Amany Dapgh - Academia.edu (original) (raw)

Papers by Amany Dapgh

Assiut Veterinary Medical Journal, 2009

International Journal of Veterinary Science

Listeria monocytogenes is considered one of the major potential foodborne pathogens which often t... more Listeria monocytogenes is considered one of the major potential foodborne pathogens which often threaten human health and life. Our study assessed both existence and virulence of L. monocytogenes in raw milk and different milk byproducts in Damietta Governorate, Egypt. One hundred and fifty random samples of raw milk, Kariesh cheese, Domiati cheese, Roomi cheese, yogurt and cream (twenty-five samples for each) were gathered from small retails and various supermarkets at for isolation of L. monocytogenes as well as direct detection of the pathogen in the samples’ using PCR technique that targeting 16S rRNA. Also, the study aimed to investigate the presence of virulence genes; (hly A, inl A, inl B, prf A, plc A) in L. monocytogenes isolates. The PCR findings for direct detection of L. monocytogenes declared that 41 out 150 examined samples were positive to 16S rRNA meanwhile by culture methods L. monocytogenes was recovered from 36 out of 150 examined samples. With regard to detection...

International journal of health sciences

Enteropathogenic Escherichia coli (EPEC) were considered prime reason of diarrhea among neonatal ... more Enteropathogenic Escherichia coli (EPEC) were considered prime reason of diarrhea among neonatal livestock animals in developing countries and could be of public health importance via contaminated milk and meat. Continual attempts were conducted to combat this illness using various antigenic determinants. This study was performed on four E. coli serotypes O26, O45, O103 and O111, which were previously recovered from diarrheic camel calves in Giza, Egypt. Extraction of fimbrial proteins was carried out through dialysis then evaluation of their immunogenic activity was preceded. SDS-PAGE electrophoresis was performed on crude extracted fimbrial proteins and revealed single band for each isolate ranged from 22 to 33kDa. Immunobloting was implemented on the extracted crude fimbrial proteins against E.coli O26 antisera formerly prepared in rabbit.These findings suggested that the fimbrial proteins are of immunogenicity importance and can serve as a protective passive vaccine antigen in ...

Egyptian Journal of Chemistry and Environmental Health

Paratuberculosis. in cattle is an infectious disease caused by Mycobacterium avium supspecies par... more Paratuberculosis. in cattle is an infectious disease caused by Mycobacterium avium supspecies paratuberculosis (MAP) .Paratuberculosis or Johne's disease(JD) is a Chronic debilitating disease that affects a wide range of animal hosts. So, this study was designed to focus on diagnosis of paratuberculosis by traditional culture method and ELISA. A total of 165 Egyptian cattle (more than 5 years old) were collected from some private farms in different districts at El Sharkia governorate,. All diseased (155) cattle were in poor condition with marked reduction in milk production, chronic or intermittent diarrhea and showed no fever. From which, fecal , milk and serum samples were collected as well as from other 10 contact apparently normal animals. The Fecal and milk samples were decontaminated and were inoculated onto Herrold's egg yolk medium (HEYM) slants supplemented with mycobactin j., and the slants were incubated at 37°c and observed every 2 weeks for 16 weeks. The suspected growth represented by grayish white rough colonies were smeared and stained by Z.N stain then microscopy examined. The indirect EL1SA was carried out on the serum and milk samples using commercial ELISA kit as well as the use of modified ELISA. The obtained results revealed that out of 165 collected samples from. 13 fecal samples were positive for detection of MAP.by culture method from (155) diarrheic cows and two samples from 10 apparently healthy contact cows with a percentage of 7.9 % and 20 % respectively. The results of indirect ELISA by the using the commercial kit revealed that, 18 serum samples out of 165 (10.9%) tested cow samples were positive and were harboring specific antibodies against MAP (16 cases out of 155 diseased animals and two out of 10 apparently healthy contact cows). Using ELISA with W. antigen detected 19 positive cases out of 165 examined caws and ELISA with S. antigen detected of (21) positive cases. The diagnostic sensitivity and specificity for JD, by the S.ELISA where greater and showed highly detection rate of paratuberculosis infection.

