Amal Yacoub - Academia.edu (original) (raw)

Papers by Amal Yacoub

Research paper thumbnail of Biochemical and Histopathological Changes in the Liver of Oreochromis niloticus Experimentaly Infected with Escherichia coli

Research Advances in Microbiology and Biotechnology Vol. 1, Dec 28, 2022

Research paper thumbnail of Impact of the water quality of El-Rahawy Drain on some g enetic and histopathological aspects of Oreochromis niloticus

Egyptian Journal of Aquatic Biology and Fisheries, 2020

The present study aims to evaluate the water quality of two locations in Rosetta Branch of River ... more The present study aims to evaluate the water quality of two locations in Rosetta Branch of River Nile and its histopathological and genetic adverse effects on Nile tilapia (Oreochromis niloticus). Water and fish samples were collected from up and downstream of El-Rahawy Drain discharge point (location I and II, respectively) during the summer and winter seasons. The water quality, liver histopathology and DNA alteration of fish was examined. ISSR-PCR technique was used to assess the genetic variation of fish samples from these locations. The water quality parameters (BOD, COD,……)were increased at location II compared with location I in addition to depletion in dissolved oxygen. Moreover, the fish samples collected from location II showed severe histological and molecular alterations than those collected from location I. It is recommended that the government must increase the awareness of people around the River Nile, and improve the environmental management to reduce the risks of polluted water and consuming the fish exposed to this water on human health.

Research paper thumbnail of Meat Quality and Hematological Indices of Oreochromis niloticus Fish Experimentally Exposed to Escherichia coli Toxins: A Recent Study

Recent Research Advances in Biology Vol. 11, 2021

Research paper thumbnail of Accumulation of some heavy metals and biochemical alterations in muscles of Oreochromis niloticus from the River Nile in Upper Egypt

Research paper thumbnail of Biochemical, Histopathological, and Genetic Impacts of River Nile Pollutants on the Nile tilapia (Oreochromis niloticus)

Egyptian Journal of Aquatic Biology and Fisheries, 2021

Water pollution is one of the most principal environmental and public health problems in the Rive... more Water pollution is one of the most principal environmental and public health problems in the River Nile. In the present study, aims were directed to evaluate the biochemical, histopathological, and genetic effects of El- Rahawy Drain pollutants on the Nile tilapia (Oreochromis niloticus). Hence, during the summer of 2018 and winter 2019, fish samples were collected from two locations (upstream and downstream) of El-Rahawy drain in the River Nile at the Rosetta Branch. Biochemical studies (Total Protein, Albumin, Globulin, A/G, total lipids, Cholesterol, AST, and ALT) were carried out for the specimens and important results were revealed. Compared with the fish caught from the upstream, a deterioration was detected in the health status of the downstream- fish; displaying more extreme histopathological and molecular variations. Using SSR (simple sequence repeats) to detect the molecular effects of water pollutants on O. niloticus, molecular markers indicated genetic differences in fi...

Research paper thumbnail of Heavy Metals and Macronutrients Concentration in Oreochromis Njloticus and Tilapia Zilliifish Species Inhabiting Some Egyptian Lakes and El-Salam Canal

Egyptian Journal of Aquatic Biology and Fisheries, 2005

T he present study was initiated to assess the levels of Fe, Mn, Zn. Cu. Ni. Co. Pb and Cd togeth... more T he present study was initiated to assess the levels of Fe, Mn, Zn. Cu. Ni. Co. Pb and Cd together with Na, K, Ca and Mg in liver and muscle of Tilapia zillii from three different aquatic ecosystems namely. Lake Manzaiah (Brackish water). Lake Qarun (Saline water) and El-Salam Canal (fresh water) and Oreochromis niloticus from Lake Manzaiah and El-Salam Canal throughout four seasons (1999-2H00). The present results revealed that liver has higher tendency to accumulate Fe and Cu more than the other studied heavy metals for O. niloticus and T. zillii in different studied sites. Zn followed Fe in the accumulation abundance in muscle tissues of the two studied species and Cd was the lowest one. The fluctuation of metal concentrations in muscle tissues for O. niloticus and T. zillii exhibited the following descending order Fe>Zn>Mn=Cu-Ni=Co=Pb>Cd. while the abundance order of the same metals in liver tissues was: Fe>Cu>Zn>Mn>Ni>Co= Pb>Cd.

