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Alberto Perez

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Papers by Alberto Perez

Circulation, 2002

Background-We sought to determine the degree to which B-type natriuretic peptide (BNP) adds to cl... more Background-We sought to determine the degree to which B-type natriuretic peptide (BNP) adds to clinical judgment in the diagnosis of congestive heart failure (CHF).

New England Journal of Medicine, 2002

The present study was undertaken to identify the congestive heart failure patients by BNP test. T... more The present study was undertaken to identify the congestive heart failure patients by BNP test. To measure BNP levels in Patients with congestive heart disease, to use the BNP in the heart failure treatment and Routine use of BNP assays may help to more rapidly identify patients with heart failure, thereby saving money on unnecessary echocardiograms. In the present study observe the measurement of B-type natriuretic peptide in the heart failure patients.

American Journal of Kidney Diseases, 2003

Plasma brain natriuretlc peptide (BNP) and norepinephrine (NE) are important predictors of heart ... more Plasma brain natriuretlc peptide (BNP) and norepinephrine (NE) are important predictors of heart failure (HF) morlallty and morbidity (M&M). Whether changes in BNP and NE over time correspond to subsequent changes in M&M has not been studied. Val-HeFT evaluated the efficacy of valsartan in HF patients and measured BNP and NE at baseline (BL) and during follow-up. Methods: BL and % change from BL to 4 months in BNP (n=3740) and NE (n=3746) were analysed by quartiles (0) for subsequent mortality and morbidity (death, sudden death with resuscitation. IV inotropic therapy, and hospitalisation for HF), usmg a Cox proportional hazard model with BL value as a covariate. The hazard risk ratio (RR) and 95% Cl for changes between Q, using first Q as control was calculated for mortality and first motiid event in all patients. irrespecttve of treatment.

Vibrio cholerae 638 (El Tor, Ogawa), a new CTX⌽-negative hemagglutinin/protease-defective strain ... more Vibrio cholerae 638 (El Tor, Ogawa), a new CTX⌽-negative hemagglutinin/protease-defective strain that is a cholera vaccine candidate, was examined for safety and immunogenicity in healthy adult volunteers. In a double-blind placebo-controlled study, no significant adverse reactions were observed in volunteers ingesting strain 638. Four volunteers of 42 who ingested strain 638 and 1 of 14 who received placebo experienced loose stools. The strain strongly colonized the human small bowel, as evidenced by its isolation from the stools of 37 of 42 volunteers. V. cholerae 638, at doses ranging from 4 ؋ 10 7 to 2 ؋ 10 9 vibrios, elicited significant serum vibriocidal antibody and anti-Ogawa immunoglobulin A antibody secreting cell responses.

International Endodontic Journal, 2003

To test the hypothesis that MG-63 osteosarcoma cells and primary osteoblasts react differently to... more To test the hypothesis that MG-63 osteosarcoma cells and primary osteoblasts react differently to ProRoot trade mark MTA (mineral trioxide aggregate) and White MTA by: (i) investigating the attachment of primary osteoblasts and MG-63 osteosarcoma cells to ProRoot trade mark MTA and White MTA; and (ii) comparing the osteogenic behaviour of both cell lines in contact with these endodontic materials. Primary osteoblasts were harvested from foetal rat calvaria by sequential digestion and MG-63 osteosarcoma cells were purchased. Cells were exposed to ProRoot trade mark MTA and White MTA prepared according to the manufacturer's instructions. All samples and controls were prepared in quadruplicate. After 6, 9 and 13 days exposure to MTA, the cells were fixed and prepared for SEM examination. In addition, both the cell types were grown to confluence and exposed to beta-glycerophosphate and dexamethasone to assess mineralized nodule formation as a function of osteogenic behaviour. The number of cells on the surface of the culture dish and on top of the materials increased in all samples throughout the 3 time periods, except for White MTA where no primary osteoblasts were visible on top of the material by the end of 13 days. After exposing cells to differentiation medium nodules were observed in cultures of primary osteoblasts, but not of MG-63 osteosarcoma cells. Under the conditions of this study, whilst primary osteoblasts initially bound to White MTA, they did not survive on the surface by the end of 13 days. Primary osteoblasts formed mineralized nodules when exposed to differentiation medium, whilst MG-63 cells did not form nodules. As MG-63 cells do not behave osteogenically by forming mineralized nodules, and primary osteoblasts are more sensitive than MG-63 osteosarcoma cells to White MTA in cell culture, primary osteoblasts are more appropriate than MG-63 cells for testing endodontic materials in cell culture.