Ali Kilimcioğlu - Academia.edu (original) (raw)

Papers by Ali Kilimcioğlu

Research paper thumbnail of Temperature and Time Dependent Variations in Anti-Toxoplasma gondii Antibody Titrations in Serum Samples

Turkiye parazitolojii dergisi, 2021

OBJECTIVE This study aimed to determine the temperature- and time-dependent variations in the ant... more OBJECTIVE This study aimed to determine the temperature- and time-dependent variations in the anti-Toxoplasma gondii (T. gondii) antibody titres in serum samples collected from T. gondii-infected patients. METHODS During May 2017-February 2018, five serum samples (stored at different time periods and conditions) from pregnant or non-pregnant women aged ≥18 years who were infected with T. gondii and had applied to our Parasitology Department of Hafsa Sultan Hospital, Manisa Celal Bayar University, were investigated for the anti-T. gondii IgG antibody levels by enzyme linked fluorescent assay (ELFA). RESULTS The serum samples of five female volunteers who were infected with T. gondii that were stored at room temperature (20/25 °C), in a cargo package (+4/+8 °C), in a refrigerator (+4 °C), in a deep freezer (-16/-20 °C) and in an incubator (+37 °C) were tested at 0, 24, 48 and 72 hours after infection with the ELFA test. No statistically significant difference was observed in the anti-...

Research paper thumbnail of Comparison of culture media in the isolation and diagnosis of cutaneous leishmaniasis

African Journal of Microbiology Research, 2010

Leishmaniasis is a common and significant parasitic disease among the Middle East and Mediterrane... more Leishmaniasis is a common and significant parasitic disease among the Middle East and Mediterranean countries. Cutaneous and visceral forms of leishmaniasis are seen in Turkey. Direct microscopic examination of Giemsa-stained smears and inoculation into a culture medium are gold standards in diagnosis. In the present study, three diffferent culture media (pepton-yeast extract, brain heart and nutrient broth) were used in comparison with Novy-MacNeal-Nicolle and RPMI 1640 as control media to assess the effectiveness of new culture media both in diagnosis in patients and after isolation from the mice. In addition, smears prepared from the infectious material were stained with Giemsa and examined under a light microscope. Samples obtained from the lesions of cutaneous leishmaniasis produced on the sole of mice were inoculated into all culture media; growth were detected in pepton-yeast extract and nutrient broth media. Smears prepared from the lesions of these mice were stained with Gi...

Research paper thumbnail of Quercus infectoria Oliv. ve Achillea millefolium L. Ekstrelerinin Blastocystis spp. İzolatlarına in vitro Etkileri

Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of humans and ... more Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of humans and many animals. Despite it was firstly described almost 100 years ago; many subjects are still under debate about Blastocystis, including its life-cycle, pathogenic potential and treatment of infected individuals. Historically, local plant species have been used for therapeutic purposes by the local people of Anatolia. Here, hexane and methanol extracts of two local plants, Quercus infectoria (Fagaceae) and Achillea millefolium, which have been used against diarrhea in Anatolia, were examined for their in vitro efficacies against Blastocystis. LC50 and EC50 values of the plant extracts were determined by Brine Shrimp and Graphpad Prism 5® methods, respectively. The results showed that LC50 (500 μg/ml) and EC50 (198.8 μg/ml) concentrations of the methanol extract of A. millefolium were lowest compared to other extracts, its anti-Blastocystis activity was found to be comparable to metronidaz...

Research paper thumbnail of Trichomonas vaginalis’in Metronidazol, Ornidazol ve Proton Pompa İnhibitörleri Pantoprazol ve Esomeprazole Karşı İn Vitro Duyarlılığı

Mikrobiyoloji Bulteni, 2016

Kasım 2012, Kuşadası)'nde sunulmuş ve poster ikincilik ödülü almıştır. ÖZ Trikomoniyazın güncel t... more Kasım 2012, Kuşadası)'nde sunulmuş ve poster ikincilik ödülü almıştır. ÖZ Trikomoniyazın güncel tedavisinde 5-nitroimidazol türevleri kullanılmaktadır. Metronidazol, güvenilir, ucuz ve anaerobik mikroorganizmalara ve protozoonlara karşı çok etkili olmasına rağmen, T.vaginalis suşlarının metronidazole karşı geliştirdiği direnç, giderek artan bir sorun haline gelmektedir. Nitroimidazoller, azomisin (2-nitroimidazol) kimyasal yapısına sahip bileşikler olup, Streptomyces suşundan elde edilmektedir. Proton pompa inhibitörlerinin yapısındaki benzimidazol ise, antiprotozoal aktiviteye sahip diğer bileşiklerde de bulunan ortak bir yapıdır. Bu çalışmada, T.vaginalis'in metronidazol, ornidazol ve benzer kimyasal yapılara sahip yeni antiprotozoal ilaçlar olarak denenen proton pompa inhibitörleri pantoprazol ve esomeprazole karşı in vitro duyarlılığı araştırılmıştır. Bu amaçla, laboratuvarımızda önceden izole edilen ve kriyoprezervasyon yapılarak saklanan klinik bir T.vaginalis suşu kullanılmış ve ilaçların bu suşa karşı minimum inhibitör konsantrasyon (MİK) ve minimum letal konsantrasyon (MLK) değerleri, in vitro olarak 24 kuyucuklu hücre kültürü plaklarında dilüsyon yöntemiyle belirlenmiştir. Her bir kuyucuğunda triptikaz maya özütü maltoz besiyeri, at serumu ve antibiyotik (penisilin + streptomisin)

Research paper thumbnail of Bir üçüncü basamak hastanede Toxoplasma gondii IgG avidite test istem ve sonuçlarının değerlendirilmesi

Journal of Clinical and Experimental Investigations, 2014

Amac: Toksoplazmozis, Toxoplasma gondii (T.gondii) tarafindan olusturulan ve degisik klinik bulgu... more Amac: Toksoplazmozis, Toxoplasma gondii (T.gondii) tarafindan olusturulan ve degisik klinik bulgularla seyreden parazitik enfeksiyondur. Enfeksiyonun laboratuar tanisinda T. gondii\'ye karsi olusan IgG ve IgM antikorlarini belirleyen serolojik testler yaygin olarak kullanilmaktadir. Ozgul IgM antikorlarinin enfeksiyonun baslangic doneminde saptanamadigi, reaktivasyonlu bireylerde IgM antikorlarinin yukselmedigi durumlarda veya ozellikle gebelerde IgM pozitifliginde tanida enfeksiyonun baslangicini belirleyici IgG avidite testine gereksinim duyulmaktadir. Bu calismada; avidite test sonuclarinin degerlendirilmesi ve bu testin T. gondii enfeksiyonunda algoritmik yerinin belirlenmesi amaclanmistir. Yontemler: Bu calismada; seroloji laboratuarina 1 Ocak 2013-31 Aralik 2013 yilinda tum klinik ve servislerden istenen avidite test sonuclari degerlendirilmistir. Toplam 84 anti- T. gondii IgG avidite istegi yapilmistir. Calismaya alinan hastalarda anti- T. gondii IgG avidite kiti kullanilarak ELISA yontemi ile avidite degeri arastirilmistir. Anti- T. gondii IgG ve IgM antikor testleri ticari kit ile ELISA yontemiyle calisilmistir. Bulgular: 61 istek yuksek avidite %72.6, 13 istek dusuk avidite (%15.5), iki istek ara deger (%2.4), 8 istek gereksiz avidite istegi (avidite negatif) olarak degerlendirildi. Sonuclar: Toksoplazmozis supheli olgularda oncelikle anti-T. gondii IgG ve IgM testi yapilmasi, bu test sonuclarinin hastanin klinik durumuna ve/veya gebelerin hamileligin kacinci haftasinda olduguna gore degerlendirilerek yalniz uygun olgulardan IgG avidite test istemi yapilmasi gerektigi sonucuna varilmistir.

