Alina Simona Sovrea - Academia.edu (original) (raw)
Papers by Alina Simona Sovrea
Molecules/Molecules online/Molecules annual, Feb 8, 2024
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
PubMed, 2017
Langerhans cell histiocytosis (LCH), previously known as "histiocytosis X", is a clinical entity ... more Langerhans cell histiocytosis (LCH), previously known as "histiocytosis X", is a clinical entity characterized by abnormal proliferation of Langerhans cells, which exert a mass effect. Orbital involvement due to LCH is rare as a unifocal disease, seldom occurring outside the pediatric population. We report a case of a 21-year-old man with solitary LCH of the orbit depicted by magnetic resonance imaging (MRI) and diagnosed by histopathological examination.
Romanian Journal of Morphology and Embryology, Apr 1, 2021
Introduction: p16 INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of ... more Introduction: p16 INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of human papillomavirus (HPV)-associated neoplasia, when ≥70% of cells show strong nuclear and cytoplasmic positivity. In this study, we aim to compare partial expression patterns that do not fulfill the above criteria and seek biological implications in laryngeal squamous cell carcinoma (LSCC). Materials and Methods: p16 INK4a IHC staining was conducted on representative sections of archived tissue from 88 LSCCs. Immunoreactivity was described based on four parameters: intracellular localization of immunostaining, intensity of immunostaining, distribution pattern and percentage of positive cells. Results: Six patterns of p16 INK4a immunoexpression were observed and defined as: strong diffuse (strong immunostaining, expression in cytoplasm and nucleus in >70% of tumor cells), weak diffuse (moderate or weak immunostaining, expression in cytoplasm in >70% of tumor cells), marginal (strong cytoplasmic immunostaining, limited to the periphery of tumor islets), strong scattered (strong immunostaining, expression in cytoplasm and nucleus in <50% of tumor cells), weak scattered (moderate or weak immunostaining, expression in cytoplasm in <50% of tumor cells), negative (no expression). The pN stage of the patients was associated with p16 INK4a immunoexpression patterns, the marginal pattern was only found in the pN0-Nx stages, while the weak diffuse pattern was more frequently observed in pN2-N3 stages. Conclusions: Partial immunostaining with architecturally distinct p16 INK4a immunoexpression patterns may prove significant in stratifying characteristic clinicopathological subgroups among LSCC. Our observations may support the hypothesis that p16 INK4a has different roles in different subcellular locations, with tumorigenic molecular pathways unrelated to HPV infection.
PubMed, 2013
Cutaneous carcinomas are malignant lesions, which most commonly occur on photo-aggressed site. Th... more Cutaneous carcinomas are malignant lesions, which most commonly occur on photo-aggressed site. The purpose of our study was to evaluate the immunohistochemical expression of three apoptotic markers (p53, Bax, and Bcl-2) in photoinduced basal and squamous cell carcinoma. The study was performed on 24 patients diagnosed with these forms of cutaneous carcinoma localized on photoexposed regions: 14 cases of basal cell carcinoma (BCC) and 10 cases of squamous cell carcinoma (SCC), classified accordingly WHO 2003. The immunohistochemical study performed on the three proteins involved in the apoptotic process revealed certain specific features in their manner of expression, which do not correlate or respect the critical determinant rule (Bcl-2/Bax>1). Basal cell carcinoma expresses higher levels of Bcl-2, with a better prognosis, a less aggressive evolution, and no metastasis. Squamous cell carcinoma, on the other hand, expresses lower levels of Bcl-2, but the clinical outcome is more aggressive, the tumor has a faster evolution and may metastasize. P53 protein respects the profile given in literature data, having a higher score in squamous cell carcinoma versus basal cell carcinoma. According to the tumor localization on photo-aggressed sites, we have considered that ultraviolet rays play an important role in initiation of carcinogenesis through still occult mechanisms that may induce these particular or rather "bizarre" expressions of apoptotic markers.
Pharmaceutics
Cancer is a leading cause of death worldwide, and the main treatment methods for this condition a... more Cancer is a leading cause of death worldwide, and the main treatment methods for this condition are surgery, chemotherapy, and radiotherapy. These treatment methods are invasive and can cause severe adverse reactions among organisms, so nanomaterials are increasingly used as structures for anticancer therapies. Dendrimers are a type of nanomaterial with unique properties, and their production can be controlled to obtain compounds with the desired characteristics. These polymeric molecules are used in cancer diagnosis and treatment through the targeted distribution of some pharmacological substances. Dendrimers have the ability to fulfill several objectives in anticancer therapy simultaneously, such as targeting tumor cells so that healthy tissue is not affected, controlling the release of anticancer agents in the tumor microenvironment, and combining anticancer strategies based on the administration of anticancer molecules to potentiate their effect through photothermal therapy or p...
