Ali Asadirad - Academia.edu (original) (raw)

Papers by Ali Asadirad

Iranian Journal of Allergy Asthma and Immunology, Jan 6, 2024

Inflammopharmacology, Mar 30, 2023

Chemokines belong to the family of cytokines with chemoattractant properties that regulate chemot... more Chemokines belong to the family of cytokines with chemoattractant properties that regulate chemotaxis and leukocyte migration, as well as the induction of angiogenesis and maintenance of hemostasis. Curcumin, the major component of the Curcuma longa rhizome, has various pharmacological actions, including anti-inflammatory, immune-regulatory, anti-oxidative, and lipid-modifying properties. Chemokines and chemokine receptors are influenced/modulated by curcumin. Thus, the current review focuses on the molecular mechanisms associated with curcumin's effects on chemoattractant cytokines, as well as putting into context the many studies that have reported curcumin-mediated regulatory effects on inflammatory conditions in the organs/systems of the body (e.g., the central nervous system, liver, and cardiovascular system). Curcumin's effects on viral and bacterial infections, cancer, and adverse pregnancy outcomes are also reviewed.

Phytotherapy Research, Jan 17, 2022

The present study conducted a placebo‐controlled clinical trial to evaluate the impact of nano‐cu... more The present study conducted a placebo‐controlled clinical trial to evaluate the impact of nano‐curcumin on the inflammatory cytokines in mild‐to‐moderate hospitalized COVID‐19 patients. A total of 60 COVID‐19 patients were randomly divided into nano‐curcumin and control groups, and then they received 240 mg/day nano‐curcumin for 7 days. The clinical manifestation and laboratory parameters in patients were recorded on days 0 and seven. Also, SYBR Green real‐time PCR and ELISA techniques were implicated in assessing the mRNA expression of IFN‐γ, IL‐1β, IL‐6, MCP‐1, and TNF‐α and the serum levels of IL‐1β, IL‐6, and TNF‐α inflammatory mediators, respectively. Although the clinical manifestations and laboratory parameters improved via the nano‐curcumin treatment, the mRNA expression of IFN‐γ (p = 0.006) and TNF‐α (p = 0.04) were significantly reduced. Besides, a considerable difference was observed between the nano‐curcumin and control groups in the expression of IFN‐γ (p = 0.001), IL‐1β (p = 0.0002), and IL‐6 (p = 0.008). In addition, there was a significant difference between the nano‐curcumin and control groups in the serum levels of IL‐1β (p = 0.042). The evidence demonstrated that nano‐curcumin could be implicated as a complementary medication to act as an antiinflammatory agent and inhibit inflammatory complications.

Immunobiology, May 1, 2023

International Immunopharmacology, Dec 1, 2022

International Immunopharmacology

Molecular and Cellular Probes, Aug 1, 2022

PubMed, Jun 1, 2019

Mesenchymal stem cells (MSCs) are well known due to their immunomodulatory effect, but the exact ... more Mesenchymal stem cells (MSCs) are well known due to their immunomodulatory effect, but the exact mechanisms have not been defined. Several studies demonstrated that the exerted immunoregulatory effect of these cells could be mediated by paracrine factors to illustrate, cytokines, chemokine, and among which, extracellular vesicles are one of them to play a crucial role. Moreover, it is assumed that extracellular vesicles are an essential player in intracellular communication by transferring their component. In this respect, the efficiency of conditioned media and exosomes was compared to illustrate a practical approach to cell-free based therapies. In the current study, we investigated the effect of both MSCs conditioned media (MSC-CM) and MSCs-derived exosomes on the expression of pro-inflammatory and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMCs). In this regard, isolated PBMCs were treated with MSC-CM and MSC-derived exosome as separated groups. Expression of inflammatory and anti-inflammatory markers was evaluated by Real-time PCR and ELISA. The immunoregulatory effect of MSC-CM on pro-inflammatory and anti-inflammatory genes, such as IL-12b, iNOS, EGR-2, IL-10 with an exception in case of IL-6 was more significant. Whereas in protein levels IL-10 showed the most substantial difference in exosome treated groups. It could be assumed that MSC-CM has more immunoregulatory impact on monocyte in contrast with exosomes.Taken together, by considering the recent approaches to cell-free therapy and the immunoregulatory impact of MSCs, yet relatively little is known about the efficacy of human-MSC-CM and secreted exosome compared with each other.