Research paper thumbnail of Türkiye’de İnsanlarda Kistik Ekinokokkoz

Mikrobiyoloji Bulteni

Kistik ekinokokkoz (KE) Türkiye'de oldukça yaygın olmasına rağmen, genellikle yıllarca semptom ve... more Kistik ekinokokkoz (KE) Türkiye'de oldukça yaygın olmasına rağmen, genellikle yıllarca semptom vermemesi ve zorunlu olduğu halde sıklıkla bildirilmemesi nedeniyle son derece ihmal edilmektedir. Enfeksiyonun bireysel ve kitlesel tanısında başta ultrasonografi (USG), ardından akciğer grafisi olmak üzere, radyolojik yöntemler önem taşır. Serolojik yöntemler, bilgisayarlı tomografi ve manyetik rezonans görüntüleme gibi ileri teknikler tanıyı doğrulamada ve cerrahi girişime hazırlık aşamasında yararlı olmaktadır. Türkiye'de insanlarda KE'nin prevalansına ilişkin verilerin çoğu hastane kayıtlarına, bildirilen olgulara ve serolojik yöntemlerin uygulandığı araştırmalara dayanmaktadır ve gerçeği yansıtmamaktadır. Seropozitif olguların büyük çoğunluğunda herhangi bir kist saptanamaması, serolojik yöntemlere dayalı araştırmaların değerini önemli ölçüde kısıtlamaktadır. KE'ye yönelik en değerli epidemiyolojik veriler taşınabilir USG kullanılarak yapılan kitle taramalarıyla elde edilir ve özellikle son 20 yılda serolojik yöntemlerin yerini USG almıştır. Türkiye'de USG kullanılarak yapılan kısıtlı çalışmaların ilkinde, Konya'nın bir köyünde, 20 yaş üzerindeki 190 kişinin 2 (%1.1)'sinde KE saptanmıştır. Manisa'da ilköğretim öğrencileri üzerinde gerçekleştirilen çalışmaların ilkinde, üç köyde USG, göğüs mikrofilmi ve serolojik testlerle araştırılan 630 öğrencinin 2 (%0.3)'sinde USG ile KE tanısı konmuştur. İkinci çalışmada, iki köyde sadece USG kullanılarak, 575 öğrencinin 3 (%0.5)'ünde hidatik kistler gözlenmiş; bu veriler KE'ye yönelik saha çalışmalarında USG'nin tek başına kullanılmasının daha kolay, hızlı ve yararlı olduğunu düşündürmüştür. Üçüncü çalışmada, dünyada ilk kez, il bazlı örneklem yöntemiyle, Manisa ilinin 37 farklı okulundaki 6093 öğrenci belirlenmiş ve USG ile 9 (%0.2) çocukta (ikisi önceden opere edilmiş) KE saptanmıştır. Bu modelin başka bölgelerde de uygulanabilmesi için yapılan çağrıya sadece Elazığ'dan yanıt gelmiş; burada seçilen 2500 öğrenci, USG ile incelendiğinde 6 olgu (biri önceden opere edilmiş) saptanmış, Elazığ ilinde prevalans %0.2 olarak belirlenmiştir. Aynı yıllarda, Van'da KE nedeniyle opere edilmiş 40 hasta ile aynı yaşam alanını paylaşan 102 olgunun 13 (%12.7)'ünde radyolojik olarak KE belirlenmiş; Aydın'da ise hayvancılık yapılan bir bölgedeki 209 olgudan 1 (%0.5)'inde USG ile KE saptanmıştır. Manisa'daki dördüncü araştırmada, 4275 üniversite öğrencisi USG ile araştırılmış, bu öğrencilerden 2034'ü "enzyme linked immunosorbent assay (ELISA)" ve "western blot (WB)" yöntemleri ile serolojik olarak da araştırılmıştır. WB yönteminin KE'de tarama testi olarak etkinliğinin dünyada ilk kez araştırıldığı çalışmada, USG ile altı yeni, üç opere edilmiş olgu saptanırken, gençlerde KE prevalansı %0.2 olarak belirlenmiştir. Bulgaristan, Romanya ve Türkiye'nin kırsal bölgelerinde USG ile yapılan araştırmalarda, Türkiye'nin altı Derleme/Review

Research paper thumbnail of Frequency of Intestinal Parasites Detected in the Laboratory of Medical Parasitology in Celal Bayar University Hafsa Sultan Hospital Between 2011 and 2015

Türk Mikrobiyoloji Cemiyeti Dergisi

Research paper thumbnail of Diagnosis of Blastocystis sp., Cryptosporidium sp., and Giardia intestinalis by Multiplex PCR: An Optimization Study

Türk Mikrobiyoloji Cemiyeti Dergisi

Objective: It was aimed to develop a new Multiplex Polymerase Chain Reaction (PCR) protocol with ... more Objective: It was aimed to develop a new Multiplex Polymerase Chain Reaction (PCR) protocol with isolates obtained from local patients for the diagnosis of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis, which can cause severe gastrointestinal system complaints especially in immunocompromised patients and children. Method: DNA isolation was performed with a commercial kit from three stool samples of different patients whose microscopic examination showed dense amounts of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis. First, a special PCR protocol has been developed for each protozoon. Then, the multiplex PCR protocol, in which these three protozoa can be diagnosed together, was optimized. Results: In the multiplex PCR protocol performed after DNA isolation, bands of 95 bp., 227 bp. and 258 bp. were obtained for Cryptosporidium sp., Blastocystis sp. and G. intestinalis, respectively. Conclusion: Blastocystis sp., Cryptosporidium sp. and Giardia intest...

Research paper thumbnail of Transmission of Dientamoeba fragilis: evaluation of the role of Enterobius vermicularis

Parasitology International, Mar 1, 2008

The role of Enterobius vermicularis in the transmission of Dientamoeba fragilis has been evaluate... more The role of Enterobius vermicularis in the transmission of Dientamoeba fragilis has been evaluated in two groups of patients admitted to the Parasitology Laboratory of Celal Bayar University: one group with E. vermicularis infection (n=187, Pinworm Group), and the other with D. fragilis infection (n=126, Dientamoeba Group). The presence of the other parasite, pinworm or Dientamoeba, was investigated with the microscopic examination of cellophane tape and stool samples for three consecutive days. In the Pinworm Group, 9.6% of the patients were found to be coinfected with D. fragilis, while 25.4% of the patients in the Dientamoeba Group were found to be coinfected with pinworms. The coincidence rates of D. fragilis and E. vermicularis, higher than the prevalence of each parasite in similar populations, suggest a common relation between these two parasites, possibly in entering the human body. E. vermicularis infection was found to be significantly more common in younger children (p<0.001), indicating that younger children may also be at higher risk for D. fragilis infection. These findings also raise the question of whether the unrelated symptoms of the pinworm infected patients such as abdominal pain and diarrhea may actually be due to overlooked Dientamoeba infections.

