Alexander Uskov - Academia.edu (original) (raw)
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Papers by Alexander Uskov
Arxiv preprint arXiv:1109.1869, 2011
Abstract: A. Brodsky and Yu. Gurevitch approach is discussed and generalized for photoemission fr... more Abstract: A. Brodsky and Yu. Gurevitch approach is discussed and generalized for photoemission from metal nano-particles taking into account the excitation of localized plasmon resonance (LPR) and changes of electromagnetic field (EMF) and conduction ...
Applied Microbiology and Biotechnology, 2012
A test system is described and expanded upon for mass field immunochromatography assay on porous ... more A test system is described and expanded upon for mass field immunochromatography assay on porous membrane carriers for rapid diagnostics of potato virus X (PVX) in potato leaf tissue and sprout extracts using colloidal gold nanoparticles as a marker. Sensitivity of the assay developed for PVX identification is found to be comparable to the sensitivity of solid-phase sandwich-ELISA. Complete assay time does not exceed 15 min, and the lower limit of the PVX detection in non-clarified leaf extract is 2 ng/ml. A single measurement requires 0.1-0.2 ml (3-5 drops) of tested solution only (extracted from 10-20 mg of potato leaf tissue or sprouts). The simplicity and reliability of the method makes it especially efficient in direct rapid monitoring of many infected potato specimens in the field, as verified by field trials of 360 clones of 28 domestic and foreign cultivars of potato. A diagnostic kit for routine analyses of potato viral infections both in the laboratory and in the field is described and expanded upon.
Arxiv preprint arXiv:1109.1869, 2011
Abstract: A. Brodsky and Yu. Gurevitch approach is discussed and generalized for photoemission fr... more Abstract: A. Brodsky and Yu. Gurevitch approach is discussed and generalized for photoemission from metal nano-particles taking into account the excitation of localized plasmon resonance (LPR) and changes of electromagnetic field (EMF) and conduction ...
Applied Microbiology and Biotechnology, 2012
A test system is described and expanded upon for mass field immunochromatography assay on porous ... more A test system is described and expanded upon for mass field immunochromatography assay on porous membrane carriers for rapid diagnostics of potato virus X (PVX) in potato leaf tissue and sprout extracts using colloidal gold nanoparticles as a marker. Sensitivity of the assay developed for PVX identification is found to be comparable to the sensitivity of solid-phase sandwich-ELISA. Complete assay time does not exceed 15 min, and the lower limit of the PVX detection in non-clarified leaf extract is 2 ng/ml. A single measurement requires 0.1-0.2 ml (3-5 drops) of tested solution only (extracted from 10-20 mg of potato leaf tissue or sprouts). The simplicity and reliability of the method makes it especially efficient in direct rapid monitoring of many infected potato specimens in the field, as verified by field trials of 360 clones of 28 domestic and foreign cultivars of potato. A diagnostic kit for routine analyses of potato viral infections both in the laboratory and in the field is described and expanded upon.