Romanian Journal of Morphology and Embryology, 2021
Introduction: p16 INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of ... more Introduction: p16 INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of human papillomavirus (HPV)-associated neoplasia, when ≥70% of cells show strong nuclear and cytoplasmic positivity. In this study, we aim to compare partial expression patterns that do not fulfill the above criteria and seek biological implications in laryngeal squamous cell carcinoma (LSCC). Materials and Methods: p16 INK4a IHC staining was conducted on representative sections of archived tissue from 88 LSCCs. Immunoreactivity was described based on four parameters: intracellular localization of immunostaining, intensity of immunostaining, distribution pattern and percentage of positive cells. Results: Six patterns of p16 INK4a immunoexpression were observed and defined as: strong diffuse (strong immunostaining, expression in cytoplasm and nucleus in >70% of tumor cells), weak diffuse (moderate or weak immunostaining, expression in cytoplasm in >70% of tumor cells), marginal (strong cytoplasmic immunostaining, limited to the periphery of tumor islets), strong scattered (strong immunostaining, expression in cytoplasm and nucleus in <50% of tumor cells), weak scattered (moderate or weak immunostaining, expression in cytoplasm in <50% of tumor cells), negative (no expression). The pN stage of the patients was associated with p16 INK4a immunoexpression patterns, the marginal pattern was only found in the pN0-Nx stages, while the weak diffuse pattern was more frequently observed in pN2-N3 stages. Conclusions: Partial immunostaining with architecturally distinct p16 INK4a immunoexpression patterns may prove significant in stratifying characteristic clinicopathological subgroups among LSCC. Our observations may support the hypothesis that p16 INK4a has different roles in different subcellular locations, with tumorigenic molecular pathways unrelated to HPV infection.
Summary Argyrophilic staining of nucleolar organising regio ns (NOR) is a representation of the r... more Summary Argyrophilic staining of nucleolar organising regio ns (NOR) is a representation of the ribosomal RNA transcription, giving an image on cell proliferation. The silver-stained nucleolar organising regions (AgNOR) may be useful to reflect the degree of malignancy. The present study aimed to assess the correlation b etween the features of nucleolar organizing regions (AgNOR) in tumour cells and the degree of cell differentiation and cell proliferation. Eighteen gliomas diagnosed at the Em ergency County Hospital in Cluj-Napoca, Neurosurgery Clinic between 2007 - 2009 were studied. They were graded according to the World Health Organization grading system. The fragments were silver stained using the Crocker and Nar technique. For every tumour piece one hundred consecutive nuclei were studied, considering the morphology and the average number of AgNOR/case. The data was analysed using T-test. Data having p-value < 0.05 was considered statistically relevant. As the degree of malignancy increased, the number of visib le AgNORs was significantly elevated, their location became peripheral and their shape wa s inconstant in contrast with low-grade tumours. Differences (p<0.05) have been seen between the low and high-grade astrocytomas. Significant dissimilarity has been noticed between the grade II astrocytoma and the anaplastic astrocytoma (p<0.05) and also between the grade III astrocytoma and glioblastoma (p<0.05). The obtained numbers of AgNOR/nucleus/case in the 18 studied gliomas corresponds with other studies cited in the literature, showing a positive correlation with the histological malignancy degree. Statistical analysis allows a cl ear distinction between the two main categories: high-differentiated astrocytomas (grade s I si II) and the low-differentiated ones (grades III and IV). The data obtained by studying AgNOR can provide useful information in order to appreciate the malignancy degree and also a different approach to the prognosis and management of patients with brain cancer.
Romanian Journal of Morphology and Embryology, Sep 9, 2022
This article is a review of new advances in histology, concerning either classification or struct... more This article is a review of new advances in histology, concerning either classification or structure of different tissular elements (basement membrane, hemidesmosomes, urothelium, glandular epithelia, adipose tissue, astrocytes), and various organs' constituents (blood-brain barrier, human dental cementum, tubarial salivary glands, hepatic stellate cells, pineal gland, fibroblasts of renal interstitium, Leydig testicular cells, ovarian hilar cells), as well as novel biotechnological techniques (tissue engineering in angiogenesis), recently introduced.
Morphologie, Dec 1, 2005
Introduction Suite aux dernieres avancees scientifiques, le tissu adipeux est reconsidere du poin... more Introduction Suite aux dernieres avancees scientifiques, le tissu adipeux est reconsidere du point de vue physiologique et metabolique etant defini non seulement comme un depot de la graisse mais aussi comme un organe muni de plasticite et de dynamisme dans certaines circonstances : obesite, diabete, differentes conditions de temperature. Par consequence l’etude morphologique du tissu adipeux doit se rallier a l’ampleur de son importance physiologique. La connaissance de l’ontogenese du tissu adipeux humain est faible, surtout en ce qui concerne la differenciation et la transdifferenciation des deux types de cellules adipeuses (uniloculaires ou blanches et multiloculaires ou brunes). Materiel et methode Nous avons etudie par des techniques de microscopie photonique et electronique trois types de depots adipeux, sous-cutanes abdominaux (par definition uniloculaires), cervicaux (multiloculaires) et visceraux (retroperitoneaux et perirenaux), preleves sur des fœtus humains âges de 20 a 40 semaines gestationnelles. Resultats Qu’importe la localisation, les depots initient le developpement par des ilots de cellules condensees dans le mesenchyme, caracterisees par une riche vascularisation capillaire. Les cellules de ces ilots developpent ensuite soit des caracteristiques proches des cellules epitheliales, que nous avons denommees ilots epithelioides, soit des cellules fibroblastiques, ou ilots mesenchymateux. Nous avons remarque aussi des amas cellulaires intermediaires. Les details ultrastructurels des cellules sont bien definis en ce qui concerne la localisation sous-cutanee ou cervicale, nuances par l’aspect des mitochondries bien differentes entre les deux types de cellules. Par rapport a l’âge gestationnel, les depots visceraux restent ceux qui gardent la plus grande heterogeneite d’ilots cellulaires, confirme par l’aspect ultrastructurel de ces cellules.
