Alka Bali - Academia.edu (original) (raw)
Papers by Alka Bali
Rapid communications in mass spectrometry/RCM. Rapid communications in mass spectrometry, Feb 13, 2024
Journal of Applied Spectroscopy, Jan 7, 2024
Journal of Applied Spectroscopy, Aug 31, 2023
In vitro drug release studies (Franz diffusion cell) with the transdermal patches. Table S2 Ex vi... more In vitro drug release studies (Franz diffusion cell) with the transdermal patches. Table S2 Ex vivo drug release studies (Franz diffusion cell) with the transdermal patches. Figure S1 Percent in vitro release at various time intervals for formulations A1–A4, B1–B4, C1–C4. Figure S2 Percent ex vivo release at various time intervals for formulations A1–A4, B1–B4, C1–C4. (DOCX 281 kb)
Advances in bioinformatics and biomedical engineering book series, Oct 1, 2023
Journal of Applied Spectroscopy, Jun 6, 2022
A selective and sensitive spectrofluorimetric method was developed and validated for the determin... more A selective and sensitive spectrofluorimetric method was developed and validated for the determination of amoxapine in human plasma and urine. The developed method is based on labeling with 5-dimethylaminonaphthalene-1-sulfonyl chloride (dansyl chloride) and monitoring at 397 nm (excitation)/514 nm (emission). The method was validated for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, recovery and robustness. The calibration curves were linear over a concentration range of 250-2500 and 50-1250 ng/mL for plasma and urine, respectively. The LOD values were calculated to be 13.31 and 13.17 ng/mL for plasma and urine, respectively. The proposed method was applied to study of amoxapine in human plasma and urine.
British journal of pharmaceutical research, Jan 10, 2015
International Journal of Mass Spectrometry, Aug 1, 2023
Journal of Analytical Science and Technology, Nov 28, 2016
Background: Duloxetine hydrochloride is an antidepressant drug also approved for diabetic neuropa... more Background: Duloxetine hydrochloride is an antidepressant drug also approved for diabetic neuropathy, anxiety disorders, and fibromyalgia requiring repeated administration on chronic basis. The objective of this study was to develop a transdermal drug delivery system for duloxetine hydrochloride as a once daily dosage form. Methods: Transdermal patches were prepared by solvent evaporation method employing controlled release grades of HPMC in presence or absence of plasticizer PEG-400. FTIR and Differential scanning calorimetry ruled out drug polymer interactions. Standard procedures were used to analyze the prepared films for various physicochemical parameters, drug release (Franz diffusion cell) and skin irritation test. Results: The formulations were uniform in their physical characteristics with low water vapor absorption, moisture loss and water vapor transmission implying excellent quality and uniformity in patch characteristics. The patches were devoid of hypersensitivity reactions on rat skin. The in-vitro and ex-vivo drug release studies for all the formulations showed that the first dose of the drug was released in 2.0-3.0 h and nearly complete release (94%) was achieved in 24 h. Conclusions: Transdermal patches were successfully prepared for duloxetine hydrochloride and their evaluation suggested excellent quality and uniformity in patch characteristics. This can have potential applications in therapeutic arena offering advantages in terms of reduced dosing frequency, improved patient compliance and bioavailability.
ChemInform, Sep 9, 2010
Inhibitors of the coagulation factor XIIa (FXIIa) are attractive to detail the roles of this prot... more Inhibitors of the coagulation factor XIIa (FXIIa) are attractive to detail the roles of this protease in hemostasis and thrombosis, to suppress artifact due to contact pathway activation in blood coagulation assays, and they are promising as antithrombotic therapy. The 3-carboxamide coumarins have been previously described as small-molecular-weight FXIIa inhibitors. In this study, we report a structureactivity relationship (SAR) study around this scaffold with the aim to discover new selective FXIIa inhibitors with an improved physico-chemical profile. To better understand these SAR, docking experiments were undertaken. For this purpose, we built an original hybrid model of FXIIa. This model has the advantage to gather the best features from the recently published crystal structure of FXIIa in its zymogen form and a more classical homology model. Results with the hybrid model are encouraging as they help understanding the activity and selectivity of our best compounds.