Egyptian Journal of Chemistry and Environmental Health

Mycobacterium Ulcerans infection or Buruli Ulcer is the third most frequent mycobacterial disease... more Mycobacterium Ulcerans infection or Buruli Ulcer is the third most frequent mycobacterial disease in humans .The epidemiology of BU is poorly understood. Some evidence exists for an environmental reservoir associated with slow-flowing or stagnant water. However, culture of M. ulcerans from the environment has never been successful. Treatment of advanced disease is often difficult and complicated by persistence and replace. Surgery is still considered the main treatment option despite its poor acceptability, high costs, and failure to prevent recurrence. In lab diagnosis ,Mycobacterium ulcerans isolates are sensitive to decontamination methods. All decontamination methods currently used for the isolation of M. ulcerans from clinical specimensMycobacterium ulcerans belongs to a group of mycobacteria that are potentially pathogenic for humans and animals. These are sometimes called 'opportunistic mycobacteria' or 'occasional pathogens'. Most species belonging to this group are ubiquitous in nature, and may become pathogenic under special circumstances. These mycobacteria generally cause mycobacterial diseases that are not contagious. The disease in pet animals is considered of great importance in public health issue, some records of the mycobacterium ulcerans in cats were reported and discussed the epidemiological relationship with the buruli ulcer infection in contact women in some countries. The development of PCR for quick identification of M. ulcerans in clinical and environmental samples has greatly improved the diagnostic yield as well as our understanding of the epidemiology of Buruli ulcer.

Assiut Veterinary Medical Journal

Veterinary World

Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease that causes huge economic loss... more Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease that causes huge economic losses. This study aimed to compare the result obtained from the single intradermal test, conventional methods (culture and microscopy), gamma-interferon (IFN-γ) assay, and indirect enzyme-linked immunosorbent assay (ELISA) to diagnose bovine TB. Materials and Methods: This study evaluated 2913 animals from milk farms in Cairo, El-Sharkia, and El-Qalyubia Governorates by single intradermal cervical tuberculin technique (SICTT), ELISA, and IFN-γ assay. Results: Of the 2913 dairy cows surveyed, 3.7% yielded positive results. Culture prepared samples on Lowenstein-Jensen and Middlebrook 7H10 agar media yielded 52 (1.85%) isolates of Mycobacterium spp. from 2805 milk samples that yielded negative tuberculin reactions and 56 (51.85%) isolates of Mycobacterium spp. were recovered from 108 lymph node samples from positive cases. ELISA analysis of the sera of 108 positive SICTT reactors revealed tha...

Alexandria Journal of Veterinary Sciences, 2022

A total of 145clinical and environmental sampleswere collected from(35pig, 30goat, 25sheep,40 rab... more A total of 145clinical and environmental sampleswere collected from(35pig, 30goat, 25sheep,40 rabbit and15drinking water sources) either apparently healthy or showed signs of diarrhea. The samples were cultured onto enriched and specific media (nutrient, blood and MacConkey agar and Aeromonas agar base with ampicillin) and identified biochemically. Aeromonas hydrophila isolated from apparent healthy, diarrheic animals and water sources with incidence of 24.2%, 25.6%, and 66.6% respectively. The impact of certain disinfectants; chlorine, formalin and virkon`S wasfulfilled using the minimum inhibitory concentration (MIC) method. The chlorine disinfectant was checkedat concentrations; 200, 1000, 5000 and 25000part per million in various times:5,10,15,30 minutes for each concentration. It was found that chlorine induced complete destruction at 200 ppm and 1000 ppm after 15 and 30 minutes respectively. Higher concentrations, 5000 ppm and 25000ppm managed to eliminate the microorganism co...