Romanian Journal of Morphology and Embryology
This article focuses on the latest histological knowledge in the field regarding the peripheral l... more This article focuses on the latest histological knowledge in the field regarding the peripheral lymphoid system [mucosa-associated lymphoid tissue (MALT), bronchus-associated lymphoid tissue (BALT), gut-associated lymphoid tissue (GALT)], the thymus stroma, some of the various corpuscles of the human body (Hassall's corpuscles in thymus, arenaceous corpuscles in pineal gland, corpora amylacea in prostate and other locations) and Fañanas glial cells in the cerebellum.
Nanomaterials
Mounting evidence shows that supplementation with vitamin D and K or their analogs induces benefi... more Mounting evidence shows that supplementation with vitamin D and K or their analogs induces beneficial effects in various diseases, e.g., osteoarticular, cardiovascular, or carcinogenesis. The use of drugs delivery systems via organic and inorganic nanocarriers increases the bioavailability of vitamins and analogs, enhancing their cellular delivery and effects. The nanotechnology-based dietary supplements and drugs produced by the food and pharmaceutical industries overcome the issues associated with vitamin administration, such as stability, absorption or low bioavailability. Consequently, there is a continuous interest in optimizing the carriers’ systems in order to make them more efficient and specific for the targeted tissue. In this pioneer review, we try to circumscribe the most relevant aspects related to nanocarriers for drug delivery, compare different types of nanoparticles for vitamin D and K transportation, and critically address their benefits and disadvantages.
Romanian Journal of Morphology and Embryology, 2021
In the literature, this paper is the first to describe the use of plasma rich in growth factors (... more In the literature, this paper is the first to describe the use of plasma rich in growth factors (PRGF)–Endoret® in hemodialyzed diabetic patients, to promote the healing of after amputation wounds. The PRGF–Endoret® was primarily conceived to be used in maxillofacial surgery, oral implantology, etc., the innovation residing in the blood collection technique (quantity, moment of the week, rhythmicity), which was adapted to the specific conditions of the hemodialyzed patient. Moreover, in the initial phases, the two PRGF fractions were innovatively applied as single alternating layers on the wound surface. Only after the surface of the wound decreased, the two PRGF fractions were applied as overlapping layers. Nevertheless, the paper presents the optimal method to assess the clinical evolution of the wound. Histopathological examination of the biopsy performed during wound preparation for PRGF application brought additional, essential data for orienting the therapeutic approach. The e...
International Journal of Molecular Sciences, 2022
The tumor microenvironment is a highly dynamic accumulation of resident and infiltrating tumor ce... more The tumor microenvironment is a highly dynamic accumulation of resident and infiltrating tumor cells, responsible for growth and invasion. The authors focused on the leading-edge concepts regarding the glioblastoma microenvironment. Due to the fact that the modern trend in the research and treatment of glioblastoma is represented by multiple approaches that target not only the primary tumor but also the neighboring tissue, the study of the microenvironment in the peritumoral tissue is an appealing direction for current and future therapies.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2016
INTRODUCTION Central nervous system (CNS) germ cell tumors are very rare, accounting for 0.3-3% o... more INTRODUCTION Central nervous system (CNS) germ cell tumors are very rare, accounting for 0.3-3% of primary intracranial neoplasms; of these, the teratomas are even more uncommon. The immature variant of teratomas, defined by the presence of incompletely differentiated components resembling fetal tissues is considered as having a low, almost borderline malignancy state. CASE PRESENTATION A 35-year-old male presented with a left fronto-basal tumor. At surgery, a grey white tumor, mostly solid, was excised. The histopathological examination revealed an infiltrating teratoma. The histological spectrum varied from epithelial and mesenchymal mature to immature tissues. These structures were intimately mixed with significant areas of primitive neuroepithelial tubules and÷or primitive neuroectodermal tissues. The diagnosis was that of an immature intracranial teratoma, with high histological grade WHO (World Health Organization) (Norris grade III). After surgical resection, a rapid infraten...