Research paper thumbnail of Health inequities: Lower socio-economic conditions and higher incidences of intestinal parasites

BMC Public Health

Intestinal parasitic infections affect child health and development and slow down growth, while r... more Intestinal parasitic infections affect child health and development and slow down growth, while reducing adults' productivity and work capacity. The aim of the present study was to determine and compare the incidences of intestinal parasitic infections and the socio-economic status of two near primary school children in Manisa, a western city of Turkey. A total of 352 children were involved a questionnaire study from a private school (Ulkem Primary School - UPS, 116 children) and a community-based school (Sehzadeler Primary School - SPS, 236 children). Of these, stool samples could be obtained from a total of 294 students; 97 (83.6%) from UPS, and 197 (83.5%) from SPS. The wet mount preparations of the stool samples were examined; samples were also fixed in polyvinyl alcohol and examined with modified formalin ethyl acetate sedimentation and trichrome staining techniques. Data were analyzed using SPSS for Windows version 10.0. The chi-squared test was used for the analytic asses...

Research paper thumbnail of Putative bronchopulmonary flagellated protozoa in immunosuppressed patients

BioMed research international, 2014

Flagellated protozoa that cause bronchopulmonary symptoms in humans are commonly neglected. These... more Flagellated protozoa that cause bronchopulmonary symptoms in humans are commonly neglected. These protozoal forms which were presumed to be "flagellated protozoa" have been previously identified in immunosuppressed patients in a number of studies, but have not been certainly classified so far. Since no human cases of bronchopulmonary flagellated protozoa were reported from Turkey, we aimed to investigate these putative protozoa in immunosuppressed patients who are particularly at risk of infectious diseases. Bronchoalveolar lavage fluid samples of 110 immunosuppressed adult patients who were admitted to the Department of Chest Diseases, Hafsa Sultan Hospital of Celal Bayar University, Manisa, Turkey, were examined in terms of parasites by light microscopy. Flagellated protozoal forms were detected in nine (8.2%) of 110 cases. Metronidazole (500 mg b.i.d. for 30 days) was given to all positive cases and a second bronchoscopy was performed at the end of the treatment, which ...

Research paper thumbnail of A mass screening survey of cystic echinococcosis by ultrasonography, Western blotting, and ELISA among university students in Manisa, Turkey

Acta tropica, 2013

Cystic echinococcosis (CE) is one of the most important zoonotic diseases in a wide geographic ar... more Cystic echinococcosis (CE) is one of the most important zoonotic diseases in a wide geographic area, including Turkey. In the present project, a total of 4275 students from Celal Bayar University, Manisa, Turkey, were screened by ultrasonography (US) and specific antibodies for CE were examined by Western blotting (WB) and ELISA in finger prick blood samples of 2034 of 4275 volunteered students. We aimed to report the apparent prevalence of CE based on different diagnostic procedures and to compare WB and ELISA with US in diagnosis of CE in a mass screening setting. Six new cases were diagnosed as CE by US during the survey. In addition to these cases, three students were also detected to have been previously operated and pathologically confirmed for hepatic CE. US revealed parenchymal changes in these cases in concordance with their operation history; so, the prevalence of CE by US was calculated as 0.21% (9/4275) (95%CI, 0.11-0.39%) among university students in Manisa. Bands were ...

Research paper thumbnail of In vitro susceptibility of Trichomonas vaginalis strains to metronidazole--a Nigerian experience

Genitourinary medicine, 1993

We determined the susceptibilities of five strains of Trichomonas vaginalis, one of which was met... more We determined the susceptibilities of five strains of Trichomonas vaginalis, one of which was metronidazole resistant, to 50 antimicrobial agents. For the metronidazole-susceptible strains, the most active agents were metronidazole, tinidazole, mebendazole, furazolidone, and anisomycin. Against the resistant strain mebendazole, furazolidone, and anisomycin were the most active. Antifungal agents, beta-lactams, macrolides, aminoglycosides, and folic acid antagonists were ineffective against all strains.

Research paper thumbnail of A discrepancy between cystic echinococcosis confirmed by ultrasound and seropositivity in Turkish children

Acta Tropica

In three randomly selected villages of Manisa, Turkey, 630 primary school children were examined ... more In three randomly selected villages of Manisa, Turkey, 630 primary school children were examined for cystic echinococcosis (CE) by a portable ultrasound scanner (US) and chest microfilm. Sera samples of 483 (76.7%) children were tested for anti-E. granulosus antibodies by ELISA and indirect hemagglutination (IHA) tests. Hepatic CE were detected in two cases (0.3%) by US, while 43 (8.9%) and 49 (10.1%) cases were found to be positive for CE by ELISA and IHA, respectively. The high seropositivity levels could have been attributed to extra-abdominal or abortive Echinococcus infections, but probably most of them were false-positives. Due to the discrepancy in results, US should be selected as the primary test in field studies and serologic tests should be performed in all cases with suspected lesions. We could not find any reported community based study on CE in Turkey, in which US was applied; but our results suggest that extensive epidemiological studies are required.

Research paper thumbnail of In vitro Efficacy of Quercus infectoria Oliv. and Achillea millefolium L. Extracts Against Blastocystis spp. Isolates

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2013

ABSTRACT Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of hu... more ABSTRACT Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of humans and many animals. Despite it was firstly described almost 100 years ago; many subjects are still under debate about Blastocystis, including its life-cycle, pathogenic potential and treatment of infected individuals. Historically, local plant species have been used for therapeutic purposes by the local people of Anatolia. Here, hexane and methanol extracts of two local plants, Quercus infectoria (Fagaceae) and Achillea millefolium, which have been used against diarrhea in Anatolia, were examined for their in vitro efficacies against Blastocystis. LC50 and EC50 values of the plant extracts were determined by Brine Shrimp and Graphpad Prism 5® methods, respectively. The results showed that LC50 (500 μg/ml) and EC50 (198.8 μg/ml) concentrations of the methanol extract of A. millefolium were lowest compared to other extracts, its anti-Blastocystis activity was found to be comparable to metronidazole and it showed no cytotoxic activity. These initial results suggest that the methanol extract of A. millefolium may be a novel option for the treatment of Blastocystis infections in humans in future, if confirmed by further, larger-scale studies.