European Journal of Pharmacology, Sep 1, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Tissue & Cell, Aug 1, 2021

Tumor cells modulate immune responses by secreting exosomes. Tumor exosomes can affect the metabo... more Tumor cells modulate immune responses by secreting exosomes. Tumor exosomes can affect the metabolism of immune cells and increase immune inhibitory molecules such as programmed cell death protein 1 (PD-1). PD-1 inhibits the glycolysis pathway in immune cells. We investigated the role of tumor exosomes in how metabolic changes occur through the PD1-GLUT1-HK2 metabolic axisin peripheral blood mononuclear cells (PBMCs). The MDA-MB-231 cell line was cultured, serum samples from breast cancer patients were collected, and exosomes purified from serum samples and the MDA-MB-231 cell line. PBMCs were treated with purified exosomes for 72 h and, the expression of PD1-GLUT1-HK2 genes was measured by real-time PCR. Our study results showed relative expression of the HK2 gene in both groups treated with MDA-MB-231 cell line exosomes and serum exosomes of breast cancer patients was significantly increased compared to the control group (p < 0.0001). Also, the relative expression of the PD1 gene and GLUT1 gene showed a significant increase compared to the control group only in the group treated with MDA-MB-231 cell line exosomes (p < 0.0001). Therefore, Breast cancer exosomes increased the expression of key genes in the glycolysis pathway, increasing the glycolysis pathway in PBMCs. Increased expression of PD-1 could not prevent the expression of critical genes in the glycolysis pathway as in previous studies.

Inflammopharmacology

Chemokines belong to the family of cytokines with chemoattractant properties that regulate chemot... more Chemokines belong to the family of cytokines with chemoattractant properties that regulate chemotaxis and leukocyte migration, as well as the induction of angiogenesis and maintenance of hemostasis. Curcumin, the major component of the Curcuma longa rhizome, has various pharmacological actions, including anti-inflammatory, immune-regulatory, anti-oxidative, and lipid-modifying properties. Chemokines and chemokine receptors are influenced/modulated by curcumin. Thus, the current review focuses on the molecular mechanisms associated with curcumin's effects on chemoattractant cytokines, as well as putting into context the many studies that have reported curcumin-mediated regulatory effects on inflammatory conditions in the organs/systems of the body (e.g., the central nervous system, liver, and cardiovascular system). Curcumin's effects on viral and bacterial infections, cancer, and adverse pregnancy outcomes are also reviewed.

International Immunopharmacology

International Immunopharmacology, 2022

Nowadays, various strategies are considered to prime Dendritic cells (DCs) with tumor antigens. T... more Nowadays, various strategies are considered to prime Dendritic cells (DCs) with tumor antigens. The tumor cell-derived exosomes are recognized as one of the most efficient strategies for achieving this purpose. In this regard, MicroRNA 155 (miR-155) is employed as one of the most prominent miRNAs, which play substantial roles in DCs maturation and IL-12 production. This study investigates the tumor growth suppression and antitumor effects of DCs primed with miR-155-enriched exosome on the BALB/c murine model of colorectal cancer induced by CT-26 cell lines. Therefore, a holistic framework is proposed for the analysis procedure. In the first stage, miRNA-155 was electroporated into texosomes. In the second stage, bonemarrow-derived DCs were treated with miRNA-155 enriched texosomes. Then, antitumor properties of manipulated DC have been evaluated in the BALB/c mice model of colorectal cancer. After DC immunotherapy, several features have been assessed for each animal, including survival, body weight, tumor volume/size, histopathology, and serum cytokine levels. Also, flow cytometric evaluation has been performed for the spleen and the tumor tissue T-cell subsets. The findings demonstrated that the primed DCs could significantly increase IL-12p70 and IFN-γ in serum and accelerate the differentiation, proliferation, and cytotoxicity effects on the Th and CTL cells. Also, the treatment also increased the infiltration of Th and CTL cells into the tumor microenvironment while decreasing Tregs. This situation causes tumor growth control, and survival improvement. Therefore, DC immunotherapywith miR-155-enriched texosomes can be employed as a the desired approach for inducing antitumor immune responses, controlling tumor growth, and improving survival in mice with colorectal cancer. However, it is essential to perform more investigations to confirm the clinical application of this approach in humans and other types of tumors.

