Akari Miller - Academia.edu (original) (raw)
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University of Ottawa | Université d'Ottawa
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The growth kinetics of wild-type and nef mutant viruses of human immunodeficiency virus type 1 we... more The growth kinetics of wild-type and nef mutant viruses of human immunodeficiency virus type 1 were comparatively analysed in several human CD4 M cell lines. Delayed replication of nef mutant virus was observed in all cell lines examined. To determine the stage in the virus replication cycle that is affected by Nef, a single-round replication assay was performed. Initially, the expression of marker genes in transfected cells was examined in order to study the role of Nef in the late phase of infection. The results obtained indicated that Nef is dispensable during the transcription to virion pro-0001-5657 # 1998 SGM CEEH K. Tokunaga and others K. Tokunaga and others
The growth kinetics of wild-type and nef mutant viruses of human immunodeficiency virus type 1 we... more The growth kinetics of wild-type and nef mutant viruses of human immunodeficiency virus type 1 were comparatively analysed in several human CD4 M cell lines. Delayed replication of nef mutant virus was observed in all cell lines examined. To determine the stage in the virus replication cycle that is affected by Nef, a single-round replication assay was performed. Initially, the expression of marker genes in transfected cells was examined in order to study the role of Nef in the late phase of infection. The results obtained indicated that Nef is dispensable during the transcription to virion pro-0001-5657 # 1998 SGM CEEH K. Tokunaga and others K. Tokunaga and others