Alice Acosta - Academia.edu (original) (raw)
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Papers by Alice Acosta
Toxicon, 1994
Venom alkaline phosphatase was detected using a blotting method following electrophoresis. The en... more Venom alkaline phosphatase was detected using a blotting method following electrophoresis. The enzyme gave strong reactions in some venoms, but was absent in other venoms, some within the same species. The mol. wt of the enzyme is close to 100,000 and its pl is between 3.6 and 4.8. The enzyme was inactivated by EDTA and 2-mercaptcethanol, and lost activity by freezing and thawing. Endogenous venom alkaline phosphatase can interfere with alkaline phosphatase-based detection methods. Pre-screening for endogenous venom alkaline phosphatase is recommended prior to using alkaline phosphatasebased detection methods when studying snake venom.
Toxicon, 1994
Venom alkaline phosphatase was detected using a blotting method following electrophoresis. The en... more Venom alkaline phosphatase was detected using a blotting method following electrophoresis. The enzyme gave strong reactions in some venoms, but was absent in other venoms, some within the same species. The mol. wt of the enzyme is close to 100,000 and its pl is between 3.6 and 4.8. The enzyme was inactivated by EDTA and 2-mercaptcethanol, and lost activity by freezing and thawing. Endogenous venom alkaline phosphatase can interfere with alkaline phosphatase-based detection methods. Pre-screening for endogenous venom alkaline phosphatase is recommended prior to using alkaline phosphatasebased detection methods when studying snake venom.