Research paper thumbnail of Incidence of Intestinal Parasites Detected in the Department of Parasitology in Celal Bayar University Hospital between 2006 and 2010

Turkish Journal of Parasitology, 2012

Selofan bant örneği alınan 5952 hastanın 253'ünde (%4.25) Enterobius vermicularis saptanmıştır. P... more Selofan bant örneği alınan 5952 hastanın 253'ünde (%4.25) Enterobius vermicularis saptanmıştır. Pozitif olguların 158'inde (%6.76) iki veya daha fazla parazit birlikte görülmüştür. Parazit saptanan kişilerin yaş ortalaması 21.9 olarak bulunmuştur. Erkeklerde parazit görülme oranı %13.6 iken kadınlarda %10.7 saptanmıştır (p<0.001). Parazit saptanan olgular yerleşim yerlerine göre değerlendirildiğinde en yüksek oranın kırsal bölgelerde yaşayanlarda (%23.8) olduğu görülmüştür (p=0.006). Sonuç: İlimizde bağırsak parazitleri arasında en sık Blastocystis spp., E. vermicularis ve G. intestinalis görülürken, son yıllarda alt yapı çalışmalarındaki ilerlemelere rağmen bağırsak parazitlerinin halen önemini koruduğu görülmüştür.

Research paper thumbnail of Evaluation of Serological Results of Patients with Suspected Toxoplasmosis Admitted to the Medical Parasitology Laboratory of Celal Bayar University Hospital between 2006-2010

Turkish Journal of Parasitology, 2012

IgG ve IgM antikorları araştırılmıştır. IgG ve IgM birlikte pozitif bulunduğu olgularda ayrıca Ig... more IgG ve IgM antikorları araştırılmıştır. IgG ve IgM birlikte pozitif bulunduğu olgularda ayrıca IgG avidite yöntemi uygulanmıştır. Bulgular: Araştırmamızda 2815 hastanın 657'sinde (%23.3) yalnız IgG pozitifliği, 4'ünde (%0.1) yalnız IgM pozitifliği saptanırken, 6 (%0.2) hastada IgG ve IgM birlikte pozitif saptanmıştır. Anti-T. gondii IgG seropozitifliği en çok çiğ et tüketen hastalarda [%21.2 (n=139)] görülmüştür. 15-49 yaş grubunda IgG seroprevalansı 0-14 yaş grubundan istatistiksel olarak yüksek bulunmuştur (p<0.05). Serolojik sonuçları beş yıllık periyoda göre değerlendirdiğimizde 2010 yılında anti-T. gondii seroprevalansının düştüğü görülmüş, bu oran istatistiksel olarak anlamlı bulunmuştur (p<0.001). Sonuç: Toxoplasma enfeksiyonunda çiğ et yeme alışkanlığının en önemli risk faktörü olduğu saptanmıştır. Beş yıllık serolojik verileri değerlendirdiğimizde son yıllarda anti-T. gondii IgG antikorlarında bir azalma saptanmış, bununla birlikte Manisa'da toxoplasmosisin halen önemli bir halk sağlığı sorunu olduğu görülmüştür.

Research paper thumbnail of Putative Bronchopulmonary Flagellated Protozoa in Immunosuppressed Patients

BioMed Research International, 2014

Flagellated protozoa that cause bronchopulmonary symptoms in humans are commonly neglected. These... more Flagellated protozoa that cause bronchopulmonary symptoms in humans are commonly neglected. These protozoal forms which were presumed to be “flagellated protozoa” have been previously identified in immunosuppressed patients in a number of studies, but have not been certainly classified so far. Since no human cases of bronchopulmonary flagellated protozoa were reported from Turkey, we aimed to investigate these putative protozoa in immunosuppressed patients who are particularly at risk of infectious diseases. Bronchoalveolar lavage fluid samples of 110 immunosuppressed adult patients who were admitted to the Department of Chest Diseases, Hafsa Sultan Hospital of Celal Bayar University, Manisa, Turkey, were examined in terms of parasites by light microscopy. Flagellated protozoal forms were detected in nine (8.2%) of 110 cases. Metronidazole (500 mgb.i.d.for 30 days) was given to all positive cases and a second bronchoscopy was performed at the end of the treatment, which revealed no ...

Research paper thumbnail of Effect of trimethoprim-sulfamethaxazole in blastocystis hominis infection

The American Journal of Gastroenterology, 1999

Blastocystis hominis (B. hominis) is a common intestinal parasite that has long been considered n... more Blastocystis hominis (B. hominis) is a common intestinal parasite that has long been considered nonpathogenic. Recently there have been many reports supporting a role for the organism as a potential pathogen. We performed a study to examine the pathogenicity of B. hominis and the effect of trimethoprim-sulfamethaxazole (TMP-SMX) on this organism. Stool samples of patients, who came to the Department of Parasitology, Faculty of Medicine, Celal Bayar University, were examined by direct wet-mount, trichrome staining, formalin-ethyl acetate concentration, and Kinyoun acid fast techniques for intestinal parasites, and bacteriological stool cultures were performed. Fifty-three symptomatic patients (38 children and 15 adults) with two consequent stool samples positive for abundant B. hominis (five or more organisms per x400 field) and negative for other parasitic and bacterial pathogens were treated with TMP-SMX for 7 days, children 6 mg/kg TMP, 30 mg/kg SMX, and adults 320 mg TMP, 1600 mg SMX, daily. On the seventh day, at the end of treatment, stool samples of all patients were examined by same methods, and clinical symptoms were again evaluated. B. hominis was eradicated in 36 of 38 (94.7%) children, and 14 of 15 (93.3%) adults. Clinical symptoms disappeared in 39 (73.6%), decreased in 10 (18.9%), and no change was observed in one (1.9%) patient, whereas symptoms persisted in all three (5.7%) patients in whom B. hominis could not be eradicated. Mean number of stools per day was significantly decreased from 4.3 to 1.2 in the 33 children (p &lt; 0.001), and decreased from 3.5 to 1.0 in the four adults (p = 0.06) with diarrhea. These results suggested that B. hominis may be pathogenic, especially when it is present in large numbers, and TMP-SMX is highly effective against this organism. Although there are some anecdotal reports, to our knowledge this is the first study examining the effect of TMP-SMX on B. hominis in humans.

Research paper thumbnail of Transmission of Dientamoeba fragilis: Evaluation of the role of Enterobius vermicularis

Parasitology International, 2008

The role of Enterobius vermicularis in the transmission of Dientamoeba fragilis has been evaluate... more The role of Enterobius vermicularis in the transmission of Dientamoeba fragilis has been evaluated in two groups of patients admitted to the Parasitology Laboratory of Celal Bayar University: one group with E. vermicularis infection (n=187, Pinworm Group), and the other with D. fragilis infection (n=126, Dientamoeba Group). The presence of the other parasite, pinworm or Dientamoeba, was investigated with the microscopic examination of cellophane tape and stool samples for three consecutive days. In the Pinworm Group, 9.6% of the patients were found to be coinfected with D. fragilis, while 25.4% of the patients in the Dientamoeba Group were found to be coinfected with pinworms. The coincidence rates of D. fragilis and E. vermicularis, higher than the prevalence of each parasite in similar populations, suggest a common relation between these two parasites, possibly in entering the human body. E. vermicularis infection was found to be significantly more common in younger children (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001), indicating that younger children may also be at higher risk for D. fragilis infection. These findings also raise the question of whether the unrelated symptoms of the pinworm infected patients such as abdominal pain and diarrhea may actually be due to overlooked Dientamoeba infections.