Mycobacterium bovis is a vigorous virulent microorganism that causes bovine tuberculosis a global... more Mycobacterium bovis is a vigorous virulent microorganism that causes bovine tuberculosis a global infectious disease of cattle result in great economic losses. The high public health significance of the organism is attributed to its zoonotic importance as it can infect human population. The rapid and accurate diagnosis of M. bovis is essential to early control and avoiding the enormous bad consequences of the disease. The study was conducted to assess the effectiveness of novel techniques as Gamma interferon assay and polymerase chain reaction compared with ELISA and conventional bacteriological culture in diagnosis of M. bovis.A number of 780 cross-breed dairy cattle in Kafr EL-Shiekh governorate were examined by comparative intradermal tuberculin test during 2018. Milk samples were collected from positive reactors for conduction of bacteriological examinationand polymerase chain reaction.Serumsamples were collected for ELISA using bovine purified protein derivative as well as whol...

January-2021, 2021

Background and Aim: Camels are important livestock in Egypt on cultural and economic bases, but s... more Background and Aim: Camels are important livestock in Egypt on cultural and economic bases, but studies of etiological agents of camelid diseases are limited. The enteropathogen Escherichia coli is a cause of broad spectrum gastrointestinal infections among humans and animals, especially in developing countries. Severe infections can lead to death. The current study aimed to identify pathogenic E. coli strains that cause diarrhea in camel calves and characterize their virulence and drug resistance at a molecular level. Materials and Methods: Seventy fecal samples were collected from diarrheic neonatal camel calves in Giza Governorate during 2018-2019. Samples were cultured on a selective medium for E. coli, and positive colonies were confirmed biochemically, serotyped, and tested for antibiotic susceptibility. E. coli isolates were further confirmed through detection of the housekeeping gene, yaiO, and examined for the presence of virulence genes; traT and fimH and for genes respons...

South Asian Journal of Research in Microbiology, 2020

Aims: one of the most important foodborne microorganisms is the Gram’s positive environmental wid... more Aims: one of the most important foodborne microorganisms is the Gram’s positive environmental wide spread Listeria spp. As the Listeria may be considered a public health concern so there is in needing to rapid, precise and reliable diagnosis of the organism in consumed food. The present study aimed to survey the presence of Listeria spp. among two popular consuming Egyptian white soft cheese using advanced biochemical, antibiotic susceptibility and molecular techniques. Methodology: Listeria spp. was investigated in 155 samples of two white soft cheeses (70 kareish cheese and 85 Damietta cheese) collected from street vendors and retail markets in Giza. The existence of Listeria spp. was tested through cultural and the identification was confirmed biochemically by Vitek2 compact system as well as molecular identification via diplex real time PCR using species specific primers. Results: The results of the study revealed the isolation of two Listeria spp. in a total number of 22 from15...

Zagazig Veterinary Journal, 2013

Scandinavian Journal of Infectious Diseases, 2003

Restriction fragment length polymorphism (RFLP) analysis was used to study the molecular epidemio... more Restriction fragment length polymorphism (RFLP) analysis was used to study the molecular epidemiology of tuberculosis (TB) in certain areas of Tehran. 120 isolates of Mycobacterium tuberculosis, including drug-resistant strains (n = 23), were analysed using polymorphic GC-rich sequence (PGRS) and IS6110 probes. There was considerable diversity among the strains cultured from patients from certain areas. The results of RFLP showed that multidrug resistant (MDR) isolates of M. tuberculosis in Tehran belong to a group of strains with low copies of IS6110 and PGRS. The degree of clustering was higher for the drug-resistant strains than for the susceptible ones (65% vs 20%). Based on the demographic data and results of RFLP, it appears that recent transmissions of TB from old patients have occurred in Tehran. However, drug-resistant TB in the city is mainly caused by strains that look different from those cultured from such patients. The majority of MDR isolates (85%) in this study contained a low copy number of IS6110 and PGRS in RFLP, and were mostly recovered from immigrants and refugees.

