Amany Ramah - Independent Researcher (original) (raw)

Papers by Amany Ramah

Research paper thumbnail of Effects of Maternal Supplementation with Organic Trace Minerals including Zinc, Manganese, Copper, and Cobalt during the Late and Post-Partum Periods on the Health and Immune Status of Japanese Black Calves

MDPI, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Animals 14 00346 (1)

MDPI, 2024

Maternal colostrum (MC) is an important source of nutrients and immune factors for newborn calves... more Maternal colostrum (MC) is an important source of nutrients and immune factors for
newborn calves. However, when colostrum is unavailable or of poor quality, a colostrum replacer
(CR) may be a suitable alternative to MC. As stock-raising farmers must make informed decisions
about colostrum feeding management, this study was conducted to determine the effect of feeding
MC versus CR on the promotion of immunological status, growth, and health in pre-weaned Japanese
black (JB) calves. Sixteen newborn JB calves were fed MC after birth, and 16 JB calves were fed CR. For
the MC group, the numbers of γδ T cells, CD4+
cells, CD8+
cells, CD4+CD8+
cells, B cells, and MHC
class II+
cells were significantly higher compared with the CR group. Furthermore, the expression
levels of interleukin (IL)-1β-, IL-2-, and interferon-γ (IFN-γ)-encoding mRNAs were significantly
higher in the MC group compared with the CR group. A lower incidence of disease in 1-month-old
calves and higher carcass weight in the MC group were observed compared with the CR group.
These results suggest that CR activates the immune system delayed in calves compared with MC.
MC increases populations of various immunocompetent cells, which can reduce infection rates and
improve body weight gain

Research paper thumbnail of Anatomical View of the Internal Carotid Artery Occlusion in Japanese Black Cattle

MDPI, 2024

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology

The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different phys... more The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different physiological responses of male African catfish, Clarias gariepinus, and the ameliorative effect of Chlorella vulgaris. The fish (160 ± 5.10 g) were exposed to 1/20 LC 50 of OXY (0.58 mg/L) for 60 consecutive days with or without coadministration of C. vulgaris (25 g/kg diet) in triplicate groups. The results revealed that chronic exposure to a sublethal level of OXY induced severe anemia and leukopenia. OXY-exposed fish experienced hypoproteinemia, marked lower AchE levels, and a significant increase in glucose, liver, and kidney function biomarkers. The DNA fragmentation of the liver increased by 15 % in fish compared to the control. On the other hand, lipid peroxidation, superoxide dismutase, and catalase activities were markedly increased in the liver and testes homogenates of the OXY-exposed fish. Meanwhile, total antioxidant capacity and glutathione S-transferase levels declined in the same tissues. Exposure to OXY induced a significant reduction in testosterone and luteinizing hormone levels and a significant increase in follicle stimulating hormone and estradiol. Meanwhile, C. vulgaris dietary supplementation succeeded in alleviating the negative impact of OXY on hematobiochemical parameters and restoring the antioxidant balance in the liver and testes. Furthermore, it ameliorated endocrine disruption and repaired sex hormone levels. In conclusion, exposure to OXY could induce systemic stress, oxidative stress, and endocrine disruption in male C. gariepinus. The dietary supplementation of C. vulgaris could be a potential protective strategy against the toxicity of OXY.

Research paper thumbnail of Protective effects of probiotics against tannin-induced immunosuppression in broiler chickens

BMFH, 2022

Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for live... more Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for livestock. However, our previous study described the dose-dependent adverse effects of TA on immune responses and growth in chickens. In this study, we evaluated the protective effects of a probiotic preparation (BT) consisting of three different bacteria (Bacillus mesenteric, Clostridium butyricum, and Streptococcus faecalis) against TA-induced immunosuppression in chickens. Forty chicks were divided into 4 groups as follows: the CON group (basal diet), BT group supplemented with 3 g BT/kg diet, tannic acid (TA) group supplemented with 30 g TA/kg diet, and BT+TA group supplemented with 3 g BT/kg diet + 30 g TA/kg diet. The feeding trial lasted for 35 days. Lymphocyte subset, macrophage phagocytosis, cytokine mRNA expression, and primary and secondary IgY immune responses were evaluated. BT supplementation significantly improved TA-induced reductions in final body weight, body weight gain, feed intake, and relative weights of lymphoid organs compared with the TA group. Furthermore, in the spleen and cecal tonsil (CT), the relative populations of CD4 + , CD8 + , and CD4 + CD8 + cells in the BT+TA group were significantly ameliorated compared with the TA group. Additionally, comparison with the TA group showed that the chickens in the BT+TA group had an improved relative population of B cells in the CT and that macrophage phagocytosis in the spleen was significantly increased. Chickens in the BT+TA group showed significant increases in IFN-γ and IL-4 mRNA expression in the spleen compared with the TA group. The primary and secondary IgY responses were significantly improved. These results revealed that supplementation with BT protects against TA-induced immunosuppression in chickens.

