Aman Shah Abdul Majid - Academia.edu (original) (raw)
Papers by Aman Shah Abdul Majid
The Open Conference Proceedings Journal, 2013
Water based plant extract powders are commonly used for various medicinal applications due to the... more Water based plant extract powders are commonly used for various medicinal applications due to their simple method of extraction. These extract powders are usually hygroscopic which make them difficult to handle especially in high humidity environment. Hence to further develop them into various pharmaceutical formulations becomes a challenge. This work looks into hygroscopic extract formulation in granular form which can reduce moisture content and increase flow. Water based leaf extract of Orthosiphon Stamineus (Benth) was selected for this study. Extract powder and formulated granules were stored at 30 0 C/65% and 40 0 C/75% RH for three months. Influence of excipients on formulation was tested in terms of moisture content, color, bulk density, tap density, compression index, and flow properties. Formulation with Magnesium Aluminometasilicate reduced the moisture content and increase the flow during and after storage for predetermined time. Where as other selected excipients decrease moisture content without improving the flow. The findings provide an insight into a possible approach for formulating moisture-sensitive pharmaceutical products, especially dry powder preparations for use in high humidity environment.
Journal of Coordination Chemistry
Computational Biology and Chemistry
European Journal of Integrative Medicine
Reviews in Inorganic Chemistry
Technetium radionuclide (99mTc) has excellent extent of disintegration properties and occupies a ... more Technetium radionuclide (99mTc) has excellent extent of disintegration properties and occupies a special place in the field of nuclear medicinal chemistry and other health disciplines. Current review describes recent approaches of synthesis in detailed ways for radio-pharmaceuticals of technetium which have been developed to treat and diagnose the biotic disorders. These technetium labeled radio-pharmaceuticals have been established to apply in the field of diagnostic nuclear medicine especially for imaging of different body parts such as brain, heart, kidney, bones and so on, through single photon emission computed tomography (SPECT) that is thought to be difficult to image such organs by using common X-ray and MRI (Magnetic Resonance Imaging) techniques. This review highlights and accounts an inclusive study on the various synthetic routes of technetium labeled radio-pharmaceuticals using ligands with various donor atoms such as carbon, nitrogen, sulphur, phosphorus etc. These com...
Advanced Bioinformatics and Chemistry
Advanced Bioinformatics and Chemistry
Journal of Angiotherapy
In this study, we aimed to evaluate the efficacy and safety administration of Labeesity® 125mg, a... more In this study, we aimed to evaluate the efficacy and safety administration of Labeesity® 125mg, an anti-obesity natural product supplement available in the market. A clinical obese female with medication-controlled hypothyroidism volunteered for this trial. The patient was supplemented with Labeesity® 125mg twice daily for 40 days. The hematological, serum biochemical, subject characteristic were recorded before and after treatment. When compared with the baseline reading, the treatment was favorable for weight loss, with hip and waist circumference reduction. It also improved the patient's general quality of life over the course of 40 days. There was no treatment related negative clinical symptoms except for mild bloating which dissipated after the second week of supplementation. Furthermore, there were no significant hematological and biochemical alterations, except for a slight fluctuation in uric acid. Labeesity® 125mg intake for the volunteer is assumed to be generally safe with moderate improvement in weight reduction, body composition and serum lipid profile. Longer period of observations on the effects of Labeesity 125mg would be required to evaluate if the positive effects seen would persist beyond 40 days of intervention. Therefore, strong evidence to support the potential effectiveness and long-term benefits of Labisia pumila extracts based products will require robust studies to be conducted utilizing larger patient numbers and over extended periods of time to rule out any potential toxic effects.