Molecular and Cellular Probes

Phytotherapy Research

The present study conducted a placebo‐controlled clinical trial to evaluate the impact of nano‐cu... more The present study conducted a placebo‐controlled clinical trial to evaluate the impact of nano‐curcumin on the inflammatory cytokines in mild‐to‐moderate hospitalized COVID‐19 patients. A total of 60 COVID‐19 patients were randomly divided into nano‐curcumin and control groups, and then they received 240 mg/day nano‐curcumin for 7 days. The clinical manifestation and laboratory parameters in patients were recorded on days 0 and seven. Also, SYBR Green real‐time PCR and ELISA techniques were implicated in assessing the mRNA expression of IFN‐γ, IL‐1β, IL‐6, MCP‐1, and TNF‐α and the serum levels of IL‐1β, IL‐6, and TNF‐α inflammatory mediators, respectively. Although the clinical manifestations and laboratory parameters improved via the nano‐curcumin treatment, the mRNA expression of IFN‐γ (p = 0.006) and TNF‐α (p = 0.04) were significantly reduced. Besides, a considerable difference was observed between the nano‐curcumin and control groups in the expression of IFN‐γ (p = 0.001), IL‐1β (p = 0.0002), and IL‐6 (p = 0.008). In addition, there was a significant difference between the nano‐curcumin and control groups in the serum levels of IL‐1β (p = 0.042). The evidence demonstrated that nano‐curcumin could be implicated as a complementary medication to act as an antiinflammatory agent and inhibit inflammatory complications.

Journal of Cellular Physiology, 2020

Extract of Echinops persicus is traditionally used for a long time in Iran for treatment of cough... more Extract of Echinops persicus is traditionally used for a long time in Iran for treatment of cough and constipation. This extract is produced by activity of bug (Situphilus spp.) on the plant. We documented its anti-tussive effect in rats in our previous study. The aim of this study was to assess the anti-ulcer effect of Echinops persicus in an animal model. In this study we evaluated anti-ulcer effect of Echinops persicus by Shay's method in rats. In 3 groups of rats, pylorus was ligatured under anesthesia. The rats were euthanized after 19 hours later and number and level of ulcer in stomach was measured. In group 2 the extract was orally administered 45 minutes before pyloric ligature, and in group 3, it was administered intraperitoneally 20 minutes before pyloric ligature. The number of ulcers in stomach was significantly low in group 2 (P = 0.01) and 3 (P = 0.037) in comparison with group 1. The level of ulcer was significantly decreased in group 2 (P = 0.047) with comparison to group 1. We conclude that, Echinops extract can exhibit potentially cytoprotective and anti-ulcer activity.

International Journal of Peptide Research and Therapeutics

Early-stage gastrointestinal (GI) carcinomas are amenable malignancies, however, due to late diag... more Early-stage gastrointestinal (GI) carcinomas are amenable malignancies, however, due to late diagnosis or lack of proper medication, alternative treatment necessitates new approaches such as dendritic cell (DC) therapy. Our previous microarray study indicated Lymphocyte antigen-6 (LY6E) as a commonly overexpressed biomarker in three lethal GI cancers, colon, gastric, and pancreatic. Therefore, we examined the antigenic potency of LY6E in stimulating DCs to elicit tumor-specific responses against human colorectal cancer (CRC) and gastric cancer (GC) cell lines HT-29 and AGS, respectively. LY6E peptide-pulsed DCs stimulated lymphocytes up to 55.9% in comparison with mature DCs (48.3%). Also, flow cytometry analysis of lymphocyte proliferation illustrated that the populations CD4+ and CD8+ were increased after treating by peptide-pulsed DCs (62.9% and 48.7% respectively). Furthermore, the cytotoxicity assay demonstrated that the 40:1 ratio of stimulated lymphocytes on AGS and HT29 cell lines was 65.1% and 66.2%, respectively. The research exposed that LY6E loaded DCs had substantial impact stimulation, proliferation, and lineage differentiation of lymphocytes. Besides, co-cultured of primed lymphocytes with AGS and HT29 cell lines exhibited cytotoxic activity. These data suggest LY6E as a potential candidate in developing DC therapy against CRC and AGS.

