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Papers by Ali J.Mohammad
Analytica Chimica Acta, 1997
An lmmobllized enzyme electrode for the assay of oxalate, particularly In urlne, was constructed.... more An lmmobllized enzyme electrode for the assay of oxalate, particularly In urlne, was constructed. I t Is based on the lmmoblllzatlon of the enzyme oxalate oxidase on pig Intestine, mounted on the tip of an oxygen electrode. The oxygen partial pressure was amperometrlcally monitored, the current change being converted to a voltage through an adapter, whlch could then be monitored directly on a dlgttal voltmeter. The experimental parameters were all optimlzed, and callbration curves based on both the initial rate and the steady state were constructed. The enzyme electrode response to oxalate in urlne samples from 14 patlents was compared to that obtained by the establlshed spectrophotometric method. The proposed method exhibits high sensitivity and speclflclty to oxallc acid with almost no loss of relative activity due to interferences studied. There Is no sample pretreatment required and the method can be modlfled to be equally useful for the assay of oxallc acid in any blologlcal or nonblologlcal samples.
Analytica Chimica Acta, 1997
An lmmobllized enzyme electrode for the assay of oxalate, particularly In urlne, was constructed.... more An lmmobllized enzyme electrode for the assay of oxalate, particularly In urlne, was constructed. I t Is based on the lmmoblllzatlon of the enzyme oxalate oxidase on pig Intestine, mounted on the tip of an oxygen electrode. The oxygen partial pressure was amperometrlcally monitored, the current change being converted to a voltage through an adapter, whlch could then be monitored directly on a dlgttal voltmeter. The experimental parameters were all optimlzed, and callbration curves based on both the initial rate and the steady state were constructed. The enzyme electrode response to oxalate in urlne samples from 14 patlents was compared to that obtained by the establlshed spectrophotometric method. The proposed method exhibits high sensitivity and speclflclty to oxallc acid with almost no loss of relative activity due to interferences studied. There Is no sample pretreatment required and the method can be modlfled to be equally useful for the assay of oxallc acid in any blologlcal or nonblologlcal samples.