Aldona Dembinska-Kiec - Academia.edu (original) (raw)

Uploads

Papers by Aldona Dembinska-Kiec

Research paper thumbnail of PP26: Modification of fibrinogen

Nuclear Medicine Communications

Research paper thumbnail of Placha W, Gil D, Dembinska-Kiec A, Laidler PThe effect of PPARgamma ligands on the proliferation and apoptosis of human melanoma cells. Melanoma Res 13: 447-456

Melanoma Research

The peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear receptor fami... more The peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear receptor family of ligand-activated transcription factors. PPARγ ligands have been shown to inhibit the growth of cells from different cancer lineages. This study was designed to evaluate the effects of PPARγ receptor activation in human melanoma cell lines. The effects of its expression and activation on cell proliferation and apoptosis in human melanoma cell lines (early stage cancer [WM35] and metastatic tumour [A375]) were investigated. Reverse transcription-polymerase chain reaction and Western blot analysis showed that both human melanoma cell lines expressed PPARγ mRNA and protein. Treatment of cells transfected with the luciferase gene ligated to PPAR response element constructed promoter showed that ciglitazone and prostaglandin J2 (PGJ2), selective ligands for PPARγ, increased the luciferase activity, proving the induction of the PPARγ reporter gene. Ciglitazone and PGJ2 inhibited melanoma ...

Research paper thumbnail of Different population of platelet derived microparticles from Healthy donors stimulate angiogenesis in vitro

Introduction: Platelet derived microparticles (PMPs) are qualitatively and quantitatively diverse... more Introduction: Platelet derived microparticles (PMPs) are qualitatively and quantitatively diverse, depending on the proaggregatory agent challenge. Shedding of procoagulant PMPs is mediated by integrin-related mechanism. However the role of coagulation activators in microparticles formation and their potential angiogenic activity is scarcely known. Aim of this study was to compare pro-angiogenic potential of PMPs obtained by different proaggregatory stimulation on endothelial cells and analysis of gene expression profile and capillary-like structure formation after endothelial cell exposure to different classes of PMPs. Methods: PMPs were obtained by stimulation with agonists: ADP, collagen and thrombin. Human umbilical vein endothelial cells (HUVEC) were treated with 30 ug/ml of PMPs. HUVEC proliferation and pro-angiogenetic gene expression profile (VCAM-1, CD36, AC133, eNOS and vWF) measured by the quantitative real-time PCR method were analyzed. For the angiogenic properties the ...

Research paper thumbnail of Glu298Asp polymorphism of endothelial nitric oxide synthase gene and arterial structure and function

Research paper thumbnail of Mo-P6:416 PPAR gamma polimorphism 12PRO>ALA as the possible determinant of impaired glucose tolerance

Atherosclerosis Supplements, 2006

Research paper thumbnail of Tu-P7:66 Angiogenesis eNOS-/- mice used as model of human metabolic syndrome

Atherosclerosis Supplements, 2006

Research paper thumbnail of We-P13:324 Hypertension differentiates the group of patients with metabolic syndrome

Atherosclerosis Supplements, 2006

We have already reported that 7-ketocholesterol (7KCHO), an oxysterol existing in human atheroscl... more We have already reported that 7-ketocholesterol (7KCHO), an oxysterol existing in human atherosclerotic plaque, might render atherosclerotic plaques unstable by inducing apoptosis of vascular smooth muscle cells (SMC). Many oxysterols have been also detected in the plasma of humans. They are transported into the circulation by mainly low-density lipoprotein (LDL). The amount of 7KCHO present in atherosclerotic lesions may be regulated by serum 7KCHO level (s-7KCHO), because the lipids contained in atherosclerotic lesions axe mostly derived from serum LDL. However, the significance of s-KCHO is not fully understood. In this study, we examined the clinical significance of s-7KCHO in patients with metabolic syndrome (MS) or acute myocardial infaxction (AMI).

Research paper thumbnail of We-P13:319 Leptin to adiponectin ratio as the novel atherogenesis risk indicator

Atherosclerosis Supplements, 2006

Research paper thumbnail of Ligands of the peroxisome proliferator-activated receptors (PPAR-gamma and PPAR-alpha) reduce myocardial infarct size

The FASEB Journal, 2002

This study was designed to investigate the effects of various chemically distinct activators of P... more This study was designed to investigate the effects of various chemically distinct activators of PPAR-γ and PPAR-α in a rat model of acute myocardial infarction. Using Northern blot analysis and RT-PCR in samples of rat heart, we document the expression of the mRNA for ...

