Fátima Amado - Academia.edu (original) (raw)

Papers by Fátima Amado

Research paper thumbnail of Hematopoietic progenitor cells collection in pediatric patients with brain tumor

Journal of Clinical Apheresis, Apr 7, 2015

Research paper thumbnail of Stem cells out of the bag: characterization of <i>ex vivo</i> expanded mesenchymal stromal cells for possible clinical use

Future Science OA, Mar 1, 2020

Research paper thumbnail of Peripheral blood stem cells collection by apheresis in very low‐weight children with malignant diseases—A single center experience

Journal of Clinical Apheresis, Oct 9, 2022

BackgroundHematopoietic stem cell transplantation is used in the treatment of children with malig... more BackgroundHematopoietic stem cell transplantation is used in the treatment of children with malignant and non‐malignant diseases. However, apheresis of peripheral blood stem cells (PBSC) represents a challenge in children below 10 kg.MethodsA retrospective trial was conducted in the Cellular Therapy Department of Portuguese Oncology Institute of Porto. Inclusion criteria: children with body weight inferior to 10 kg who underwent autologous PBSC apheresis until 2021. Demographic and clinical data were collected and our institutional protocol was described. COBE Spectra apheresis system (TerumoBCT, Lakewood, Colorado) until 2012 and then Spectra Optia (TerumoBCT) were used.ResultsSixteen leukocytaphereses were performed in 13 patients—nine females (69%). Mean age and weight were 13.31 months (±5.26) and 8.31 kg (±1.17), respectively. The initial CD34+ cells/μL in peripheral blood was 70.8 (±61.9). A central venous catheter (CVC) was exclusively used in all but one patient, in whom a peripheral vein was also required. In 10 procedures, both heparin and anticoagulant citrate dextrose solution—formula A (ACD‐A) were used; in the remaining ones, only ACD‐A was employed. The median duration of each procedure was 168 minutes (±45) and 2.96 blood volumes (±0.31) were processed. Median CD34+ cells yield per leukocytapheresis was 6.52 × 106/kg (±9.87 × 106). CD34+ cell extraction rates were not significantly different between the two apheresis systems. Platelet and magnesium levels were significantly lower after collection (P &lt; .001 and P = .009, respectively).ConclusionsAlthough recommendations are lacking, we have successfully and safely performed leukocytaphereses in children with body weight below 10 kg. The authors believe that a permanent and dynamic comprehensive evaluation of each child is paramount for attaining good results.

Research paper thumbnail of 54 Satisfaction of unrelated donor after haematopoietic stem cell collection

Transfusion and Apheresis Science, 2010

Research paper thumbnail of Peripheral blood stem cells collection by apheresis in very low‐weight children with malignant diseases—A single center experience

Journal of Clinical Apheresis

Research paper thumbnail of As vantagens da implementação do sistema de gestão da qualidade em estabelecimentos hospitalares

A implementação de um Sistema de Gestão da Qualidade em estabelecimentos hospitalares e a avaliaç... more A implementação de um Sistema de Gestão da Qualidade em estabelecimentos hospitalares e a avaliação das suas vantagens revela-se de sublime pertinência, enquanto geradora de um contributo para a melhoria contínua da qualidade. Este estudo teve como objetivo caraterizar o universo representacional dos profissionais e dos utentes/doentes do Instituto Português de Oncologia do Porto Francisco Gentil, EPE e objetivar de que forma a implementação de um Sistema de Gestão da Qualidade contribui para uma perceção positiva da qualidade dos serviços prestados aos doentes, bem como das vantagens desta no desempenho dos profissionais e na satisfação dos utentes.info:eu-repo/semantics/updatedVersio

Research paper thumbnail of Some Techniques in Studying Rates of Deposition and Formation of Inorganic Films on Metal Surfaces

Transactions of the IMF, 1959

Research paper thumbnail of Transplante de Progenitores Hematopoiéticos: Consumo Transfusional