Research paper thumbnail of Protective effects of probiotics against tannin-induced immunosuppression in broiler chickens

Bioscience of Microbiota, Food and Health

Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for live... more Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for livestock. However, our previous study described the dose-dependent adverse effects of TA on immune responses and growth in chickens. In this study, we evaluated the protective effects of a probiotic preparation (BT) consisting of three different bacteria (Bacillus mesenteric, Clostridium butyricum, and Streptococcus faecalis) against TA-induced immunosuppression in chickens. Forty chicks were divided into 4 groups as follows: the CON group (basal diet), BT group supplemented with 3 g BT/kg diet, tannic acid (TA) group supplemented with 30 g TA/kg diet, and BT+TA group supplemented with 3 g BT/kg diet + 30 g TA/kg diet. The feeding trial lasted for 35 days. Lymphocyte subset, macrophage phagocytosis, cytokine mRNA expression, and primary and secondary IgY immune responses were evaluated. BT supplementation significantly improved TA-induced reductions in final body weight, body weight gain, feed intake, and relative weights of lymphoid organs compared with the TA group. Furthermore, in the spleen and cecal tonsil (CT), the relative populations of CD4 + , CD8 + , and CD4 + CD8 + cells in the BT+TA group were significantly ameliorated compared with the TA group. Additionally, comparison with the TA group showed that the chickens in the BT+TA group had an improved relative population of B cells in the CT and that macrophage phagocytosis in the spleen was significantly increased. Chickens in the BT+TA group showed significant increases in IFN-γ and IL-4 mRNA expression in the spleen compared with the TA group. The primary and secondary IgY responses were significantly improved. These results revealed that supplementation with BT protects against TA-induced immunosuppression in chickens.

Research paper thumbnail of Studies on the immune status of calves with chronic inflammation and thymus atrophy

Journal of Veterinary Medical Science

The thymus is a primary lymphoid organ where the primary T cell repertoire is generated. Thymus a... more The thymus is a primary lymphoid organ where the primary T cell repertoire is generated. Thymus atrophy is induced by various conditions, including infectious diseases, glucocorticoid treatment, and poor breeding management. Cattle with thymus atrophy tend to exhibit weak calf syndrome, a condition in which approximately half of neonates die shortly after birth. Calves with thymus atrophy that survive the first month typically contract chronic inflammatory diseases. In this study, we analyzed the populations of the peripheral blood mononuclear cells and thymocytes in calves with thymus atrophy. In addition, we evaluated polarization of master gene and cytokine mRNA expression in peripheral blood CD4 + cells in the calves. The population of CD4 + CD8 + cells in thymus of the calves with thymus atrophy was lower than that of control calves. IL10 mRNA expression in peripheral blood CD4 + cells of calves with thymus atrophy was significantly lower than that of control calves. TBX21 mRNA expression in peripheral CD4 + cells of thymus atrophy calves was tended to be higher than that of the control group. In addition, FOXP3 mRNA expression in peripheral CD4 + cells of the thymus atrophy calves was tended to be lower than that of the control calves. Thymus atrophy calves exhibited chronic inflammatory disease leading, in severe situations, to conditions such as pneumonia with caseous necrosis. These severe inflammatory responses likely are due to decreases in IL10 mRNA expression, impairing control of macrophages, one of the main cell fractions of natural immunity.

Research paper thumbnail of Effects of branched-chain amino acids on immune status of young racing horses