Research paper thumbnail of Vascular endothelial growth factor in monitoring therapy of hepatic haemangioendothelioma

Medical and Pediatric Oncology, 2003

Research paper thumbnail of VEGF–nitric oxide reciprocal regulation

Research paper thumbnail of Insulin Resistance and Short-Term Transdermal Hormone Replacement Therapy in Hypertensive Postmenopausal Women

Journal of Hypertension, 2000

Research paper thumbnail of Effect of 5-aminosalicylic acid and 4-aminosalicylic acid on precontracted rat aortic strips and on NO-mediated inhibition of platelet aggregation

Agents and Actions, 1994

ABSTRACT We have examined the interactions of 5-aminosalicylic acid (5-ASA) and 4-aminosalicylic ... more ABSTRACT We have examined the interactions of 5-aminosalicylic acid (5-ASA) and 4-aminosalicylic acid (4-ASA) with nitric oxide (NO) on rat aorta and human platelets. Phennylephrine-precontracted rat aortic strips with intact endothelium were further contracted by 5-ASA (50–200 mol/1) and 4-ASA (1–20 mmol/1) in a concentration-dependent manner. Removal of endothelium, inhibition of guanylate cyclase by methylene blue, inhibition of NO biosynthesis byN G-nitro-l-arginine as well as inactivation of NO by oxyhemoglobin abolished the effects of 5-ASA and 4-ASA. The antiaggregatory effects of 3-morpholinosydnonimine (SIN-1) and rat peritoneal neutrophils (RPN) were diminished in a concentration-dependent manner by 5-ASA (50–250 mol/l), but not by 4-ASA (up to 20 mmol/l). In the presence of superoxide dismutase (SOD), 5-ASA did not antagonize NO-mediated effects on platelets. 5-ASA up to 100 mol/l did not affect NO synthase from rat brain, while a concentration of 1 mM caused 21% inhibition. Since NO might act as a cytotoxic mediator, our results suggest that inactivation of NO by 5-ASA and higher concentrations of 4-ASA could contribute to the therapeutic activity of the drugs in inflammatory bowel disease (IBD).

Research paper thumbnail of Different response to fatty acids in metabolism and gene expression of human endothelial cells (HUVEC) and preadipocytes (SVF)

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012

Research paper thumbnail of Peroxisome proliferator-activated receptors (PPARS): regulators of gene expression in heart and skeletal muscle

Acta Physiologica Scandinavica, 2003

Research paper thumbnail of The Role of Dna Methylation in Pro-Chemotactic Influence of Beta-Carotene and Fatty Acids on Endothelial Cells

Atherosclerosis Supplements, 2008

Research paper thumbnail of DNA methylation, induced by beta-carotene and arachidonic acid, plays a regulatory role in the pro-angiogenic VEGF-receptor (KDR) gene expression in endothelial cells

Journal of physiology and pharmacology: an official journal of the Polish Physiological Society

DNA methylation is a potent regulator of gene expression. The influence of beta-carotene (BC) and... more DNA methylation is a potent regulator of gene expression. The influence of beta-carotene (BC) and arachidonic acid (AA) on angiogenesis--a new blood vessel formation, was reported. The tyrosine kinase VEGFR-2 receptor (KDR) activation by vascular endothelial growth factor is one of the main angiogenic mechanisms. This study was aimed to investigate a possible role of CpG island methylation on regulation of the pro-angiogenic KDR gene expression after incubation of human endothelial cells with BC and/or AA. METHODS: Human umbilical vein endothelial cells (HUVEC) were incubated with BC (1-10 microM) and/or 3 microM AA for 24 hours. The CpG island methylation was quantified using the COBRA method and restriction enzymes' digestion (NewEngland BioLabs). Intracellular protein concentrations were determined by Western blot analysis using the specific antibodies (Santa Cruz). Results: Incubation with BC and AA, decreased methylation of the KDR promoter region. These results well-correl...

