Ali Othmane - Academia.edu (original) (raw)
Papers by Ali Othmane
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ABSTRACT communication par affiche
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Journal of Communications Engineering and Networks, 2014
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A biosensor based on Escherichia coli Bacteria for determination of heavy metal ions with an acou... more A biosensor based on Escherichia coli Bacteria for determination of heavy metal ions with an acoustic Love wave device and Polydimethylsiloxane (PDMS) microfluidic network has been previously developed, that provides fast detection of toxic chemical compounds. Bacteria were immobilized on the transducer coated with self-assembled molecular multilayers of polyelectrolytes. In this study, we emphasize Love wave and AFM-based complementary characterization methods, which allowed an optimization of some physicochemical parameters of polyelectrolyte solutions, such as pH, ionic strength or molecular weight, in order to increase the immobilization of bacteria as well as the sensor lifetime. For instance, an increase density of bacteria was observed when using alternative pH of 9 for cationic solution.
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Talanta, Jan 15, 2011
Specific recognition between two biological partners is widely exploited in biosensors nowadays. ... more Specific recognition between two biological partners is widely exploited in biosensors nowadays. To explore this avenue, a novel biosensor for antithrombin (AT) detection was constructed. Heparin was used as the affinity ligand. A well-known acrylic monomer (butyl methacrylate) was polymerized and grafted onto the heparin polysaccharide by the use of ceric ammonium nitrate as a redox initiator in aqueous nitric acid medium. Polymers were deposited as a thin layer onto surface of stainless steel electrode (SS316L). The obtained polymers were studied by Fourier transform infrared spectroscopy (FTIR) and analyzed by differential scanning calorimetry (DSC). Moreover, the films were characterized by electrochemical impedance spectroscopy (EIS), contact-angle measurements and AFM. EIS was used to study the biosensor affinity to AT and the relationship between functionalization growth of modified electrode and the response of the sensor. The proposed approach appears to be simple, sensitiv...
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Abstract: A hybrid multisensor system combined with multivariate analysis was applied to the char... more Abstract: A hybrid multisensor system combined with multivariate analysis was applied to the characterization of different kinds of Moroccan waters. The proposed hybrid system based on an electronic nose coupled with an electronic tongue consisted of metal oxide semiconductors and potentiometric sensors respectively. Five Taguchi Gas Sensors were implemented in the electronic nose for the discrimination between mineral, natural, sparkling, river and tap waters. Afterwards, the electronic tongue, based on series of Ion-Selective-Electrodes was applied to the analysis of the same waters. Multisensor responses obtained from the waters were processed by two chemometrics: Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA). PCA results using electronic nose data depict all of the potable water samples in a separate group from the samples that were originated from river. Furthermore, PCA and LDA analysis on electronic tongue data permitted clear and rapid recognizing...
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Instrumentation Mesure Métrologie, 2013
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Sensors and Actuators B: Chemical, 2014
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Macromolecular Bioscience, 2007
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Changes in aggregability and/or deformability of red blood cells (RBC) can cause severe complicat... more Changes in aggregability and/or deformability of red blood cells (RBC) can cause severe complications in blood circulation. We use a laser light scattering technique, which can distinguish between normal and pathological RBCs by studying the angular distributions of backscattered and transmitted light of concentrated suspensions of RBCs submitted to a simple shear flow. In order to study the deformation, we induced partial rigidity in the RBC membrane, and showed that the gradients of deformation and the relaxation times of normal and partially rigidified RBC membranes can be quantified using a non-Newtonian rheological model. We observe that blood aggregation of patients with `microcirculatory' diseases, such as diabetes, differs from that of healthy individuals.
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Sensors and Actuators B: Chemical, 2014
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Sensors and Actuators B: Chemical, 2002
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Sensor Letters, 2011
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Biomolecular Engineering, 2002
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Materials Science and Engineering: C, 2006
... Quantification of the adhesion free energy between bacteria and hydrophobic and hydrophilic s... more ... Quantification of the adhesion free energy between bacteria and hydrophobic and hydrophilic substrata. ... a, Laboratoire de Physique et Chimie des Interfaces, Faculté des Sciences de Monastir,Tunisia. b, Laboratoire de Biophysique, Faculté de Médecine de Monastir, Tunisia. ...
