Alexander Lukatkin - Academia.edu (original) (raw)

Papers by Alexander Lukatkin

Chilling temperatures (1-10ºC) lead to numerous physiological disturbances in the cells of chilli... more Chilling temperatures (1-10ºC) lead to numerous physiological disturbances in the cells of chilling-sensitive plants and result in chilling injury and death of tropical and subtropical plants, e.g., many vegetable species. The literature review shows that the exposure of chilling-sensitive plants to low temperatures causes disturbances in all physiological processes -water regime, mineral nutrition, photosynthesis, respiration and metabolism. Inactivation of metabolism, observed at chilling of chilling-sensitive plants is a complex function of both temperature and duration of exposure. Response of plants to low temperature exposure is associated with a change in the rate of gene transcription of a number of low molecular weight proteins. The review analyzes historical aspects in the development of ideas about the nature of chilling damage of chillingsensitive plants and direction of modern research. Based on the authors' own research and the literature data, the concept of cold damage was proposed, which highlighted the leading role of oxidative stress in the induction of stress response. According to this concept there were distinguished possible ways how to improve cold tolerance. They were divided into several groups: the thermal effect (low-temperature hardening, thermal conditioning, intermediate warming and the effect of heat stress), chemical treatment (trace elements, synthetic growth regulators, antioxidants) and the use of gene and cell engineering.

Environmental science and pollution research international, Jan 20, 2015

This work investigated the accumulation, allocation, and impact of zinc (Zn; 1.0 μM-10 mM) in mai... more This work investigated the accumulation, allocation, and impact of zinc (Zn; 1.0 μM-10 mM) in maize (Zea mays L.) seedlings under simulated laboratory conditions. Z. mays exhibited no significant change in its habitus (the physical characteristics of plants) up to 10-1000 μM of Zn (vs 5-10 mM Zn). Zn tolerance evaluation, based on the root test, indicated a high tolerance of Z. mays to both low and intermediate (or relatively high) concentrations of Zn, whereas this plant failed to tolerate 10 mM Zn and exhibited a 5-fold decrease in its Zn tolerance. Contingent to Zn treatment levels, Zn hampered the growth of axial organs and brought decreases in the leaf area, water regime, and biomass accumulation. Nevertheless, at elevated levels of Zn (10 mM), Zn(2+) was stored in the root cytoplasm and inhibited both axial organ growth and water regime. However, accumulation and allocation of Zn in Z. mays roots, studied herein employing X-ray fluorimeter and histochemical methods, were close...

Russian Agricultural Sciences, 2011

ABSTRACT The effect of low (3°C) and high (44°C) temperatures on stress indices in young maize pl... more ABSTRACT The effect of low (3°C) and high (44°C) temperatures on stress indices in young maize plants is examined. Unfavorable temperatures cause inhibition of the growth of axial organs, intensification of lipid peroxidation, and reduction of antioxidant activity, from the degree of suppression of which the intensity of the stress effect of abiotic factors on plants can be judged.

Russian Agricultural Sciences, 2009

ABSTRACT Data are given on the effect of various exogenous growth regulators on the degree of chi... more ABSTRACT Data are given on the effect of various exogenous growth regulators on the degree of chilling injury of chilling-sensitive plants. A decrease of damage in cucumber and maize leaves in the case of treating seeds or seedlings with cytokinin-like preparations and chlorocholine chloride is shown. A conclusion is made about the possibility of the practical use of synthetic growth regulators for increasing chilling resistance of plants.

Russian Agricultural Sciences, 2010

ABSTRACT The production of maize callus lines and their use for assessing chilling injury are des... more ABSTRACT The production of maize callus lines and their use for assessing chilling injury are described. The substantial influence of physiological factors (type of explant, age of plant and culture) on the stress resistance of cells is shown. It is concluded that maize callus cultures can be used as a model object when studying stress resistance at the cellular level. Key wordsmaize-callus cultures-resistance-chilling stress

The impact of heavy metals (HM) on the growth pattern of maize (Zea mais L. cv. 'Krasnodarskij 16... more The impact of heavy metals (HM) on the growth pattern of maize (Zea mais L. cv. 'Krasnodarskij 167') seedlings was examined. Seedling growth was tested under physiological and sublethal doses of HM, including Pb, Cu, Ni and Zn. Physiological 10-100 mM doses stimulated the growth of young plants, while 1-5 µM induced suppression of growth processes and biomass accumulation (especially for roots) as well as reduction of leaf area and water disbalance. The extent of such effect depended on the chemical nature and concentration of a heavy metal.