Research paper thumbnail of Temperature and Time Dependent Variations in Anti-Toxoplasma gondii Antibody Titrations in Serum Samples

Turkiye parazitolojii dergisi, 2021

OBJECTIVE This study aimed to determine the temperature- and time-dependent variations in the ant... more OBJECTIVE This study aimed to determine the temperature- and time-dependent variations in the anti-Toxoplasma gondii (T. gondii) antibody titres in serum samples collected from T. gondii-infected patients. METHODS During May 2017-February 2018, five serum samples (stored at different time periods and conditions) from pregnant or non-pregnant women aged ≥18 years who were infected with T. gondii and had applied to our Parasitology Department of Hafsa Sultan Hospital, Manisa Celal Bayar University, were investigated for the anti-T. gondii IgG antibody levels by enzyme linked fluorescent assay (ELFA). RESULTS The serum samples of five female volunteers who were infected with T. gondii that were stored at room temperature (20/25 °C), in a cargo package (+4/+8 °C), in a refrigerator (+4 °C), in a deep freezer (-16/-20 °C) and in an incubator (+37 °C) were tested at 0, 24, 48 and 72 hours after infection with the ELFA test. No statistically significant difference was observed in the anti-...

Research paper thumbnail of Comparison of culture media in the isolation and diagnosis of cutaneous leishmaniasis

African Journal of Microbiology Research, 2010

Leishmaniasis is a common and significant parasitic disease among the Middle East and Mediterrane... more Leishmaniasis is a common and significant parasitic disease among the Middle East and Mediterranean countries. Cutaneous and visceral forms of leishmaniasis are seen in Turkey. Direct microscopic examination of Giemsa-stained smears and inoculation into a culture medium are gold standards in diagnosis. In the present study, three diffferent culture media (pepton-yeast extract, brain heart and nutrient broth) were used in comparison with Novy-MacNeal-Nicolle and RPMI 1640 as control media to assess the effectiveness of new culture media both in diagnosis in patients and after isolation from the mice. In addition, smears prepared from the infectious material were stained with Giemsa and examined under a light microscope. Samples obtained from the lesions of cutaneous leishmaniasis produced on the sole of mice were inoculated into all culture media; growth were detected in pepton-yeast extract and nutrient broth media. Smears prepared from the lesions of these mice were stained with Gi...

Research paper thumbnail of Quercus infectoria Oliv. ve Achillea millefolium L. Ekstrelerinin Blastocystis spp. İzolatlarına in vitro Etkileri

Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of humans and ... more Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of humans and many animals. Despite it was firstly described almost 100 years ago; many subjects are still under debate about Blastocystis, including its life-cycle, pathogenic potential and treatment of infected individuals. Historically, local plant species have been used for therapeutic purposes by the local people of Anatolia. Here, hexane and methanol extracts of two local plants, Quercus infectoria (Fagaceae) and Achillea millefolium, which have been used against diarrhea in Anatolia, were examined for their in vitro efficacies against Blastocystis. LC50 and EC50 values of the plant extracts were determined by Brine Shrimp and Graphpad Prism 5® methods, respectively. The results showed that LC50 (500 μg/ml) and EC50 (198.8 μg/ml) concentrations of the methanol extract of A. millefolium were lowest compared to other extracts, its anti-Blastocystis activity was found to be comparable to metronidaz...

Research paper thumbnail of Trichomonas vaginalis’in Metronidazol, Ornidazol ve Proton Pompa İnhibitörleri Pantoprazol ve Esomeprazole Karşı İn Vitro Duyarlılığı

Mikrobiyoloji Bulteni, 2016

Kasım 2012, Kuşadası)'nde sunulmuş ve poster ikincilik ödülü almıştır. ÖZ Trikomoniyazın güncel t... more Kasım 2012, Kuşadası)'nde sunulmuş ve poster ikincilik ödülü almıştır. ÖZ Trikomoniyazın güncel tedavisinde 5-nitroimidazol türevleri kullanılmaktadır. Metronidazol, güvenilir, ucuz ve anaerobik mikroorganizmalara ve protozoonlara karşı çok etkili olmasına rağmen, T.vaginalis suşlarının metronidazole karşı geliştirdiği direnç, giderek artan bir sorun haline gelmektedir. Nitroimidazoller, azomisin (2-nitroimidazol) kimyasal yapısına sahip bileşikler olup, Streptomyces suşundan elde edilmektedir. Proton pompa inhibitörlerinin yapısındaki benzimidazol ise, antiprotozoal aktiviteye sahip diğer bileşiklerde de bulunan ortak bir yapıdır. Bu çalışmada, T.vaginalis'in metronidazol, ornidazol ve benzer kimyasal yapılara sahip yeni antiprotozoal ilaçlar olarak denenen proton pompa inhibitörleri pantoprazol ve esomeprazole karşı in vitro duyarlılığı araştırılmıştır. Bu amaçla, laboratuvarımızda önceden izole edilen ve kriyoprezervasyon yapılarak saklanan klinik bir T.vaginalis suşu kullanılmış ve ilaçların bu suşa karşı minimum inhibitör konsantrasyon (MİK) ve minimum letal konsantrasyon (MLK) değerleri, in vitro olarak 24 kuyucuklu hücre kültürü plaklarında dilüsyon yöntemiyle belirlenmiştir. Her bir kuyucuğunda triptikaz maya özütü maltoz besiyeri, at serumu ve antibiyotik (penisilin + streptomisin)

Research paper thumbnail of Bir üçüncü basamak hastanede Toxoplasma gondii IgG avidite test istem ve sonuçlarının değerlendirilmesi

Journal of Clinical and Experimental Investigations, 2014

Amac: Toksoplazmozis, Toxoplasma gondii (T.gondii) tarafindan olusturulan ve degisik klinik bulgu... more Amac: Toksoplazmozis, Toxoplasma gondii (T.gondii) tarafindan olusturulan ve degisik klinik bulgularla seyreden parazitik enfeksiyondur. Enfeksiyonun laboratuar tanisinda T. gondii\'ye karsi olusan IgG ve IgM antikorlarini belirleyen serolojik testler yaygin olarak kullanilmaktadir. Ozgul IgM antikorlarinin enfeksiyonun baslangic doneminde saptanamadigi, reaktivasyonlu bireylerde IgM antikorlarinin yukselmedigi durumlarda veya ozellikle gebelerde IgM pozitifliginde tanida enfeksiyonun baslangicini belirleyici IgG avidite testine gereksinim duyulmaktadir. Bu calismada; avidite test sonuclarinin degerlendirilmesi ve bu testin T. gondii enfeksiyonunda algoritmik yerinin belirlenmesi amaclanmistir. Yontemler: Bu calismada; seroloji laboratuarina 1 Ocak 2013-31 Aralik 2013 yilinda tum klinik ve servislerden istenen avidite test sonuclari degerlendirilmistir. Toplam 84 anti- T. gondii IgG avidite istegi yapilmistir. Calismaya alinan hastalarda anti- T. gondii IgG avidite kiti kullanilarak ELISA yontemi ile avidite degeri arastirilmistir. Anti- T. gondii IgG ve IgM antikor testleri ticari kit ile ELISA yontemiyle calisilmistir. Bulgular: 61 istek yuksek avidite %72.6, 13 istek dusuk avidite (%15.5), iki istek ara deger (%2.4), 8 istek gereksiz avidite istegi (avidite negatif) olarak degerlendirildi. Sonuclar: Toksoplazmozis supheli olgularda oncelikle anti-T. gondii IgG ve IgM testi yapilmasi, bu test sonuclarinin hastanin klinik durumuna ve/veya gebelerin hamileligin kacinci haftasinda olduguna gore degerlendirilerek yalniz uygun olgulardan IgG avidite test istemi yapilmasi gerektigi sonucuna varilmistir.

