Alexander Bershadsky - Academia.edu (original) (raw)
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Papers by Alexander Bershadsky
arXiv (Cornell University), Aug 5, 2010
Molecular Biology of the Cell, Aug 15, 2011
Journal of Cell Science, Mar 1, 1994
Cells Tissues Organs, 1995
Adhesion of cells to their neighbors or to the extracellular matrix has multiple effects on cell ... more Adhesion of cells to their neighbors or to the extracellular matrix has multiple effects on cell shape, dynamics and fate. The most obvious and direct one is the assembly of single cells into ordered multicellular tissues and organs. This process requires specific transmembrane adhesion molecules which mediate the binding to the external surface, cytoskeletal filaments which attach to the cytoplasmic faces of the adhesion site, and a submembrane plaque which interconnects the two. The co-assembly of these junctional domains is essential for the formation of stable cell adhesions with the proper mechanical properties. In addition, adhesive interactions have prominent, global consequences on cell behavior and fate, affecting such processes as differentiation, growth and survival. To gain insight into the molecular basis for both the local and global effects of adhesive interactions, we have chosen to focus on one specific junctional domain, the submembrane plaque of microfilament-bound adhesions, namely cell-cell and cell-matrix adherens junctions. Based on both biochemical and morphological evidence we would like to propose that the junctional plaque plays a key role in mediating and regulating transmembrane junctional interactions and adhesion-dependent signaling. It offers multiple modes of linkage between the cytoskeleton and the membrane, and its assembly can be controlled at either the biosynthetic or posttranslational levels. Furthermore, recent data demonstrate that the submembrane plaque is involved in the transduction of transmembrane signals. We will show that this structure is the residence of an array of signaling enzymes (mostly kinases), that its structure and composition may be affected by activation of various signaling systems, and that adhesion itself may activate specific signal transduction pathways.
Annual Review of Cell Biology, Nov 1, 1991
Current Biology, Oct 1, 1996
Nature Reviews Molecular Cell Biology, 2009
Nature Cell Biology, Mar 1, 2017
Cell Reports, Mar 1, 2017
Biophysical Journal, 2012
Nature Materials, Oct 18, 2021
Investigative Ophthalmology & Visual Science, May 1, 2004
European Journal of Cell Biology, Mar 1, 2005
bioRxiv (Cold Spring Harbor Laboratory), Jun 15, 2020
Adherent filopodia are elongated finger-like membrane protrusions, extending from the edges of di... more Adherent filopodia are elongated finger-like membrane protrusions, extending from the edges of diverse cell types and participating in cell adhesion, spreading, migration and environmental sensing. The formation and elongation of filopodia are driven by the polymerization of parallel actin filaments, comprising the filopodia cytoskeletal core. Here, we report that adherent filopodia, formed during the spreading of cultured cells on galectin-8-coated substrates, tend to change the direction of their extension in a chiral fashion, acquiring a left-bent shape. Cryo-electron tomography examination indicated that turning of the filopodia tip to the left is accompanied by the displacement of the actin core bundle to the right of the filopodia midline. Reduction of the adhesion to galectin-8 by treatment with thiodigalactoside abolished the filopodia chirality. By modulating the expression of a variety of actin-associated filopodia proteins, we identified myosin-X and formin DAAM1 as major...
Molecular Biology of the Cell, Oct 1, 1999
arXiv (Cornell University), Aug 5, 2010
Molecular Biology of the Cell, Aug 15, 2011
Journal of Cell Science, Mar 1, 1994
Cells Tissues Organs, 1995
Adhesion of cells to their neighbors or to the extracellular matrix has multiple effects on cell ... more Adhesion of cells to their neighbors or to the extracellular matrix has multiple effects on cell shape, dynamics and fate. The most obvious and direct one is the assembly of single cells into ordered multicellular tissues and organs. This process requires specific transmembrane adhesion molecules which mediate the binding to the external surface, cytoskeletal filaments which attach to the cytoplasmic faces of the adhesion site, and a submembrane plaque which interconnects the two. The co-assembly of these junctional domains is essential for the formation of stable cell adhesions with the proper mechanical properties. In addition, adhesive interactions have prominent, global consequences on cell behavior and fate, affecting such processes as differentiation, growth and survival. To gain insight into the molecular basis for both the local and global effects of adhesive interactions, we have chosen to focus on one specific junctional domain, the submembrane plaque of microfilament-bound adhesions, namely cell-cell and cell-matrix adherens junctions. Based on both biochemical and morphological evidence we would like to propose that the junctional plaque plays a key role in mediating and regulating transmembrane junctional interactions and adhesion-dependent signaling. It offers multiple modes of linkage between the cytoskeleton and the membrane, and its assembly can be controlled at either the biosynthetic or posttranslational levels. Furthermore, recent data demonstrate that the submembrane plaque is involved in the transduction of transmembrane signals. We will show that this structure is the residence of an array of signaling enzymes (mostly kinases), that its structure and composition may be affected by activation of various signaling systems, and that adhesion itself may activate specific signal transduction pathways.
Annual Review of Cell Biology, Nov 1, 1991
Current Biology, Oct 1, 1996
Nature Reviews Molecular Cell Biology, 2009
Nature Cell Biology, Mar 1, 2017
Cell Reports, Mar 1, 2017
Biophysical Journal, 2012
Nature Materials, Oct 18, 2021
Investigative Ophthalmology & Visual Science, May 1, 2004
European Journal of Cell Biology, Mar 1, 2005
bioRxiv (Cold Spring Harbor Laboratory), Jun 15, 2020
Adherent filopodia are elongated finger-like membrane protrusions, extending from the edges of di... more Adherent filopodia are elongated finger-like membrane protrusions, extending from the edges of diverse cell types and participating in cell adhesion, spreading, migration and environmental sensing. The formation and elongation of filopodia are driven by the polymerization of parallel actin filaments, comprising the filopodia cytoskeletal core. Here, we report that adherent filopodia, formed during the spreading of cultured cells on galectin-8-coated substrates, tend to change the direction of their extension in a chiral fashion, acquiring a left-bent shape. Cryo-electron tomography examination indicated that turning of the filopodia tip to the left is accompanied by the displacement of the actin core bundle to the right of the filopodia midline. Reduction of the adhesion to galectin-8 by treatment with thiodigalactoside abolished the filopodia chirality. By modulating the expression of a variety of actin-associated filopodia proteins, we identified myosin-X and formin DAAM1 as major...
Molecular Biology of the Cell, Oct 1, 1999