Shahajad Ali - Academia.edu (original) (raw)

Papers by Shahajad Ali

Research paper thumbnail of Is Non Cirrhotic Portal Fibrosis Actually Cirrhosis in Regression? A Histopathological Study of the “Hepatic Repair Complex” in Indian Patients

Journal of Clinical and Experimental Hepatology, 2016

Research paper thumbnail of Incidentally detected asymptomatic hepatitis C virus infection with significant fibrosis: Possible impacts on management

Indian journal of pathology & microbiology

Liver biopsy may be considered in patients with hepatitis C virus (HCV) infection to assess the s... more Liver biopsy may be considered in patients with hepatitis C virus (HCV) infection to assess the severity of liver injury and stage of fibrosis, thereby guiding therapeutic decisions. In addition, advanced stage also necessitates surveillance for hepatocellular carcinoma. The aim of this study was to assess whether transaminase (alanine transaminase [ALT]) levels and RNA titers correlate with the histological activity index (HAI) and fibrosis (F) stage in asymptomatic patients with incidentally detected HCV (IDHCV). Retrospective evaluation of liver biopsies was done in 113 patients with IDHCV, diagnosed during routine screening. Decision of liver biopsy was made on the basis of age, genotype, acceptable clinical, hematological, and biochemical profiles, and willingness of the patients to undergo treatment. Serum ALT levels, HCV RNA titers, and genotypes were correlated with HAI and F stage. Genotyping was done in 77 of the 113 patients, of which genotype 3 was seen in 43 and genotyp...

Research paper thumbnail of Study of histomorphological characteristics and it’s correlation with clinical, biochemical, serological and immunohistochemical parameters in incidentally detected hepatitis B patients

Annals of Pathology and Laboratory Medicine, 2017

All major liver organizations, such as the American association for the study of liver diseases (... more All major liver organizations, such as the American association for the study of liver diseases (AASLD), the European association for the study of the liver (EASL), and the Asian-Pacific association for the study of the liver (APASL), consistently recommend therapy for patients with liver damage and complications. [6, 7] However, these recommendations for treatment of chronic hepatitis B

Research paper thumbnail of Ductular Reaction and the K7-19 Progenitor Cell Sequence in Vascular Liver Disease: A New Perspective to Pathogenetic Mechanisms of Injury and Repair

Journal of Clinical and Experimental Hepatology, 2015

Research paper thumbnail of HER-2/neu over expression in gall bladder adenocarcinoma: A quest for potential therapeutic target

Indian Journal of Pathology and Microbiology, 2020

Background: Gall bladder carcinoma (GBC) is an aggressive malignancy with high mortality and aggr... more Background: Gall bladder carcinoma (GBC) is an aggressive malignancy with high mortality and aggressive course, with palliation as the only available option. Objectives: To evaluate frequency of HER-2/neu overexpression in GBC and to seek its correlation, if any with conventional clinicopathological parameters and survival. Methods: Immunohistochemistry (IHC) was performed on 200 cases of GBC, 32 cases of dysplasia, and 100 cases of chronic cholecystitis. Fluorescent in situ hybridization (FISH) was performed on 30 randomly selected cases of GBC to validate IHC. HER-2/neu overexpression (IHC 3+/FISH amplification ≥2.2) was correlated with clinicopathological parameters by Chi-square test.P < 0.05 was considered significant. Survival analysis was done by log-rank test and Kaplan-Meier analysis. Results: HER-2/neu overexpression was seen in 14% (28/200) GBC cases but was not found in dysplasia and chronic cholecystitis. Majority of these cases were ≤grade 2 and in advanced stage, however this was not statistically significant. A lower mean survival in HER-2/neu positive group as compared to HER-2/neu negative group (17.1 ± 2.3 month versus 67.6 ± 8.5 month, respectively) was observed. Concordance between IHC and FISH was seen in 18/19 cases. Conclusion: This study delineates a subset of GBC patients with HER-2/neu overexpression, in whom targeted therapy can offer a survival benefit.

Research paper thumbnail of Bioscience Horizons-2010-Bishop-85-95

Fluorescence in situ hybridization (FISH) is a powerful technique used in the detection of chromo... more Fluorescence in situ hybridization (FISH) is a powerful technique used in the detection of chromosomal abnormalities. The high sensitivity and specificity of FISH and the speed with which the assays can be performed have made FISH a pivotal cytogenetic technique that has provided significant advances in both the research and diagnosis of haematological malignancies and solid tumours. From a medical perspective, FISH can be applied to detect genetic abnormalities such as characteristic gene fusions, aneuploidy, loss of a chromosomal region or a whole chromosome or to monitor the progression of an aberration serving as a technique that can help in both the diagnosis of a genetic disease or suggesting prognostic outcomes. FISH can also be applied to such research applications as gene mapping or the identification of novel oncogenes or genetic aberrations that contribute towards various cancers. FISH is based on DNA probes annealing to specific target sequence of sample DNA. Attached to the probes are fluorescent reporter molecules which under fluorescence microscopy confirm the presence or absence of a particular genetic aberration when viewed under fluorescence microscopy. The technique has recently evolved to allow screening of the whole genome simultaneously through multicolour whole-chromosome probe techniques such as multiplex FISH or spectral karyotyping, or through an array-based method using comparative genomic hybridization. This simple, yet effective, technique has revolutionized cytogenetics and has become well established in its potential as a diagnostic and discovery tool in the fight against cancer.

