Ali Bahrami - Academia.edu (original) (raw)
Papers by Ali Bahrami
... This legacy information can be valuable, particularly in supplying process metrics and ad hoc... more ... This legacy information can be valuable, particularly in supplying process metrics and ad hoc business rules. 2.6 Usability and Acceptance ... 3 SYSTEM ARCHITECTURE ... Page 3. Enterprise Architecture for Business Process Simulation ...
We describe a prototype software tool suite for semantic information integration; it has the foll... more We describe a prototype software tool suite for semantic information integration; it has the following features. First, it can import local metadata as well as a domain ontology. Imported metadata is stored persistently in an ontological format. Second, it provides a semantic query facility that allows users to retrieve information across multiple data sources using the domain ontology directly. Third, it has a GUI for users to define mappings between the local metadata and the domain ontology. Fourth, it incorporates a novel mechanism to improve system reliability by dynamically adapting query execution upon detecting various types of environmental changes. In addition, this tool suite is compatible with W3C Semantic Web specifications such as RDF and OWL. It also uses the query engine of Commercial EII products for low level query processing.
In the coalition forces, users are increasingly challenged with the issues of information overloa... more In the coalition forces, users are increasingly challenged with the issues of information overload and correlation of information from heterogeneous sources. Users might need different pieces of information, ranging from information about a single building, to the resolution strategy of a global conflict. Sometimes, the time, location and past history of information access can also shape the information needs of users.
We describe a novel architecture for supporting mobile information access in the occasionally con... more We describe a novel architecture for supporting mobile information access in the occasionally connected computing environment. By utilizing user workflow, profiles, and environmental information, information from various sources published to the system is prioritized, cached and synchronized in the staging server, and eventually disseminated to mobile devices. Furthermore, workflow and user profile can help in predicting near future data needs and therefore can enable intelligent data charging
Biotechnology Letters, 2003
Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21(DE3) under the control of ... more Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21(DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was reached to 100 g dry wt l−1 under both constant (0.12 h−1) and variable (0.12–0.52 h−1) specific growth rates. In the variable specific growth rate fed-batch process, plasmid stability and specific yield of rhIFN-γ were greater than constant specific growth rate fed-batch process. The final specific yield and overall productivity of rhIFN-γ were 0.35±0.02 g rhIFN-γ g−1 dry cell wt and 0.9±0.05 g rhIFN-γ l−1 h−1 in the variable specific growth rate fed-batch process, respectively.
Biotechnology Letters, 2003
Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21(DE3) under the control of ... more Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21(DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was reached to 100 g dry wt l−1 under both constant (0.12 h−1) and variable (0.12–0.52 h−1) specific growth rates. In the variable specific growth rate fed-batch process, plasmid stability and specific yield of rhIFN-γ were greater than constant specific growth rate fed-batch process. The final specific yield and overall productivity of rhIFN-γ were 0.35±0.02 g rhIFN-γ g−1 dry cell wt and 0.9±0.05 g rhIFN-γ l−1 h−1 in the variable specific growth rate fed-batch process, respectively.
Journal of Industrial Microbiology & Biotechnology, 2004
A simple fed-batch process was carried out using constant and variable specific growth rates for ... more A simple fed-batch process was carried out using constant and variable specific growth rates for high-cell-density cultivation of Escherichia coli BL21 (DE3) expressing human interferon-γ(hIFN-γ). The feeding rate was adjusted to achieve an appropriate specific growth rate. The dissolved oxygen level was maintained at 20–30% of air saturation by control of airflow and stirrer speed and, where necessary, by enrichment of inlet air with pure oxygen. Glucose was the sole source of carbon and energy and was provided by following a simple exponential feeding rate. The final cell density in the fed-batch fermentation with constant and variable specific growth rate feeding strategies was ~100 g dry cell wt l−1 after 36 and 20 h, respectively. The final specific yield and overall productivity of recombinant hIFN-γ in the variable specific growth rate strategy were 0.35 g rHu-IFN-γ g−1 dry cell wt and 0.9 g rHu-IFN-γ l−1 h−1, respectively. A new chromatographic purification procedure involving anion exchange and cation exchange chromatographies was developed for purification of rHu-IFN-γ from inclusion bodies. The established purification process is reproducible and the total recovery of rHu-IFN-γ was ~30% (100 mg rHu-IFN-γ g−1 dry cell wt). The purity of the rHu-IFN-γ was determined using HPLC. Sterility, pyrogenicity, and DNA content tests were conducted to assure the absence of toxic materials and other components of E. coli in the final product. The final purified rHu-IFN-γ has a specific antiviral activity of ~2×107 IU/mg protein, as determined by viral cytopathic effect assay. These results certify the product for clinical purposes.