Journal of Veterinary Medical Science, 2022

High-intensity exercise and competition are associated with depressed immune function. Young hors... more High-intensity exercise and competition are associated with depressed immune function. Young horses, which participate in high-intensity exercise and competitions, are at increased risk for the development of infectious disease due to depression of immune function. The effects of branched-chain amino acid (BCAA) supplementation on the immune status of young racing horses were evaluated, determining whether BCAA might help to avoid or reduce immune suppression during exercise and competitions. Twenty horses (10 male and 10 female) were treated with BCAA supplementation; another twenty untreated horses (10 male and 10 female) constituted control group. Peripheral blood was collected from each animal and evaluated for lymphocyte subsets, phagocytosis analysis of monocytes and granulocytes, lymphocyte proliferative response, and expression of cytokine-encoding messenger ribonucleic acids (mRNAs). The numbers of CD4 + , CD8 + , and major histocompatibility complex (MHC) class II + cells in females of the treated group were significantly higher than those in females of the control group. The lymphocyte proliferative response in female of the treated group also was significantly higher than that in females of the control group. In addition, expression of mRNAs encoding interleukin-1β (IL-1β) and interferon-γ (IFN-γ) in females of the treated group was significantly higher than that in females of the control group. There were no significant differences between males of the treated and control groups. The results of this study indicated the positive effects of BCAA supplementation in counteracting immunosuppression in young female racing horses during and following high-intensity exercise.

Research paper thumbnail of Different doses of tannin reflect a double-edged impact on broiler chicken immunity

Veterinary Immunology and Immunopathology, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of The effect of lead toxicity on male albino rats reproduction with ameliorate by vitamin E and pumpkin seeds oil

Benha Veterinary Medical Journal, 2015

Lead is an industrial pollutant that may exert specific toxic effect on mammals. The aim of study... more Lead is an industrial pollutant that may exert specific toxic effect on mammals. The aim of study was to investigate the protective effect of vit. E and pumpkin seeds oil on male reproductive system of albino rat induced by lead toxicity , the studying toxic effect of lead on body weight , testis weight, Glutathione, Glutathione-s-transferase, lipid peroxide, testosterone, lead residue , sperm gram, Nrf2 gene expression and histopathological change in testes of rat. Sixty male albino rats randomly divided into 4 groups. Group I was given olive oil 3 times a week orally, group II was given 1.5 g/L lead acetate daily in drinking water, group III was given lead acetate plus 600 mg/kg/ bwt vit. E orally 3 times a week, while group IV was given lead acetate plus 288 mg/kg/bwt pumpkin seeds oil orally 3 time a week. The experiment was extended for 8 weeks. Our results revealed significant decrease in body weight, GSH, GST, testosterone hormone level and sperm viability, and significant increase in MDA, concentration of lead, sperm abnormality and expression of Nrf2 gene in group II without any significant changes in testis and epididymis weight. Moreover, the pathological changes in testes showed focal degeneration with loss of spermatogenic series in the seminiferous tubules. All above mentioned result were significantly improved in group III & IV. In conclusion, vitamin E and pumpkin seeds oil have a protective effect on the testicular damage induced by lead.

Research paper thumbnail of Ameliorative Effect of Quercetin against Abamectin-Induced Hemato-Biochemical Alterations and Hepatorenal Oxidative Damage in Nile Tilapia, Oreochromis niloticus

Animals

Abamectin (ABM) is a common agricultural pesticide and veterinary anthelmintic drug. It can disch... more Abamectin (ABM) is a common agricultural pesticide and veterinary anthelmintic drug. It can discharge from the sites of application to aquatic systems via surface run-off or spray drift, causing harmful effects to aquatic organisms. The present study investigated the protective effect of dietary quercetin supplementation on hemato-biochemical parameters and hepato-renal oxidative stress biomarkers in Nile tilapia (Oreochromis niloticus) exposed to a sublethal dose of ABM. Fish were allocated into six equal groups. The first group was kept as a control group. The second and third groups (Q400, and Q800) were fed diets supplemented with two quercetin levels (400 and 800 mg/kg diet), respectively. The fourth group (ABM) was intoxicated with 20.73 µg/L of ABM. The fifth and sixth groups (ABM + Q400, and ABM + Q800) were fed diet supplemented with two quercetin levels (400 and 800 mg/kg diet) and simultaneously intoxicated with ABM for 60 days. The results showed that ABM significantly d...

Research paper thumbnail of Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

Comparative Biochemistry and Physiology, Part C , 2023

The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different phys... more The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different physiological responses of male African catfish, Clarias gariepinus, and the ameliorative effect of Chlorella vulgaris. The fish (160 ± 5.10 g) were exposed to 1/20 LC 50 of OXY (0.58 mg/L) for 60 consecutive days with or without coadministration of C. vulgaris (25 g/kg diet) in triplicate groups. The results revealed that chronic exposure to a sublethal level of OXY induced severe anemia and leukopenia. OXY-exposed fish experienced hypoproteinemia, marked lower AchE levels, and a significant increase in glucose, liver, and kidney function biomarkers. The DNA fragmentation of the liver increased by 15 % in fish compared to the control. On the other hand, lipid peroxidation, superoxide dismutase, and catalase activities were markedly increased in the liver and testes homogenates of the OXY-exposed fish. Meanwhile, total antioxidant capacity and glutathione S-transferase levels declined in the same tissues. Exposure to OXY induced a significant reduction in testosterone and luteinizing hormone levels and a significant increase in follicle stimulating hormone and estradiol. Meanwhile, C. vulgaris dietary supplementation succeeded in alleviating the negative impact of OXY on hematobiochemical parameters and restoring the antioxidant balance in the liver and testes. Furthermore, it ameliorated endocrine disruption and repaired sex hormone levels. In conclusion, exposure to OXY could induce systemic stress, oxidative stress, and endocrine disruption in male C. gariepinus. The dietary supplementation of C. vulgaris could be a potential protective strategy against the toxicity of OXY.

