Ajay Abhyankar - Academia.edu (original) (raw)
Papers by Ajay Abhyankar
Viral Immunology, 2006
Dengue virus infections have undergone dramatic expansion in range, affecting several tropical an... more Dengue virus infections have undergone dramatic expansion in range, affecting several tropical and subtropical regions of the world. Dengue virus causes life-threatening complications characterized by dengue hemorrhagic fever and dengue shock syndrome. No standard validated test systems are available for serological diagnosis of dengue virus infection. This creates problems in the diagnosis and proper management of patients. Evaluation of a Defense Research and Development Establishment (DRDE) dengue virus dipstick dot-ELISA test, developed in house, vis-à-vis commercially available immunodiagnostic kits was carried out for detection of IgM antibodies. The DRDE dengue dipstick dot-ELISA was performed on the basis of the dot-ELISA principle. Commercial tests, namely, the Panbio Dengue IgM Capture ELISA system (Panbio, Sinnamon Park, Australia) and Pathozyme Dengue M (Omega Diagnostics, Alva, UK), were performed according to the manufacturers' protocols. Ninety-one serum samples collected from the states of Kerala and Delhi, India during August and November of 2004 were used in the present study. The overall agreement among all three tests was found to be only 72.16% for IgM antibodies. Correlations between the DRDE dipstick dot-ELISA and the Panbio kit, between the DRDE dipstick dot-ELISA and the Pathozyme Dengue M kit, and between the Panbio kit and the Pathozyme Dengue M kit were found to be 96, 93, and 94%, respectively. Although the Panbio kit is widely used in various laboratories in India, the DRDE dipstick dot-ELISA promises to be a useful kit because of its field applicability and comparable sensitivity.
Hybridoma, 2008
... VoI. 131, No. 4. October 1983 Printed in U. S. A. PRODUCTION AND CHARACTERIZATION OF MONOCLON... more ... VoI. 131, No. 4. October 1983 Printed in U. S. A. PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO HUMAN INTERLEUKIN 2: STRATEGY AND TACTICS' KENDALL A. SMITH,*' MARGARET F. FAVATA,* AND STEPHEN OROSZLAN+ ...
Virology Journal, 2006
Dengue virus infection has recently taken endemic proportion in India implicating all the four kn... more Dengue virus infection has recently taken endemic proportion in India implicating all the four known dengue serotypes. There was a major dengue outbreak in northern India including Delhi in October-December, 2003 and again in 2004. We have carried out a detailed investigation of the 2004 outbreak by Serosurveillance, RT-PCR, nested PCR, virus isolation and genotyping. We also report the molecular epidemiological investigation of these outbreaks.
Viral Immunology, 2006
Dengue virus infections have undergone dramatic expansion in range, affecting several tropical an... more Dengue virus infections have undergone dramatic expansion in range, affecting several tropical and subtropical regions of the world. Dengue virus causes life-threatening complications characterized by dengue hemorrhagic fever and dengue shock syndrome. No standard validated test systems are available for serological diagnosis of dengue virus infection. This creates problems in the diagnosis and proper management of patients. Evaluation of a Defense Research and Development Establishment (DRDE) dengue virus dipstick dot-ELISA test, developed in house, vis-à-vis commercially available immunodiagnostic kits was carried out for detection of IgM antibodies. The DRDE dengue dipstick dot-ELISA was performed on the basis of the dot-ELISA principle. Commercial tests, namely, the Panbio Dengue IgM Capture ELISA system (Panbio, Sinnamon Park, Australia) and Pathozyme Dengue M (Omega Diagnostics, Alva, UK), were performed according to the manufacturers' protocols. Ninety-one serum samples collected from the states of Kerala and Delhi, India during August and November of 2004 were used in the present study. The overall agreement among all three tests was found to be only 72.16% for IgM antibodies. Correlations between the DRDE dipstick dot-ELISA and the Panbio kit, between the DRDE dipstick dot-ELISA and the Pathozyme Dengue M kit, and between the Panbio kit and the Pathozyme Dengue M kit were found to be 96, 93, and 94%, respectively. Although the Panbio kit is widely used in various laboratories in India, the DRDE dipstick dot-ELISA promises to be a useful kit because of its field applicability and comparable sensitivity.
Hybridoma, 2008
... VoI. 131, No. 4. October 1983 Printed in U. S. A. PRODUCTION AND CHARACTERIZATION OF MONOCLON... more ... VoI. 131, No. 4. October 1983 Printed in U. S. A. PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO HUMAN INTERLEUKIN 2: STRATEGY AND TACTICS' KENDALL A. SMITH,*' MARGARET F. FAVATA,* AND STEPHEN OROSZLAN+ ...
Virology Journal, 2006
Dengue virus infection has recently taken endemic proportion in India implicating all the four kn... more Dengue virus infection has recently taken endemic proportion in India implicating all the four known dengue serotypes. There was a major dengue outbreak in northern India including Delhi in October-December, 2003 and again in 2004. We have carried out a detailed investigation of the 2004 outbreak by Serosurveillance, RT-PCR, nested PCR, virus isolation and genotyping. We also report the molecular epidemiological investigation of these outbreaks.