Alejandro Blanco-labra - Academia.edu (original) (raw)

Papers by Alejandro Blanco-labra

Research paper thumbnail of Vicilin Storage Proteins from <i>Vigna unguiculata </i>(Legume) Seeds Inhibit Fungal Growth

Journal of Agricultural and Food Chemistry, Oct 1, 1997

ABSTRACT Vicilin storage proteins (7S globulins) isolated from Vigna unguiculata (cowpea) seeds w... more ABSTRACT Vicilin storage proteins (7S globulins) isolated from Vigna unguiculata (cowpea) seeds were shown to interfere with the germination of spores or conidia of the fungi Fusarium solani, Fusarium axysporum, Colletotrichum musae, Phytophtora capsici, Neurospora crassa, and Ustilago maydis sporidia. Cowpea vicilins have been shown to bind to fungal structures, possibly chitin-containing structures of the cell wall, and can be desorbed by strong acid. The results presented in this paper are in agreement with data previously obtained on the chitin-binding properties of cowpea vicilins and the effect they exert on the development and survival of the storage pest insect Callosobruchus maculatus (Coleoptera: Bruchidae).

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Research paper thumbnail of Purification and Partial Characterization of a Proteinase Inhibitor from Tepary Bean (Phaseolus Acutifolius) Seeds

Journal of Food Biochemistry, Oct 1, 1997

hi a qitnlitntive screeriing of 36 arcessions of tepary beam seeds, al1 tlie accessions inliibit ... more hi a qitnlitntive screeriing of 36 arcessions of tepary beam seeds, al1 tlie accessions inliibit tlte activity of bovirie trypsin and trypsin-like proteinases from the insect P. truncatus, arid tlie rnajorip of tliem inhibir a-amylase activity of severa1 importarit hect pests. A protein proteirrci-. inhibitor was purified from accessiori L-242-45, usirtg fracriorial precipitation, gelfiltration, ion encltange cfrromatography artd reverse-pltase HPLC. ilre protein showed un apparent molecular weigltt of 7,lW by PACE. However, corrtrary to other inhibitors previously reported, tlte inliibitory activity was only presenr iri tlre trimeric form. 7he protein was clraracterized as a serine-proteinase inliibitor that recognized trypsin, chymatrypsiri artd trypsiri-like proteinases, but it also recognized aspartic acid proteiriases fiom diflerent irisects. ft contairted no carbohydmte residues and showed a ltiglt stability at 96C at low pH.

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Research paper thumbnail of Purification and characterization of a glycoprotein α-amylase inhibitor from tepary bean seeds (Phaseolus acutifolius A. Gray)

Journal of Plant Physiology, 1996

ABSTRACT An α-amylase inhibitor was extracted and purified from tepary bean seeds. It is a 37,700... more ABSTRACT An α-amylase inhibitor was extracted and purified from tepary bean seeds. It is a 37,700 u (Da) glycoprotein determined by electrophoresis. The carbohydrate moiety (17%), determined by gas chromatography-mass spectrometry, consisted mainly of glucose and mannose, with small quantities of 2-acetamide-2-deoxy-glucose, acetyl-xylose, and xylose. This inhibitor is similar in several characteristics to previous a-amylase inhibitors studied in other bean seeds, including its molecular mass and its isoelectric point (4.7). The specificity toward different insect amylases was particulary high for the amylases from two bruchids, which are important pests to common beans (Z. subfasciatus and C. maculatus). This provides an interesting possibility for increasing resistance in common beans to attack by these pests. Its thermal stability was low even at temperatures as low as 60 °C.

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Research paper thumbnail of EGFR and p38MAPK Contribute to the Apoptotic Effect of the Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) in Colon Cancer Cells

Pharmaceuticals, Feb 14, 2023

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Research paper thumbnail of Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins

Glycoconjugate Journal, Nov 17, 2022

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Research paper thumbnail of Potencial y Algunos de los Mecanismos de Acción de los Hongos Entomopatógenos para el Control de Insectos Plaga Potencial y Algunos de los Mecanismos de Acción de los Hongos Entomopatógenos para el Control de Insectos Plaga

DOAJ (DOAJ: Directory of Open Access Journals), Feb 1, 2012

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Research paper thumbnail of Proteínas Involucradas en los Mecanismos de Defensa de Plantas Proteínas Involucradas en los Mecanismos de Defensa de Plantas