Journal of Angiotherapy
Glioblastoma (GBM) is a highly angiogenic malignant primary brain tumor with very poor patient su... more Glioblastoma (GBM) is a highly angiogenic malignant primary brain tumor with very poor patient survival rates. Here, we present Rosmarinic acid and its new salt and base derivatives that show potential anti-glioblastoma (anti-GBM) activity via disruption of IL17A-mediated downstream angiogenesis pathway. These new compounds were rationally designed, synthesized and the oral CNS physicochemical properties were determined. The anti-GBM activity of the compounds were determined using cell migration and proliferation assays in U87 MG, DBTRG MG and EA.hy926 cells. The compounds activity on IL17A and VEGF expression was determined using ELISA and apoptotic activity was measured by caspase assays. The compounds showed stability in serum and at different pH similar to Rosmarinic acid stoichiometry. Moreover, the salt derivatives were highly hydrophilic and bases were highly lipophilic. These derivatives showed blood-brain barrier permeability >2 fold more than rosmarinic acid (P<0.001). ROS were differentially expressed after treatment with salt and base rosmarinates in U87 MG (P<0.0001). The compounds showed cytostatic and anti-angiogenic activity. The IC50 of the silver salts was found to be > 1200 µg/ml thus excluding them from being classed as toxic compounds. In conclusion, metal salts and base rosmarinates demonstrated potent anti-GBM efficacy as IL17A inhibitor with minimal toxicity.
Australian herbal Insight
Metabolic Syndrome is a collection of associated health problems that affects much of the world p... more Metabolic Syndrome is a collection of associated health problems that affects much of the world population today. These include hypertension, obesity, hypercholesterolemia, and insulin resistance; all of which can increase the risk of stroke, heart disease, and type 2 diabetes. These illnesses are interrelated and having one complication can lead to several other diseases. A number of herbs that have high antioxidant and anti-inflammatory properties can help to improve the management of metabolic syndrome. Orthosiphon stamineus, also known commonly as Cat Whiskers herb is widely used in the Asian region to treat metabolic syndrome associated health problems. This is due to its unique phytochemical properties that target key biochemical pathways that are involved in metabolic diseases. This review highlights key aspects of Metabolic Syndrome and how Cat Whiskers herb can play an important role in treating this disease.
Biomedicine & Pharmacotherapy
Proven the great potential of essential oils as anticancer agents, the current study intended to ... more Proven the great potential of essential oils as anticancer agents, the current study intended to explore molecular mechanisms responsible for in vitro and in vivo anti-colon cancer efficacy of essential oil containing oleo-gum resin extract (RH) of Mesua ferrea. MTT cell viability studies showed that RH had broad spectrum cytotoxic activities. However, it induced more profound growth inhibitory effects towards two human colon cancer cell lines i.e., HCT 116 and LIM1215 with an IC values of 17.38 ± 0.92 and 18.86 ± 0.80 μg/mL respectively. RH induced relatively less toxicity in normal human colon fibroblasts i.e., CCD-18co. Cell death studies conducted, revealed that RH induced characteristic morphological and biochemical changes in HCT 116. At protein level it down-regulated expression of multiple pro-survival proteins i.e., survivin, xIAP, HSP27, HSP60 and HSP70 and up-regulated expression of ROS, caspase-3/7 and TRAIL-R2 in HCT 116. Furthermore, significant reduction in invasion, migration and colony formation potential was observed in HCT 116 treated with RH. Chemical characterization by GC-MS and HPLC methods revealed isoledene and elemene as one the major compounds. RH showed potent antitumor activity in xenograft model. Overall, these findings suggest that RH holds a promise to be further studied for cheap anti-colon cancer naturaceutical development.
American journal of translational research, 2017
The process of wound healing involves activation of keratinocytes, fibroblasts, endothelial cells... more The process of wound healing involves activation of keratinocytes, fibroblasts, endothelial cells, etc. Angiogenesis is crucial during the process of wound healing. Virgin coconut oil is widely utilized in South Asia for various purposes including food, medicinal and industrial applications. This study aimed to evaluate the potency of fermented virgin coconut oil (FVCO) in angiogenesis and wound healing via both in vitro and in vivo assays. Human umbilical vein endothelial (HUVEC), fibroblast (CCD-18) and retinal ganglion (RGC-5) cells were cultured in medium containing different concentrations of FVCO. The proliferation, migration and morphological changes of cells were determined. The angiogenic effect of FVCO was evaluated by rat aortic assay. The therapeutic effect of FVCO on wound healing was further assessed in a wound excision model in Sprague Dawley rats. The expression of phospho-VEGFR2 (vascular endothelial growth factor receptor 2) in HUVECs was detected by Western blot. ...
