Alexia Mello - Academia.edu (original) (raw)

Papers by Alexia Mello

Experimental Parasitology

Experimental Parasitology

Disciplinarum Scientia - Ciências Naturais e Tecnológicas, 2020

Trichomoniasis, an infection caused by Trichomonas vaginalis, is the most commom non-viral sexual... more Trichomoniasis, an infection caused by Trichomonas vaginalis, is the most commom non-viral sexually transmitted infection worldwide. Due to Metronidazole's (MTZ) resistance to standard drugs, this study aimed to evaluate the efficacy of free and nanostructured essential oils (EO) as Cymbopogon flexuosus (lemongrass), Eucalyptus globulus (eucalyptus) and Ocimum basilicum (basil), besides antioxidant activities against T. vaginalis. The free and nanostructured EOs were tested for in vitro activity against Trichomonas vaginalis; trophozoites were treated with different concentrations (ranging from 0.25% to 1.5%) in order to establish the MIC and IC50 values. Oxidative stress was also evaluated by measuring lipid peroxidation by the detection of thiobarbituric acid reactive substances (TBARS). The MIC observed for lemongrass was 1.5% with free oil and 1.0% with nanoemulsion, while for basil it was 1.5% with free and nanostructured oils. Significant antiparasitic activities were observed in 12 and 24 hours exposure, respectively, inhibiting 100% of trophozoites. Antioxidant activity was also observed in these oils, which, either in free or nanostructured form, were able to reduce lipid peroxidation.

Revista Brasileira de Parasitologia Veterinária, 2019

Currently, albendazole is one of the most commonly used drugs because of its affordability. The o... more Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours ...

Semina: Ciências Agrárias

Trichomoniasis, caused by the protozoan Trichomonas gallinae, has as main hosts birds of the Colu... more Trichomoniasis, caused by the protozoan Trichomonas gallinae, has as main hosts birds of the Columbidae family, which have a high prevalence of the protozoan without manifesting the disease. The continuous growth of the pigeon population and its cosmopolitan nature mean that today there is a worldwide distribution of this species, being responsible for the distribution and maintenance of the prevalence of trichomoniasis in almost the entire world. The transmission of the disease may be by direct contact, or indirect, through food or water. This indirect route is the reason why such a wide range of bird orders can be infected, very different from columbids such as falconiformes, strigiformes, passerines, piciformes, psittaciformes, gruiformes, galliformes or anseriformes. Thus, the objective of this study was to evaluate the presence of T. gallinae in passerines received at a Wild Animal Screening Center. In order to carry out this study, 300 birds of the order Passerine correspondin...

International Journal of Medicinal Mushrooms, 2022

Revista Científica Eletrônica de Medicina Veterinária, 2020

Avicenna Journal of Phytomedicine, 2021

Objective: This study was done to evaluate the in vitro and in vivo effects of the essential oil ... more Objective: This study was done to evaluate the in vitro and in vivo effects of the essential oil (OE-CL) and nanoemulsion (N-CL) of Cymbopogon flexuosus against Trichomonas gallinae. Materials and Methods: In vitro assays were done with 106 parasites and OE-CL and N-CL in the concentrations: 110, 220, 330, 440, 550, 660, 770 and 880 µg/ml and four controls: CN (culture medium and trophozoites), MTZ (trophozoites plus 800 µg/ml of metronidazole), TW (trophozoites plus vehicles used for solubilization of derivatives (0.01% Tween) and NB (blank nanoemulsion 880 µg/ml). The in vivo assay was done in 35 quails (Coturnix coturnix) infected experimentally 4x104 mg/kg, were divided in seven groups (n=5): A (control–healthy), B (control infected), C (control TW 0.01%), D (NB 0.88 mg/kg), E (drug MTZ 25 mg/kg, F (OE-CL at 0.55 mg/kg) and G (N-CL at 0.44 mg/kg), during 7 consecutive days. Results: The in vitro test showed that the OE-CL (550 μg/ml) and N-CL (440 μg/ml) concentrations reduced t...