DOAJ (DOAJ: Directory of Open Access Journals), Feb 1, 2012

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Research paper thumbnail of Purification, Characterization, and Complete Amino Acid Sequence of a Trypsin Inhibitor from Amaranth (Amaranthus hypochondriacus) Seeds

Plant Physiology, Dec 1, 1993

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Research paper thumbnail of Crystal structure of Grenache (Vitis vinifera) Polyphenol Oxidase

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Research paper thumbnail of Acute toxicity evaluation of a tepary bean (Phaseolus acutifolius) lectin

The FASEB Journal, Mar 1, 2008

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Research paper thumbnail of Tepary bean lectins induces apoptosis on human colon cancer cells and inhibits early tumorogenesis in rats

The FASEB Journal

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Research paper thumbnail of A novel alpha-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds

Journal of Biological Chemistry, 1994

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Research paper thumbnail of Protease Inhibitors Present in Opuntia SPP

Acta Horticulturae, 2009

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Research paper thumbnail of Effect of protein fractions isolated from cereal grains on the development and survival of the cowpea weevil, Callosobruchus maculatus

International Journal of Tropical Insect Science, 1995

ABSTRACT The effect of protein fractions isolated from cereal grains on the development and survi... more ABSTRACT The effect of protein fractions isolated from cereal grains on the development and survival of the bean weevil, Callosobruchus maculatus, was investigated using wheat, rice and maize. These cereals were fractionated into albumins, globulins, prolamins, glutelins and a residue. The fractions were incorporated in artificial seeds which were infested by C. maculatus. Survival (as indicated by LD50) and development (as indicated by WD50) of 20-day-larvae of the bruchid were negatively affected by most of the fractions. Rice globulins were the most detrimental and affected both the survival and development of the larvae, while wheat albumins and globulins had a stronger effect on larval development than on survival. A previously isolated maize α-amylase/trypsin inhibitor showed an LD50 of 1.5% to Callosobruchus maculatus.RésuméL&#39;effet des fractions protéiniques des semences de blé de riz et de maïs a été étudié sur le développement et la survie du bruchide du niébé, Callosobruchus maculatus. Les céréales ont été fractionnées en albumines, globulines, prolamines, glutélines et en un résidu, avec les fractions incorporées dans des semences artificielles infestées du bruchide, Callosobruchus maculatus. La survie (telle qu&#39;indiquée par la CL50) et le développement (tel qu&#39;indiqué par WD50) des larves a âgées de 20 jours ont été négativement affectés par la plupart des fractions. Les globulines du riz ont été les plus nocives, affectant à la fois la survie et le développement des larves, tandis que l&#39;effet des albumines et de globulines du blé a été plus marqué sur le développement larvaire que sur la survie. Un inhibiteur de la α-amylase/trypsine auparavant isolé du maïs, a révélé une CL50 de 1,5% sur Callosobruchus maculatus.

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Research paper thumbnail of Generation means analysis of phenolic compounds in maize grain and susceptibility to maize weevil Sitophilus zeamais infestation

Canadian Journal of Botany, 1993

Generations derived from crosses between inbred lines were used to analyze the genetics of hydrox... more Generations derived from crosses between inbred lines were used to analyze the genetics of hydroxycinnamic acid accumulation in maize grain and susceptibility to infestation by the maize weevil Sitophilus zeamais. There was a significant negative correlation between phenolic content and susceptibility of the grain to weevil infestation, and the estimates of genetic parameters indicate that a close relationship between those two traits exists. Key words: insect resistance, maize, maize weevil, genetics, phenolics.

