Amanda Wright - Academia.edu (original) (raw)
Papers by Amanda Wright
Scientific Reports, 2021
We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers wi... more We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical axis. This is achieved without the need for a high precision z-stage, separate calibration sample, nor a priori knowledge of either the bead size or the optical properties of the suspending medium. Instead, we have developed a simple yet effective in situ spatial calibration method using image sharpness and exploiting the fact we image at multiple planes simultaneously. These features make OpTIMuM an ideal system for microrheology measurements, and we corroborate the effectiveness of this novel microrheology tool by measuring the viscosity of water in three dimensions, simultaneously.
Applied Physics Letters
Page 1. Mechanisms of optical angular momentum transfer to nematic liquid crystalline droplets Ti... more Page 1. Mechanisms of optical angular momentum transfer to nematic liquid crystalline droplets Tiffany A. Wood, Helen F. Gleeson,a) Mark R. Dickinson, and Amanda J. Wright Department of Physics and Astronomy, University ...
Optics Express, 2006
We report the creation of white-light, achromatic Bessel beams using a spatial light modulator an... more We report the creation of white-light, achromatic Bessel beams using a spatial light modulator and a prism to compensate for the dispersion. Unlike the Bessel beam created by a refractive axicon, this achromatic beam has a radial wavevector and hence an intensity cross-section which is independent of wavelength. The technique also lends itself to the generation of higher order Bessel
Lab on A Chip, 2005
We report the creation of permanent 3D configurations of cells, at predefined positions, within a... more We report the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.
Journal of Environmental Medicine, 2005
We report on the introduction of active optical elements into confocal and multiphoton microscope... more We report on the introduction of active optical elements into confocal and multiphoton microscopes in order to reduce the sample-induced aberration. Using a flexible membrane mirror as the active element, the beam entering the rear of the microscope objective is altered to produce the smallest point spread function once it is brought to a focus inside the sample. The conventional approach to adaptive optics, commonly used in astronomy, is to utilise a wavefront sensor to determine the required mirror shape. We have developed a technique that uses optimisation algorithms to improve the returned signal without the use of a wavefront sensor. We have investigated a number of possible optimisation methods, covering hill climbing, genetic algorithms, and more random search methods. The system has demonstrated a significant enhancement in the axial resolution of a confocal microscope when imaging at depth within a sample. We discuss the trade-offs of the various approaches adopted, comparing speed with resolution enhancement.
Optical Trapping and Optical Micromanipulation VIII, 2011
We present results demonstrating for the first time that an optically trapped bead can be used as... more We present results demonstrating for the first time that an optically trapped bead can be used as a local probe to measure the variation in the viscoelastic properties of the vitreous humor of a rabbit eye. The Brownian motion of the optically trapped bead was monitored on a fast CCD camera on the millisecond timescale. Analysis of the bead trajectory
Scientific Reports, 2021
We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers wi... more We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical axis. This is achieved without the need for a high precision z-stage, separate calibration sample, nor a priori knowledge of either the bead size or the optical properties of the suspending medium. Instead, we have developed a simple yet effective in situ spatial calibration method using image sharpness and exploiting the fact we image at multiple planes simultaneously. These features make OpTIMuM an ideal system for microrheology measurements, and we corroborate the effectiveness of this novel microrheology tool by measuring the viscosity of water in three dimensions, simultaneously.
Applied Physics Letters
Page 1. Mechanisms of optical angular momentum transfer to nematic liquid crystalline droplets Ti... more Page 1. Mechanisms of optical angular momentum transfer to nematic liquid crystalline droplets Tiffany A. Wood, Helen F. Gleeson,a) Mark R. Dickinson, and Amanda J. Wright Department of Physics and Astronomy, University ...
Optics Express, 2006
We report the creation of white-light, achromatic Bessel beams using a spatial light modulator an... more We report the creation of white-light, achromatic Bessel beams using a spatial light modulator and a prism to compensate for the dispersion. Unlike the Bessel beam created by a refractive axicon, this achromatic beam has a radial wavevector and hence an intensity cross-section which is independent of wavelength. The technique also lends itself to the generation of higher order Bessel
Lab on A Chip, 2005
We report the creation of permanent 3D configurations of cells, at predefined positions, within a... more We report the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.
Journal of Environmental Medicine, 2005
We report on the introduction of active optical elements into confocal and multiphoton microscope... more We report on the introduction of active optical elements into confocal and multiphoton microscopes in order to reduce the sample-induced aberration. Using a flexible membrane mirror as the active element, the beam entering the rear of the microscope objective is altered to produce the smallest point spread function once it is brought to a focus inside the sample. The conventional approach to adaptive optics, commonly used in astronomy, is to utilise a wavefront sensor to determine the required mirror shape. We have developed a technique that uses optimisation algorithms to improve the returned signal without the use of a wavefront sensor. We have investigated a number of possible optimisation methods, covering hill climbing, genetic algorithms, and more random search methods. The system has demonstrated a significant enhancement in the axial resolution of a confocal microscope when imaging at depth within a sample. We discuss the trade-offs of the various approaches adopted, comparing speed with resolution enhancement.
Optical Trapping and Optical Micromanipulation VIII, 2011
We present results demonstrating for the first time that an optically trapped bead can be used as... more We present results demonstrating for the first time that an optically trapped bead can be used as a local probe to measure the variation in the viscoelastic properties of the vitreous humor of a rabbit eye. The Brownian motion of the optically trapped bead was monitored on a fast CCD camera on the millisecond timescale. Analysis of the bead trajectory