Alice Tommasi Di Vignano - Academia.edu (original) (raw)

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Papers by Alice Tommasi Di Vignano

Research paper thumbnail of The ARE-binding protein Tristetraprolin (TTP) is a novel target and mediator of calcineurin tumor suppressing function in the skin

Research paper thumbnail of The FoxO3a gene is a key negative target of canonical Notch signalling in the keratinocyte UVB response

The EMBO Journal, 2008

Notch signalling has an important role in skin homeostasis, promoting keratinocyte differentiatio... more Notch signalling has an important role in skin homeostasis, promoting keratinocyte differentiation and suppressing tumorigenesis. Here we show that this pathway also has an essential anti-apoptotic function in the keratinocyte UVB response. Notch1 expression and activity are significantly induced, in a p53-dependent manner, by UVB exposure of primary keratinocytes as well as intact epidermis of both mouse and human origin. The apoptotic response to UVB is increased by deletion of the Notch1 gene or down-modulation of Notch signalling by pharmacological inhibition or genetic suppression of 'canonical' Notch/CSL/MAML1-dependent transcription. Conversely, Notch activation protects keratinocytes against apoptosis through a mechanism that is not linked to Notch-induced cell cycle withdrawal or NF-jB activation. Rather, transcription of FoxO3a, a key pro-apoptotic gene, is under direct negative control of Notch/HERP transcription in keratinocytes, and upregulation of this gene accounts for the increased susceptibility to UVB of cells with suppressed Notch signalling. Thus, the canonical Notch/HERP pathway functions as a protective anti-apoptotic mechanism in keratinocytes through negative control of FoxO3a expression.

Research paper thumbnail of Negative control of keratinocyte differentiation by Rho/CRIK signaling coupled with up-regulation of KyoT1/2 (FHL1) expression

Proceedings of the National Academy of Sciences, 2005

Rho GTPases integrate control of cell structure and adhesion with downstream signaling events. In... more Rho GTPases integrate control of cell structure and adhesion with downstream signaling events. In keratinocytes, RhoA is activated at early times of differentiation and plays an essential function in establishment of cell–cell adhesion. We report here that, surprisingly, Rho signaling suppresses downstream gene expression events associated with differentiation. Similar inhibitory effects are exerted by a specific Rho effector, CRIK (Citron kinase), which is selectively down-modulated with differentiation, thereby allowing the normal process to occur. The suppressing function of Rho/CRIK on differentiation is associated with induction of KyoT1/2, a LIM domain protein gene implicated in integrin-mediated processes and/or Notch signaling. Like activated Rho and CRIK, elevated KyoT1/2 expression suppresses differentiation. Thus, Rho signaling exerts an unexpectedly complex role in keratinocyte differentiation, which is coupled with induction of KyoT1/2, a LIM domain protein gene with a ...

Research paper thumbnail of Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation

Genes & Development, 2006

Notch signaling promotes commitment of keratinocytes to differentiation and suppresses tumorigene... more Notch signaling promotes commitment of keratinocytes to differentiation and suppresses tumorigenesis. p63, a p53 family member, has been implicated in establishment of the keratinocyte cell fate and/or maintenance of epithelial self-renewal. Here we show that p63 expression is suppressed by Notch1 activation in both mouse and human keratinocytes through a mechanism independent of cell cycle withdrawal and requiring down-modulation of selected interferon-responsive genes, including IRF7 and/or IRF3. In turn, elevated p63 expression counteracts the ability of Notch1 to restrict growth and promote differentiation. p63 functions as a selective modulator of Notch1-dependent transcription and function, with the Hes-1 gene as one of its direct negative targets. Thus, a complex cross-talk between Notch and p63 is involved in the balance between keratinocyte self-renewal and differentiation.

Research paper thumbnail of Contribution of the different modules in the utrophin carboxy-terminal region to the formation and regulation of the DAP complex

Research paper thumbnail of Integration of Notch 1 and Calcineurin/NFAT Signaling Pathways in Keratinocyte Growth and Differentiation Control

Developmental Cell, 2005

entiation (Artavanis-Tsakonas et al., 1999). The biological endpoint of activation or suppression... more entiation (Artavanis-Tsakonas et al., 1999). The biological endpoint of activation or suppression of this Lausanne University Epalinges CH-1066 pathway is critically dependent on context-specific interactions with other signaling pathways. Switzerland 2 Cutaneous Biology Research Center The Notch gene family encodes evolutionarily conserved type-1 transmembrane receptors that are acti-Massachusetts General Hospital Charlestown, Massachusetts 02129 vated by ligand binding and proteolytic cleavage, with release of the Notch intracellular domain (Artavanis

Research paper thumbnail of High Commitment of Embryonic Keratinocytes to Terminal Differentiation through a Notch1-caspase 3 Regulatory Mechanism