page no: 6 www.ommegaonline.org Vol: 5 Issue: 1 Abstract Root resorption is the loss of hard dent... more page no: 6 www.ommegaonline.org Vol: 5 Issue: 1 Abstract Root resorption is the loss of hard dental structures under the action of clastic cells, which can also be accompanied by bone tissue loss. Root resorption can be external or internal. In both situations, root resorption requires the presence of blood supply allowing migration of clastic cells. In internal root resorption, pulp vitality is required at least in the apical third of the root canal.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2019
Nowadays, adipose tissue appears to be the most valuable source in regenerative cell therapy, due... more Nowadays, adipose tissue appears to be the most valuable source in regenerative cell therapy, due to the following characteristics: high accessibility, high expression in a large number of individuals, high self-renewal and ability to differentiate, and hematopoietic support to the implant area. Its therapeutic potential has been experimentally observed in a broad spectrum of diseases with high population impact: diabetes, myocardial infarction, Parkinson disease, bone fractures, facial reconstruction or loss of subcutaneous tissue due to congenital abnormalities (e.g., hemifacial microsomy), trauma, burns, and tumors. Over 130 clinical trials using adipose-derived stem cells (ASCs), majority phase I or phase II, have been registered with the National Institutes of Health (NIH), and in the short term no adverse reactions or significant risks were identified. Parallel with regulatory frameworks that control their safety and assess their efficacy, phase III trials are being developed....
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2017
The present study refers to a quantitative, morphometric analysis of exfoliative cytology smears ... more The present study refers to a quantitative, morphometric analysis of exfoliative cytology smears collected from diabetes mellitus (DM) patients, in order to distinguish subtle changes in cellular and nuclear parameters. The study was carried out on 30 adult subjects: a control group of 10 healthy subjects and a study group of 20 diabetic subjects (type 1 and type 2 DM). Another factor that was taken into consideration was the abundance of the microbial flora. The oral smears were stained using Hematoxylin and Eosin and several parameters were measured (nuclear diameter, perimeter and area, cell large diameter and area), and calculated: nuclear/cytoplasmic ratio and nuclear roundness factor. We found out that the cells collected from DM patients had higher values of the nuclear parameters (the nuclei were larger) and lower cell dimensions. The nuclear to cytoplasmic ratio was increased in these patients, but the nuclear roundness factor was closer to one in the study group. Also, an ...
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2016
In this study, we performed microscopic qualitative analyses of the oral epithelium cytological s... more In this study, we performed microscopic qualitative analyses of the oral epithelium cytological smears in potential early phase of diabetes and in type 1 and 2 diabetic patients versus a healthy control group. The cytological assessment of the oral changes was realized on superficial and profound smears, from jugal and ventral tongue mucosa and it was based on the comparison between three staining methods [Papanicolaou, APT (polychrome tannin blue)-Dragan and Hematoxylin-Eosin (HE)]. Cytological changes of oral cells population were correlated with the type, duration and complications of diabetes. Oral flora was also evaluated. Irrespective the staining used, we found a clear dividing line between the control group and the real diabetic patients. In all diabetes cases (independently of the type of smear, harvest site, clinical form of disorder and present complications), cells presented alterations both at the level of cytoplasm and nucleus. Dyschromasia, cytolysis, different degree...
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2017
INTRODUCTION Keratoacanthoma (KA) is a relatively common, benign, rapidly growing and self-limiti... more INTRODUCTION Keratoacanthoma (KA) is a relatively common, benign, rapidly growing and self-limiting squamous proliferation, which appears most frequently on the sun-exposed skin. The nature of KA and its relationship to squamous cell carcinoma (SCC) still represent one of the major debates in dermatopathology, as it is the truthfulness of such a diagnosis outside the skin. However, the tumor is now known to originate from the pilosebaceous units of the skin or from ectopic sebaceous glands of squamous mucous membranes, and to differentiate onto follicular isthmus÷infundibulum-like epithelium. CASE PRESENTATION A 71-year-old man presented with a sore and red right eye, which on slit-lamp biomicroscopical examination revealed a dome-shaped lesion at the temporal inferior conjunctival limbus. After a thorough histopathological examination, a diagnosis of KA has been made, both after the initial tumor excision and after the relatively rapid recurrence. After the second intervention, no ...
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2018
Giant cell granulomas in the oral cavity are reactive hyperplastic lesions that arise either peri... more Giant cell granulomas in the oral cavity are reactive hyperplastic lesions that arise either peripherally in the mucoperiosteum or centrally in the bone. The peripheral giant cell granuloma (PGCG) is a benign lesion induced by local chronic irritation. It may develop at any age, and tends to be more frequent in females. Central giant cell granuloma (CGCG) is a reactive lesion of unknown etiology. It commonly occurs in children and young adults. It is also predominant in females and frequently located in the anterior part of the mandible. Histologically, PGCG and CGCG have similar features. The lesions are non-encapsulated proliferations of oval and spindle-shaped mononuclear cells (MCs) and multiple multinucleated giant cells (MGCs) in a vascular supporting stromal tissue, associated with foci of hemorrhage. Despite the similar microscopic features, PGCG and CGCG have different clinical behavior. PGCG is usually reduced in size and asymptomatic. It grows locally, as an exophytic les...