Veterinary World, Oct 1, 2019

Background: Pseudomonas aeruginosa is a pit of an enormous group of free-living bacteria that are... more Background: Pseudomonas aeruginosa is a pit of an enormous group of free-living bacteria that are able to live everywhere and suggested to be the causative agent of great scope of acute and chronic animal infections. Aim: The current study was carried out to illustrate the prevalence of P. aeruginosa in small ruminants and existence of some virulence operons as well as its antimicrobial resistance. Materials and Methods: A total of 155 samples from sheep and 105 samples from goats (mouth abscesses, fecal swabs, nasal, tracheal swabs, and lung tissue) were collected for bacteriological study, existence of some virulence expression operons with the study of their sensitivity to the antimicrobials using disc diffusion and presence of mexR operon which is responsible for multidrug resistance (MDR). Results: The bacteriological examination revealed that P. aeruginosa was isolated from nine out of 155 samples from sheep (5.8%) and four isolates out of 105 samples from goat (3.8%). It is found that 12 (92.3%), 10 (76.9 %), and 8 (61.5%) of P. aeruginosa isolates harbored hemolysin phospholipase gene (pclH), gene (exoS), and enterotoxin gene (toxA), respectively. The results of antibiotic sensitivity test showed that all tested isolates were resistant to ampicillin, bacitracin, erythromycin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, and tobramycin but sensitive to ciprofloxacin and norfloxacin. The MDR (mexR) operon was existed in all isolates. Conclusion: There is a growing risk for isolation of virulent MDR P. aeruginosa from sheep and goat illness cases, and this should be regarded in the efficient control programs.

Veterinary World, Oct 14, 2020

Background and Aim: Mycobacterium tuberculosis complex (MTBC) is a group of mycobacteria that are... more Background and Aim: Mycobacterium tuberculosis complex (MTBC) is a group of mycobacteria that are important human pathogens. Mycobacterium tuberculosis and Mycobacterium bovis cause serious chronic life-threatening disease and also significant economic losses in both production and remedication. Recently, emergence of multidrug-resistant tuberculosis (MDR-TB) complex has generated global recognition of the need for rapid and sensitive diagnosis and development of new treatments. The current study illustrates the isolation/identification of MTBC strains in specimens obtained from cows and humans by conventional and real-time polymerase chain reaction (RT-PCR) techniques. Further, the study assesses sensitivity to antituberculosis drugs in isolated MDR strains.

Materials and Methods: A total of 1464 samples from cattle (1285 raw milk and 179 lymph node), and 149 human sputum samples, were collected from farms and abattoirs in Delta Egypt. Conventional methods (culture and Ziehl–Neelsen staining) were implemented as were RT-PCR using MTBC universal DNA. The effect of some antituberculosis drugs on obtained isolates was assayed using drug susceptibility proportion and qualitative suspension techniques.

Results: The MBTC detection rate using the culture method was higher than for Ziehl–Neelsen staining; raw cow milk (2.56 vs. 1.63%), lymph nodes (51.59 vs. 48.04%), and human sputum (5.36 vs. 4.02%). A total of 135 isolates were obtained. Application of RT-PCR detected 138 isolates from the same set of samples. MBTC isolates were resistant to first-line antituberculosis drugs, such as pyrazinamide, isoniazid, rifampicin, and ethambutol by 78.5, 59.3, 40.7, and 31.8%, respectively, and could be highly resistant to kanamycin (82.3%) and amikacin (80.7%). However, isolates remained sensitive to ciprofloxacin (71.1%) and clarithromycin (73.3%) as second-line drugs.

Conclusion: There is a growing risk for isolation of MDR-TB from raw milk and lymph nodes of field tuberculin positive cattle as well as sputum of veterinarians and workers existed in farms and abattoirs. PCR-based techniques have become the gold standard for the identification of mycobacterial species, showing high efficiency compared to bacteriological and microscopic examination. Application of the first- and second-line antituberculosis drugs in combination could counter the MDR-TB concern once infections are identified.