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Research paper thumbnail of In vitro effects of semipure protease inhibitor fractions from edible seeds on malignant cell survival

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Research paper thumbnail of Genetics of maize grain resistance to maize weevil

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Research paper thumbnail of Exclusive rewards in mutualisms: ant proteases and plant protease inhibitors create a lock-key system to protect<i>Acacia</i>food bodies from exploitation

Molecular Ecology, May 17, 2013

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Research paper thumbnail of Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins

Glycoconjugate Journal, Nov 17, 2022

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Research paper thumbnail of Intestinal permeability and bioaccessibility of a recombinant lectin from Tepary bean ( Phaseolus acutifolius ) using the everted intestine assay

The FASEB Journal, 2021

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Research paper thumbnail of Vicilin Storage Proteins from <i>Vigna unguiculata </i>(Legume) Seeds Inhibit Fungal Growth

Journal of Agricultural and Food Chemistry, Oct 1, 1997

ABSTRACT Vicilin storage proteins (7S globulins) isolated from Vigna unguiculata (cowpea) seeds w... more ABSTRACT Vicilin storage proteins (7S globulins) isolated from Vigna unguiculata (cowpea) seeds were shown to interfere with the germination of spores or conidia of the fungi Fusarium solani, Fusarium axysporum, Colletotrichum musae, Phytophtora capsici, Neurospora crassa, and Ustilago maydis sporidia. Cowpea vicilins have been shown to bind to fungal structures, possibly chitin-containing structures of the cell wall, and can be desorbed by strong acid. The results presented in this paper are in agreement with data previously obtained on the chitin-binding properties of cowpea vicilins and the effect they exert on the development and survival of the storage pest insect Callosobruchus maculatus (Coleoptera: Bruchidae).

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Research paper thumbnail of Purification and Partial Characterization of a Proteinase Inhibitor from Tepary Bean (Phaseolus Acutifolius) Seeds

Journal of Food Biochemistry, Oct 1, 1997

hi a qitnlitntive screeriing of 36 arcessions of tepary beam seeds, al1 tlie accessions inliibit ... more hi a qitnlitntive screeriing of 36 arcessions of tepary beam seeds, al1 tlie accessions inliibit tlte activity of bovirie trypsin and trypsin-like proteinases from the insect P. truncatus, arid tlie rnajorip of tliem inhibir a-amylase activity of severa1 importarit hect pests. A protein proteirrci-. inhibitor was purified from accessiori L-242-45, usirtg fracriorial precipitation, gelfiltration, ion encltange cfrromatography artd reverse-pltase HPLC. ilre protein showed un apparent molecular weigltt of 7,lW by PACE. However, corrtrary to other inhibitors previously reported, tlte inliibitory activity was only presenr iri tlre trimeric form. 7he protein was clraracterized as a serine-proteinase inliibitor that recognized trypsin, chymatrypsiri artd trypsiri-like proteinases, but it also recognized aspartic acid proteiriases fiom diflerent irisects. ft contairted no carbohydmte residues and showed a ltiglt stability at 96C at low pH.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Purification and characterization of a glycoprotein α-amylase inhibitor from tepary bean seeds (Phaseolus acutifolius A. Gray)

Journal of Plant Physiology, 1996

ABSTRACT An α-amylase inhibitor was extracted and purified from tepary bean seeds. It is a 37,700... more ABSTRACT An α-amylase inhibitor was extracted and purified from tepary bean seeds. It is a 37,700 u (Da) glycoprotein determined by electrophoresis. The carbohydrate moiety (17%), determined by gas chromatography-mass spectrometry, consisted mainly of glucose and mannose, with small quantities of 2-acetamide-2-deoxy-glucose, acetyl-xylose, and xylose. This inhibitor is similar in several characteristics to previous a-amylase inhibitors studied in other bean seeds, including its molecular mass and its isoelectric point (4.7). The specificity toward different insect amylases was particulary high for the amylases from two bruchids, which are important pests to common beans (Z. subfasciatus and C. maculatus). This provides an interesting possibility for increasing resistance in common beans to attack by these pests. Its thermal stability was low even at temperatures as low as 60 °C.

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Research paper thumbnail of EGFR and p38MAPK Contribute to the Apoptotic Effect of the Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) in Colon Cancer Cells

Pharmaceuticals, Feb 14, 2023

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Research paper thumbnail of Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins

Glycoconjugate Journal, Nov 17, 2022

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Research paper thumbnail of Potencial y Algunos de los Mecanismos de Acción de los Hongos Entomopatógenos para el Control de Insectos Plaga Potencial y Algunos de los Mecanismos de Acción de los Hongos Entomopatógenos para el Control de Insectos Plaga

DOAJ (DOAJ: Directory of Open Access Journals), Feb 1, 2012

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Research paper thumbnail of Proteínas Involucradas en los Mecanismos de Defensa de Plantas Proteínas Involucradas en los Mecanismos de Defensa de Plantas