Molecules/Molecules online/Molecules annual, Feb 8, 2024
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
PubMed, 2017
Langerhans cell histiocytosis (LCH), previously known as "histiocytosis X", is a clinical entity ... more Langerhans cell histiocytosis (LCH), previously known as "histiocytosis X", is a clinical entity characterized by abnormal proliferation of Langerhans cells, which exert a mass effect. Orbital involvement due to LCH is rare as a unifocal disease, seldom occurring outside the pediatric population. We report a case of a 21-year-old man with solitary LCH of the orbit depicted by magnetic resonance imaging (MRI) and diagnosed by histopathological examination.
Romanian Journal of Morphology and Embryology, Apr 1, 2021
Introduction: p16 INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of ... more Introduction: p16 INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of human papillomavirus (HPV)-associated neoplasia, when ≥70% of cells show strong nuclear and cytoplasmic positivity. In this study, we aim to compare partial expression patterns that do not fulfill the above criteria and seek biological implications in laryngeal squamous cell carcinoma (LSCC). Materials and Methods: p16 INK4a IHC staining was conducted on representative sections of archived tissue from 88 LSCCs. Immunoreactivity was described based on four parameters: intracellular localization of immunostaining, intensity of immunostaining, distribution pattern and percentage of positive cells. Results: Six patterns of p16 INK4a immunoexpression were observed and defined as: strong diffuse (strong immunostaining, expression in cytoplasm and nucleus in >70% of tumor cells), weak diffuse (moderate or weak immunostaining, expression in cytoplasm in >70% of tumor cells), marginal (strong cytoplasmic immunostaining, limited to the periphery of tumor islets), strong scattered (strong immunostaining, expression in cytoplasm and nucleus in <50% of tumor cells), weak scattered (moderate or weak immunostaining, expression in cytoplasm in <50% of tumor cells), negative (no expression). The pN stage of the patients was associated with p16 INK4a immunoexpression patterns, the marginal pattern was only found in the pN0-Nx stages, while the weak diffuse pattern was more frequently observed in pN2-N3 stages. Conclusions: Partial immunostaining with architecturally distinct p16 INK4a immunoexpression patterns may prove significant in stratifying characteristic clinicopathological subgroups among LSCC. Our observations may support the hypothesis that p16 INK4a has different roles in different subcellular locations, with tumorigenic molecular pathways unrelated to HPV infection.
PubMed, 2013
Cutaneous carcinomas are malignant lesions, which most commonly occur on photo-aggressed site. Th... more Cutaneous carcinomas are malignant lesions, which most commonly occur on photo-aggressed site. The purpose of our study was to evaluate the immunohistochemical expression of three apoptotic markers (p53, Bax, and Bcl-2) in photoinduced basal and squamous cell carcinoma. The study was performed on 24 patients diagnosed with these forms of cutaneous carcinoma localized on photoexposed regions: 14 cases of basal cell carcinoma (BCC) and 10 cases of squamous cell carcinoma (SCC), classified accordingly WHO 2003. The immunohistochemical study performed on the three proteins involved in the apoptotic process revealed certain specific features in their manner of expression, which do not correlate or respect the critical determinant rule (Bcl-2/Bax>1). Basal cell carcinoma expresses higher levels of Bcl-2, with a better prognosis, a less aggressive evolution, and no metastasis. Squamous cell carcinoma, on the other hand, expresses lower levels of Bcl-2, but the clinical outcome is more aggressive, the tumor has a faster evolution and may metastasize. P53 protein respects the profile given in literature data, having a higher score in squamous cell carcinoma versus basal cell carcinoma. According to the tumor localization on photo-aggressed sites, we have considered that ultraviolet rays play an important role in initiation of carcinogenesis through still occult mechanisms that may induce these particular or rather "bizarre" expressions of apoptotic markers.
Pharmaceutics
Cancer is a leading cause of death worldwide, and the main treatment methods for this condition a... more Cancer is a leading cause of death worldwide, and the main treatment methods for this condition are surgery, chemotherapy, and radiotherapy. These treatment methods are invasive and can cause severe adverse reactions among organisms, so nanomaterials are increasingly used as structures for anticancer therapies. Dendrimers are a type of nanomaterial with unique properties, and their production can be controlled to obtain compounds with the desired characteristics. These polymeric molecules are used in cancer diagnosis and treatment through the targeted distribution of some pharmacological substances. Dendrimers have the ability to fulfill several objectives in anticancer therapy simultaneously, such as targeting tumor cells so that healthy tissue is not affected, controlling the release of anticancer agents in the tumor microenvironment, and combining anticancer strategies based on the administration of anticancer molecules to potentiate their effect through photothermal therapy or p...