Research paper thumbnail of Genetic variation in the TNF-alpha (NCO-1) and stress protein HSP70 (HSP70-2; HSP-HOM) gene is associated

Atherosclerosis Supplements

Research paper thumbnail of Insertion/deletion polymorphism of the angiotensin converting enzyme gene in relation to arterial stiffness in the population-based family study

American Journal of Hypertension, 2003

Objective: To evaluate an influence of the angiotensine converting enzyme (ACE) gene insertion/de... more Objective: To evaluate an influence of the angiotensine converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism on 24-hour ambulatory blood pressure (ABPM) and pulse wave velocity (PWV) in the cohort of Polish families.Methods: We genotyped 142 parents and 184 offspring, belonging to 86 nuclear families, enrolled in the population-based study in Krakow, Poland, for the I/D polymorphism of the ACE gene.

Research paper thumbnail of Differential effect of hormone replacement therapy on nitric oxide leves in hypertensive postmenopausal women

American Journal of Hypertension, 2001

Elevated plasma homocystine levels (HOM) have been proposed as an independent cardiovascular risk... more Elevated plasma homocystine levels (HOM) have been proposed as an independent cardiovascular risk factor. The effects of antihypertensive therapy on HOM are not known to date. To this end, we studied 150 consecutive patients with uncomplicated essential hypertension, who normalized their blood pressure (BP) with Candesartan 16 mg daily. HOM was measured at baseline and 6 months after Candesartan monotherapy. Overall, HOM was decreased from 12.9 to 12.1 g/L (-8.7% pϭ0.00004). HOM was decreased more in elderly patients (-12.8% vs -5.3% pϭ0.03), in patients with left ventricular hypertrophy (LVH, -12.8% vs -4.1% pϭ0.01) and in diabetics (-25.4% vs -6.7% pϭ0.008). HOM decrease magnitude was independent of systolic BP fall (rϭ0.01 pϭNS) and plasma renin activity (rϭ0.07 pϭNS), while it was related to diastolic BP fall (rϭ-0.24 pϭ0.003), diabetics status (rϭ-0.27 pϭ0.001), LV mass index baseline values (rϭ-0.31 pϽ0.0001) and LVH regression (rϭ0.18 pϭ0.03). It is concluded that Candesartan decreases HOM independently of BP normalization, especially in elderly, diabetic patients with LVH.

Research paper thumbnail of PP26: Modification of fibrinogen

Nuclear Medicine Communications

Research paper thumbnail of Placha W, Gil D, Dembinska-Kiec A, Laidler PThe effect of PPARgamma ligands on the proliferation and apoptosis of human melanoma cells. Melanoma Res 13: 447-456

Melanoma Research

The peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear receptor fami... more The peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear receptor family of ligand-activated transcription factors. PPARγ ligands have been shown to inhibit the growth of cells from different cancer lineages. This study was designed to evaluate the effects of PPARγ receptor activation in human melanoma cell lines. The effects of its expression and activation on cell proliferation and apoptosis in human melanoma cell lines (early stage cancer [WM35] and metastatic tumour [A375]) were investigated. Reverse transcription-polymerase chain reaction and Western blot analysis showed that both human melanoma cell lines expressed PPARγ mRNA and protein. Treatment of cells transfected with the luciferase gene ligated to PPAR response element constructed promoter showed that ciglitazone and prostaglandin J2 (PGJ2), selective ligands for PPARγ, increased the luciferase activity, proving the induction of the PPARγ reporter gene. Ciglitazone and PGJ2 inhibited melanoma ...

Research paper thumbnail of Different population of platelet derived microparticles from Healthy donors stimulate angiogenesis in vitro

Introduction: Platelet derived microparticles (PMPs) are qualitatively and quantitatively diverse... more Introduction: Platelet derived microparticles (PMPs) are qualitatively and quantitatively diverse, depending on the proaggregatory agent challenge. Shedding of procoagulant PMPs is mediated by integrin-related mechanism. However the role of coagulation activators in microparticles formation and their potential angiogenic activity is scarcely known. Aim of this study was to compare pro-angiogenic potential of PMPs obtained by different proaggregatory stimulation on endothelial cells and analysis of gene expression profile and capillary-like structure formation after endothelial cell exposure to different classes of PMPs. Methods: PMPs were obtained by stimulation with agonists: ADP, collagen and thrombin. Human umbilical vein endothelial cells (HUVEC) were treated with 30 ug/ml of PMPs. HUVEC proliferation and pro-angiogenetic gene expression profile (VCAM-1, CD36, AC133, eNOS and vWF) measured by the quantitative real-time PCR method were analyzed. For the angiogenic properties the ...