Environmental research, Jan 4, 2015

Nanoscale copper particles (nano-Cu) are used in many antimicrobial formulations and products for... more Nanoscale copper particles (nano-Cu) are used in many antimicrobial formulations and products for their antimicrobial activity. They may enter deliberately and/or accidentally into terrestrial environments including soils. Being the major 'eco-receptors' of nanoscale particles in the terrestrial ecosystem, soil-microbiota and plants (the soil-plant system) have been used as a model to dissect the potential impact of these particles on the environmental and human health. In the soil-plant system, the plant can be an indirect non-target organism of the soil-associated nano-Cu that may in turn affect plant-based products and their consumers. By all accounts, information pertaining to nano-Cu toxicity and the underlying potential mechanisms in the soil-plant system remains scanty, deficient and little discussed. Therefore, based on some recent reports from (bio)chemical, molecular and genetic studies of nano-Cu versus soil-plant system, this article: (i) overviews the status, ch...

Frontiers in Environmental Science, 2014

Chilling temperatures (1-10 • C) are known to disturb cellular physiology, cause oxidative stress... more Chilling temperatures (1-10 • C) are known to disturb cellular physiology, cause oxidative stress via creating imbalance between generation and metabolism of reactive oxygen species (ROS) leading finally to cell and/or plant death. Owing to known significance of low molecular antioxidants-ascorbic acid (AsA) and glutathione (GSH) in plant stress-tolerance, this work analyzes the role of exogenously applied AsA and GSH in the alleviation of chilling stress (3 • C)-impact in cucumber (Cucumis sativus L. cv. Vjaznikowskij 37) plants. Results revealed AsA and GSH concentration dependent metabolism of ROS such as superoxide (O 2 • − ) and the mitigation of ROS-effects such as lipid peroxidation (LPO) as well as membrane permeability (measured as electrolyte leakage) in C. sativus leaf discs. AsA concentration (750 µM) and GSH (100 µM) exhibited maximum reduction in O 2 • − generation, LPO intensity as well as electrolyte leakage, all of these were increased in cold water (3 and 25 • C)-treated leaf discs. However, AsA, in particular, had a pronounced antioxidative effect, more expressed in case of leaf discs during chilling (3 • C); whereas, at temperature 25 • C, some AsA concentrations (such as 50 and 100 mM AsA) exhibited a prooxidative effect that requires molecular-genetic studies. Overall, it is inferred that AsA and GSH have high potential for sustainably increasing chilling-resistance in plants.

Environmental science and pollution research international, Jan 5, 2014

Stress factors provoke enhanced production of reactive oxygen species (ROS) in plants. ROS that e... more Stress factors provoke enhanced production of reactive oxygen species (ROS) in plants. ROS that escape antioxidant-mediated scavenging/detoxification react with biomolecules such as cellular lipids and proteins and cause irreversible damage to the structure of these molecules, initiate their oxidation, and subsequently inactivate key cellular functions. The lipid- and protein-oxidation products are considered as the significant oxidative stress biomarkers in stressed plants. Also, there exists an abundance of information on the abiotic stress-mediated elevations in the generation of ROS, and the modulation of lipid and protein oxidation in abiotic stressed plants. However, the available literature reflects a wide information gap on the mechanisms underlying lipid- and protein-oxidation processes, major techniques for the determination of lipid- and protein-oxidation products, and on critical cross-talks among these aspects. Based on recent reports, this article (a) introduces ROS an...

