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Virulence factors and its expression is very important in the pathology of coagulase negative sta... more Virulence factors and its expression is very important in the pathology of coagulase negative staphylococcal urinary tract infections. This study was carried out to determine the prevalence of virulence genes in Staphylococcus saprophyticus isolated from urine samples of patients diagnosed with urinary tract infections (UTIs) in government hospitals in Lagos State, Nigeria. A total of 88 S. saprophyticus clinical isolates were subjected to multiplex polymerase chain reaction procedure for the amplification of nine virulence genes (sdrl, ssp, uafA, dsdA, capD, aas, rot, sarA and agr). The results of the study showed that 43 (49%) of the 88 isolates tested, carried sarA gene closely followed by 26 (30%), 8 (9%), 6 (7%), 5 (6%), 3 (3%) and 1 (1%) of aas, ssp, dsdA, rot, agr, and capD genes respectively. Gene's uafA and sdrl were not detected in any of the isolates except the reference strain that harboured sdrl gene. The present results showed that S. saprophyticus is an important ...

Virulence factors and its expression is very important in the pathology of coagulase negative sta... more Virulence factors and its expression is very important in the pathology of coagulase negative staphylococcal urinary tract infections. This study was carried out to determine the prevalence of virulence genes in Staphylococcus saprophyticus isolated from urine samples of patients diagnosed with urinary tract infections (UTIs) in government hospitals in Lagos State, Nigeria. A total of 88 S. saprophyticus clinical isolates were subjected to multiplex polymerase chain reaction procedure for the amplification of nine virulence genes (sdrl, ssp, uafA , dsdA , capD, aas, rot, sarA and agr). The results of the study showed that 43 (49%) of the 88 isolates tested, carried sarA gene closely followed by 26 (30%), 8 (9%), 6 (7%), 5 (6%), 3 (3%) and 1 (1%) of aas, ssp, dsdA, rot, agr , and capD genes respectively. Gene's uafA and sdrl were not detected in any of the isolates except the reference strain that harboured sdrl gene. The present results showed that S. saprophyticus is an important cause of UTIs among women in this geographical location with the presence of some of these virulence genes that encoded virulence factors of this organism.

This study was carried out to determine the presence of penicillinase producing strains of Staphy... more This study was carried out to determine the presence of penicillinase producing strains of Staphylococcus aureus in the nose and ear of apparently healthy students of Bells University of Technology, Ota. 160 nasal and ear swab samples were randomly collected and screened for the presence of penicillinase producing strains of S. aureus using standard microbiological procedures. Of the 160 samples collected, 62 (38.8%) yielded growth of S. aureus. Male students harboured majority of the bacteria (26.9%) while 11.9% of S. aureus were found among samples collected from female students. 28 (17.5%) of the sixty-two S. aureus isolates were penicillinase positive, while 34 (21.3%) were non-penicillinase producing strains. Antibiotic sensitivity test of species of S. aureus showed highest sensitivity to gentamicin and chloramphenicol and moderate sensitivity to augmentin, amoxicillin and tetracycline, but resistant to cotrimoxazole, cloxacillin and erythromycin. The threatening prevalence of...

Nigerian Journal Of Microbiology, 2017

Virulence factors and its expression is very important in the pathology of coagulase negative sta... more Virulence factors and its expression is very important in the pathology of coagulase negative staphylococcal urinary tract infections. This study was carried out to determine the prevalence of virulence genes in Staphylococcus saprophyticus isolated from urine samples of patients diagnosed with urinary tract infections (UTIs) in government hospitals in Lagos State, Nigeria. A total of 88 S. saprophyticus clinical isolates were subjected to multiplex polymerase chain reaction procedure for the amplification of nine virulence genes (sdrl, ssp, uafA, dsdA, capD, aas, rot, sarA and agr). The results of the study showed that 43 (49%) of the 88 isolates tested, carried sarA gene closely followed by 26 (30%), 8 (9%), 6 (7%), 5 (6%), 3 (3%) and 1 (1%) of aas, ssp, dsdA, rot, agr, and capD genes respectively. Gene's uafA and sdrl were not detected in any of the isolates except the reference strain that harboured sdrl gene. The present results showed that S. saprophyticus is an important ...

Virulence factors and its expression is very important in the pathology of coagulase negative sta... more Virulence factors and its expression is very important in the pathology of coagulase negative staphylococcal urinary tract infections. This study was carried out to determine the prevalence of virulence genes in Staphylococcus saprophyticus isolated from urine samples of patients diagnosed with urinary tract infections (UTIs) in government hospitals in Lagos State, Nigeria. A total of 88 S. saprophyticus clinical isolates were subjected to multiplex polymerase chain reaction procedure for the amplification of nine virulence genes (sdrl, ssp, uafA , dsdA , capD, aas, rot, sarA and agr). The results of the study showed that 43 (49%) of the 88 isolates tested, carried sarA gene closely followed by 26 (30%), 8 (9%), 6 (7%), 5 (6%), 3 (3%) and 1 (1%) of aas, ssp, dsdA, rot, agr , and capD genes respectively. Gene's uafA and sdrl were not detected in any of the isolates except the reference strain that harboured sdrl gene. The present results showed that S. saprophyticus is an important cause of UTIs among women in this geographical location with the presence of some of these virulence genes that encoded virulence factors of this organism.

This study was carried out to determine the presence of penicillinase producing strains of Staphy... more This study was carried out to determine the presence of penicillinase producing strains of Staphylococcus aureus in the nose and ear of apparently healthy students of Bells University of Technology, Ota. 160 nasal and ear swab samples were randomly collected and screened for the presence of penicillinase producing strains of S. aureus using standard microbiological procedures. Of the 160 samples collected, 62 (38.8%) yielded growth of S. aureus. Male students harboured majority of the bacteria (26.9%) while 11.9% of S. aureus were found among samples collected from female students. 28 (17.5%) of the sixty-two S. aureus isolates were penicillinase positive, while 34 (21.3%) were non-penicillinase producing strains. Antibiotic sensitivity test of species of S. aureus showed highest sensitivity to gentamicin and chloramphenicol and moderate sensitivity to augmentin, amoxicillin and tetracycline, but resistant to cotrimoxazole, cloxacillin and erythromycin. The threatening prevalence of...

Nigerian Journal Of Microbiology, 2017