Research paper thumbnail of Effects of Maternal Supplementation with Organic Trace Minerals including Zinc, Manganese, Copper, and Cobalt during the Late and Post-Partum Periods on the Health and Immune Status of Japanese Black Calves

MDPI, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Animals 14 00346 (1)

MDPI, 2024

Maternal colostrum (MC) is an important source of nutrients and immune factors for newborn calves... more Maternal colostrum (MC) is an important source of nutrients and immune factors for
newborn calves. However, when colostrum is unavailable or of poor quality, a colostrum replacer
(CR) may be a suitable alternative to MC. As stock-raising farmers must make informed decisions
about colostrum feeding management, this study was conducted to determine the effect of feeding
MC versus CR on the promotion of immunological status, growth, and health in pre-weaned Japanese
black (JB) calves. Sixteen newborn JB calves were fed MC after birth, and 16 JB calves were fed CR. For
the MC group, the numbers of γδ T cells, CD4+
cells, CD8+
cells, CD4+CD8+
cells, B cells, and MHC
class II+
cells were significantly higher compared with the CR group. Furthermore, the expression
levels of interleukin (IL)-1β-, IL-2-, and interferon-γ (IFN-γ)-encoding mRNAs were significantly
higher in the MC group compared with the CR group. A lower incidence of disease in 1-month-old
calves and higher carcass weight in the MC group were observed compared with the CR group.
These results suggest that CR activates the immune system delayed in calves compared with MC.
MC increases populations of various immunocompetent cells, which can reduce infection rates and
improve body weight gain

Research paper thumbnail of Anatomical View of the Internal Carotid Artery Occlusion in Japanese Black Cattle

MDPI, 2024

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology

The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different phys... more The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different physiological responses of male African catfish, Clarias gariepinus, and the ameliorative effect of Chlorella vulgaris. The fish (160 ± 5.10 g) were exposed to 1/20 LC 50 of OXY (0.58 mg/L) for 60 consecutive days with or without coadministration of C. vulgaris (25 g/kg diet) in triplicate groups. The results revealed that chronic exposure to a sublethal level of OXY induced severe anemia and leukopenia. OXY-exposed fish experienced hypoproteinemia, marked lower AchE levels, and a significant increase in glucose, liver, and kidney function biomarkers. The DNA fragmentation of the liver increased by 15 % in fish compared to the control. On the other hand, lipid peroxidation, superoxide dismutase, and catalase activities were markedly increased in the liver and testes homogenates of the OXY-exposed fish. Meanwhile, total antioxidant capacity and glutathione S-transferase levels declined in the same tissues. Exposure to OXY induced a significant reduction in testosterone and luteinizing hormone levels and a significant increase in follicle stimulating hormone and estradiol. Meanwhile, C. vulgaris dietary supplementation succeeded in alleviating the negative impact of OXY on hematobiochemical parameters and restoring the antioxidant balance in the liver and testes. Furthermore, it ameliorated endocrine disruption and repaired sex hormone levels. In conclusion, exposure to OXY could induce systemic stress, oxidative stress, and endocrine disruption in male C. gariepinus. The dietary supplementation of C. vulgaris could be a potential protective strategy against the toxicity of OXY.