Assiut Veterinary Medical Journal, 2009

International Journal of Veterinary Science

Listeria monocytogenes is considered one of the major potential foodborne pathogens which often t... more Listeria monocytogenes is considered one of the major potential foodborne pathogens which often threaten human health and life. Our study assessed both existence and virulence of L. monocytogenes in raw milk and different milk byproducts in Damietta Governorate, Egypt. One hundred and fifty random samples of raw milk, Kariesh cheese, Domiati cheese, Roomi cheese, yogurt and cream (twenty-five samples for each) were gathered from small retails and various supermarkets at for isolation of L. monocytogenes as well as direct detection of the pathogen in the samples’ using PCR technique that targeting 16S rRNA. Also, the study aimed to investigate the presence of virulence genes; (hly A, inl A, inl B, prf A, plc A) in L. monocytogenes isolates. The PCR findings for direct detection of L. monocytogenes declared that 41 out 150 examined samples were positive to 16S rRNA meanwhile by culture methods L. monocytogenes was recovered from 36 out of 150 examined samples. With regard to detection...

International journal of health sciences

Enteropathogenic Escherichia coli (EPEC) were considered prime reason of diarrhea among neonatal ... more Enteropathogenic Escherichia coli (EPEC) were considered prime reason of diarrhea among neonatal livestock animals in developing countries and could be of public health importance via contaminated milk and meat. Continual attempts were conducted to combat this illness using various antigenic determinants. This study was performed on four E. coli serotypes O26, O45, O103 and O111, which were previously recovered from diarrheic camel calves in Giza, Egypt. Extraction of fimbrial proteins was carried out through dialysis then evaluation of their immunogenic activity was preceded. SDS-PAGE electrophoresis was performed on crude extracted fimbrial proteins and revealed single band for each isolate ranged from 22 to 33kDa. Immunobloting was implemented on the extracted crude fimbrial proteins against E.coli O26 antisera formerly prepared in rabbit.These findings suggested that the fimbrial proteins are of immunogenicity importance and can serve as a protective passive vaccine antigen in ...

Egyptian Journal of Chemistry and Environmental Health

Paratuberculosis. in cattle is an infectious disease caused by Mycobacterium avium supspecies par... more Paratuberculosis. in cattle is an infectious disease caused by Mycobacterium avium supspecies paratuberculosis (MAP) .Paratuberculosis or Johne's disease(JD) is a Chronic debilitating disease that affects a wide range of animal hosts. So, this study was designed to focus on diagnosis of paratuberculosis by traditional culture method and ELISA. A total of 165 Egyptian cattle (more than 5 years old) were collected from some private farms in different districts at El Sharkia governorate,. All diseased (155) cattle were in poor condition with marked reduction in milk production, chronic or intermittent diarrhea and showed no fever. From which, fecal , milk and serum samples were collected as well as from other 10 contact apparently normal animals. The Fecal and milk samples were decontaminated and were inoculated onto Herrold's egg yolk medium (HEYM) slants supplemented with mycobactin j., and the slants were incubated at 37°c and observed every 2 weeks for 16 weeks. The suspected growth represented by grayish white rough colonies were smeared and stained by Z.N stain then microscopy examined. The indirect EL1SA was carried out on the serum and milk samples using commercial ELISA kit as well as the use of modified ELISA. The obtained results revealed that out of 165 collected samples from. 13 fecal samples were positive for detection of MAP.by culture method from (155) diarrheic cows and two samples from 10 apparently healthy contact cows with a percentage of 7.9 % and 20 % respectively. The results of indirect ELISA by the using the commercial kit revealed that, 18 serum samples out of 165 (10.9%) tested cow samples were positive and were harboring specific antibodies against MAP (16 cases out of 155 diseased animals and two out of 10 apparently healthy contact cows). Using ELISA with W. antigen detected 19 positive cases out of 165 examined caws and ELISA with S. antigen detected of (21) positive cases. The diagnostic sensitivity and specificity for JD, by the S.ELISA where greater and showed highly detection rate of paratuberculosis infection.