Research paper thumbnail of Glu298Asp polymorphism of endothelial nitric oxide synthase gene and arterial structure and function

Research paper thumbnail of Mo-P6:416 PPAR gamma polimorphism 12PRO>ALA as the possible determinant of impaired glucose tolerance

Atherosclerosis Supplements, 2006

Research paper thumbnail of Tu-P7:66 Angiogenesis eNOS-/- mice used as model of human metabolic syndrome

Atherosclerosis Supplements, 2006

Research paper thumbnail of We-P13:324 Hypertension differentiates the group of patients with metabolic syndrome

Atherosclerosis Supplements, 2006

We have already reported that 7-ketocholesterol (7KCHO), an oxysterol existing in human atheroscl... more We have already reported that 7-ketocholesterol (7KCHO), an oxysterol existing in human atherosclerotic plaque, might render atherosclerotic plaques unstable by inducing apoptosis of vascular smooth muscle cells (SMC). Many oxysterols have been also detected in the plasma of humans. They are transported into the circulation by mainly low-density lipoprotein (LDL). The amount of 7KCHO present in atherosclerotic lesions may be regulated by serum 7KCHO level (s-7KCHO), because the lipids contained in atherosclerotic lesions axe mostly derived from serum LDL. However, the significance of s-KCHO is not fully understood. In this study, we examined the clinical significance of s-7KCHO in patients with metabolic syndrome (MS) or acute myocardial infaxction (AMI).

Research paper thumbnail of We-P13:319 Leptin to adiponectin ratio as the novel atherogenesis risk indicator

Atherosclerosis Supplements, 2006

Research paper thumbnail of Ligands of the peroxisome proliferator-activated receptors (PPAR-gamma and PPAR-alpha) reduce myocardial infarct size

The FASEB Journal, 2002

This study was designed to investigate the effects of various chemically distinct activators of P... more This study was designed to investigate the effects of various chemically distinct activators of PPAR-γ and PPAR-α in a rat model of acute myocardial infarction. Using Northern blot analysis and RT-PCR in samples of rat heart, we document the expression of the mRNA for ...

Research paper thumbnail of Vascular endothelial growth factor in monitoring therapy of hepatic haemangioendothelioma

Medical and Pediatric Oncology, 2003

Research paper thumbnail of VEGF–nitric oxide reciprocal regulation

Research paper thumbnail of Insulin Resistance and Short-Term Transdermal Hormone Replacement Therapy in Hypertensive Postmenopausal Women

Journal of Hypertension, 2000

Research paper thumbnail of Effect of 5-aminosalicylic acid and 4-aminosalicylic acid on precontracted rat aortic strips and on NO-mediated inhibition of platelet aggregation

Agents and Actions, 1994

ABSTRACT We have examined the interactions of 5-aminosalicylic acid (5-ASA) and 4-aminosalicylic ... more ABSTRACT We have examined the interactions of 5-aminosalicylic acid (5-ASA) and 4-aminosalicylic acid (4-ASA) with nitric oxide (NO) on rat aorta and human platelets. Phennylephrine-precontracted rat aortic strips with intact endothelium were further contracted by 5-ASA (50–200 mol/1) and 4-ASA (1–20 mmol/1) in a concentration-dependent manner. Removal of endothelium, inhibition of guanylate cyclase by methylene blue, inhibition of NO biosynthesis byN G-nitro-l-arginine as well as inactivation of NO by oxyhemoglobin abolished the effects of 5-ASA and 4-ASA. The antiaggregatory effects of 3-morpholinosydnonimine (SIN-1) and rat peritoneal neutrophils (RPN) were diminished in a concentration-dependent manner by 5-ASA (50–250 mol/l), but not by 4-ASA (up to 20 mmol/l). In the presence of superoxide dismutase (SOD), 5-ASA did not antagonize NO-mediated effects on platelets. 5-ASA up to 100 mol/l did not affect NO synthase from rat brain, while a concentration of 1 mM caused 21% inhibition. Since NO might act as a cytotoxic mediator, our results suggest that inactivation of NO by 5-ASA and higher concentrations of 4-ASA could contribute to the therapeutic activity of the drugs in inflammatory bowel disease (IBD).