Iranian Journal of Allergy Asthma and Immunology, Jan 6, 2024

Inflammopharmacology, Mar 30, 2023

Chemokines belong to the family of cytokines with chemoattractant properties that regulate chemot... more Chemokines belong to the family of cytokines with chemoattractant properties that regulate chemotaxis and leukocyte migration, as well as the induction of angiogenesis and maintenance of hemostasis. Curcumin, the major component of the Curcuma longa rhizome, has various pharmacological actions, including anti-inflammatory, immune-regulatory, anti-oxidative, and lipid-modifying properties. Chemokines and chemokine receptors are influenced/modulated by curcumin. Thus, the current review focuses on the molecular mechanisms associated with curcumin's effects on chemoattractant cytokines, as well as putting into context the many studies that have reported curcumin-mediated regulatory effects on inflammatory conditions in the organs/systems of the body (e.g., the central nervous system, liver, and cardiovascular system). Curcumin's effects on viral and bacterial infections, cancer, and adverse pregnancy outcomes are also reviewed.

Phytotherapy Research, Jan 17, 2022

The present study conducted a placebo‐controlled clinical trial to evaluate the impact of nano‐cu... more The present study conducted a placebo‐controlled clinical trial to evaluate the impact of nano‐curcumin on the inflammatory cytokines in mild‐to‐moderate hospitalized COVID‐19 patients. A total of 60 COVID‐19 patients were randomly divided into nano‐curcumin and control groups, and then they received 240 mg/day nano‐curcumin for 7 days. The clinical manifestation and laboratory parameters in patients were recorded on days 0 and seven. Also, SYBR Green real‐time PCR and ELISA techniques were implicated in assessing the mRNA expression of IFN‐γ, IL‐1β, IL‐6, MCP‐1, and TNF‐α and the serum levels of IL‐1β, IL‐6, and TNF‐α inflammatory mediators, respectively. Although the clinical manifestations and laboratory parameters improved via the nano‐curcumin treatment, the mRNA expression of IFN‐γ (p = 0.006) and TNF‐α (p = 0.04) were significantly reduced. Besides, a considerable difference was observed between the nano‐curcumin and control groups in the expression of IFN‐γ (p = 0.001), IL‐1β (p = 0.0002), and IL‐6 (p = 0.008). In addition, there was a significant difference between the nano‐curcumin and control groups in the serum levels of IL‐1β (p = 0.042). The evidence demonstrated that nano‐curcumin could be implicated as a complementary medication to act as an antiinflammatory agent and inhibit inflammatory complications.

Immunobiology, May 1, 2023

International Immunopharmacology, Dec 1, 2022

International Immunopharmacology

Molecular and Cellular Probes, Aug 1, 2022

PubMed, Jun 1, 2019

Mesenchymal stem cells (MSCs) are well known due to their immunomodulatory effect, but the exact ... more Mesenchymal stem cells (MSCs) are well known due to their immunomodulatory effect, but the exact mechanisms have not been defined. Several studies demonstrated that the exerted immunoregulatory effect of these cells could be mediated by paracrine factors to illustrate, cytokines, chemokine, and among which, extracellular vesicles are one of them to play a crucial role. Moreover, it is assumed that extracellular vesicles are an essential player in intracellular communication by transferring their component. In this respect, the efficiency of conditioned media and exosomes was compared to illustrate a practical approach to cell-free based therapies. In the current study, we investigated the effect of both MSCs conditioned media (MSC-CM) and MSCs-derived exosomes on the expression of pro-inflammatory and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMCs). In this regard, isolated PBMCs were treated with MSC-CM and MSC-derived exosome as separated groups. Expression of inflammatory and anti-inflammatory markers was evaluated by Real-time PCR and ELISA. The immunoregulatory effect of MSC-CM on pro-inflammatory and anti-inflammatory genes, such as IL-12b, iNOS, EGR-2, IL-10 with an exception in case of IL-6 was more significant. Whereas in protein levels IL-10 showed the most substantial difference in exosome treated groups. It could be assumed that MSC-CM has more immunoregulatory impact on monocyte in contrast with exosomes.Taken together, by considering the recent approaches to cell-free therapy and the immunoregulatory impact of MSCs, yet relatively little is known about the efficacy of human-MSC-CM and secreted exosome compared with each other.