Research paper thumbnail of Heavy Lmetals Accumulation and Macronutrients in the Livers of Some Fish Species of Bardawil Lagoon and Their Histological Changes

Egyptian Journal of Aquatic Biology and Fisheries, 2003

F ish samples of Solea vulgaris. Mugil cephalus, Liza ramada and Spams aurata were collected duri... more F ish samples of Solea vulgaris. Mugil cephalus, Liza ramada and Spams aurata were collected during November 2001 from El Telol location in Bardawil lagoon, north eastern of Egyptian Mediterranean coast. The study was conducted to assess the effect of the heavy metals (Fe. Mn. Zm Cm Pb and Cd) and the macro nutrients (Na, K, Ca, and Mg) on livers tissues of the four fish species. The results showed that the concentrations of Fe, Mn, Zn, Cu, Pb, and Cd in the livers of the four fish species ranged between 48.

Research paper thumbnail of Pathological changes in liver structure and function of Oreochromis niloticus experimentally exposed to Escherichia coli

International Journal of Biotechnology and Bioengineering, 2017

Authman et al. (2013) noticed increase in glutamate-oxaloacetate transaminase (GOT) and glutamate... more Authman et al. (2013) noticed increase in glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities and different biochemical parameters of clarias gariepinus living in El-Rahawy drain. Also, histological study cleared that liver of C. gariepinus living in El-Rahawy drain suffered from several pathological alterations. All these findings were discussed and referred to the input of agricultural, industrial and sewage water into El-Rahawy drain.

Research paper thumbnail of Accumulation of heavy metals in tilapia fish species and related histopathological changes in muscles, gills and liver of Oreochromis niloticus occurring in the area of Qahr El-Bahr, Lake Al-Manzalah, Egypt

Oceanological and Hydrobiological Studies, 2021

Accumulation of metals (Fe, Zn, Cu, Mn, Pb, Cd and Ni) in the muscles and gills of Oreochromis ni... more Accumulation of metals (Fe, Zn, Cu, Mn, Pb, Cd and Ni) in the muscles and gills of Oreochromis niloticus, Oreochromis aureus and Tilapia zillii was assessed based on seven locations in the Qahr El-Bahr area in Lake Al-Manzalah. The average accumulation of metals was in the following order: Tilapia zillii > Oreochromis aureus > Oreochromis niloticus. To determine the risk of fish consumption, the estimated daily intake, carcinogenic, non-carcinogenic and relative risk indices were calculated. The results indicate that the intake of individual metals through the consumption of fish is almost safe for human health, whereas the intake of combined metals poses a high potential health risk to consumers. Assessment of carcinogenic risk of Cd and Ni from the consumption of tilapia species indicates that consumers are at risk of cancer. The contribution of Pb and Cd to the overall relative risk index ranged from 34% to 41%. Of the three species studied, Oreochromis niloticus is relativ...

Research paper thumbnail of Injuries in Kidney Tissue of the Mugil Fish Liza Ramada as a Biomarker for Environmental Pollution in the Lake Manzalah