Egyptian Journal of Chemistry and Environmental Health

Mycobacterium Ulcerans infection or Buruli Ulcer is the third most frequent mycobacterial disease... more Mycobacterium Ulcerans infection or Buruli Ulcer is the third most frequent mycobacterial disease in humans .The epidemiology of BU is poorly understood. Some evidence exists for an environmental reservoir associated with slow-flowing or stagnant water. However, culture of M. ulcerans from the environment has never been successful. Treatment of advanced disease is often difficult and complicated by persistence and replace. Surgery is still considered the main treatment option despite its poor acceptability, high costs, and failure to prevent recurrence. In lab diagnosis ,Mycobacterium ulcerans isolates are sensitive to decontamination methods. All decontamination methods currently used for the isolation of M. ulcerans from clinical specimensMycobacterium ulcerans belongs to a group of mycobacteria that are potentially pathogenic for humans and animals. These are sometimes called 'opportunistic mycobacteria' or 'occasional pathogens'. Most species belonging to this group are ubiquitous in nature, and may become pathogenic under special circumstances. These mycobacteria generally cause mycobacterial diseases that are not contagious. The disease in pet animals is considered of great importance in public health issue, some records of the mycobacterium ulcerans in cats were reported and discussed the epidemiological relationship with the buruli ulcer infection in contact women in some countries. The development of PCR for quick identification of M. ulcerans in clinical and environmental samples has greatly improved the diagnostic yield as well as our understanding of the epidemiology of Buruli ulcer.

Assiut Veterinary Medical Journal

Veterinary World

Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease that causes huge economic loss... more Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease that causes huge economic losses. This study aimed to compare the result obtained from the single intradermal test, conventional methods (culture and microscopy), gamma-interferon (IFN-γ) assay, and indirect enzyme-linked immunosorbent assay (ELISA) to diagnose bovine TB. Materials and Methods: This study evaluated 2913 animals from milk farms in Cairo, El-Sharkia, and El-Qalyubia Governorates by single intradermal cervical tuberculin technique (SICTT), ELISA, and IFN-γ assay. Results: Of the 2913 dairy cows surveyed, 3.7% yielded positive results. Culture prepared samples on Lowenstein-Jensen and Middlebrook 7H10 agar media yielded 52 (1.85%) isolates of Mycobacterium spp. from 2805 milk samples that yielded negative tuberculin reactions and 56 (51.85%) isolates of Mycobacterium spp. were recovered from 108 lymph node samples from positive cases. ELISA analysis of the sera of 108 positive SICTT reactors revealed tha...

Alexandria Journal of Veterinary Sciences, 2022

A total of 145clinical and environmental sampleswere collected from(35pig, 30goat, 25sheep,40 rab... more A total of 145clinical and environmental sampleswere collected from(35pig, 30goat, 25sheep,40 rabbit and15drinking water sources) either apparently healthy or showed signs of diarrhea. The samples were cultured onto enriched and specific media (nutrient, blood and MacConkey agar and Aeromonas agar base with ampicillin) and identified biochemically. Aeromonas hydrophila isolated from apparent healthy, diarrheic animals and water sources with incidence of 24.2%, 25.6%, and 66.6% respectively. The impact of certain disinfectants; chlorine, formalin and virkon`S wasfulfilled using the minimum inhibitory concentration (MIC) method. The chlorine disinfectant was checkedat concentrations; 200, 1000, 5000 and 25000part per million in various times:5,10,15,30 minutes for each concentration. It was found that chlorine induced complete destruction at 200 ppm and 1000 ppm after 15 and 30 minutes respectively. Higher concentrations, 5000 ppm and 25000ppm managed to eliminate the microorganism co...