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Jan 24, 2017
The aim of the present study is to investigate the effect of long non-coding RNA-MALAT1 (LncRNA-M... more The aim of the present study is to investigate the effect of long non-coding RNA-MALAT1 (LncRNA-MALAT1) on retinal ganglion cell (RGC) apoptosis mediated by the PI3K/Akt signaling pathway in rats with glaucoma. RGCs were isolated and cultured, and monoclonal antibodies (anti-rat Thy-1, Brn3a and RBPMS) were examined by immunocytochemistry. An overexpression vector MALAT1-RNA activation (RNAa), gene knockout vector MALAT1-RNA interference (RNAi), and control vector MALAT1-negative control (NC) were constructed. A chronic high intraocular pressure (IOP) rat model of glaucoma was established by episcleral vein cauterization. The RGCs were divided into the RGC control, RGC pressure, RGC pressure + MALAT1-NC, RGC pressure + MALAT1-RNAi and RGC pressure + MALAT1-RNAa groups. Sixty Sprague-Dawley (SD) rats were randomly divided into the normal, high IOP, high IOP + MALAT1-NC, high IOP + MALAT1-RNAa and high IOP + MALAT1-RNAi groups. qRT-PCR and western blotting were used to detect the expr...
Journal of Herbal Medicine
Chinese journal of natural medicines, 2017
Considering the great potential of natural products as anticancer agents, the present study was d... more Considering the great potential of natural products as anticancer agents, the present study was designed to explore the molecular mechanisms responsible for anticancer activities of Mesua ferrea stem bark extract against human colorectal carcinoma. Based on MTT assay results, bioactive sub-fraction (SF-3) was selected for further studies using HCT 116 cells. Repeated column chromatography resulted in isolation of less active α-amyrin from SF-3, which was identified and characterized by GC-MS and HPLC methods. α-amyrin and betulinic acid contents of SF-3 were measured by HPLC methods. Fluorescent assays revealed characteristic apoptotic features, including cell shrinkage, nuclear condensation, and marked decrease in mitochondrial membrane potential in SF-3 treated cells. In addition, increased levels of caspases-9 and -3/7 levels were also observed in SF-3 treated cells. SF-3 showed promising antimetastatic properties in multiple in vitro assays. Multi-pathway analysis revealed signi...
Drug and chemical toxicology, Jan 21, 2017
Herbal products contain a variety of compounds which may be useful in protecting against cellular... more Herbal products contain a variety of compounds which may be useful in protecting against cellular damage caused by mutagens. Orthosiphon stamineus (O.s) also known as Cat whiskers. The herb has been shown anti-oxidative properties and can modulate key cellular proteins that have cytoprotective effect. The study aimed to evaluate the effects of different doses (250, 500 and 1000 mg kg(-1)) of 50% ethanol extract of O.s (Et. O.s) on micro-nucleated polychromatic erythrocytes (MNPCE), Polychromatic to normachromatic erythrocytes ratio (PCE/NCE), Mitotic index (MI), and Chromosomal aberration (CA) in Bab/c mice. Moreover, these parameters were used to evaluate the anti-genotoxic and clastogenic potencies of (Et. O.s) against mitomycin c (MMC) that interact with biological molecules and induce genotoxic and clastogenic disorders in non-tumor cells. MMC (4 mg kg(-1)) was injected intraperitoneally (i.p.) to the mice before and after treatment with three different doses of (Et. O.s). The r...