Research paper thumbnail of Türkiye’de İnsanlarda Kistik Ekinokokkoz

Mikrobiyoloji Bulteni

Kistik ekinokokkoz (KE) Türkiye'de oldukça yaygın olmasına rağmen, genellikle yıllarca semptom ve... more Kistik ekinokokkoz (KE) Türkiye'de oldukça yaygın olmasına rağmen, genellikle yıllarca semptom vermemesi ve zorunlu olduğu halde sıklıkla bildirilmemesi nedeniyle son derece ihmal edilmektedir. Enfeksiyonun bireysel ve kitlesel tanısında başta ultrasonografi (USG), ardından akciğer grafisi olmak üzere, radyolojik yöntemler önem taşır. Serolojik yöntemler, bilgisayarlı tomografi ve manyetik rezonans görüntüleme gibi ileri teknikler tanıyı doğrulamada ve cerrahi girişime hazırlık aşamasında yararlı olmaktadır. Türkiye'de insanlarda KE'nin prevalansına ilişkin verilerin çoğu hastane kayıtlarına, bildirilen olgulara ve serolojik yöntemlerin uygulandığı araştırmalara dayanmaktadır ve gerçeği yansıtmamaktadır. Seropozitif olguların büyük çoğunluğunda herhangi bir kist saptanamaması, serolojik yöntemlere dayalı araştırmaların değerini önemli ölçüde kısıtlamaktadır. KE'ye yönelik en değerli epidemiyolojik veriler taşınabilir USG kullanılarak yapılan kitle taramalarıyla elde edilir ve özellikle son 20 yılda serolojik yöntemlerin yerini USG almıştır. Türkiye'de USG kullanılarak yapılan kısıtlı çalışmaların ilkinde, Konya'nın bir köyünde, 20 yaş üzerindeki 190 kişinin 2 (%1.1)'sinde KE saptanmıştır. Manisa'da ilköğretim öğrencileri üzerinde gerçekleştirilen çalışmaların ilkinde, üç köyde USG, göğüs mikrofilmi ve serolojik testlerle araştırılan 630 öğrencinin 2 (%0.3)'sinde USG ile KE tanısı konmuştur. İkinci çalışmada, iki köyde sadece USG kullanılarak, 575 öğrencinin 3 (%0.5)'ünde hidatik kistler gözlenmiş; bu veriler KE'ye yönelik saha çalışmalarında USG'nin tek başına kullanılmasının daha kolay, hızlı ve yararlı olduğunu düşündürmüştür. Üçüncü çalışmada, dünyada ilk kez, il bazlı örneklem yöntemiyle, Manisa ilinin 37 farklı okulundaki 6093 öğrenci belirlenmiş ve USG ile 9 (%0.2) çocukta (ikisi önceden opere edilmiş) KE saptanmıştır. Bu modelin başka bölgelerde de uygulanabilmesi için yapılan çağrıya sadece Elazığ'dan yanıt gelmiş; burada seçilen 2500 öğrenci, USG ile incelendiğinde 6 olgu (biri önceden opere edilmiş) saptanmış, Elazığ ilinde prevalans %0.2 olarak belirlenmiştir. Aynı yıllarda, Van'da KE nedeniyle opere edilmiş 40 hasta ile aynı yaşam alanını paylaşan 102 olgunun 13 (%12.7)'ünde radyolojik olarak KE belirlenmiş; Aydın'da ise hayvancılık yapılan bir bölgedeki 209 olgudan 1 (%0.5)'inde USG ile KE saptanmıştır. Manisa'daki dördüncü araştırmada, 4275 üniversite öğrencisi USG ile araştırılmış, bu öğrencilerden 2034'ü "enzyme linked immunosorbent assay (ELISA)" ve "western blot (WB)" yöntemleri ile serolojik olarak da araştırılmıştır. WB yönteminin KE'de tarama testi olarak etkinliğinin dünyada ilk kez araştırıldığı çalışmada, USG ile altı yeni, üç opere edilmiş olgu saptanırken, gençlerde KE prevalansı %0.2 olarak belirlenmiştir. Bulgaristan, Romanya ve Türkiye'nin kırsal bölgelerinde USG ile yapılan araştırmalarda, Türkiye'nin altı Derleme/Review

Research paper thumbnail of Frequency of Intestinal Parasites Detected in the Laboratory of Medical Parasitology in Celal Bayar University Hafsa Sultan Hospital Between 2011 and 2015

Türk Mikrobiyoloji Cemiyeti Dergisi

Research paper thumbnail of Diagnosis of Blastocystis sp., Cryptosporidium sp., and Giardia intestinalis by Multiplex PCR: An Optimization Study

Türk Mikrobiyoloji Cemiyeti Dergisi

Objective: It was aimed to develop a new Multiplex Polymerase Chain Reaction (PCR) protocol with ... more Objective: It was aimed to develop a new Multiplex Polymerase Chain Reaction (PCR) protocol with isolates obtained from local patients for the diagnosis of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis, which can cause severe gastrointestinal system complaints especially in immunocompromised patients and children. Method: DNA isolation was performed with a commercial kit from three stool samples of different patients whose microscopic examination showed dense amounts of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis. First, a special PCR protocol has been developed for each protozoon. Then, the multiplex PCR protocol, in which these three protozoa can be diagnosed together, was optimized. Results: In the multiplex PCR protocol performed after DNA isolation, bands of 95 bp., 227 bp. and 258 bp. were obtained for Cryptosporidium sp., Blastocystis sp. and G. intestinalis, respectively. Conclusion: Blastocystis sp., Cryptosporidium sp. and Giardia intest...

Research paper thumbnail of Transmission of Dientamoeba fragilis: evaluation of the role of Enterobius vermicularis

Parasitology International, Mar 1, 2008

The role of Enterobius vermicularis in the transmission of Dientamoeba fragilis has been evaluate... more The role of Enterobius vermicularis in the transmission of Dientamoeba fragilis has been evaluated in two groups of patients admitted to the Parasitology Laboratory of Celal Bayar University: one group with E. vermicularis infection (n=187, Pinworm Group), and the other with D. fragilis infection (n=126, Dientamoeba Group). The presence of the other parasite, pinworm or Dientamoeba, was investigated with the microscopic examination of cellophane tape and stool samples for three consecutive days. In the Pinworm Group, 9.6% of the patients were found to be coinfected with D. fragilis, while 25.4% of the patients in the Dientamoeba Group were found to be coinfected with pinworms. The coincidence rates of D. fragilis and E. vermicularis, higher than the prevalence of each parasite in similar populations, suggest a common relation between these two parasites, possibly in entering the human body. E. vermicularis infection was found to be significantly more common in younger children (p<0.001), indicating that younger children may also be at higher risk for D. fragilis infection. These findings also raise the question of whether the unrelated symptoms of the pinworm infected patients such as abdominal pain and diarrhea may actually be due to overlooked Dientamoeba infections.