ARQUIVOS DE MEDICINA, 21(3/4):91-6 O objectivo deste estudo foi avaliar, nos doentes submetidos a... more ARQUIVOS DE MEDICINA, 21(3/4):91-6 O objectivo deste estudo foi avaliar, nos doentes submetidos a transplante de progenitores hematopoiéticos (TPH) alogénico, o efeito do regime de condicionamento, da fonte de progenitores, das compatibilidades ABO e HLA e do parentesco do par dador/receptor no consumo transfusional no período pós-transplante. Avaliamos retrospectivamente os doentes submetidos ao primeiro alotransplante no período compreendido entre Janeiro 2003 e Setembro 2005. Consultaram-se as bases de dados do Departamento de Imuno-Hemoterapia. A análise estatística foi realizada com recurso ao programa SPSS. No período considerado realizaram-se 97 alotransplantes, dos quais 94 foram primeiros transplantes e elegíveis para o estudo (43,6% sexo feminino e 56,4% sexo masculino, mediana de idades de 38,5 anos, limites 6 meses-66 anos). Foram submetidos a um regime de condicionamento mieloablativo 50 doentes (53,2%) e a condicionamento de intensidade reduzida (CIR) 44 doentes (46,8%...

Research paper thumbnail of Transplante de Progenitores Hematopoiéticos: Consumo Transfusional

Arquivos de Medicina

Avaliamos retrospectivamente os doentes submetidos ao primeiro alotransplante no período compreen... more Avaliamos retrospectivamente os doentes submetidos ao primeiro alotransplante no período compreendido entre Janeiro 2003 e Setembro 2005. Consultaram-se as bases de dados do Departamento de Imuno-Hemoterapia. A análise estatística foi realizada com recurso ao ...

Research paper thumbnail of Hematopoietic progenitor cells collection in pediatric patients with brain tumor

Journal of clinical apheresis, Jan 7, 2015

To analyze the efficacy and safety of hematopoietic progenitor cells (HPC) collections by leukaph... more To analyze the efficacy and safety of hematopoietic progenitor cells (HPC) collections by leukapheresis in pediatric patients with brain tumors. Between 2003 and 2014, we collected HPC from 19 children (12 boys/7 girls), median age at the diagnosis of 5 years old (<1-15 years old) and weight of 16.8 Kg (6.7-42). Diagnoses were Medulloblastoma (n = 10), Primitive Neuroectodermal Tumor (n = 5), Atypical Teratoid Rhabdoid Tumor (n = 3) and Secreting Germ Cell Tumor (n = 1). All patients performed leukapheresis by a central venous catheter, at the fifth day of mobilization with G-CSF (median dose 11.7 µg/Kg/day), 9 of them with COBE® Spectra and 10 with Spectra Optia®. The anticoagulant used was ACD-A, ratio of 14:1 or ACD-A plus heparin, ratio 25:1. The tubing set was primed with a compatible, irradiated, leukodepleted and hematocrit adjusted packed red cells for all children <30 kg (n = 17). A median of 1.5 (1-3) leukapheresis per patient was performed with an average of 3 (1.5-...

Research paper thumbnail of Stem cells out of the bag: characterization of ex vivo expanded mesenchymal stromal cells for possible clinical use

Future Science OA

Aim: Mesenchymal stromal cells (MSC) are a promising tool for cellular therapy and regenerative m... more Aim: Mesenchymal stromal cells (MSC) are a promising tool for cellular therapy and regenerative medicine. One major difficulty in establishing a MSC expansion protocol is the large volume of bone marrow (BM) required. We studied whether cells trapped within a collection bag and filter system could be considered as a source of MSC. Results: From the 20 BM collection bag and filter systems, we recovered an average of 1.68 × 108 mononuclear cells, which is the equivalent to 60 ml of filtered BM. Mononuclear cells were expanded ex vivo to 17 × 106 MSC, with purity shown by a CD44+, CD105+, CD90+ and CD73+ immunophenotype, a reduction of 20% proliferating cells in a mixed lymphocyte reaction and also the ability of adipocyte differentiation. Conclusion: Long-term MSC cultures were established from the usually discarded BM collection bag and filter, maintaining an appropriate phenotype and function, being suitable for both investigation and clinical settings.