European Journal of Pharmacology, 2017
Despite many treatment options, cancer remains a growing problem and has become the second leadin... more Despite many treatment options, cancer remains a growing problem and has become the second leading cause of death globally. Here, we present fluorescence molecular tomography (FMT) data regarding the reversion of third generation co-cultured U87+DBTRG and patient-derived GBM tumor model after treatment with novel IL17A inhibitor named FLVM and FLVZ (organic derivatives of caffeic acid). FMT was used to determine tumor angiogenesis volume (assessment of number of blood vessel; the expression of angiogenic factors CD34 and other angiogenic cancer bio-markers) in U87+DBTRG and patient-derived gliomas. Immunohistochemistry was used to determine microvessel density [CD34], and cell proliferation [Ki67]. Western blot was used to assess the interleukin 17A [IL17A], vascular endothelial growth factor [VEGF] and hypoxia-inducible factor-1α [HIF-1α]. Antibody array was used to assess the cancer bio-markers in co-cultured U87+DBTRG gliomas. Animal survival was found to be significantly increased (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.0001) after FLVM treatment compared with control-IL17A. After FMT detection, FLVM, administered orally, was found to decrease tumor growth (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.0001). FLVM and FLVZ administration resulted in significant decreases in tumor hypoxia [HIF-1α (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05)], angiogenesis [CD34 (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05)], VEGF, IL17A and cell proliferation [Ki67 (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05)] and caused a significant increase of Bax, caspase and FasL (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05), compared with untreated animals. Additionally, Leptin, LPL (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.01), FFA…
International Journal of Phytomedicine, 2017
Syzygium campanulatum consumed medicinal herb and rich in phytochemical content and notable antia... more Syzygium campanulatum consumed medicinal herb and rich in phytochemical content and notable antiangiogenic and anti In this s FTIR and HPLC, phytochemicals content and repeated doses toxicity and mutagenicity effect of the hydroethanolic extract of experimental and control groups and fed with 500, 1000, 2000 mg/kg/day of and 90 days and with a single dose of 5000 mg/kg in acute study. The obtained results showed meta betulinic acid (60.43 mg/g.), total glycol saponins, total phenolics, total proteins, total tannins, and total flavonoids. No mutagenicity was detected in of clinical toxicity and mortality were observed in the experimental groups after 28 and 90 experiment. However, significant (p< 0.05) hematological, and biochemical parameters but they were with rat, therefore not considered treatment Based on these findings the fifty percent lethal dose up to 2000 mg/kg for 90 days, as such Keywords Subchronic toxicity. This article is distributed under the terms of the and redistribution provided that the origina
Journal of the Neurological Sciences, 2016
Optic neuropathy is a neurodegenerative disease which involves optic nerve injury. It is caused b... more Optic neuropathy is a neurodegenerative disease which involves optic nerve injury. It is caused by acute or intermittent insults leading to visual dysfunction. There are number of factors, responsible for optic neuropathy, and the optic nerve axon is affected in all type which causes the loss of retinal ganglion cells. In this review we will highlight various mechanisms involved in the cell loss cascades during axonal degeneration as well as ischemic optic neuropathy. These mechanisms include oxidative stress, excitotoxicity, angiogenesis, neuroinflammation and apoptosis following retinal ischemia. We will also discuss the effect of neuroprotective agents in attenuation of the negative effect of factors involve in the disease occurrence and progression.
The Open Conference Proceedings Journal, 2013
Water based plant extract powders are commonly used for various medicinal applications due to the... more Water based plant extract powders are commonly used for various medicinal applications due to their simple method of extraction. These extract powders are usually hygroscopic which make them difficult to handle especially in high humidity environment. Hence to further develop them into various pharmaceutical formulations becomes a challenge. This work looks into hygroscopic extract formulation in granular form which can reduce moisture content and increase flow. Water based leaf extract of Orthosiphon Stamineus (Benth) was selected for this study. Extract powder and formulated granules were stored at 30 0 C/65% and 40 0 C/75% RH for three months. Influence of excipients on formulation was tested in terms of moisture content, color, bulk density, tap density, compression index, and flow properties. Formulation with Magnesium Aluminometasilicate reduced the moisture content and increase the flow during and after storage for predetermined time. Where as other selected excipients decrease moisture content without improving the flow. The findings provide an insight into a possible approach for formulating moisture-sensitive pharmaceutical products, especially dry powder preparations for use in high humidity environment.
Journal of Coordination Chemistry
Computational Biology and Chemistry
European Journal of Integrative Medicine
Reviews in Inorganic Chemistry
Technetium radionuclide (99mTc) has excellent extent of disintegration properties and occupies a ... more Technetium radionuclide (99mTc) has excellent extent of disintegration properties and occupies a special place in the field of nuclear medicinal chemistry and other health disciplines. Current review describes recent approaches of synthesis in detailed ways for radio-pharmaceuticals of technetium which have been developed to treat and diagnose the biotic disorders. These technetium labeled radio-pharmaceuticals have been established to apply in the field of diagnostic nuclear medicine especially for imaging of different body parts such as brain, heart, kidney, bones and so on, through single photon emission computed tomography (SPECT) that is thought to be difficult to image such organs by using common X-ray and MRI (Magnetic Resonance Imaging) techniques. This review highlights and accounts an inclusive study on the various synthetic routes of technetium labeled radio-pharmaceuticals using ligands with various donor atoms such as carbon, nitrogen, sulphur, phosphorus etc. These com...