Research paper thumbnail of Health inequities: Lower socio-economic conditions and higher incidences of intestinal parasites

BMC Public Health

Intestinal parasitic infections affect child health and development and slow down growth, while r... more Intestinal parasitic infections affect child health and development and slow down growth, while reducing adults' productivity and work capacity. The aim of the present study was to determine and compare the incidences of intestinal parasitic infections and the socio-economic status of two near primary school children in Manisa, a western city of Turkey. A total of 352 children were involved a questionnaire study from a private school (Ulkem Primary School - UPS, 116 children) and a community-based school (Sehzadeler Primary School - SPS, 236 children). Of these, stool samples could be obtained from a total of 294 students; 97 (83.6%) from UPS, and 197 (83.5%) from SPS. The wet mount preparations of the stool samples were examined; samples were also fixed in polyvinyl alcohol and examined with modified formalin ethyl acetate sedimentation and trichrome staining techniques. Data were analyzed using SPSS for Windows version 10.0. The chi-squared test was used for the analytic asses...

Research paper thumbnail of Putative bronchopulmonary flagellated protozoa in immunosuppressed patients

BioMed research international, 2014

Flagellated protozoa that cause bronchopulmonary symptoms in humans are commonly neglected. These... more Flagellated protozoa that cause bronchopulmonary symptoms in humans are commonly neglected. These protozoal forms which were presumed to be "flagellated protozoa" have been previously identified in immunosuppressed patients in a number of studies, but have not been certainly classified so far. Since no human cases of bronchopulmonary flagellated protozoa were reported from Turkey, we aimed to investigate these putative protozoa in immunosuppressed patients who are particularly at risk of infectious diseases. Bronchoalveolar lavage fluid samples of 110 immunosuppressed adult patients who were admitted to the Department of Chest Diseases, Hafsa Sultan Hospital of Celal Bayar University, Manisa, Turkey, were examined in terms of parasites by light microscopy. Flagellated protozoal forms were detected in nine (8.2%) of 110 cases. Metronidazole (500 mg b.i.d. for 30 days) was given to all positive cases and a second bronchoscopy was performed at the end of the treatment, which ...

Research paper thumbnail of A mass screening survey of cystic echinococcosis by ultrasonography, Western blotting, and ELISA among university students in Manisa, Turkey

Acta tropica, 2013

Cystic echinococcosis (CE) is one of the most important zoonotic diseases in a wide geographic ar... more Cystic echinococcosis (CE) is one of the most important zoonotic diseases in a wide geographic area, including Turkey. In the present project, a total of 4275 students from Celal Bayar University, Manisa, Turkey, were screened by ultrasonography (US) and specific antibodies for CE were examined by Western blotting (WB) and ELISA in finger prick blood samples of 2034 of 4275 volunteered students. We aimed to report the apparent prevalence of CE based on different diagnostic procedures and to compare WB and ELISA with US in diagnosis of CE in a mass screening setting. Six new cases were diagnosed as CE by US during the survey. In addition to these cases, three students were also detected to have been previously operated and pathologically confirmed for hepatic CE. US revealed parenchymal changes in these cases in concordance with their operation history; so, the prevalence of CE by US was calculated as 0.21% (9/4275) (95%CI, 0.11-0.39%) among university students in Manisa. Bands were ...

Research paper thumbnail of In vitro susceptibility of Trichomonas vaginalis strains to metronidazole--a Nigerian experience

Genitourinary medicine, 1993

We determined the susceptibilities of five strains of Trichomonas vaginalis, one of which was met... more We determined the susceptibilities of five strains of Trichomonas vaginalis, one of which was metronidazole resistant, to 50 antimicrobial agents. For the metronidazole-susceptible strains, the most active agents were metronidazole, tinidazole, mebendazole, furazolidone, and anisomycin. Against the resistant strain mebendazole, furazolidone, and anisomycin were the most active. Antifungal agents, beta-lactams, macrolides, aminoglycosides, and folic acid antagonists were ineffective against all strains.

Research paper thumbnail of A discrepancy between cystic echinococcosis confirmed by ultrasound and seropositivity in Turkish children

Acta Tropica

In three randomly selected villages of Manisa, Turkey, 630 primary school children were examined ... more In three randomly selected villages of Manisa, Turkey, 630 primary school children were examined for cystic echinococcosis (CE) by a portable ultrasound scanner (US) and chest microfilm. Sera samples of 483 (76.7%) children were tested for anti-E. granulosus antibodies by ELISA and indirect hemagglutination (IHA) tests. Hepatic CE were detected in two cases (0.3%) by US, while 43 (8.9%) and 49 (10.1%) cases were found to be positive for CE by ELISA and IHA, respectively. The high seropositivity levels could have been attributed to extra-abdominal or abortive Echinococcus infections, but probably most of them were false-positives. Due to the discrepancy in results, US should be selected as the primary test in field studies and serologic tests should be performed in all cases with suspected lesions. We could not find any reported community based study on CE in Turkey, in which US was applied; but our results suggest that extensive epidemiological studies are required.

Research paper thumbnail of In vitro Efficacy of Quercus infectoria Oliv. and Achillea millefolium L. Extracts Against Blastocystis spp. Isolates

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2013

ABSTRACT Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of hu... more ABSTRACT Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of humans and many animals. Despite it was firstly described almost 100 years ago; many subjects are still under debate about Blastocystis, including its life-cycle, pathogenic potential and treatment of infected individuals. Historically, local plant species have been used for therapeutic purposes by the local people of Anatolia. Here, hexane and methanol extracts of two local plants, Quercus infectoria (Fagaceae) and Achillea millefolium, which have been used against diarrhea in Anatolia, were examined for their in vitro efficacies against Blastocystis. LC50 and EC50 values of the plant extracts were determined by Brine Shrimp and Graphpad Prism 5® methods, respectively. The results showed that LC50 (500 μg/ml) and EC50 (198.8 μg/ml) concentrations of the methanol extract of A. millefolium were lowest compared to other extracts, its anti-Blastocystis activity was found to be comparable to metronidazole and it showed no cytotoxic activity. These initial results suggest that the methanol extract of A. millefolium may be a novel option for the treatment of Blastocystis infections in humans in future, if confirmed by further, larger-scale studies.