Research paper thumbnail of Hematopoietic progenitor cells collection in pediatric patients with brain tumor

Journal of Clinical Apheresis, Apr 7, 2015

Research paper thumbnail of Stem cells out of the bag: characterization of <i>ex vivo</i> expanded mesenchymal stromal cells for possible clinical use

Future Science OA, Mar 1, 2020

Research paper thumbnail of Peripheral blood stem cells collection by apheresis in very low‐weight children with malignant diseases—A single center experience

Journal of Clinical Apheresis, Oct 9, 2022

BackgroundHematopoietic stem cell transplantation is used in the treatment of children with malig... more BackgroundHematopoietic stem cell transplantation is used in the treatment of children with malignant and non‐malignant diseases. However, apheresis of peripheral blood stem cells (PBSC) represents a challenge in children below 10 kg.MethodsA retrospective trial was conducted in the Cellular Therapy Department of Portuguese Oncology Institute of Porto. Inclusion criteria: children with body weight inferior to 10 kg who underwent autologous PBSC apheresis until 2021. Demographic and clinical data were collected and our institutional protocol was described. COBE Spectra apheresis system (TerumoBCT, Lakewood, Colorado) until 2012 and then Spectra Optia (TerumoBCT) were used.ResultsSixteen leukocytaphereses were performed in 13 patients—nine females (69%). Mean age and weight were 13.31 months (±5.26) and 8.31 kg (±1.17), respectively. The initial CD34+ cells/μL in peripheral blood was 70.8 (±61.9). A central venous catheter (CVC) was exclusively used in all but one patient, in whom a peripheral vein was also required. In 10 procedures, both heparin and anticoagulant citrate dextrose solution—formula A (ACD‐A) were used; in the remaining ones, only ACD‐A was employed. The median duration of each procedure was 168 minutes (±45) and 2.96 blood volumes (±0.31) were processed. Median CD34+ cells yield per leukocytapheresis was 6.52 × 106/kg (±9.87 × 106). CD34+ cell extraction rates were not significantly different between the two apheresis systems. Platelet and magnesium levels were significantly lower after collection (P &lt; .001 and P = .009, respectively).ConclusionsAlthough recommendations are lacking, we have successfully and safely performed leukocytaphereses in children with body weight below 10 kg. The authors believe that a permanent and dynamic comprehensive evaluation of each child is paramount for attaining good results.

Research paper thumbnail of 54 Satisfaction of unrelated donor after haematopoietic stem cell collection

Transfusion and Apheresis Science, 2010

Research paper thumbnail of Peripheral blood stem cells collection by apheresis in very low‐weight children with malignant diseases—A single center experience

Journal of Clinical Apheresis

Research paper thumbnail of As vantagens da implementação do sistema de gestão da qualidade em estabelecimentos hospitalares

A implementação de um Sistema de Gestão da Qualidade em estabelecimentos hospitalares e a avaliaç... more A implementação de um Sistema de Gestão da Qualidade em estabelecimentos hospitalares e a avaliação das suas vantagens revela-se de sublime pertinência, enquanto geradora de um contributo para a melhoria contínua da qualidade. Este estudo teve como objetivo caraterizar o universo representacional dos profissionais e dos utentes/doentes do Instituto Português de Oncologia do Porto Francisco Gentil, EPE e objetivar de que forma a implementação de um Sistema de Gestão da Qualidade contribui para uma perceção positiva da qualidade dos serviços prestados aos doentes, bem como das vantagens desta no desempenho dos profissionais e na satisfação dos utentes.info:eu-repo/semantics/updatedVersio

Research paper thumbnail of Some Techniques in Studying Rates of Deposition and Formation of Inorganic Films on Metal Surfaces

Transactions of the IMF, 1959

Research paper thumbnail of Transplante de Progenitores Hematopoiéticos: Consumo Transfusional