Egyptian Journal of Aquatic Biology and Fisheries, 2003

T his study deals with the effect of aquatic environmental pollution of Lake Manzalah on Lizarama... more T his study deals with the effect of aquatic environmental pollution of Lake Manzalah on Lizaramada. The fish and water samples were collected seasonally for a year from Autumn 2000 to Summer 2001 from the selected stations near Boughaz El-Gamil. Other water samples were collected from 12 different stations in other locations. The study included heavy metal analysis of the water lake and histopathological changes in the kidneys of the same fish. The investigation revealed that the high values of heavy metals (Fe, Mn, Cu, Zn, Pb and Cd) encountered during the different seasons in lake Manzalah induced toxic effects on Lizaramada kidneys which may decrease the productivity of this Mugil species. So, it is recommended to try to protect this fish from the destruction by drainage water loaded with agricultural and industrial pollutants as well as sewage. 0 Shadia M. Kadry et ah considered as a sink for disposing industrial and human wastes. A total amount of about 7500 million cubic meters of untreated industrial domestic and drainage water as well as agrochemicals (fertilizers and biocides) is discharged annually into the lake through seven main drains (Ibrahim etal, 1997). Hadous, Ramsis and Bahr El-Bakar drains contribute about 75% of the total annual drainage water into the southern sector of the lake. Previous studies on the hydrography and chemical properties of Lake Manzalah have been reported by El Wakeel and Wahby (1970) and Wahby et al (1972). They concluded that the drainage water changed the nutrient level of the lake in many respects especially the nitrate phosphate ratio. The residue levels of petroleum hydrocarbons, chlorinated insecticides, polychlorinated biphenyls and organochlorine pesticides in lake Manzalah were studied by Abou-Arab et al (1995) and Badawye/tf/. (1995). Abdel-Satar (2001) studied the effect of drains on the southern of lake Manzalah. The study revealed depletion of dissolved oxygen and high concentrations of nutrients, chemical oxygen demand, biochemical oxygen demand and heavy metals. As an indicator of exposure to contaminants, histology represents a useful tool to assess the degree of pollution (Bernet et al, 1999). The kidney of fish is a suitable organ for histological examination in order to determine the effect of pollution (Bucher and Hofer, 1993). Many authors reported histopathological abnormalities in kidney of different fish species that were exposed to several pollutants (agricultural, industrial and sewage) (Daskalow etal t 2000 and Lliopoulou & Kotsani, 2001). The present study is initiated as a framework to seek an overview of the impact of water pollution in lake Manzalah on the histopathological changes in Liza ramada kidney. In order to proceed to asound assessment of this effect, it was first necessary to monitor the levels of heavy metals pollution in the lake throughout the year by four seasons. MATERIAL AND METHODS A-Collection And Preparation of Water Samples: Water samples were collected seasonally from Autumn 2000 to Sximmer 2001. Twelve stations were selected to cover the area of the lake and divided into four sectors. The location of sampling stations are presented in Fig(l). The samples were collected from

Research paper thumbnail of Biochemical and Histopathological Changes in the Liver of Oreochromis niloticus Experimentaly Infected with Escherichia coli

Research Advances in Microbiology and Biotechnology Vol. 1, Dec 28, 2022

Research paper thumbnail of Impact of the water quality of El-Rahawy Drain on some g enetic and histopathological aspects of Oreochromis niloticus

Egyptian Journal of Aquatic Biology and Fisheries, 2020

The present study aims to evaluate the water quality of two locations in Rosetta Branch of River ... more The present study aims to evaluate the water quality of two locations in Rosetta Branch of River Nile and its histopathological and genetic adverse effects on Nile tilapia (Oreochromis niloticus). Water and fish samples were collected from up and downstream of El-Rahawy Drain discharge point (location I and II, respectively) during the summer and winter seasons. The water quality, liver histopathology and DNA alteration of fish was examined. ISSR-PCR technique was used to assess the genetic variation of fish samples from these locations. The water quality parameters (BOD, COD,……)were increased at location II compared with location I in addition to depletion in dissolved oxygen. Moreover, the fish samples collected from location II showed severe histological and molecular alterations than those collected from location I. It is recommended that the government must increase the awareness of people around the River Nile, and improve the environmental management to reduce the risks of polluted water and consuming the fish exposed to this water on human health.

Research paper thumbnail of Meat Quality and Hematological Indices of Oreochromis niloticus Fish Experimentally Exposed to Escherichia coli Toxins: A Recent Study

Recent Research Advances in Biology Vol. 11, 2021

Research paper thumbnail of Accumulation of some heavy metals and biochemical alterations in muscles of Oreochromis niloticus from the River Nile in Upper Egypt

Research paper thumbnail of Biochemical, Histopathological, and Genetic Impacts of River Nile Pollutants on the Nile tilapia (Oreochromis niloticus)