Pesticide Biochemistry and Physiology, 2013

Leaf disks as well as intact 7-day-old plants of winter wheat (Triticum aestivum L., cv. Mironovs... more Leaf disks as well as intact 7-day-old plants of winter wheat (Triticum aestivum L., cv. Mironovskaya 808), winter rye (Secale cereale L., cv. Estafeta Tatarstana), and maize (Zea mays L., cv. Kollektivnyi 172MV), were treated with the aryloxyphenoxypropionate class herbicide TOPIK, concentrate-emulsion (active ingredient is clodinafop-propargyl (CP), 8-800μg/L), and the effects of short-term action (up to 3h) and long-term aftereffect (up to 3days) on physiological and biochemical indices related to oxidative stress development were studied. The herbicide induced changes, predominantly increases in lipid peroxidation (LPO) intensity, superoxide anion O2(-) generation, total antioxidant activity (AOA), and catalase (CAT) and ascorbate peroxidase (APOX) activity, although the response by plants was nonlinear and depended on the herbicide concentration and duration of treatment. The highest level of generation of O2(-) was observed in the leaves of maize and winter wheat treated by 800μg/L CP, both in the short- and long-term. As TOPIK concentration increased, so too did LPO and AOA in leaves, confirming the presence of oxidative stress in the cells of all three cereals. Antioxidant enzymes were most active in winter rye and wheat, and least active in maize indicating a protective antioxidant mechanism in the first two cereals.

Folia Horticulturae, 2000

The effects of different concentrations of copper ions (Cu 2+ in the form of CuSO4 × 5H2O) on in ... more The effects of different concentrations of copper ions (Cu 2+ in the form of CuSO4 × 5H2O) on in vivo cucumber (Cucumis sativus L. 'Edinstvo') seedlings as well as on in vitro hypocotyl-derived callus were considered. Callus induction from hypocotyls was more prolific than from roots or cotyledons. Thus, callus obtained from hypocotyls of 7-day-old cucumber plants was cultured for 5 weeks on Murashige and Skoog medium containing 4 mg dm -3 2,4-D + 1 mg dm -3 BA supplemented with 0.01 mM, 0.1 mM or 1.0 mM of Cu 2+ . Biochemical indices related to oxidative stress were assessed. Cu 2+ at 0.01 mM stimulated callus induction but 1.0 mM Cu 2+ negatively affected callus formation and growth. LPO intensity was significantly lower than the control at all concentrations of Cu 2+ but significantly higher than the control in plants exposed to 0.01 or 0.1 mM Cu 2+ . A similar trend was observed for the generation of the superoxide radical in both callus and plantlets. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APOX) activity increased in both callus and plantlets, but the level of increase in these antioxidant enzyme systems depended on the Cu 2+ concentration. Cu 2+ ions had a stronger (i.e., more negative) influence on oxidative stress in cucumber seedlings than on cucumber callus.

Journal of Trace Elements in Medicine and Biology, 2014

The generation of superoxide radicals, lipid peroxidation (as measured by malone dialdehyde forma... more The generation of superoxide radicals, lipid peroxidation (as measured by malone dialdehyde formation) and the activity of selected antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase) were assessed in radish (Raphanus sativus L.), in response to elevated concentrations of copper ions in the culture medium in vitro and in vivo. Experiments were performed on 7-day-old seedlings and 5-week-old calluses grown on media supplemented with CuSO4 in concentrations of 10, 100 and 1000μМ. The exposure to elevated Cu concentrations in the medium significantly reduced both callogenesis and the proliferation of radish calluses in vitro. Cu treatment resulted in the increased generation of the superoxide radical (O2(-)) in radish seedlings and calluses indicating the occurrence of oxidative stress in radish cells, whereas the level of lipid peroxidation (LPO) remained unchanged. Both in calluses and in radish seedlings in vivo, the relative level of oxidative stress was maximal at micromolar Cu concentrations and became attenuated with increasing Cu concentrations. Stronger oxidative stress occurred in the radish seedlings in vivo, compared with radish calluses in vitro. The observed lower sensitivity of calluses to Cu-induced oxidative stress and their ability to proliferate upon exposure to Cu concentrations of up to 1000μМ demonstrate the potential of in vitro cell-selection to obtain metal-tolerant radish plant lines.