Research paper thumbnail of Is Non Cirrhotic Portal Fibrosis Actually Cirrhosis in Regression? A Histopathological Study of the “Hepatic Repair Complex” in Indian Patients

Journal of Clinical and Experimental Hepatology, 2016

Research paper thumbnail of Incidentally detected asymptomatic hepatitis C virus infection with significant fibrosis: Possible impacts on management

Indian journal of pathology & microbiology

Liver biopsy may be considered in patients with hepatitis C virus (HCV) infection to assess the s... more Liver biopsy may be considered in patients with hepatitis C virus (HCV) infection to assess the severity of liver injury and stage of fibrosis, thereby guiding therapeutic decisions. In addition, advanced stage also necessitates surveillance for hepatocellular carcinoma. The aim of this study was to assess whether transaminase (alanine transaminase [ALT]) levels and RNA titers correlate with the histological activity index (HAI) and fibrosis (F) stage in asymptomatic patients with incidentally detected HCV (IDHCV). Retrospective evaluation of liver biopsies was done in 113 patients with IDHCV, diagnosed during routine screening. Decision of liver biopsy was made on the basis of age, genotype, acceptable clinical, hematological, and biochemical profiles, and willingness of the patients to undergo treatment. Serum ALT levels, HCV RNA titers, and genotypes were correlated with HAI and F stage. Genotyping was done in 77 of the 113 patients, of which genotype 3 was seen in 43 and genotyp...

Research paper thumbnail of Study of histomorphological characteristics and it’s correlation with clinical, biochemical, serological and immunohistochemical parameters in incidentally detected hepatitis B patients

Annals of Pathology and Laboratory Medicine, 2017

All major liver organizations, such as the American association for the study of liver diseases (... more All major liver organizations, such as the American association for the study of liver diseases (AASLD), the European association for the study of the liver (EASL), and the Asian-Pacific association for the study of the liver (APASL), consistently recommend therapy for patients with liver damage and complications. [6, 7] However, these recommendations for treatment of chronic hepatitis B

Research paper thumbnail of Ductular Reaction and the K7-19 Progenitor Cell Sequence in Vascular Liver Disease: A New Perspective to Pathogenetic Mechanisms of Injury and Repair

Journal of Clinical and Experimental Hepatology, 2015

Research paper thumbnail of HER-2/neu over expression in gall bladder adenocarcinoma: A quest for potential therapeutic target

Indian Journal of Pathology and Microbiology, 2020

Background: Gall bladder carcinoma (GBC) is an aggressive malignancy with high mortality and aggr... more Background: Gall bladder carcinoma (GBC) is an aggressive malignancy with high mortality and aggressive course, with palliation as the only available option. Objectives: To evaluate frequency of HER-2/neu overexpression in GBC and to seek its correlation, if any with conventional clinicopathological parameters and survival. Methods: Immunohistochemistry (IHC) was performed on 200 cases of GBC, 32 cases of dysplasia, and 100 cases of chronic cholecystitis. Fluorescent in situ hybridization (FISH) was performed on 30 randomly selected cases of GBC to validate IHC. HER-2/neu overexpression (IHC 3+/FISH amplification ≥2.2) was correlated with clinicopathological parameters by Chi-square test.P < 0.05 was considered significant. Survival analysis was done by log-rank test and Kaplan-Meier analysis. Results: HER-2/neu overexpression was seen in 14% (28/200) GBC cases but was not found in dysplasia and chronic cholecystitis. Majority of these cases were ≤grade 2 and in advanced stage, however this was not statistically significant. A lower mean survival in HER-2/neu positive group as compared to HER-2/neu negative group (17.1 ± 2.3 month versus 67.6 ± 8.5 month, respectively) was observed. Concordance between IHC and FISH was seen in 18/19 cases. Conclusion: This study delineates a subset of GBC patients with HER-2/neu overexpression, in whom targeted therapy can offer a survival benefit.

Research paper thumbnail of Bioscience Horizons-2010-Bishop-85-95

Fluorescence in situ hybridization (FISH) is a powerful technique used in the detection of chromo... more Fluorescence in situ hybridization (FISH) is a powerful technique used in the detection of chromosomal abnormalities. The high sensitivity and specificity of FISH and the speed with which the assays can be performed have made FISH a pivotal cytogenetic technique that has provided significant advances in both the research and diagnosis of haematological malignancies and solid tumours. From a medical perspective, FISH can be applied to detect genetic abnormalities such as characteristic gene fusions, aneuploidy, loss of a chromosomal region or a whole chromosome or to monitor the progression of an aberration serving as a technique that can help in both the diagnosis of a genetic disease or suggesting prognostic outcomes. FISH can also be applied to such research applications as gene mapping or the identification of novel oncogenes or genetic aberrations that contribute towards various cancers. FISH is based on DNA probes annealing to specific target sequence of sample DNA. Attached to the probes are fluorescent reporter molecules which under fluorescence microscopy confirm the presence or absence of a particular genetic aberration when viewed under fluorescence microscopy. The technique has recently evolved to allow screening of the whole genome simultaneously through multicolour whole-chromosome probe techniques such as multiplex FISH or spectral karyotyping, or through an array-based method using comparative genomic hybridization. This simple, yet effective, technique has revolutionized cytogenetics and has become well established in its potential as a diagnostic and discovery tool in the fight against cancer.