Romanian Journal of Morphology and Embryology, 2021
Introduction: p16 INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of ... more Introduction: p16 INK4a immunohistochemistry (IHC) is widely used to facilitate the diagnosis of human papillomavirus (HPV)-associated neoplasia, when ≥70% of cells show strong nuclear and cytoplasmic positivity. In this study, we aim to compare partial expression patterns that do not fulfill the above criteria and seek biological implications in laryngeal squamous cell carcinoma (LSCC). Materials and Methods: p16 INK4a IHC staining was conducted on representative sections of archived tissue from 88 LSCCs. Immunoreactivity was described based on four parameters: intracellular localization of immunostaining, intensity of immunostaining, distribution pattern and percentage of positive cells. Results: Six patterns of p16 INK4a immunoexpression were observed and defined as: strong diffuse (strong immunostaining, expression in cytoplasm and nucleus in >70% of tumor cells), weak diffuse (moderate or weak immunostaining, expression in cytoplasm in >70% of tumor cells), marginal (strong cytoplasmic immunostaining, limited to the periphery of tumor islets), strong scattered (strong immunostaining, expression in cytoplasm and nucleus in <50% of tumor cells), weak scattered (moderate or weak immunostaining, expression in cytoplasm in <50% of tumor cells), negative (no expression). The pN stage of the patients was associated with p16 INK4a immunoexpression patterns, the marginal pattern was only found in the pN0-Nx stages, while the weak diffuse pattern was more frequently observed in pN2-N3 stages. Conclusions: Partial immunostaining with architecturally distinct p16 INK4a immunoexpression patterns may prove significant in stratifying characteristic clinicopathological subgroups among LSCC. Our observations may support the hypothesis that p16 INK4a has different roles in different subcellular locations, with tumorigenic molecular pathways unrelated to HPV infection.
Summary Argyrophilic staining of nucleolar organising regio ns (NOR) is a representation of the r... more Summary Argyrophilic staining of nucleolar organising regio ns (NOR) is a representation of the ribosomal RNA transcription, giving an image on cell proliferation. The silver-stained nucleolar organising regions (AgNOR) may be useful to reflect the degree of malignancy. The present study aimed to assess the correlation b etween the features of nucleolar organizing regions (AgNOR) in tumour cells and the degree of cell differentiation and cell proliferation. Eighteen gliomas diagnosed at the Em ergency County Hospital in Cluj-Napoca, Neurosurgery Clinic between 2007 - 2009 were studied. They were graded according to the World Health Organization grading system. The fragments were silver stained using the Crocker and Nar technique. For every tumour piece one hundred consecutive nuclei were studied, considering the morphology and the average number of AgNOR/case. The data was analysed using T-test. Data having p-value < 0.05 was considered statistically relevant. As the degree of malignancy increased, the number of visib le AgNORs was significantly elevated, their location became peripheral and their shape wa s inconstant in contrast with low-grade tumours. Differences (p<0.05) have been seen between the low and high-grade astrocytomas. Significant dissimilarity has been noticed between the grade II astrocytoma and the anaplastic astrocytoma (p<0.05) and also between the grade III astrocytoma and glioblastoma (p<0.05). The obtained numbers of AgNOR/nucleus/case in the 18 studied gliomas corresponds with other studies cited in the literature, showing a positive correlation with the histological malignancy degree. Statistical analysis allows a cl ear distinction between the two main categories: high-differentiated astrocytomas (grade s I si II) and the low-differentiated ones (grades III and IV). The data obtained by studying AgNOR can provide useful information in order to appreciate the malignancy degree and also a different approach to the prognosis and management of patients with brain cancer.
Romanian Journal of Morphology and Embryology, Sep 9, 2022
This article is a review of new advances in histology, concerning either classification or struct... more This article is a review of new advances in histology, concerning either classification or structure of different tissular elements (basement membrane, hemidesmosomes, urothelium, glandular epithelia, adipose tissue, astrocytes), and various organs' constituents (blood-brain barrier, human dental cementum, tubarial salivary glands, hepatic stellate cells, pineal gland, fibroblasts of renal interstitium, Leydig testicular cells, ovarian hilar cells), as well as novel biotechnological techniques (tissue engineering in angiogenesis), recently introduced.
Morphologie, Dec 1, 2005
Introduction Suite aux dernieres avancees scientifiques, le tissu adipeux est reconsidere du poin... more Introduction Suite aux dernieres avancees scientifiques, le tissu adipeux est reconsidere du point de vue physiologique et metabolique etant defini non seulement comme un depot de la graisse mais aussi comme un organe muni de plasticite et de dynamisme dans certaines circonstances : obesite, diabete, differentes conditions de temperature. Par consequence l’etude morphologique du tissu adipeux doit se rallier a l’ampleur de son importance physiologique. La connaissance de l’ontogenese du tissu adipeux humain est faible, surtout en ce qui concerne la differenciation et la transdifferenciation des deux types de cellules adipeuses (uniloculaires ou blanches et multiloculaires ou brunes). Materiel et methode Nous avons etudie par des techniques de microscopie photonique et electronique trois types de depots adipeux, sous-cutanes abdominaux (par definition uniloculaires), cervicaux (multiloculaires) et visceraux (retroperitoneaux et perirenaux), preleves sur des fœtus humains âges de 20 a 40 semaines gestationnelles. Resultats Qu’importe la localisation, les depots initient le developpement par des ilots de cellules condensees dans le mesenchyme, caracterisees par une riche vascularisation capillaire. Les cellules de ces ilots developpent ensuite soit des caracteristiques proches des cellules epitheliales, que nous avons denommees ilots epithelioides, soit des cellules fibroblastiques, ou ilots mesenchymateux. Nous avons remarque aussi des amas cellulaires intermediaires. Les details ultrastructurels des cellules sont bien definis en ce qui concerne la localisation sous-cutanee ou cervicale, nuances par l’aspect des mitochondries bien differentes entre les deux types de cellules. Par rapport a l’âge gestationnel, les depots visceraux restent ceux qui gardent la plus grande heterogeneite d’ilots cellulaires, confirme par l’aspect ultrastructurel de ces cellules.