Egyptian Journal of Aquatic Biology and Fisheries, 2021

Water pollution is one of the most principal environmental and public health problems in the Rive... more Water pollution is one of the most principal environmental and public health problems in the River Nile. In the present study, aims were directed to evaluate the biochemical, histopathological, and genetic effects of El- Rahawy Drain pollutants on the Nile tilapia (Oreochromis niloticus). Hence, during the summer of 2018 and winter 2019, fish samples were collected from two locations (upstream and downstream) of El-Rahawy drain in the River Nile at the Rosetta Branch. Biochemical studies (Total Protein, Albumin, Globulin, A/G, total lipids, Cholesterol, AST, and ALT) were carried out for the specimens and important results were revealed. Compared with the fish caught from the upstream, a deterioration was detected in the health status of the downstream- fish; displaying more extreme histopathological and molecular variations. Using SSR (simple sequence repeats) to detect the molecular effects of water pollutants on O. niloticus, molecular markers indicated genetic differences in fi...

Research paper thumbnail of Heavy Metals and Macronutrients Concentration in Oreochromis Njloticus and Tilapia Zilliifish Species Inhabiting Some Egyptian Lakes and El-Salam Canal

Egyptian Journal of Aquatic Biology and Fisheries, 2005

T he present study was initiated to assess the levels of Fe, Mn, Zn. Cu. Ni. Co. Pb and Cd togeth... more T he present study was initiated to assess the levels of Fe, Mn, Zn. Cu. Ni. Co. Pb and Cd together with Na, K, Ca and Mg in liver and muscle of Tilapia zillii from three different aquatic ecosystems namely. Lake Manzaiah (Brackish water). Lake Qarun (Saline water) and El-Salam Canal (fresh water) and Oreochromis niloticus from Lake Manzaiah and El-Salam Canal throughout four seasons (1999-2H00). The present results revealed that liver has higher tendency to accumulate Fe and Cu more than the other studied heavy metals for O. niloticus and T. zillii in different studied sites. Zn followed Fe in the accumulation abundance in muscle tissues of the two studied species and Cd was the lowest one. The fluctuation of metal concentrations in muscle tissues for O. niloticus and T. zillii exhibited the following descending order Fe>Zn>Mn=Cu-Ni=Co=Pb>Cd. while the abundance order of the same metals in liver tissues was: Fe>Cu>Zn>Mn>Ni>Co= Pb>Cd.

Research paper thumbnail of Heavy Lmetals Accumulation and Macronutrients in the Livers of Some Fish Species of Bardawil Lagoon and Their Histological Changes

Egyptian Journal of Aquatic Biology and Fisheries, 2003

F ish samples of Solea vulgaris. Mugil cephalus, Liza ramada and Spams aurata were collected duri... more F ish samples of Solea vulgaris. Mugil cephalus, Liza ramada and Spams aurata were collected during November 2001 from El Telol location in Bardawil lagoon, north eastern of Egyptian Mediterranean coast. The study was conducted to assess the effect of the heavy metals (Fe. Mn. Zm Cm Pb and Cd) and the macro nutrients (Na, K, Ca, and Mg) on livers tissues of the four fish species. The results showed that the concentrations of Fe, Mn, Zn, Cu, Pb, and Cd in the livers of the four fish species ranged between 48.

Research paper thumbnail of Pathological changes in liver structure and function of Oreochromis niloticus experimentally exposed to Escherichia coli

International Journal of Biotechnology and Bioengineering, 2017

Authman et al. (2013) noticed increase in glutamate-oxaloacetate transaminase (GOT) and glutamate... more Authman et al. (2013) noticed increase in glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities and different biochemical parameters of clarias gariepinus living in El-Rahawy drain. Also, histological study cleared that liver of C. gariepinus living in El-Rahawy drain suffered from several pathological alterations. All these findings were discussed and referred to the input of agricultural, industrial and sewage water into El-Rahawy drain.