Advanced Bioinformatics and Chemistry
Advanced Bioinformatics and Chemistry
Journal of Angiotherapy
In this study, we aimed to evaluate the efficacy and safety administration of Labeesity® 125mg, a... more In this study, we aimed to evaluate the efficacy and safety administration of Labeesity® 125mg, an anti-obesity natural product supplement available in the market. A clinical obese female with medication-controlled hypothyroidism volunteered for this trial. The patient was supplemented with Labeesity® 125mg twice daily for 40 days. The hematological, serum biochemical, subject characteristic were recorded before and after treatment. When compared with the baseline reading, the treatment was favorable for weight loss, with hip and waist circumference reduction. It also improved the patient's general quality of life over the course of 40 days. There was no treatment related negative clinical symptoms except for mild bloating which dissipated after the second week of supplementation. Furthermore, there were no significant hematological and biochemical alterations, except for a slight fluctuation in uric acid. Labeesity® 125mg intake for the volunteer is assumed to be generally safe with moderate improvement in weight reduction, body composition and serum lipid profile. Longer period of observations on the effects of Labeesity 125mg would be required to evaluate if the positive effects seen would persist beyond 40 days of intervention. Therefore, strong evidence to support the potential effectiveness and long-term benefits of Labisia pumila extracts based products will require robust studies to be conducted utilizing larger patient numbers and over extended periods of time to rule out any potential toxic effects.
Journal of Angiotherapy
Glioblastoma (GBM) is a highly angiogenic malignant primary brain tumor with very poor patient su... more Glioblastoma (GBM) is a highly angiogenic malignant primary brain tumor with very poor patient survival rates. Here, we present Rosmarinic acid and its new salt and base derivatives that show potential anti-glioblastoma (anti-GBM) activity via disruption of IL17A-mediated downstream angiogenesis pathway. These new compounds were rationally designed, synthesized and the oral CNS physicochemical properties were determined. The anti-GBM activity of the compounds were determined using cell migration and proliferation assays in U87 MG, DBTRG MG and EA.hy926 cells. The compounds activity on IL17A and VEGF expression was determined using ELISA and apoptotic activity was measured by caspase assays. The compounds showed stability in serum and at different pH similar to Rosmarinic acid stoichiometry. Moreover, the salt derivatives were highly hydrophilic and bases were highly lipophilic. These derivatives showed blood-brain barrier permeability >2 fold more than rosmarinic acid (P<0.001). ROS were differentially expressed after treatment with salt and base rosmarinates in U87 MG (P<0.0001). The compounds showed cytostatic and anti-angiogenic activity. The IC50 of the silver salts was found to be > 1200 µg/ml thus excluding them from being classed as toxic compounds. In conclusion, metal salts and base rosmarinates demonstrated potent anti-GBM efficacy as IL17A inhibitor with minimal toxicity.
Australian herbal Insight
Metabolic Syndrome is a collection of associated health problems that affects much of the world p... more Metabolic Syndrome is a collection of associated health problems that affects much of the world population today. These include hypertension, obesity, hypercholesterolemia, and insulin resistance; all of which can increase the risk of stroke, heart disease, and type 2 diabetes. These illnesses are interrelated and having one complication can lead to several other diseases. A number of herbs that have high antioxidant and anti-inflammatory properties can help to improve the management of metabolic syndrome. Orthosiphon stamineus, also known commonly as Cat Whiskers herb is widely used in the Asian region to treat metabolic syndrome associated health problems. This is due to its unique phytochemical properties that target key biochemical pathways that are involved in metabolic diseases. This review highlights key aspects of Metabolic Syndrome and how Cat Whiskers herb can play an important role in treating this disease.