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Bookmarks Related papers MentionsView impact
ABSTRACT communication par affiche
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Bookmarks Related papers MentionsView impact
Journal of Communications Engineering and Networks, 2014
Bookmarks Related papers MentionsView impact
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Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
A biosensor based on Escherichia coli Bacteria for determination of heavy metal ions with an acou... more A biosensor based on Escherichia coli Bacteria for determination of heavy metal ions with an acoustic Love wave device and Polydimethylsiloxane (PDMS) microfluidic network has been previously developed, that provides fast detection of toxic chemical compounds. Bacteria were immobilized on the transducer coated with self-assembled molecular multilayers of polyelectrolytes. In this study, we emphasize Love wave and AFM-based complementary characterization methods, which allowed an optimization of some physicochemical parameters of polyelectrolyte solutions, such as pH, ionic strength or molecular weight, in order to increase the immobilization of bacteria as well as the sensor lifetime. For instance, an increase density of bacteria was observed when using alternative pH of 9 for cationic solution.
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Talanta, Jan 15, 2011
Specific recognition between two biological partners is widely exploited in biosensors nowadays. ... more Specific recognition between two biological partners is widely exploited in biosensors nowadays. To explore this avenue, a novel biosensor for antithrombin (AT) detection was constructed. Heparin was used as the affinity ligand. A well-known acrylic monomer (butyl methacrylate) was polymerized and grafted onto the heparin polysaccharide by the use of ceric ammonium nitrate as a redox initiator in aqueous nitric acid medium. Polymers were deposited as a thin layer onto surface of stainless steel electrode (SS316L). The obtained polymers were studied by Fourier transform infrared spectroscopy (FTIR) and analyzed by differential scanning calorimetry (DSC). Moreover, the films were characterized by electrochemical impedance spectroscopy (EIS), contact-angle measurements and AFM. EIS was used to study the biosensor affinity to AT and the relationship between functionalization growth of modified electrode and the response of the sensor. The proposed approach appears to be simple, sensitiv...
Bookmarks Related papers MentionsView impact
Abstract: A hybrid multisensor system combined with multivariate analysis was applied to the char... more Abstract: A hybrid multisensor system combined with multivariate analysis was applied to the characterization of different kinds of Moroccan waters. The proposed hybrid system based on an electronic nose coupled with an electronic tongue consisted of metal oxide semiconductors and potentiometric sensors respectively. Five Taguchi Gas Sensors were implemented in the electronic nose for the discrimination between mineral, natural, sparkling, river and tap waters. Afterwards, the electronic tongue, based on series of Ion-Selective-Electrodes was applied to the analysis of the same waters. Multisensor responses obtained from the waters were processed by two chemometrics: Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA). PCA results using electronic nose data depict all of the potable water samples in a separate group from the samples that were originated from river. Furthermore, PCA and LDA analysis on electronic tongue data permitted clear and rapid recognizing...
Bookmarks Related papers MentionsView impact
Instrumentation Mesure Métrologie, 2013
Bookmarks Related papers MentionsView impact
Sensors and Actuators B: Chemical, 2014
Bookmarks Related papers MentionsView impact
Macromolecular Bioscience, 2007
Bookmarks Related papers MentionsView impact
Changes in aggregability and/or deformability of red blood cells (RBC) can cause severe complicat... more Changes in aggregability and/or deformability of red blood cells (RBC) can cause severe complications in blood circulation. We use a laser light scattering technique, which can distinguish between normal and pathological RBCs by studying the angular distributions of backscattered and transmitted light of concentrated suspensions of RBCs submitted to a simple shear flow. In order to study the deformation, we induced partial rigidity in the RBC membrane, and showed that the gradients of deformation and the relaxation times of normal and partially rigidified RBC membranes can be quantified using a non-Newtonian rheological model. We observe that blood aggregation of patients with `microcirculatory' diseases, such as diabetes, differs from that of healthy individuals.
Bookmarks Related papers MentionsView impact
Sensors and Actuators B: Chemical, 2014
Bookmarks Related papers MentionsView impact
Sensors and Actuators B: Chemical, 2002
Bookmarks Related papers MentionsView impact
Sensor Letters, 2011
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Biomolecular Engineering, 2002
Bookmarks Related papers MentionsView impact
Materials Science and Engineering: C, 2006
... Quantification of the adhesion free energy between bacteria and hydrophobic and hydrophilic s... more ... Quantification of the adhesion free energy between bacteria and hydrophobic and hydrophilic substrata. ... a, Laboratoire de Physique et Chimie des Interfaces, Faculté des Sciences de Monastir,Tunisia. b, Laboratoire de Biophysique, Faculté de Médecine de Monastir, Tunisia. ...
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