Romanian Journal of Morphology and Embryology
This article focuses on the latest histological knowledge in the field regarding the peripheral l... more This article focuses on the latest histological knowledge in the field regarding the peripheral lymphoid system [mucosa-associated lymphoid tissue (MALT), bronchus-associated lymphoid tissue (BALT), gut-associated lymphoid tissue (GALT)], the thymus stroma, some of the various corpuscles of the human body (Hassall's corpuscles in thymus, arenaceous corpuscles in pineal gland, corpora amylacea in prostate and other locations) and Fañanas glial cells in the cerebellum.
Nanomaterials
Mounting evidence shows that supplementation with vitamin D and K or their analogs induces benefi... more Mounting evidence shows that supplementation with vitamin D and K or their analogs induces beneficial effects in various diseases, e.g., osteoarticular, cardiovascular, or carcinogenesis. The use of drugs delivery systems via organic and inorganic nanocarriers increases the bioavailability of vitamins and analogs, enhancing their cellular delivery and effects. The nanotechnology-based dietary supplements and drugs produced by the food and pharmaceutical industries overcome the issues associated with vitamin administration, such as stability, absorption or low bioavailability. Consequently, there is a continuous interest in optimizing the carriers’ systems in order to make them more efficient and specific for the targeted tissue. In this pioneer review, we try to circumscribe the most relevant aspects related to nanocarriers for drug delivery, compare different types of nanoparticles for vitamin D and K transportation, and critically address their benefits and disadvantages.
Romanian Journal of Morphology and Embryology, 2021
In the literature, this paper is the first to describe the use of plasma rich in growth factors (... more In the literature, this paper is the first to describe the use of plasma rich in growth factors (PRGF)–Endoret® in hemodialyzed diabetic patients, to promote the healing of after amputation wounds. The PRGF–Endoret® was primarily conceived to be used in maxillofacial surgery, oral implantology, etc., the innovation residing in the blood collection technique (quantity, moment of the week, rhythmicity), which was adapted to the specific conditions of the hemodialyzed patient. Moreover, in the initial phases, the two PRGF fractions were innovatively applied as single alternating layers on the wound surface. Only after the surface of the wound decreased, the two PRGF fractions were applied as overlapping layers. Nevertheless, the paper presents the optimal method to assess the clinical evolution of the wound. Histopathological examination of the biopsy performed during wound preparation for PRGF application brought additional, essential data for orienting the therapeutic approach. The e...
International Journal of Molecular Sciences, 2022
The tumor microenvironment is a highly dynamic accumulation of resident and infiltrating tumor ce... more The tumor microenvironment is a highly dynamic accumulation of resident and infiltrating tumor cells, responsible for growth and invasion. The authors focused on the leading-edge concepts regarding the glioblastoma microenvironment. Due to the fact that the modern trend in the research and treatment of glioblastoma is represented by multiple approaches that target not only the primary tumor but also the neighboring tissue, the study of the microenvironment in the peritumoral tissue is an appealing direction for current and future therapies.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2016
INTRODUCTION Central nervous system (CNS) germ cell tumors are very rare, accounting for 0.3-3% o... more INTRODUCTION Central nervous system (CNS) germ cell tumors are very rare, accounting for 0.3-3% of primary intracranial neoplasms; of these, the teratomas are even more uncommon. The immature variant of teratomas, defined by the presence of incompletely differentiated components resembling fetal tissues is considered as having a low, almost borderline malignancy state. CASE PRESENTATION A 35-year-old male presented with a left fronto-basal tumor. At surgery, a grey white tumor, mostly solid, was excised. The histopathological examination revealed an infiltrating teratoma. The histological spectrum varied from epithelial and mesenchymal mature to immature tissues. These structures were intimately mixed with significant areas of primitive neuroepithelial tubules and÷or primitive neuroectodermal tissues. The diagnosis was that of an immature intracranial teratoma, with high histological grade WHO (World Health Organization) (Norris grade III). After surgical resection, a rapid infraten...