Experimental Parasitology

Experimental Parasitology

Disciplinarum Scientia - Ciências Naturais e Tecnológicas, 2020

Trichomoniasis, an infection caused by Trichomonas vaginalis, is the most commom non-viral sexual... more Trichomoniasis, an infection caused by Trichomonas vaginalis, is the most commom non-viral sexually transmitted infection worldwide. Due to Metronidazole's (MTZ) resistance to standard drugs, this study aimed to evaluate the efficacy of free and nanostructured essential oils (EO) as Cymbopogon flexuosus (lemongrass), Eucalyptus globulus (eucalyptus) and Ocimum basilicum (basil), besides antioxidant activities against T. vaginalis. The free and nanostructured EOs were tested for in vitro activity against Trichomonas vaginalis; trophozoites were treated with different concentrations (ranging from 0.25% to 1.5%) in order to establish the MIC and IC50 values. Oxidative stress was also evaluated by measuring lipid peroxidation by the detection of thiobarbituric acid reactive substances (TBARS). The MIC observed for lemongrass was 1.5% with free oil and 1.0% with nanoemulsion, while for basil it was 1.5% with free and nanostructured oils. Significant antiparasitic activities were observed in 12 and 24 hours exposure, respectively, inhibiting 100% of trophozoites. Antioxidant activity was also observed in these oils, which, either in free or nanostructured form, were able to reduce lipid peroxidation.

Revista Brasileira de Parasitologia Veterinária, 2019

Currently, albendazole is one of the most commonly used drugs because of its affordability. The o... more Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours ...

Semina: Ciências Agrárias

Trichomoniasis, caused by the protozoan Trichomonas gallinae, has as main hosts birds of the Colu... more Trichomoniasis, caused by the protozoan Trichomonas gallinae, has as main hosts birds of the Columbidae family, which have a high prevalence of the protozoan without manifesting the disease. The continuous growth of the pigeon population and its cosmopolitan nature mean that today there is a worldwide distribution of this species, being responsible for the distribution and maintenance of the prevalence of trichomoniasis in almost the entire world. The transmission of the disease may be by direct contact, or indirect, through food or water. This indirect route is the reason why such a wide range of bird orders can be infected, very different from columbids such as falconiformes, strigiformes, passerines, piciformes, psittaciformes, gruiformes, galliformes or anseriformes. Thus, the objective of this study was to evaluate the presence of T. gallinae in passerines received at a Wild Animal Screening Center. In order to carry out this study, 300 birds of the order Passerine correspondin...

International Journal of Medicinal Mushrooms, 2022

Revista Científica Eletrônica de Medicina Veterinária, 2020

Avicenna Journal of Phytomedicine, 2021

Objective: This study was done to evaluate the in vitro and in vivo effects of the essential oil ... more Objective: This study was done to evaluate the in vitro and in vivo effects of the essential oil (OE-CL) and nanoemulsion (N-CL) of Cymbopogon flexuosus against Trichomonas gallinae. Materials and Methods: In vitro assays were done with 106 parasites and OE-CL and N-CL in the concentrations: 110, 220, 330, 440, 550, 660, 770 and 880 µg/ml and four controls: CN (culture medium and trophozoites), MTZ (trophozoites plus 800 µg/ml of metronidazole), TW (trophozoites plus vehicles used for solubilization of derivatives (0.01% Tween) and NB (blank nanoemulsion 880 µg/ml). The in vivo assay was done in 35 quails (Coturnix coturnix) infected experimentally 4x104 mg/kg, were divided in seven groups (n=5): A (control–healthy), B (control infected), C (control TW 0.01%), D (NB 0.88 mg/kg), E (drug MTZ 25 mg/kg, F (OE-CL at 0.55 mg/kg) and G (N-CL at 0.44 mg/kg), during 7 consecutive days. Results: The in vitro test showed that the OE-CL (550 μg/ml) and N-CL (440 μg/ml) concentrations reduced t...