Research paper thumbnail of The ARE-binding protein Tristetraprolin (TTP) is a novel target and mediator of calcineurin tumor suppressing function in the skin

Research paper thumbnail of The FoxO3a gene is a key negative target of canonical Notch signalling in the keratinocyte UVB response

The EMBO Journal, 2008

Notch signalling has an important role in skin homeostasis, promoting keratinocyte differentiatio... more Notch signalling has an important role in skin homeostasis, promoting keratinocyte differentiation and suppressing tumorigenesis. Here we show that this pathway also has an essential anti-apoptotic function in the keratinocyte UVB response. Notch1 expression and activity are significantly induced, in a p53-dependent manner, by UVB exposure of primary keratinocytes as well as intact epidermis of both mouse and human origin. The apoptotic response to UVB is increased by deletion of the Notch1 gene or down-modulation of Notch signalling by pharmacological inhibition or genetic suppression of 'canonical' Notch/CSL/MAML1-dependent transcription. Conversely, Notch activation protects keratinocytes against apoptosis through a mechanism that is not linked to Notch-induced cell cycle withdrawal or NF-jB activation. Rather, transcription of FoxO3a, a key pro-apoptotic gene, is under direct negative control of Notch/HERP transcription in keratinocytes, and upregulation of this gene accounts for the increased susceptibility to UVB of cells with suppressed Notch signalling. Thus, the canonical Notch/HERP pathway functions as a protective anti-apoptotic mechanism in keratinocytes through negative control of FoxO3a expression.

Research paper thumbnail of Negative control of keratinocyte differentiation by Rho/CRIK signaling coupled with up-regulation of KyoT1/2 (FHL1) expression

Proceedings of the National Academy of Sciences, 2005

Rho GTPases integrate control of cell structure and adhesion with downstream signaling events. In... more Rho GTPases integrate control of cell structure and adhesion with downstream signaling events. In keratinocytes, RhoA is activated at early times of differentiation and plays an essential function in establishment of cell–cell adhesion. We report here that, surprisingly, Rho signaling suppresses downstream gene expression events associated with differentiation. Similar inhibitory effects are exerted by a specific Rho effector, CRIK (Citron kinase), which is selectively down-modulated with differentiation, thereby allowing the normal process to occur. The suppressing function of Rho/CRIK on differentiation is associated with induction of KyoT1/2, a LIM domain protein gene implicated in integrin-mediated processes and/or Notch signaling. Like activated Rho and CRIK, elevated KyoT1/2 expression suppresses differentiation. Thus, Rho signaling exerts an unexpectedly complex role in keratinocyte differentiation, which is coupled with induction of KyoT1/2, a LIM domain protein gene with a ...

Research paper thumbnail of Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation

Genes & Development, 2006

Notch signaling promotes commitment of keratinocytes to differentiation and suppresses tumorigene... more Notch signaling promotes commitment of keratinocytes to differentiation and suppresses tumorigenesis. p63, a p53 family member, has been implicated in establishment of the keratinocyte cell fate and/or maintenance of epithelial self-renewal. Here we show that p63 expression is suppressed by Notch1 activation in both mouse and human keratinocytes through a mechanism independent of cell cycle withdrawal and requiring down-modulation of selected interferon-responsive genes, including IRF7 and/or IRF3. In turn, elevated p63 expression counteracts the ability of Notch1 to restrict growth and promote differentiation. p63 functions as a selective modulator of Notch1-dependent transcription and function, with the Hes-1 gene as one of its direct negative targets. Thus, a complex cross-talk between Notch and p63 is involved in the balance between keratinocyte self-renewal and differentiation.

Research paper thumbnail of Contribution of the different modules in the utrophin carboxy-terminal region to the formation and regulation of the DAP complex

Research paper thumbnail of Integration of Notch 1 and Calcineurin/NFAT Signaling Pathways in Keratinocyte Growth and Differentiation Control

Developmental Cell, 2005

entiation (Artavanis-Tsakonas et al., 1999). The biological endpoint of activation or suppression... more entiation (Artavanis-Tsakonas et al., 1999). The biological endpoint of activation or suppression of this Lausanne University Epalinges CH-1066 pathway is critically dependent on context-specific interactions with other signaling pathways. Switzerland 2 Cutaneous Biology Research Center The Notch gene family encodes evolutionarily conserved type-1 transmembrane receptors that are acti-Massachusetts General Hospital Charlestown, Massachusetts 02129 vated by ligand binding and proteolytic cleavage, with release of the Notch intracellular domain (Artavanis

Research paper thumbnail of High Commitment of Embryonic Keratinocytes to Terminal Differentiation through a Notch1-caspase 3 Regulatory Mechanism