Biomedicine & Pharmacotherapy
Proven the great potential of essential oils as anticancer agents, the current study intended to ... more Proven the great potential of essential oils as anticancer agents, the current study intended to explore molecular mechanisms responsible for in vitro and in vivo anti-colon cancer efficacy of essential oil containing oleo-gum resin extract (RH) of Mesua ferrea. MTT cell viability studies showed that RH had broad spectrum cytotoxic activities. However, it induced more profound growth inhibitory effects towards two human colon cancer cell lines i.e., HCT 116 and LIM1215 with an IC values of 17.38 ± 0.92 and 18.86 ± 0.80 μg/mL respectively. RH induced relatively less toxicity in normal human colon fibroblasts i.e., CCD-18co. Cell death studies conducted, revealed that RH induced characteristic morphological and biochemical changes in HCT 116. At protein level it down-regulated expression of multiple pro-survival proteins i.e., survivin, xIAP, HSP27, HSP60 and HSP70 and up-regulated expression of ROS, caspase-3/7 and TRAIL-R2 in HCT 116. Furthermore, significant reduction in invasion, migration and colony formation potential was observed in HCT 116 treated with RH. Chemical characterization by GC-MS and HPLC methods revealed isoledene and elemene as one the major compounds. RH showed potent antitumor activity in xenograft model. Overall, these findings suggest that RH holds a promise to be further studied for cheap anti-colon cancer naturaceutical development.
American journal of translational research, 2017
The process of wound healing involves activation of keratinocytes, fibroblasts, endothelial cells... more The process of wound healing involves activation of keratinocytes, fibroblasts, endothelial cells, etc. Angiogenesis is crucial during the process of wound healing. Virgin coconut oil is widely utilized in South Asia for various purposes including food, medicinal and industrial applications. This study aimed to evaluate the potency of fermented virgin coconut oil (FVCO) in angiogenesis and wound healing via both in vitro and in vivo assays. Human umbilical vein endothelial (HUVEC), fibroblast (CCD-18) and retinal ganglion (RGC-5) cells were cultured in medium containing different concentrations of FVCO. The proliferation, migration and morphological changes of cells were determined. The angiogenic effect of FVCO was evaluated by rat aortic assay. The therapeutic effect of FVCO on wound healing was further assessed in a wound excision model in Sprague Dawley rats. The expression of phospho-VEGFR2 (vascular endothelial growth factor receptor 2) in HUVECs was detected by Western blot. ...
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Jan 24, 2017
The aim of the present study is to investigate the effect of long non-coding RNA-MALAT1 (LncRNA-M... more The aim of the present study is to investigate the effect of long non-coding RNA-MALAT1 (LncRNA-MALAT1) on retinal ganglion cell (RGC) apoptosis mediated by the PI3K/Akt signaling pathway in rats with glaucoma. RGCs were isolated and cultured, and monoclonal antibodies (anti-rat Thy-1, Brn3a and RBPMS) were examined by immunocytochemistry. An overexpression vector MALAT1-RNA activation (RNAa), gene knockout vector MALAT1-RNA interference (RNAi), and control vector MALAT1-negative control (NC) were constructed. A chronic high intraocular pressure (IOP) rat model of glaucoma was established by episcleral vein cauterization. The RGCs were divided into the RGC control, RGC pressure, RGC pressure + MALAT1-NC, RGC pressure + MALAT1-RNAi and RGC pressure + MALAT1-RNAa groups. Sixty Sprague-Dawley (SD) rats were randomly divided into the normal, high IOP, high IOP + MALAT1-NC, high IOP + MALAT1-RNAa and high IOP + MALAT1-RNAi groups. qRT-PCR and western blotting were used to detect the expr...
Journal of Herbal Medicine
Chinese journal of natural medicines, 2017
Considering the great potential of natural products as anticancer agents, the present study was d... more Considering the great potential of natural products as anticancer agents, the present study was designed to explore the molecular mechanisms responsible for anticancer activities of Mesua ferrea stem bark extract against human colorectal carcinoma. Based on MTT assay results, bioactive sub-fraction (SF-3) was selected for further studies using HCT 116 cells. Repeated column chromatography resulted in isolation of less active α-amyrin from SF-3, which was identified and characterized by GC-MS and HPLC methods. α-amyrin and betulinic acid contents of SF-3 were measured by HPLC methods. Fluorescent assays revealed characteristic apoptotic features, including cell shrinkage, nuclear condensation, and marked decrease in mitochondrial membrane potential in SF-3 treated cells. In addition, increased levels of caspases-9 and -3/7 levels were also observed in SF-3 treated cells. SF-3 showed promising antimetastatic properties in multiple in vitro assays. Multi-pathway analysis revealed signi...