Der Pharmacia Lettre, 2015
Duloxetine (DLX), is a selective serotonin-norepinephrine reuptake inhibitor (SNRI) recommended f... more Duloxetine (DLX), is a selective serotonin-norepinephrine reuptake inhibitor (SNRI) recommended for maintenance treatment of major depressive disorder, neuropathic pain especially diabetic polyneuropathy (first-line treatment), generalized anxiety disorder, stress urinary incontinence and fibromyalgia. The present investigation describes the validation of rapid, sensitive, cost effective and reproducible stability indicating spectrofluorometric methods based on the native fluorescence of duloxetine HCl in acidic medium for the estimation of duloxetine HCl in bulk and in formulations. The fluorescence intensity of duloxetine hydrochloride was measured at 336 nm after excitation at 290 nm. The methods were validated with respect to linearity, accuracy, precision and robustness. Linearity was observed in the concentration range of 0.3-30 µg/ml with an excellent correlation coefficients (r 2) ranging from 0.9940-0.9996. The limits of assay detection values were found to range from 0.56-0.89 µg/ml and quantitation limits ranged from 1.69-2.42 µg/ml for the proposed methods. The proposed method was applicable to the determination of the drug in capsules and the percentage recovery was found to range from 99.53 ± 99.66%. The proposed methods were developed as stability indicating procedures by carrying out the analysis for duloxetine hydrochloride on stressed samples prepared under various forced degradation conditions.
Journal of Applied Spectroscopy, 2023
Journal of Applied Spectroscopy, May 1, 2016
Rapid, sensitive, cost effective and reproducible stability-indicating derivative spectrophotomet... more Rapid, sensitive, cost effective and reproducible stability-indicating derivative spectrophotometric methods have been developed for the estimation of dronedarone HCl employing peak–zero (P–0) and peak–peak (P–P) techniques, and their stability-indicating potential assessed in forced degraded solutions of the drug. The methods were validated with respect to linearity, accuracy, precision and robustness. Excellent linearity was observed in concentrations 2–40 μg/ml (r2 = 0.9986). LOD and LOQ values for the proposed methods ranged from 0.42–0.46 μg/ml and 1.21–1.27 μg/ml, respectively, and excellent recovery of the drug was obtained in the tablet samples (99.70 ± 0.84%).
Journal of Applied Spectroscopy
Journal of Applied Spectroscopy, Nov 3, 2021
Rapid Communications in Mass Spectrometry
Journal of Applied Spectroscopy
Rapid communications in mass spectrometry/RCM. Rapid communications in mass spectrometry, Feb 13, 2024
Journal of Applied Spectroscopy, Jan 7, 2024
Journal of Applied Spectroscopy, Aug 31, 2023
In vitro drug release studies (Franz diffusion cell) with the transdermal patches. Table S2 Ex vi... more In vitro drug release studies (Franz diffusion cell) with the transdermal patches. Table S2 Ex vivo drug release studies (Franz diffusion cell) with the transdermal patches. Figure S1 Percent in vitro release at various time intervals for formulations A1–A4, B1–B4, C1–C4. Figure S2 Percent ex vivo release at various time intervals for formulations A1–A4, B1–B4, C1–C4. (DOCX 281 kb)
Advances in bioinformatics and biomedical engineering book series, Oct 1, 2023
Journal of Applied Spectroscopy, Jun 6, 2022
A selective and sensitive spectrofluorimetric method was developed and validated for the determin... more A selective and sensitive spectrofluorimetric method was developed and validated for the determination of amoxapine in human plasma and urine. The developed method is based on labeling with 5-dimethylaminonaphthalene-1-sulfonyl chloride (dansyl chloride) and monitoring at 397 nm (excitation)/514 nm (emission). The method was validated for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, recovery and robustness. The calibration curves were linear over a concentration range of 250-2500 and 50-1250 ng/mL for plasma and urine, respectively. The LOD values were calculated to be 13.31 and 13.17 ng/mL for plasma and urine, respectively. The proposed method was applied to study of amoxapine in human plasma and urine.