Research paper thumbnail of Accumulation of heavy metals in tilapia fish species and related histopathological changes in muscles, gills and liver of Oreochromis niloticus occurring in the area of Qahr El-Bahr, Lake Al-Manzalah, Egypt

Oceanological and Hydrobiological Studies, 2021

Accumulation of metals (Fe, Zn, Cu, Mn, Pb, Cd and Ni) in the muscles and gills of Oreochromis ni... more Accumulation of metals (Fe, Zn, Cu, Mn, Pb, Cd and Ni) in the muscles and gills of Oreochromis niloticus, Oreochromis aureus and Tilapia zillii was assessed based on seven locations in the Qahr El-Bahr area in Lake Al-Manzalah. The average accumulation of metals was in the following order: Tilapia zillii > Oreochromis aureus > Oreochromis niloticus. To determine the risk of fish consumption, the estimated daily intake, carcinogenic, non-carcinogenic and relative risk indices were calculated. The results indicate that the intake of individual metals through the consumption of fish is almost safe for human health, whereas the intake of combined metals poses a high potential health risk to consumers. Assessment of carcinogenic risk of Cd and Ni from the consumption of tilapia species indicates that consumers are at risk of cancer. The contribution of Pb and Cd to the overall relative risk index ranged from 34% to 41%. Of the three species studied, Oreochromis niloticus is relativ...

Research paper thumbnail of Injuries in Kidney Tissue of the Mugil Fish Liza Ramada as a Biomarker for Environmental Pollution in the Lake Manzalah

Egyptian Journal of Aquatic Biology and Fisheries, 2003

T his study deals with the effect of aquatic environmental pollution of Lake Manzalah on Lizarama... more T his study deals with the effect of aquatic environmental pollution of Lake Manzalah on Lizaramada. The fish and water samples were collected seasonally for a year from Autumn 2000 to Summer 2001 from the selected stations near Boughaz El-Gamil. Other water samples were collected from 12 different stations in other locations. The study included heavy metal analysis of the water lake and histopathological changes in the kidneys of the same fish. The investigation revealed that the high values of heavy metals (Fe, Mn, Cu, Zn, Pb and Cd) encountered during the different seasons in lake Manzalah induced toxic effects on Lizaramada kidneys which may decrease the productivity of this Mugil species. So, it is recommended to try to protect this fish from the destruction by drainage water loaded with agricultural and industrial pollutants as well as sewage. 0 Shadia M. Kadry et ah considered as a sink for disposing industrial and human wastes. A total amount of about 7500 million cubic meters of untreated industrial domestic and drainage water as well as agrochemicals (fertilizers and biocides) is discharged annually into the lake through seven main drains (Ibrahim etal, 1997). Hadous, Ramsis and Bahr El-Bakar drains contribute about 75% of the total annual drainage water into the southern sector of the lake. Previous studies on the hydrography and chemical properties of Lake Manzalah have been reported by El Wakeel and Wahby (1970) and Wahby et al (1972). They concluded that the drainage water changed the nutrient level of the lake in many respects especially the nitrate phosphate ratio. The residue levels of petroleum hydrocarbons, chlorinated insecticides, polychlorinated biphenyls and organochlorine pesticides in lake Manzalah were studied by Abou-Arab et al (1995) and Badawye/tf/. (1995). Abdel-Satar (2001) studied the effect of drains on the southern of lake Manzalah. The study revealed depletion of dissolved oxygen and high concentrations of nutrients, chemical oxygen demand, biochemical oxygen demand and heavy metals. As an indicator of exposure to contaminants, histology represents a useful tool to assess the degree of pollution (Bernet et al, 1999). The kidney of fish is a suitable organ for histological examination in order to determine the effect of pollution (Bucher and Hofer, 1993). Many authors reported histopathological abnormalities in kidney of different fish species that were exposed to several pollutants (agricultural, industrial and sewage) (Daskalow etal t 2000 and Lliopoulou & Kotsani, 2001). The present study is initiated as a framework to seek an overview of the impact of water pollution in lake Manzalah on the histopathological changes in Liza ramada kidney. In order to proceed to asound assessment of this effect, it was first necessary to monitor the levels of heavy metals pollution in the lake throughout the year by four seasons. MATERIAL AND METHODS A-Collection And Preparation of Water Samples: Water samples were collected seasonally from Autumn 2000 to Sximmer 2001. Twelve stations were selected to cover the area of the lake and divided into four sectors. The location of sampling stations are presented in Fig(l). The samples were collected from