levels were analyzed by commercially available kits. Chitotriosidase activity was measured by a fluorescence method and neopterin levels were determined by ELISA. Data were expressed as mean ± SD. Results: IL-6 and CRP levels were similar in the two groups. Serum neopterin levels were not different between the groups. The chitotriosidase activity was significantly higher in the IGT group than NGT (124.33 ± 51.97 μmol/L/h vs 84.50 ± 53.99 μmol/L/h, p = 0.04). Conclusion: Serum chitotriosidase activity is increased in obese adolescents with impaired glucose tolerance.

Cell Biochemistry and Function, 2003

During some surgical interventions, temporary occlusion of the hepatic blood supply may cause isc... more During some surgical interventions, temporary occlusion of the hepatic blood supply may cause ischaemia-reperfusion (I/R) injury and hepatic dysfunction. In this study the protective effect of defibrotide (DEF) was evaluated in a rat model of liver I/R injury. Four groups of rats were subjected to the following protocols: saline infusion without ischaemia, DEF infusion without ischaemia, DEF infusion with hepatic I/R, and saline infusion with hepatic I/R. After a midline laporatomy, liver ischaemia was induced by 45 min of portal occlusion. DEF 175 mg/kg À1 was infused before ischaemia in 10 ml of saline. The same volume of saline was infused into the control animals. At the end of the 45-min reperfusion interval, the animals were sacrified. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzyme activities were determined in haemolysates, and malondialdehyde (MDA) level in the liver tissue was measured. Tissue MDA levels were significantly higher in the I/R plus saline group compared to the sham operation control groups (p < 0.01 and p < 0.05, respectively). Tissue MDA levels decreased in the DEF plus I/R group compared to the I/R plus saline group (p < 0.05), but DEF could not reduce tissue lipid peroxidation to the levels of the control sham operation groups. SOD and GSH-Px enzyme activities were significantly higher in DEF-treated animals than in the other groups (p < 0.05). These results suggest that DEF protects liver against I/R injury by increasing the antioxidant enzyme levels.