Research paper thumbnail of Incidence of Intestinal Parasites Detected in the Department of Parasitology in Celal Bayar University Hospital between 2006 and 2010

Turkish Journal of Parasitology, 2012

Selofan bant örneği alınan 5952 hastanın 253'ünde (%4.25) Enterobius vermicularis saptanmıştır. P... more Selofan bant örneği alınan 5952 hastanın 253'ünde (%4.25) Enterobius vermicularis saptanmıştır. Pozitif olguların 158'inde (%6.76) iki veya daha fazla parazit birlikte görülmüştür. Parazit saptanan kişilerin yaş ortalaması 21.9 olarak bulunmuştur. Erkeklerde parazit görülme oranı %13.6 iken kadınlarda %10.7 saptanmıştır (p<0.001). Parazit saptanan olgular yerleşim yerlerine göre değerlendirildiğinde en yüksek oranın kırsal bölgelerde yaşayanlarda (%23.8) olduğu görülmüştür (p=0.006). Sonuç: İlimizde bağırsak parazitleri arasında en sık Blastocystis spp., E. vermicularis ve G. intestinalis görülürken, son yıllarda alt yapı çalışmalarındaki ilerlemelere rağmen bağırsak parazitlerinin halen önemini koruduğu görülmüştür.

Research paper thumbnail of Evaluation of Serological Results of Patients with Suspected Toxoplasmosis Admitted to the Medical Parasitology Laboratory of Celal Bayar University Hospital between 2006-2010

Turkish Journal of Parasitology, 2012

IgG ve IgM antikorları araştırılmıştır. IgG ve IgM birlikte pozitif bulunduğu olgularda ayrıca Ig... more IgG ve IgM antikorları araştırılmıştır. IgG ve IgM birlikte pozitif bulunduğu olgularda ayrıca IgG avidite yöntemi uygulanmıştır. Bulgular: Araştırmamızda 2815 hastanın 657'sinde (%23.3) yalnız IgG pozitifliği, 4'ünde (%0.1) yalnız IgM pozitifliği saptanırken, 6 (%0.2) hastada IgG ve IgM birlikte pozitif saptanmıştır. Anti-T. gondii IgG seropozitifliği en çok çiğ et tüketen hastalarda [%21.2 (n=139)] görülmüştür. 15-49 yaş grubunda IgG seroprevalansı 0-14 yaş grubundan istatistiksel olarak yüksek bulunmuştur (p<0.05). Serolojik sonuçları beş yıllık periyoda göre değerlendirdiğimizde 2010 yılında anti-T. gondii seroprevalansının düştüğü görülmüş, bu oran istatistiksel olarak anlamlı bulunmuştur (p<0.001). Sonuç: Toxoplasma enfeksiyonunda çiğ et yeme alışkanlığının en önemli risk faktörü olduğu saptanmıştır. Beş yıllık serolojik verileri değerlendirdiğimizde son yıllarda anti-T. gondii IgG antikorlarında bir azalma saptanmış, bununla birlikte Manisa'da toxoplasmosisin halen önemli bir halk sağlığı sorunu olduğu görülmüştür.

Research paper thumbnail of Putative Bronchopulmonary Flagellated Protozoa in Immunosuppressed Patients

BioMed Research International, 2014

Flagellated protozoa that cause bronchopulmonary symptoms in humans are commonly neglected. These... more Flagellated protozoa that cause bronchopulmonary symptoms in humans are commonly neglected. These protozoal forms which were presumed to be “flagellated protozoa” have been previously identified in immunosuppressed patients in a number of studies, but have not been certainly classified so far. Since no human cases of bronchopulmonary flagellated protozoa were reported from Turkey, we aimed to investigate these putative protozoa in immunosuppressed patients who are particularly at risk of infectious diseases. Bronchoalveolar lavage fluid samples of 110 immunosuppressed adult patients who were admitted to the Department of Chest Diseases, Hafsa Sultan Hospital of Celal Bayar University, Manisa, Turkey, were examined in terms of parasites by light microscopy. Flagellated protozoal forms were detected in nine (8.2%) of 110 cases. Metronidazole (500 mgb.i.d.for 30 days) was given to all positive cases and a second bronchoscopy was performed at the end of the treatment, which revealed no ...

Research paper thumbnail of Effect of trimethoprim-sulfamethaxazole in blastocystis hominis infection

The American Journal of Gastroenterology, 1999

Blastocystis hominis (B. hominis) is a common intestinal parasite that has long been considered n... more Blastocystis hominis (B. hominis) is a common intestinal parasite that has long been considered nonpathogenic. Recently there have been many reports supporting a role for the organism as a potential pathogen. We performed a study to examine the pathogenicity of B. hominis and the effect of trimethoprim-sulfamethaxazole (TMP-SMX) on this organism. Stool samples of patients, who came to the Department of Parasitology, Faculty of Medicine, Celal Bayar University, were examined by direct wet-mount, trichrome staining, formalin-ethyl acetate concentration, and Kinyoun acid fast techniques for intestinal parasites, and bacteriological stool cultures were performed. Fifty-three symptomatic patients (38 children and 15 adults) with two consequent stool samples positive for abundant B. hominis (five or more organisms per x400 field) and negative for other parasitic and bacterial pathogens were treated with TMP-SMX for 7 days, children 6 mg/kg TMP, 30 mg/kg SMX, and adults 320 mg TMP, 1600 mg SMX, daily. On the seventh day, at the end of treatment, stool samples of all patients were examined by same methods, and clinical symptoms were again evaluated. B. hominis was eradicated in 36 of 38 (94.7%) children, and 14 of 15 (93.3%) adults. Clinical symptoms disappeared in 39 (73.6%), decreased in 10 (18.9%), and no change was observed in one (1.9%) patient, whereas symptoms persisted in all three (5.7%) patients in whom B. hominis could not be eradicated. Mean number of stools per day was significantly decreased from 4.3 to 1.2 in the 33 children (p &lt; 0.001), and decreased from 3.5 to 1.0 in the four adults (p = 0.06) with diarrhea. These results suggested that B. hominis may be pathogenic, especially when it is present in large numbers, and TMP-SMX is highly effective against this organism. Although there are some anecdotal reports, to our knowledge this is the first study examining the effect of TMP-SMX on B. hominis in humans.

Research paper thumbnail of Transmission of Dientamoeba fragilis: Evaluation of the role of Enterobius vermicularis

Parasitology International, 2008

The role of Enterobius vermicularis in the transmission of Dientamoeba fragilis has been evaluate... more The role of Enterobius vermicularis in the transmission of Dientamoeba fragilis has been evaluated in two groups of patients admitted to the Parasitology Laboratory of Celal Bayar University: one group with E. vermicularis infection (n=187, Pinworm Group), and the other with D. fragilis infection (n=126, Dientamoeba Group). The presence of the other parasite, pinworm or Dientamoeba, was investigated with the microscopic examination of cellophane tape and stool samples for three consecutive days. In the Pinworm Group, 9.6% of the patients were found to be coinfected with D. fragilis, while 25.4% of the patients in the Dientamoeba Group were found to be coinfected with pinworms. The coincidence rates of D. fragilis and E. vermicularis, higher than the prevalence of each parasite in similar populations, suggest a common relation between these two parasites, possibly in entering the human body. E. vermicularis infection was found to be significantly more common in younger children (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001), indicating that younger children may also be at higher risk for D. fragilis infection. These findings also raise the question of whether the unrelated symptoms of the pinworm infected patients such as abdominal pain and diarrhea may actually be due to overlooked Dientamoeba infections.