ARQUIVOS DE MEDICINA, 21(3/4):91-6 O objectivo deste estudo foi avaliar, nos doentes submetidos a... more ARQUIVOS DE MEDICINA, 21(3/4):91-6 O objectivo deste estudo foi avaliar, nos doentes submetidos a transplante de progenitores hematopoiéticos (TPH) alogénico, o efeito do regime de condicionamento, da fonte de progenitores, das compatibilidades ABO e HLA e do parentesco do par dador/receptor no consumo transfusional no período pós-transplante. Avaliamos retrospectivamente os doentes submetidos ao primeiro alotransplante no período compreendido entre Janeiro 2003 e Setembro 2005. Consultaram-se as bases de dados do Departamento de Imuno-Hemoterapia. A análise estatística foi realizada com recurso ao programa SPSS. No período considerado realizaram-se 97 alotransplantes, dos quais 94 foram primeiros transplantes e elegíveis para o estudo (43,6% sexo feminino e 56,4% sexo masculino, mediana de idades de 38,5 anos, limites 6 meses-66 anos). Foram submetidos a um regime de condicionamento mieloablativo 50 doentes (53,2%) e a condicionamento de intensidade reduzida (CIR) 44 doentes (46,8%...

Research paper thumbnail of Transplante de Progenitores Hematopoiéticos: Consumo Transfusional

Arquivos de Medicina

Avaliamos retrospectivamente os doentes submetidos ao primeiro alotransplante no período compreen... more Avaliamos retrospectivamente os doentes submetidos ao primeiro alotransplante no período compreendido entre Janeiro 2003 e Setembro 2005. Consultaram-se as bases de dados do Departamento de Imuno-Hemoterapia. A análise estatística foi realizada com recurso ao ...

Research paper thumbnail of Hematopoietic progenitor cells collection in pediatric patients with brain tumor

Journal of clinical apheresis, Jan 7, 2015

To analyze the efficacy and safety of hematopoietic progenitor cells (HPC) collections by leukaph... more To analyze the efficacy and safety of hematopoietic progenitor cells (HPC) collections by leukapheresis in pediatric patients with brain tumors. Between 2003 and 2014, we collected HPC from 19 children (12 boys/7 girls), median age at the diagnosis of 5 years old (<1-15 years old) and weight of 16.8 Kg (6.7-42). Diagnoses were Medulloblastoma (n = 10), Primitive Neuroectodermal Tumor (n = 5), Atypical Teratoid Rhabdoid Tumor (n = 3) and Secreting Germ Cell Tumor (n = 1). All patients performed leukapheresis by a central venous catheter, at the fifth day of mobilization with G-CSF (median dose 11.7 µg/Kg/day), 9 of them with COBE® Spectra and 10 with Spectra Optia®. The anticoagulant used was ACD-A, ratio of 14:1 or ACD-A plus heparin, ratio 25:1. The tubing set was primed with a compatible, irradiated, leukodepleted and hematocrit adjusted packed red cells for all children <30 kg (n = 17). A median of 1.5 (1-3) leukapheresis per patient was performed with an average of 3 (1.5-...

Research paper thumbnail of Stem cells out of the bag: characterization of ex vivo expanded mesenchymal stromal cells for possible clinical use

Future Science OA

Aim: Mesenchymal stromal cells (MSC) are a promising tool for cellular therapy and regenerative m... more Aim: Mesenchymal stromal cells (MSC) are a promising tool for cellular therapy and regenerative medicine. One major difficulty in establishing a MSC expansion protocol is the large volume of bone marrow (BM) required. We studied whether cells trapped within a collection bag and filter system could be considered as a source of MSC. Results: From the 20 BM collection bag and filter systems, we recovered an average of 1.68 × 108 mononuclear cells, which is the equivalent to 60 ml of filtered BM. Mononuclear cells were expanded ex vivo to 17 × 106 MSC, with purity shown by a CD44+, CD105+, CD90+ and CD73+ immunophenotype, a reduction of 20% proliferating cells in a mixed lymphocyte reaction and also the ability of adipocyte differentiation. Conclusion: Long-term MSC cultures were established from the usually discarded BM collection bag and filter, maintaining an appropriate phenotype and function, being suitable for both investigation and clinical settings.