DOAJ (DOAJ: Directory of Open Access Journals), Feb 1, 2012

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Research paper thumbnail of Purification, Characterization, and Complete Amino Acid Sequence of a Trypsin Inhibitor from Amaranth (Amaranthus hypochondriacus) Seeds

Plant Physiology, Dec 1, 1993

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Research paper thumbnail of Crystal structure of Grenache (Vitis vinifera) Polyphenol Oxidase

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Acute toxicity evaluation of a tepary bean (Phaseolus acutifolius) lectin

The FASEB Journal, Mar 1, 2008

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Research paper thumbnail of Tepary bean lectins induces apoptosis on human colon cancer cells and inhibits early tumorogenesis in rats

The FASEB Journal

Bookmarks Related papers MentionsView impact

Research paper thumbnail of A novel alpha-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds

Journal of Biological Chemistry, 1994

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Research paper thumbnail of Protease Inhibitors Present in Opuntia SPP

Acta Horticulturae, 2009

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effect of protein fractions isolated from cereal grains on the development and survival of the cowpea weevil, Callosobruchus maculatus

International Journal of Tropical Insect Science, 1995

ABSTRACT The effect of protein fractions isolated from cereal grains on the development and survi... more ABSTRACT The effect of protein fractions isolated from cereal grains on the development and survival of the bean weevil, Callosobruchus maculatus, was investigated using wheat, rice and maize. These cereals were fractionated into albumins, globulins, prolamins, glutelins and a residue. The fractions were incorporated in artificial seeds which were infested by C. maculatus. Survival (as indicated by LD50) and development (as indicated by WD50) of 20-day-larvae of the bruchid were negatively affected by most of the fractions. Rice globulins were the most detrimental and affected both the survival and development of the larvae, while wheat albumins and globulins had a stronger effect on larval development than on survival. A previously isolated maize α-amylase/trypsin inhibitor showed an LD50 of 1.5% to Callosobruchus maculatus.RésuméL&#39;effet des fractions protéiniques des semences de blé de riz et de maïs a été étudié sur le développement et la survie du bruchide du niébé, Callosobruchus maculatus. Les céréales ont été fractionnées en albumines, globulines, prolamines, glutélines et en un résidu, avec les fractions incorporées dans des semences artificielles infestées du bruchide, Callosobruchus maculatus. La survie (telle qu&#39;indiquée par la CL50) et le développement (tel qu&#39;indiqué par WD50) des larves a âgées de 20 jours ont été négativement affectés par la plupart des fractions. Les globulines du riz ont été les plus nocives, affectant à la fois la survie et le développement des larves, tandis que l&#39;effet des albumines et de globulines du blé a été plus marqué sur le développement larvaire que sur la survie. Un inhibiteur de la α-amylase/trypsine auparavant isolé du maïs, a révélé une CL50 de 1,5% sur Callosobruchus maculatus.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Generation means analysis of phenolic compounds in maize grain and susceptibility to maize weevil Sitophilus zeamais infestation

Canadian Journal of Botany, 1993

Generations derived from crosses between inbred lines were used to analyze the genetics of hydrox... more Generations derived from crosses between inbred lines were used to analyze the genetics of hydroxycinnamic acid accumulation in maize grain and susceptibility to infestation by the maize weevil Sitophilus zeamais. There was a significant negative correlation between phenolic content and susceptibility of the grain to weevil infestation, and the estimates of genetic parameters indicate that a close relationship between those two traits exists. Key words: insect resistance, maize, maize weevil, genetics, phenolics.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of In vitro effects of semipure protease inhibitor fractions from edible seeds on malignant cell survival

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Genetics of maize grain resistance to maize weevil

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Exclusive rewards in mutualisms: ant proteases and plant protease inhibitors create a lock-key system to protect<i>Acacia</i>food bodies from exploitation

Molecular Ecology, May 17, 2013

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins

Glycoconjugate Journal, Nov 17, 2022

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Intestinal permeability and bioaccessibility of a recombinant lectin from Tepary bean ( Phaseolus acutifolius ) using the everted intestine assay

The FASEB Journal, 2021

Bookmarks Related papers MentionsView impact