Amandeep Singh - Academia.edu (original) (raw)
Papers by Amandeep Singh
Journal of Drug Delivery and Therapeutics, 2020
Regardless of so many advancements in the treatment, colon cancer still stands third in cancer-re... more Regardless of so many advancements in the treatment, colon cancer still stands third in cancer-related deaths worldwide. Toxicity associated with conventional drugs is one of the major problems associated with chemotherapy. Targeted delivery works by concentrating the medication in the tissues of interest and reducing the concentration in remaining tissues. This delivery system helps the drug molecule to reach preferably to the desired site. The targeting will lower the requirement of a higher dose of the drug thus reducing the dosage frequency. The present review focuses on the various parameters of targeted drug delivery including the criteria for selection of drug and factors affecting the targeted drug delivery and also includes the brief discussion about different targeted drug deliveries for colon cancer therapies. Keywords: colon cancer, targeted drug delivery, chemotherapies
Annals of Internal Medicine, 2015
Journal of clinical and diagnostic research : JCDR, 2015
Preoperative evaluation in patients with colorectal carcinoma is essential for a correct therapeu... more Preoperative evaluation in patients with colorectal carcinoma is essential for a correct therapeutic plan. Conventional colonoscopy has certain limitations including its inability to detect synchronous lesions in case of distal obstructive mass and inaccurate tumour localization. CT colonography combines cross sectional imaging with virtual colonoscopic images and offers a comprehensive preoperative evaluation in patients with colorectal carcinoma including detection of synchronous lesions with accurate segmental localization and loco regional staging. The objective was to determine the role of CT colonography in various colonic lesions and to correlate the findings with conventional colonoscopy and histopathological findings. This prospective study included 50 patients with clinical symptoms suspicious of colonic pathology. All the patients underwent both CT colonography and conventional colonoscopy on the same day. CT colonography was performed in supine and prone position. Consid...
Letters in Drug Design & Discovery, 2013
ABSTRACT In soybean, a small hormone like peptide, leginsulin was found to bind Basic 7S Globulin... more ABSTRACT In soybean, a small hormone like peptide, leginsulin was found to bind Basic 7S Globulin (Bg7S) and stimulate its tyrosine kinase activity. The NMR-structure of leginsulin, along with crystal structure of Bg7S, was used to create a rigid docking model, followed by flexible refinement of the interaction complex. This study provides structural insights into the binding of leginsulin to Bg7S. The complex is stabilized predominantly by hydrophobic forces and hydrogen bonds. The cleft is lined to a large extent by the cysteine rich region of the α-subunit of chain D as well as the carboxyl-terminal region of α-subunit of chain A. The model is in agreement with the available experimental evidence for the hot-spot regions of Bg7S and leginsulin. We also observed another similar site on the surface of Bg7S by virtue of its pseudo-222 symmetry. We have further hypothesized that two leginsulin molecules may bind to Bg7S in its crystal structure or tetrameric form.
The European Physical Journal A, 2014
Critical Reviews in Food Science and Nutrition, 2013
Abstract Storage proteins of soybean mostly consist of globulins, which are classified according ... more Abstract Storage proteins of soybean mostly consist of globulins, which are classified according to their sedimentation coefficient. Among 4 major types: 2S, 7S, 11S and 15S of globulins, 7S and 11S constitute major fraction. The 11S fraction consists only of glycinin and 7S fraction majorly consists of β-conglycinin, small amounts of γ-conglycinin and basic 7S globulin (Bg7S). Glycinin exist as a hexamer while β-conglycinin as a trimer and Bg7S as a tetramer. Glycinin subunits are coded by 5 genes of a family, whereas about 15 genes are present for β-conglycinin subunits. Bg7S gene is present in 4 copies in soybean genome. Synthesis of all proteins takes place as a single polypeptide chain, which is cleaved after folding to yield different chains or subunits. Glycinin and β-Conglycinin are made for storage purpose however; Bg7S has potential xylanase inhibition activity and protein kinase activity. Primary structure of Bg7S reveals 12 conserved cysteine residues involved in forming 6 disulfide bonds, which provides appreciable stability to protein. Secondary structure is predominately rich in β-sheets with few alpha helices. Bg7S shares structural similarity with various aspartic-proteases. In this review our aim is to discuss sequence, structure and function of various globulins present in Glycine max.