page no: 6 www.ommegaonline.org Vol: 5 Issue: 1 Abstract Root resorption is the loss of hard dent... more page no: 6 www.ommegaonline.org Vol: 5 Issue: 1 Abstract Root resorption is the loss of hard dental structures under the action of clastic cells, which can also be accompanied by bone tissue loss. Root resorption can be external or internal. In both situations, root resorption requires the presence of blood supply allowing migration of clastic cells. In internal root resorption, pulp vitality is required at least in the apical third of the root canal.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2019
Nowadays, adipose tissue appears to be the most valuable source in regenerative cell therapy, due... more Nowadays, adipose tissue appears to be the most valuable source in regenerative cell therapy, due to the following characteristics: high accessibility, high expression in a large number of individuals, high self-renewal and ability to differentiate, and hematopoietic support to the implant area. Its therapeutic potential has been experimentally observed in a broad spectrum of diseases with high population impact: diabetes, myocardial infarction, Parkinson disease, bone fractures, facial reconstruction or loss of subcutaneous tissue due to congenital abnormalities (e.g., hemifacial microsomy), trauma, burns, and tumors. Over 130 clinical trials using adipose-derived stem cells (ASCs), majority phase I or phase II, have been registered with the National Institutes of Health (NIH), and in the short term no adverse reactions or significant risks were identified. Parallel with regulatory frameworks that control their safety and assess their efficacy, phase III trials are being developed....
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2017
The present study refers to a quantitative, morphometric analysis of exfoliative cytology smears ... more The present study refers to a quantitative, morphometric analysis of exfoliative cytology smears collected from diabetes mellitus (DM) patients, in order to distinguish subtle changes in cellular and nuclear parameters. The study was carried out on 30 adult subjects: a control group of 10 healthy subjects and a study group of 20 diabetic subjects (type 1 and type 2 DM). Another factor that was taken into consideration was the abundance of the microbial flora. The oral smears were stained using Hematoxylin and Eosin and several parameters were measured (nuclear diameter, perimeter and area, cell large diameter and area), and calculated: nuclear/cytoplasmic ratio and nuclear roundness factor. We found out that the cells collected from DM patients had higher values of the nuclear parameters (the nuclei were larger) and lower cell dimensions. The nuclear to cytoplasmic ratio was increased in these patients, but the nuclear roundness factor was closer to one in the study group. Also, an ...
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2016
In this study, we performed microscopic qualitative analyses of the oral epithelium cytological s... more In this study, we performed microscopic qualitative analyses of the oral epithelium cytological smears in potential early phase of diabetes and in type 1 and 2 diabetic patients versus a healthy control group. The cytological assessment of the oral changes was realized on superficial and profound smears, from jugal and ventral tongue mucosa and it was based on the comparison between three staining methods [Papanicolaou, APT (polychrome tannin blue)-Dragan and Hematoxylin-Eosin (HE)]. Cytological changes of oral cells population were correlated with the type, duration and complications of diabetes. Oral flora was also evaluated. Irrespective the staining used, we found a clear dividing line between the control group and the real diabetic patients. In all diabetes cases (independently of the type of smear, harvest site, clinical form of disorder and present complications), cells presented alterations both at the level of cytoplasm and nucleus. Dyschromasia, cytolysis, different degree...
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2017
INTRODUCTION Keratoacanthoma (KA) is a relatively common, benign, rapidly growing and self-limiti... more INTRODUCTION Keratoacanthoma (KA) is a relatively common, benign, rapidly growing and self-limiting squamous proliferation, which appears most frequently on the sun-exposed skin. The nature of KA and its relationship to squamous cell carcinoma (SCC) still represent one of the major debates in dermatopathology, as it is the truthfulness of such a diagnosis outside the skin. However, the tumor is now known to originate from the pilosebaceous units of the skin or from ectopic sebaceous glands of squamous mucous membranes, and to differentiate onto follicular isthmus÷infundibulum-like epithelium. CASE PRESENTATION A 71-year-old man presented with a sore and red right eye, which on slit-lamp biomicroscopical examination revealed a dome-shaped lesion at the temporal inferior conjunctival limbus. After a thorough histopathological examination, a diagnosis of KA has been made, both after the initial tumor excision and after the relatively rapid recurrence. After the second intervention, no ...
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2018
Giant cell granulomas in the oral cavity are reactive hyperplastic lesions that arise either peri... more Giant cell granulomas in the oral cavity are reactive hyperplastic lesions that arise either peripherally in the mucoperiosteum or centrally in the bone. The peripheral giant cell granuloma (PGCG) is a benign lesion induced by local chronic irritation. It may develop at any age, and tends to be more frequent in females. Central giant cell granuloma (CGCG) is a reactive lesion of unknown etiology. It commonly occurs in children and young adults. It is also predominant in females and frequently located in the anterior part of the mandible. Histologically, PGCG and CGCG have similar features. The lesions are non-encapsulated proliferations of oval and spindle-shaped mononuclear cells (MCs) and multiple multinucleated giant cells (MGCs) in a vascular supporting stromal tissue, associated with foci of hemorrhage. Despite the similar microscopic features, PGCG and CGCG have different clinical behavior. PGCG is usually reduced in size and asymptomatic. It grows locally, as an exophytic les...