British journal of pharmaceutical research, Jan 10, 2015
International Journal of Mass Spectrometry, Aug 1, 2023
Journal of Analytical Science and Technology, Nov 28, 2016
Background: Duloxetine hydrochloride is an antidepressant drug also approved for diabetic neuropa... more Background: Duloxetine hydrochloride is an antidepressant drug also approved for diabetic neuropathy, anxiety disorders, and fibromyalgia requiring repeated administration on chronic basis. The objective of this study was to develop a transdermal drug delivery system for duloxetine hydrochloride as a once daily dosage form. Methods: Transdermal patches were prepared by solvent evaporation method employing controlled release grades of HPMC in presence or absence of plasticizer PEG-400. FTIR and Differential scanning calorimetry ruled out drug polymer interactions. Standard procedures were used to analyze the prepared films for various physicochemical parameters, drug release (Franz diffusion cell) and skin irritation test. Results: The formulations were uniform in their physical characteristics with low water vapor absorption, moisture loss and water vapor transmission implying excellent quality and uniformity in patch characteristics. The patches were devoid of hypersensitivity reactions on rat skin. The in-vitro and ex-vivo drug release studies for all the formulations showed that the first dose of the drug was released in 2.0-3.0 h and nearly complete release (94%) was achieved in 24 h. Conclusions: Transdermal patches were successfully prepared for duloxetine hydrochloride and their evaluation suggested excellent quality and uniformity in patch characteristics. This can have potential applications in therapeutic arena offering advantages in terms of reduced dosing frequency, improved patient compliance and bioavailability.
ChemInform, Sep 9, 2010
Inhibitors of the coagulation factor XIIa (FXIIa) are attractive to detail the roles of this prot... more Inhibitors of the coagulation factor XIIa (FXIIa) are attractive to detail the roles of this protease in hemostasis and thrombosis, to suppress artifact due to contact pathway activation in blood coagulation assays, and they are promising as antithrombotic therapy. The 3-carboxamide coumarins have been previously described as small-molecular-weight FXIIa inhibitors. In this study, we report a structureactivity relationship (SAR) study around this scaffold with the aim to discover new selective FXIIa inhibitors with an improved physico-chemical profile. To better understand these SAR, docking experiments were undertaken. For this purpose, we built an original hybrid model of FXIIa. This model has the advantage to gather the best features from the recently published crystal structure of FXIIa in its zymogen form and a more classical homology model. Results with the hybrid model are encouraging as they help understanding the activity and selectivity of our best compounds.
Der Pharmacia Lettre, 2015
Duloxetine (DLX), is a selective serotonin-norepinephrine reuptake inhibitor (SNRI) recommended f... more Duloxetine (DLX), is a selective serotonin-norepinephrine reuptake inhibitor (SNRI) recommended for maintenance treatment of major depressive disorder, neuropathic pain especially diabetic polyneuropathy (first-line treatment), generalized anxiety disorder, stress urinary incontinence and fibromyalgia. The present investigation describes the validation of rapid, sensitive, cost effective and reproducible stability indicating spectrofluorometric methods based on the native fluorescence of duloxetine HCl in acidic medium for the estimation of duloxetine HCl in bulk and in formulations. The fluorescence intensity of duloxetine hydrochloride was measured at 336 nm after excitation at 290 nm. The methods were validated with respect to linearity, accuracy, precision and robustness. Linearity was observed in the concentration range of 0.3-30 µg/ml with an excellent correlation coefficients (r 2) ranging from 0.9940-0.9996. The limits of assay detection values were found to range from 0.56-0.89 µg/ml and quantitation limits ranged from 1.69-2.42 µg/ml for the proposed methods. The proposed method was applicable to the determination of the drug in capsules and the percentage recovery was found to range from 99.53 ± 99.66%. The proposed methods were developed as stability indicating procedures by carrying out the analysis for duloxetine hydrochloride on stressed samples prepared under various forced degradation conditions.
Journal of Applied Spectroscopy, 2023
Journal of Applied Spectroscopy, May 1, 2016
Rapid, sensitive, cost effective and reproducible stability-indicating derivative spectrophotomet... more Rapid, sensitive, cost effective and reproducible stability-indicating derivative spectrophotometric methods have been developed for the estimation of dronedarone HCl employing peak–zero (P–0) and peak–peak (P–P) techniques, and their stability-indicating potential assessed in forced degraded solutions of the drug. The methods were validated with respect to linearity, accuracy, precision and robustness. Excellent linearity was observed in concentrations 2–40 μg/ml (r2 = 0.9986). LOD and LOQ values for the proposed methods ranged from 0.42–0.46 μg/ml and 1.21–1.27 μg/ml, respectively, and excellent recovery of the drug was obtained in the tablet samples (99.70 ± 0.84%).
Journal of Applied Spectroscopy
Journal of Applied Spectroscopy, Nov 3, 2021
Rapid Communications in Mass Spectrometry
Journal of Applied Spectroscopy