Research paper thumbnail of Different response to fatty acids in metabolism and gene expression of human endothelial cells (HUVEC) and preadipocytes (SVF)

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012

Research paper thumbnail of Peroxisome proliferator-activated receptors (PPARS): regulators of gene expression in heart and skeletal muscle

Acta Physiologica Scandinavica, 2003

Research paper thumbnail of The Role of Dna Methylation in Pro-Chemotactic Influence of Beta-Carotene and Fatty Acids on Endothelial Cells

Atherosclerosis Supplements, 2008

Research paper thumbnail of DNA methylation, induced by beta-carotene and arachidonic acid, plays a regulatory role in the pro-angiogenic VEGF-receptor (KDR) gene expression in endothelial cells

Journal of physiology and pharmacology: an official journal of the Polish Physiological Society

DNA methylation is a potent regulator of gene expression. The influence of beta-carotene (BC) and... more DNA methylation is a potent regulator of gene expression. The influence of beta-carotene (BC) and arachidonic acid (AA) on angiogenesis--a new blood vessel formation, was reported. The tyrosine kinase VEGFR-2 receptor (KDR) activation by vascular endothelial growth factor is one of the main angiogenic mechanisms. This study was aimed to investigate a possible role of CpG island methylation on regulation of the pro-angiogenic KDR gene expression after incubation of human endothelial cells with BC and/or AA. METHODS: Human umbilical vein endothelial cells (HUVEC) were incubated with BC (1-10 microM) and/or 3 microM AA for 24 hours. The CpG island methylation was quantified using the COBRA method and restriction enzymes' digestion (NewEngland BioLabs). Intracellular protein concentrations were determined by Western blot analysis using the specific antibodies (Santa Cruz). Results: Incubation with BC and AA, decreased methylation of the KDR promoter region. These results well-correl...

Research paper thumbnail of Genetic variation in the TNF-alpha (NCO-1) and stress protein HSP70 (HSP70-2; HSP-HOM) gene is associated

Atherosclerosis Supplements

Research paper thumbnail of Insertion/deletion polymorphism of the angiotensin converting enzyme gene in relation to arterial stiffness in the population-based family study

American Journal of Hypertension, 2003

Objective: To evaluate an influence of the angiotensine converting enzyme (ACE) gene insertion/de... more Objective: To evaluate an influence of the angiotensine converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism on 24-hour ambulatory blood pressure (ABPM) and pulse wave velocity (PWV) in the cohort of Polish families.Methods: We genotyped 142 parents and 184 offspring, belonging to 86 nuclear families, enrolled in the population-based study in Krakow, Poland, for the I/D polymorphism of the ACE gene.

Research paper thumbnail of Differential effect of hormone replacement therapy on nitric oxide leves in hypertensive postmenopausal women

American Journal of Hypertension, 2001

Elevated plasma homocystine levels (HOM) have been proposed as an independent cardiovascular risk... more Elevated plasma homocystine levels (HOM) have been proposed as an independent cardiovascular risk factor. The effects of antihypertensive therapy on HOM are not known to date. To this end, we studied 150 consecutive patients with uncomplicated essential hypertension, who normalized their blood pressure (BP) with Candesartan 16 mg daily. HOM was measured at baseline and 6 months after Candesartan monotherapy. Overall, HOM was decreased from 12.9 to 12.1 g/L (-8.7% pϭ0.00004). HOM was decreased more in elderly patients (-12.8% vs -5.3% pϭ0.03), in patients with left ventricular hypertrophy (LVH, -12.8% vs -4.1% pϭ0.01) and in diabetics (-25.4% vs -6.7% pϭ0.008). HOM decrease magnitude was independent of systolic BP fall (rϭ0.01 pϭNS) and plasma renin activity (rϭ0.07 pϭNS), while it was related to diastolic BP fall (rϭ-0.24 pϭ0.003), diabetics status (rϭ-0.27 pϭ0.001), LV mass index baseline values (rϭ-0.31 pϽ0.0001) and LVH regression (rϭ0.18 pϭ0.03). It is concluded that Candesartan decreases HOM independently of BP normalization, especially in elderly, diabetic patients with LVH.