Drug and chemical toxicology, Jan 21, 2017
Herbal products contain a variety of compounds which may be useful in protecting against cellular... more Herbal products contain a variety of compounds which may be useful in protecting against cellular damage caused by mutagens. Orthosiphon stamineus (O.s) also known as Cat whiskers. The herb has been shown anti-oxidative properties and can modulate key cellular proteins that have cytoprotective effect. The study aimed to evaluate the effects of different doses (250, 500 and 1000 mg kg(-1)) of 50% ethanol extract of O.s (Et. O.s) on micro-nucleated polychromatic erythrocytes (MNPCE), Polychromatic to normachromatic erythrocytes ratio (PCE/NCE), Mitotic index (MI), and Chromosomal aberration (CA) in Bab/c mice. Moreover, these parameters were used to evaluate the anti-genotoxic and clastogenic potencies of (Et. O.s) against mitomycin c (MMC) that interact with biological molecules and induce genotoxic and clastogenic disorders in non-tumor cells. MMC (4 mg kg(-1)) was injected intraperitoneally (i.p.) to the mice before and after treatment with three different doses of (Et. O.s). The r...
European Journal of Pharmacology, 2017
Despite many treatment options, cancer remains a growing problem and has become the second leadin... more Despite many treatment options, cancer remains a growing problem and has become the second leading cause of death globally. Here, we present fluorescence molecular tomography (FMT) data regarding the reversion of third generation co-cultured U87+DBTRG and patient-derived GBM tumor model after treatment with novel IL17A inhibitor named FLVM and FLVZ (organic derivatives of caffeic acid). FMT was used to determine tumor angiogenesis volume (assessment of number of blood vessel; the expression of angiogenic factors CD34 and other angiogenic cancer bio-markers) in U87+DBTRG and patient-derived gliomas. Immunohistochemistry was used to determine microvessel density [CD34], and cell proliferation [Ki67]. Western blot was used to assess the interleukin 17A [IL17A], vascular endothelial growth factor [VEGF] and hypoxia-inducible factor-1α [HIF-1α]. Antibody array was used to assess the cancer bio-markers in co-cultured U87+DBTRG gliomas. Animal survival was found to be significantly increased (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.0001) after FLVM treatment compared with control-IL17A. After FMT detection, FLVM, administered orally, was found to decrease tumor growth (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.0001). FLVM and FLVZ administration resulted in significant decreases in tumor hypoxia [HIF-1α (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05)], angiogenesis [CD34 (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05)], VEGF, IL17A and cell proliferation [Ki67 (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05)] and caused a significant increase of Bax, caspase and FasL (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05), compared with untreated animals. Additionally, Leptin, LPL (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.01), FFA…
International Journal of Phytomedicine, 2017
Syzygium campanulatum consumed medicinal herb and rich in phytochemical content and notable antia... more Syzygium campanulatum consumed medicinal herb and rich in phytochemical content and notable antiangiogenic and anti In this s FTIR and HPLC, phytochemicals content and repeated doses toxicity and mutagenicity effect of the hydroethanolic extract of experimental and control groups and fed with 500, 1000, 2000 mg/kg/day of and 90 days and with a single dose of 5000 mg/kg in acute study. The obtained results showed meta betulinic acid (60.43 mg/g.), total glycol saponins, total phenolics, total proteins, total tannins, and total flavonoids. No mutagenicity was detected in of clinical toxicity and mortality were observed in the experimental groups after 28 and 90 experiment. However, significant (p< 0.05) hematological, and biochemical parameters but they were with rat, therefore not considered treatment Based on these findings the fifty percent lethal dose up to 2000 mg/kg for 90 days, as such Keywords Subchronic toxicity. This article is distributed under the terms of the and redistribution provided that the origina
Journal of the Neurological Sciences, 2016
Optic neuropathy is a neurodegenerative disease which involves optic nerve injury. It is caused b... more Optic neuropathy is a neurodegenerative disease which involves optic nerve injury. It is caused by acute or intermittent insults leading to visual dysfunction. There are number of factors, responsible for optic neuropathy, and the optic nerve axon is affected in all type which causes the loss of retinal ganglion cells. In this review we will highlight various mechanisms involved in the cell loss cascades during axonal degeneration as well as ischemic optic neuropathy. These mechanisms include oxidative stress, excitotoxicity, angiogenesis, neuroinflammation and apoptosis following retinal ischemia. We will also discuss the effect of neuroprotective agents in attenuation of the negative effect of factors involve in the disease occurrence and progression.