Ajda Ota - Academia.edu (original) (raw)
Papers by Ajda Ota
European Journal of Lipid Science and Technology, 2015
Protoplasma, Jan 2, 2015
Fullerenes (C60) are some of the most promising carbon nanomaterials to be used for medical appli... more Fullerenes (C60) are some of the most promising carbon nanomaterials to be used for medical applications as drug delivery agents. Computational and experimental studies have proposed their ability to enter cells by penetrating lipid bilayers. The aim of our study was to provide experimental evidence on whether pristine C60 in physiological media could penetrate cell membranes. The effect was tested on phospholipid vesicles (liposomes) composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and validated on isolated human red blood cells (RBCs). We incubated the liposomes in an aqueous suspension of C60 and dissolved the lipids and C60 together in chloroform and subsequently formatted the liposomes. By differential scanning calorimetry measurements, we assessed the effect of C60 on the phospholipid thermal profile. The latter was not affected after the incubation of liposomes in the C60 suspension; also, a shape transformation of RBCs did not occur. Differently, by dispersing both C...
International Journal of Membrane Science and Technology, 2015
Journal of Biotechnology, 2014
Chemistry and Physics of Lipids, 2011
Food Research International, 2015
European Journal of Lipid Science and Technology, 2015
Protoplasma, Jan 2, 2015
Fullerenes (C60) are some of the most promising carbon nanomaterials to be used for medical appli... more Fullerenes (C60) are some of the most promising carbon nanomaterials to be used for medical applications as drug delivery agents. Computational and experimental studies have proposed their ability to enter cells by penetrating lipid bilayers. The aim of our study was to provide experimental evidence on whether pristine C60 in physiological media could penetrate cell membranes. The effect was tested on phospholipid vesicles (liposomes) composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and validated on isolated human red blood cells (RBCs). We incubated the liposomes in an aqueous suspension of C60 and dissolved the lipids and C60 together in chloroform and subsequently formatted the liposomes. By differential scanning calorimetry measurements, we assessed the effect of C60 on the phospholipid thermal profile. The latter was not affected after the incubation of liposomes in the C60 suspension; also, a shape transformation of RBCs did not occur. Differently, by dispersing both C...
International Journal of Membrane Science and Technology, 2015
Journal of Biotechnology, 2014
Chemistry and Physics of Lipids, 2011
Food Research International, 2015