European Journal of Pharmacology, Sep 1, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Tissue & Cell, Aug 1, 2021

Tumor cells modulate immune responses by secreting exosomes. Tumor exosomes can affect the metabo... more Tumor cells modulate immune responses by secreting exosomes. Tumor exosomes can affect the metabolism of immune cells and increase immune inhibitory molecules such as programmed cell death protein 1 (PD-1). PD-1 inhibits the glycolysis pathway in immune cells. We investigated the role of tumor exosomes in how metabolic changes occur through the PD1-GLUT1-HK2 metabolic axisin peripheral blood mononuclear cells (PBMCs). The MDA-MB-231 cell line was cultured, serum samples from breast cancer patients were collected, and exosomes purified from serum samples and the MDA-MB-231 cell line. PBMCs were treated with purified exosomes for 72 h and, the expression of PD1-GLUT1-HK2 genes was measured by real-time PCR. Our study results showed relative expression of the HK2 gene in both groups treated with MDA-MB-231 cell line exosomes and serum exosomes of breast cancer patients was significantly increased compared to the control group (p < 0.0001). Also, the relative expression of the PD1 gene and GLUT1 gene showed a significant increase compared to the control group only in the group treated with MDA-MB-231 cell line exosomes (p < 0.0001). Therefore, Breast cancer exosomes increased the expression of key genes in the glycolysis pathway, increasing the glycolysis pathway in PBMCs. Increased expression of PD-1 could not prevent the expression of critical genes in the glycolysis pathway as in previous studies.

Inflammopharmacology

Chemokines belong to the family of cytokines with chemoattractant properties that regulate chemot... more Chemokines belong to the family of cytokines with chemoattractant properties that regulate chemotaxis and leukocyte migration, as well as the induction of angiogenesis and maintenance of hemostasis. Curcumin, the major component of the Curcuma longa rhizome, has various pharmacological actions, including anti-inflammatory, immune-regulatory, anti-oxidative, and lipid-modifying properties. Chemokines and chemokine receptors are influenced/modulated by curcumin. Thus, the current review focuses on the molecular mechanisms associated with curcumin's effects on chemoattractant cytokines, as well as putting into context the many studies that have reported curcumin-mediated regulatory effects on inflammatory conditions in the organs/systems of the body (e.g., the central nervous system, liver, and cardiovascular system). Curcumin's effects on viral and bacterial infections, cancer, and adverse pregnancy outcomes are also reviewed.

International Immunopharmacology

International Immunopharmacology, 2022

Nowadays, various strategies are considered to prime Dendritic cells (DCs) with tumor antigens. T... more Nowadays, various strategies are considered to prime Dendritic cells (DCs) with tumor antigens. The tumor cell-derived exosomes are recognized as one of the most efficient strategies for achieving this purpose. In this regard, MicroRNA 155 (miR-155) is employed as one of the most prominent miRNAs, which play substantial roles in DCs maturation and IL-12 production. This study investigates the tumor growth suppression and antitumor effects of DCs primed with miR-155-enriched exosome on the BALB/c murine model of colorectal cancer induced by CT-26 cell lines. Therefore, a holistic framework is proposed for the analysis procedure. In the first stage, miRNA-155 was electroporated into texosomes. In the second stage, bonemarrow-derived DCs were treated with miRNA-155 enriched texosomes. Then, antitumor properties of manipulated DC have been evaluated in the BALB/c mice model of colorectal cancer. After DC immunotherapy, several features have been assessed for each animal, including survival, body weight, tumor volume/size, histopathology, and serum cytokine levels. Also, flow cytometric evaluation has been performed for the spleen and the tumor tissue T-cell subsets. The findings demonstrated that the primed DCs could significantly increase IL-12p70 and IFN-γ in serum and accelerate the differentiation, proliferation, and cytotoxicity effects on the Th and CTL cells. Also, the treatment also increased the infiltration of Th and CTL cells into the tumor microenvironment while decreasing Tregs. This situation causes tumor growth control, and survival improvement. Therefore, DC immunotherapywith miR-155-enriched texosomes can be employed as a the desired approach for inducing antitumor immune responses, controlling tumor growth, and improving survival in mice with colorectal cancer. However, it is essential to perform more investigations to confirm the clinical application of this approach in humans and other types of tumors.