Biotechnology Letters, 2001
Anaerobic microbial biodegradation of dibenzothiophene (DBT) was studied using thermophilic bacte... more Anaerobic microbial biodegradation of dibenzothiophene (DBT) was studied using thermophilic bacteria obtained from crude oil. A mixed culture was obtained that degraded 98% of DBT at 0.5 mg ml−1 at 65 °C over 15 days both in the presence and in the absence of Methyl Viologen.
Data & Knowledge Engineering, 2000
The design of an information system (IS) has many consequences for the work of its users. We desc... more The design of an information system (IS) has many consequences for the work of its users. We describe a methodical structure that applies conceptual modeling for the iterative, coordinated design of user work and its supporting IS software. Our goal for this structure is to support ...
We present the case study of a complex, mixed-initiative scheduling system to illustrate Work-Cen... more We present the case study of a complex, mixed-initiative scheduling system to illustrate Work-Centered Design (WCD), a new approach for the design of information systems. WCD is based on theory of distributed cognition and extends established user-centered methods with abstract task modeling, using innovative techniques for work ontology and top-level algorithms to capture the logic of a human-computer interaction paradigm. WCD addresses a long-standing need for more effective methods of function allocation. The illustrating case study succeeded on a large, difficult problem for aircraft scheduling where prior expensive attempts failed. The new system, called Solver, reduces scheduling labor from 9 person-days a week to about 1 person-hour. These results were obtained from the first user test, demonstrating notable effectiveness of WCD. Further, the value of Solver's higher quality schedules is far-reaching. WCD extends HCI methods to fill an important need for technical problem-solving systems.
... This legacy information can be valuable, particularly in supplying process metrics and ad hoc... more ... This legacy information can be valuable, particularly in supplying process metrics and ad hoc business rules. 2.6 Usability and Acceptance ... 3 SYSTEM ARCHITECTURE ... Page 3. Enterprise Architecture for Business Process Simulation ...
We describe a prototype software tool suite for semantic information integration; it has the foll... more We describe a prototype software tool suite for semantic information integration; it has the following features. First, it can import local metadata as well as a domain ontology. Imported metadata is stored persistently in an ontological format. Second, it provides a semantic query facility that allows users to retrieve information across multiple data sources using the domain ontology directly. Third, it has a GUI for users to define mappings between the local metadata and the domain ontology. Fourth, it incorporates a novel mechanism to improve system reliability by dynamically adapting query execution upon detecting various types of environmental changes. In addition, this tool suite is compatible with W3C Semantic Web specifications such as RDF and OWL. It also uses the query engine of Commercial EII products for low level query processing.
In the coalition forces, users are increasingly challenged with the issues of information overloa... more In the coalition forces, users are increasingly challenged with the issues of information overload and correlation of information from heterogeneous sources. Users might need different pieces of information, ranging from information about a single building, to the resolution strategy of a global conflict. Sometimes, the time, location and past history of information access can also shape the information needs of users.
We describe a novel architecture for supporting mobile information access in the occasionally con... more We describe a novel architecture for supporting mobile information access in the occasionally connected computing environment. By utilizing user workflow, profiles, and environmental information, information from various sources published to the system is prioritized, cached and synchronized in the staging server, and eventually disseminated to mobile devices. Furthermore, workflow and user profile can help in predicting near future data needs and therefore can enable intelligent data charging
Biotechnology Letters, 2003
Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21(DE3) under the control of ... more Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21(DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was reached to 100 g dry wt l−1 under both constant (0.12 h−1) and variable (0.12–0.52 h−1) specific growth rates. In the variable specific growth rate fed-batch process, plasmid stability and specific yield of rhIFN-γ were greater than constant specific growth rate fed-batch process. The final specific yield and overall productivity of rhIFN-γ were 0.35±0.02 g rhIFN-γ g−1 dry cell wt and 0.9±0.05 g rhIFN-γ l−1 h−1 in the variable specific growth rate fed-batch process, respectively.