Mycobacterium bovis is a vigorous virulent microorganism that causes bovine tuberculosis a global... more Mycobacterium bovis is a vigorous virulent microorganism that causes bovine tuberculosis a global infectious disease of cattle result in great economic losses. The high public health significance of the organism is attributed to its zoonotic importance as it can infect human population. The rapid and accurate diagnosis of M. bovis is essential to early control and avoiding the enormous bad consequences of the disease. The study was conducted to assess the effectiveness of novel techniques as Gamma interferon assay and polymerase chain reaction compared with ELISA and conventional bacteriological culture in diagnosis of M. bovis.A number of 780 cross-breed dairy cattle in Kafr EL-Shiekh governorate were examined by comparative intradermal tuberculin test during 2018. Milk samples were collected from positive reactors for conduction of bacteriological examinationand polymerase chain reaction.Serumsamples were collected for ELISA using bovine purified protein derivative as well as whol...

January-2021, 2021

Background and Aim: Camels are important livestock in Egypt on cultural and economic bases, but s... more Background and Aim: Camels are important livestock in Egypt on cultural and economic bases, but studies of etiological agents of camelid diseases are limited. The enteropathogen Escherichia coli is a cause of broad spectrum gastrointestinal infections among humans and animals, especially in developing countries. Severe infections can lead to death. The current study aimed to identify pathogenic E. coli strains that cause diarrhea in camel calves and characterize their virulence and drug resistance at a molecular level. Materials and Methods: Seventy fecal samples were collected from diarrheic neonatal camel calves in Giza Governorate during 2018-2019. Samples were cultured on a selective medium for E. coli, and positive colonies were confirmed biochemically, serotyped, and tested for antibiotic susceptibility. E. coli isolates were further confirmed through detection of the housekeeping gene, yaiO, and examined for the presence of virulence genes; traT and fimH and for genes respons...

South Asian Journal of Research in Microbiology, 2020

Aims: one of the most important foodborne microorganisms is the Gram’s positive environmental wid... more Aims: one of the most important foodborne microorganisms is the Gram’s positive environmental wide spread Listeria spp. As the Listeria may be considered a public health concern so there is in needing to rapid, precise and reliable diagnosis of the organism in consumed food. The present study aimed to survey the presence of Listeria spp. among two popular consuming Egyptian white soft cheese using advanced biochemical, antibiotic susceptibility and molecular techniques. Methodology: Listeria spp. was investigated in 155 samples of two white soft cheeses (70 kareish cheese and 85 Damietta cheese) collected from street vendors and retail markets in Giza. The existence of Listeria spp. was tested through cultural and the identification was confirmed biochemically by Vitek2 compact system as well as molecular identification via diplex real time PCR using species specific primers. Results: The results of the study revealed the isolation of two Listeria spp. in a total number of 22 from15...

Zagazig Veterinary Journal, 2013

Scandinavian Journal of Infectious Diseases, 2003

Restriction fragment length polymorphism (RFLP) analysis was used to study the molecular epidemio... more Restriction fragment length polymorphism (RFLP) analysis was used to study the molecular epidemiology of tuberculosis (TB) in certain areas of Tehran. 120 isolates of Mycobacterium tuberculosis, including drug-resistant strains (n = 23), were analysed using polymorphic GC-rich sequence (PGRS) and IS6110 probes. There was considerable diversity among the strains cultured from patients from certain areas. The results of RFLP showed that multidrug resistant (MDR) isolates of M. tuberculosis in Tehran belong to a group of strains with low copies of IS6110 and PGRS. The degree of clustering was higher for the drug-resistant strains than for the susceptible ones (65% vs 20%). Based on the demographic data and results of RFLP, it appears that recent transmissions of TB from old patients have occurred in Tehran. However, drug-resistant TB in the city is mainly caused by strains that look different from those cultured from such patients. The majority of MDR isolates (85%) in this study contained a low copy number of IS6110 and PGRS in RFLP, and were mostly recovered from immigrants and refugees.