Molecular and Cellular Probes

Phytotherapy Research

The present study conducted a placebo‐controlled clinical trial to evaluate the impact of nano‐cu... more The present study conducted a placebo‐controlled clinical trial to evaluate the impact of nano‐curcumin on the inflammatory cytokines in mild‐to‐moderate hospitalized COVID‐19 patients. A total of 60 COVID‐19 patients were randomly divided into nano‐curcumin and control groups, and then they received 240 mg/day nano‐curcumin for 7 days. The clinical manifestation and laboratory parameters in patients were recorded on days 0 and seven. Also, SYBR Green real‐time PCR and ELISA techniques were implicated in assessing the mRNA expression of IFN‐γ, IL‐1β, IL‐6, MCP‐1, and TNF‐α and the serum levels of IL‐1β, IL‐6, and TNF‐α inflammatory mediators, respectively. Although the clinical manifestations and laboratory parameters improved via the nano‐curcumin treatment, the mRNA expression of IFN‐γ (p = 0.006) and TNF‐α (p = 0.04) were significantly reduced. Besides, a considerable difference was observed between the nano‐curcumin and control groups in the expression of IFN‐γ (p = 0.001), IL‐1β (p = 0.0002), and IL‐6 (p = 0.008). In addition, there was a significant difference between the nano‐curcumin and control groups in the serum levels of IL‐1β (p = 0.042). The evidence demonstrated that nano‐curcumin could be implicated as a complementary medication to act as an antiinflammatory agent and inhibit inflammatory complications.

Journal of Cellular Physiology, 2020

Extract of Echinops persicus is traditionally used for a long time in Iran for treatment of cough... more Extract of Echinops persicus is traditionally used for a long time in Iran for treatment of cough and constipation. This extract is produced by activity of bug (Situphilus spp.) on the plant. We documented its anti-tussive effect in rats in our previous study. The aim of this study was to assess the anti-ulcer effect of Echinops persicus in an animal model. In this study we evaluated anti-ulcer effect of Echinops persicus by Shay's method in rats. In 3 groups of rats, pylorus was ligatured under anesthesia. The rats were euthanized after 19 hours later and number and level of ulcer in stomach was measured. In group 2 the extract was orally administered 45 minutes before pyloric ligature, and in group 3, it was administered intraperitoneally 20 minutes before pyloric ligature. The number of ulcers in stomach was significantly low in group 2 (P = 0.01) and 3 (P = 0.037) in comparison with group 1. The level of ulcer was significantly decreased in group 2 (P = 0.047) with comparison to group 1. We conclude that, Echinops extract can exhibit potentially cytoprotective and anti-ulcer activity.

International Journal of Peptide Research and Therapeutics

Early-stage gastrointestinal (GI) carcinomas are amenable malignancies, however, due to late diag... more Early-stage gastrointestinal (GI) carcinomas are amenable malignancies, however, due to late diagnosis or lack of proper medication, alternative treatment necessitates new approaches such as dendritic cell (DC) therapy. Our previous microarray study indicated Lymphocyte antigen-6 (LY6E) as a commonly overexpressed biomarker in three lethal GI cancers, colon, gastric, and pancreatic. Therefore, we examined the antigenic potency of LY6E in stimulating DCs to elicit tumor-specific responses against human colorectal cancer (CRC) and gastric cancer (GC) cell lines HT-29 and AGS, respectively. LY6E peptide-pulsed DCs stimulated lymphocytes up to 55.9% in comparison with mature DCs (48.3%). Also, flow cytometry analysis of lymphocyte proliferation illustrated that the populations CD4+ and CD8+ were increased after treating by peptide-pulsed DCs (62.9% and 48.7% respectively). Furthermore, the cytotoxicity assay demonstrated that the 40:1 ratio of stimulated lymphocytes on AGS and HT29 cell lines was 65.1% and 66.2%, respectively. The research exposed that LY6E loaded DCs had substantial impact stimulation, proliferation, and lineage differentiation of lymphocytes. Besides, co-cultured of primed lymphocytes with AGS and HT29 cell lines exhibited cytotoxic activity. These data suggest LY6E as a potential candidate in developing DC therapy against CRC and AGS.