Biotechnology Letters, 2003
Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21(DE3) under the control of ... more Human interferon-gamma (hIFN-γ) was expressed in Escherichia coli BL21(DE3) under the control of the T7 promoter. Glucose was used as the sole source of carbon and energy with simple exponential feeding rate in fed-batch process. Cell density of recombinant E. coli was reached to 100 g dry wt l−1 under both constant (0.12 h−1) and variable (0.12–0.52 h−1) specific growth rates. In the variable specific growth rate fed-batch process, plasmid stability and specific yield of rhIFN-γ were greater than constant specific growth rate fed-batch process. The final specific yield and overall productivity of rhIFN-γ were 0.35±0.02 g rhIFN-γ g−1 dry cell wt and 0.9±0.05 g rhIFN-γ l−1 h−1 in the variable specific growth rate fed-batch process, respectively.
Journal of Industrial Microbiology & Biotechnology, 2004
A simple fed-batch process was carried out using constant and variable specific growth rates for ... more A simple fed-batch process was carried out using constant and variable specific growth rates for high-cell-density cultivation of Escherichia coli BL21 (DE3) expressing human interferon-γ(hIFN-γ). The feeding rate was adjusted to achieve an appropriate specific growth rate. The dissolved oxygen level was maintained at 20–30% of air saturation by control of airflow and stirrer speed and, where necessary, by enrichment of inlet air with pure oxygen. Glucose was the sole source of carbon and energy and was provided by following a simple exponential feeding rate. The final cell density in the fed-batch fermentation with constant and variable specific growth rate feeding strategies was ~100 g dry cell wt l−1 after 36 and 20 h, respectively. The final specific yield and overall productivity of recombinant hIFN-γ in the variable specific growth rate strategy were 0.35 g rHu-IFN-γ g−1 dry cell wt and 0.9 g rHu-IFN-γ l−1 h−1, respectively. A new chromatographic purification procedure involving anion exchange and cation exchange chromatographies was developed for purification of rHu-IFN-γ from inclusion bodies. The established purification process is reproducible and the total recovery of rHu-IFN-γ was ~30% (100 mg rHu-IFN-γ g−1 dry cell wt). The purity of the rHu-IFN-γ was determined using HPLC. Sterility, pyrogenicity, and DNA content tests were conducted to assure the absence of toxic materials and other components of E. coli in the final product. The final purified rHu-IFN-γ has a specific antiviral activity of ~2×107 IU/mg protein, as determined by viral cytopathic effect assay. These results certify the product for clinical purposes.
Biotechnology Letters, 2001
Anaerobic microbial biodegradation of dibenzothiophene (DBT) was studied using thermophilic bacte... more Anaerobic microbial biodegradation of dibenzothiophene (DBT) was studied using thermophilic bacteria obtained from crude oil. A mixed culture was obtained that degraded 98% of DBT at 0.5 mg ml−1 at 65 °C over 15 days both in the presence and in the absence of Methyl Viologen.
Data & Knowledge Engineering, 2000
The design of an information system (IS) has many consequences for the work of its users. We desc... more The design of an information system (IS) has many consequences for the work of its users. We describe a methodical structure that applies conceptual modeling for the iterative, coordinated design of user work and its supporting IS software. Our goal for this structure is to support ...
We present the case study of a complex, mixed-initiative scheduling system to illustrate Work-Cen... more We present the case study of a complex, mixed-initiative scheduling system to illustrate Work-Centered Design (WCD), a new approach for the design of information systems. WCD is based on theory of distributed cognition and extends established user-centered methods with abstract task modeling, using innovative techniques for work ontology and top-level algorithms to capture the logic of a human-computer interaction paradigm. WCD addresses a long-standing need for more effective methods of function allocation. The illustrating case study succeeded on a large, difficult problem for aircraft scheduling where prior expensive attempts failed. The new system, called Solver, reduces scheduling labor from 9 person-days a week to about 1 person-hour. These results were obtained from the first user test, demonstrating notable effectiveness of WCD. Further, the value of Solver's higher quality schedules is far-reaching. WCD extends HCI methods to fill an important need for technical problem-solving systems.