Veterinary World, Oct 1, 2019

Background: Pseudomonas aeruginosa is a pit of an enormous group of free-living bacteria that are... more Background: Pseudomonas aeruginosa is a pit of an enormous group of free-living bacteria that are able to live everywhere and suggested to be the causative agent of great scope of acute and chronic animal infections. Aim: The current study was carried out to illustrate the prevalence of P. aeruginosa in small ruminants and existence of some virulence operons as well as its antimicrobial resistance. Materials and Methods: A total of 155 samples from sheep and 105 samples from goats (mouth abscesses, fecal swabs, nasal, tracheal swabs, and lung tissue) were collected for bacteriological study, existence of some virulence expression operons with the study of their sensitivity to the antimicrobials using disc diffusion and presence of mexR operon which is responsible for multidrug resistance (MDR). Results: The bacteriological examination revealed that P. aeruginosa was isolated from nine out of 155 samples from sheep (5.8%) and four isolates out of 105 samples from goat (3.8%). It is found that 12 (92.3%), 10 (76.9 %), and 8 (61.5%) of P. aeruginosa isolates harbored hemolysin phospholipase gene (pclH), gene (exoS), and enterotoxin gene (toxA), respectively. The results of antibiotic sensitivity test showed that all tested isolates were resistant to ampicillin, bacitracin, erythromycin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, and tobramycin but sensitive to ciprofloxacin and norfloxacin. The MDR (mexR) operon was existed in all isolates. Conclusion: There is a growing risk for isolation of virulent MDR P. aeruginosa from sheep and goat illness cases, and this should be regarded in the efficient control programs.

Veterinary World, Oct 14, 2020

Background and Aim: Mycobacterium tuberculosis complex (MTBC) is a group of mycobacteria that are... more Background and Aim: Mycobacterium tuberculosis complex (MTBC) is a group of mycobacteria that are important human pathogens. Mycobacterium tuberculosis and Mycobacterium bovis cause serious chronic life-threatening disease and also significant economic losses in both production and remedication. Recently, emergence of multidrug-resistant tuberculosis (MDR-TB) complex has generated global recognition of the need for rapid and sensitive diagnosis and development of new treatments. The current study illustrates the isolation/identification of MTBC strains in specimens obtained from cows and humans by conventional and real-time polymerase chain reaction (RT-PCR) techniques. Further, the study assesses sensitivity to antituberculosis drugs in isolated MDR strains.

Materials and Methods: A total of 1464 samples from cattle (1285 raw milk and 179 lymph node), and 149 human sputum samples, were collected from farms and abattoirs in Delta Egypt. Conventional methods (culture and Ziehl–Neelsen staining) were implemented as were RT-PCR using MTBC universal DNA. The effect of some antituberculosis drugs on obtained isolates was assayed using drug susceptibility proportion and qualitative suspension techniques.

Results: The MBTC detection rate using the culture method was higher than for Ziehl–Neelsen staining; raw cow milk (2.56 vs. 1.63%), lymph nodes (51.59 vs. 48.04%), and human sputum (5.36 vs. 4.02%). A total of 135 isolates were obtained. Application of RT-PCR detected 138 isolates from the same set of samples. MBTC isolates were resistant to first-line antituberculosis drugs, such as pyrazinamide, isoniazid, rifampicin, and ethambutol by 78.5, 59.3, 40.7, and 31.8%, respectively, and could be highly resistant to kanamycin (82.3%) and amikacin (80.7%). However, isolates remained sensitive to ciprofloxacin (71.1%) and clarithromycin (73.3%) as second-line drugs.

Conclusion: There is a growing risk for isolation of MDR-TB from raw milk and lymph nodes of field tuberculin positive cattle as well as sputum of veterinarians and workers existed in farms and abattoirs. PCR-based techniques have become the gold standard for the identification of mycobacterial species, showing high efficiency compared to bacteriological and microscopic examination. Application of the first- and second-line antituberculosis drugs in combination could counter the MDR-TB concern once infections are identified.