The Journal of Emergency Medicine, 2011
Journal of Drug Delivery and Therapeutics, 2020
Regardless of so many advancements in the treatment, colon cancer still stands third in cancer-re... more Regardless of so many advancements in the treatment, colon cancer still stands third in cancer-related deaths worldwide. Toxicity associated with conventional drugs is one of the major problems associated with chemotherapy. Targeted delivery works by concentrating the medication in the tissues of interest and reducing the concentration in remaining tissues. This delivery system helps the drug molecule to reach preferably to the desired site. The targeting will lower the requirement of a higher dose of the drug thus reducing the dosage frequency. The present review focuses on the various parameters of targeted drug delivery including the criteria for selection of drug and factors affecting the targeted drug delivery and also includes the brief discussion about different targeted drug deliveries for colon cancer therapies. Keywords: colon cancer, targeted drug delivery, chemotherapies
Annals of Internal Medicine, 2015
Journal of clinical and diagnostic research : JCDR, 2015
Preoperative evaluation in patients with colorectal carcinoma is essential for a correct therapeu... more Preoperative evaluation in patients with colorectal carcinoma is essential for a correct therapeutic plan. Conventional colonoscopy has certain limitations including its inability to detect synchronous lesions in case of distal obstructive mass and inaccurate tumour localization. CT colonography combines cross sectional imaging with virtual colonoscopic images and offers a comprehensive preoperative evaluation in patients with colorectal carcinoma including detection of synchronous lesions with accurate segmental localization and loco regional staging. The objective was to determine the role of CT colonography in various colonic lesions and to correlate the findings with conventional colonoscopy and histopathological findings. This prospective study included 50 patients with clinical symptoms suspicious of colonic pathology. All the patients underwent both CT colonography and conventional colonoscopy on the same day. CT colonography was performed in supine and prone position. Consid...
Letters in Drug Design & Discovery, 2013
ABSTRACT In soybean, a small hormone like peptide, leginsulin was found to bind Basic 7S Globulin... more ABSTRACT In soybean, a small hormone like peptide, leginsulin was found to bind Basic 7S Globulin (Bg7S) and stimulate its tyrosine kinase activity. The NMR-structure of leginsulin, along with crystal structure of Bg7S, was used to create a rigid docking model, followed by flexible refinement of the interaction complex. This study provides structural insights into the binding of leginsulin to Bg7S. The complex is stabilized predominantly by hydrophobic forces and hydrogen bonds. The cleft is lined to a large extent by the cysteine rich region of the α-subunit of chain D as well as the carboxyl-terminal region of α-subunit of chain A. The model is in agreement with the available experimental evidence for the hot-spot regions of Bg7S and leginsulin. We also observed another similar site on the surface of Bg7S by virtue of its pseudo-222 symmetry. We have further hypothesized that two leginsulin molecules may bind to Bg7S in its crystal structure or tetrameric form.
The European Physical Journal A, 2014
Critical Reviews in Food Science and Nutrition, 2013
Abstract Storage proteins of soybean mostly consist of globulins, which are classified according ... more Abstract Storage proteins of soybean mostly consist of globulins, which are classified according to their sedimentation coefficient. Among 4 major types: 2S, 7S, 11S and 15S of globulins, 7S and 11S constitute major fraction. The 11S fraction consists only of glycinin and 7S fraction majorly consists of β-conglycinin, small amounts of γ-conglycinin and basic 7S globulin (Bg7S). Glycinin exist as a hexamer while β-conglycinin as a trimer and Bg7S as a tetramer. Glycinin subunits are coded by 5 genes of a family, whereas about 15 genes are present for β-conglycinin subunits. Bg7S gene is present in 4 copies in soybean genome. Synthesis of all proteins takes place as a single polypeptide chain, which is cleaved after folding to yield different chains or subunits. Glycinin and β-Conglycinin are made for storage purpose however; Bg7S has potential xylanase inhibition activity and protein kinase activity. Primary structure of Bg7S reveals 12 conserved cysteine residues involved in forming 6 disulfide bonds, which provides appreciable stability to protein. Secondary structure is predominately rich in β-sheets with few alpha helices. Bg7S shares structural similarity with various aspartic-proteases. In this review our aim is to discuss sequence, structure and function of various globulins present in Glycine max.
The Journal of Emergency Medicine, 2011