Research paper thumbnail of Protective effects of probiotics against tannin-induced immunosuppression in broiler chickens

BMFH, 2022

Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for live... more Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for livestock. However, our previous study described the dose-dependent adverse effects of TA on immune responses and growth in chickens. In this study, we evaluated the protective effects of a probiotic preparation (BT) consisting of three different bacteria (Bacillus mesenteric, Clostridium butyricum, and Streptococcus faecalis) against TA-induced immunosuppression in chickens. Forty chicks were divided into 4 groups as follows: the CON group (basal diet), BT group supplemented with 3 g BT/kg diet, tannic acid (TA) group supplemented with 30 g TA/kg diet, and BT+TA group supplemented with 3 g BT/kg diet + 30 g TA/kg diet. The feeding trial lasted for 35 days. Lymphocyte subset, macrophage phagocytosis, cytokine mRNA expression, and primary and secondary IgY immune responses were evaluated. BT supplementation significantly improved TA-induced reductions in final body weight, body weight gain, feed intake, and relative weights of lymphoid organs compared with the TA group. Furthermore, in the spleen and cecal tonsil (CT), the relative populations of CD4 + , CD8 + , and CD4 + CD8 + cells in the BT+TA group were significantly ameliorated compared with the TA group. Additionally, comparison with the TA group showed that the chickens in the BT+TA group had an improved relative population of B cells in the CT and that macrophage phagocytosis in the spleen was significantly increased. Chickens in the BT+TA group showed significant increases in IFN-γ and IL-4 mRNA expression in the spleen compared with the TA group. The primary and secondary IgY responses were significantly improved. These results revealed that supplementation with BT protects against TA-induced immunosuppression in chickens.

Research paper thumbnail of Protective effects of probiotics against tannin-induced immunosuppression in broiler chickens

Bioscience of Microbiota, Food and Health

Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for live... more Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for livestock. However, our previous study described the dose-dependent adverse effects of TA on immune responses and growth in chickens. In this study, we evaluated the protective effects of a probiotic preparation (BT) consisting of three different bacteria (Bacillus mesenteric, Clostridium butyricum, and Streptococcus faecalis) against TA-induced immunosuppression in chickens. Forty chicks were divided into 4 groups as follows: the CON group (basal diet), BT group supplemented with 3 g BT/kg diet, tannic acid (TA) group supplemented with 30 g TA/kg diet, and BT+TA group supplemented with 3 g BT/kg diet + 30 g TA/kg diet. The feeding trial lasted for 35 days. Lymphocyte subset, macrophage phagocytosis, cytokine mRNA expression, and primary and secondary IgY immune responses were evaluated. BT supplementation significantly improved TA-induced reductions in final body weight, body weight gain, feed intake, and relative weights of lymphoid organs compared with the TA group. Furthermore, in the spleen and cecal tonsil (CT), the relative populations of CD4 + , CD8 + , and CD4 + CD8 + cells in the BT+TA group were significantly ameliorated compared with the TA group. Additionally, comparison with the TA group showed that the chickens in the BT+TA group had an improved relative population of B cells in the CT and that macrophage phagocytosis in the spleen was significantly increased. Chickens in the BT+TA group showed significant increases in IFN-γ and IL-4 mRNA expression in the spleen compared with the TA group. The primary and secondary IgY responses were significantly improved. These results revealed that supplementation with BT protects against TA-induced immunosuppression in chickens.

Research paper thumbnail of Studies on the immune status of calves with chronic inflammation and thymus atrophy

Journal of Veterinary Medical Science

The thymus is a primary lymphoid organ where the primary T cell repertoire is generated. Thymus a... more The thymus is a primary lymphoid organ where the primary T cell repertoire is generated. Thymus atrophy is induced by various conditions, including infectious diseases, glucocorticoid treatment, and poor breeding management. Cattle with thymus atrophy tend to exhibit weak calf syndrome, a condition in which approximately half of neonates die shortly after birth. Calves with thymus atrophy that survive the first month typically contract chronic inflammatory diseases. In this study, we analyzed the populations of the peripheral blood mononuclear cells and thymocytes in calves with thymus atrophy. In addition, we evaluated polarization of master gene and cytokine mRNA expression in peripheral blood CD4 + cells in the calves. The population of CD4 + CD8 + cells in thymus of the calves with thymus atrophy was lower than that of control calves. IL10 mRNA expression in peripheral blood CD4 + cells of calves with thymus atrophy was significantly lower than that of control calves. TBX21 mRNA expression in peripheral CD4 + cells of thymus atrophy calves was tended to be higher than that of the control group. In addition, FOXP3 mRNA expression in peripheral CD4 + cells of the thymus atrophy calves was tended to be lower than that of the control calves. Thymus atrophy calves exhibited chronic inflammatory disease leading, in severe situations, to conditions such as pneumonia with caseous necrosis. These severe inflammatory responses likely are due to decreases in IL10 mRNA expression, impairing control of macrophages, one of the main cell fractions of natural immunity.

Research paper thumbnail of Effects of branched-chain amino acids on immune status of young racing horses

Journal of Veterinary Medical Science, 2022

High-intensity exercise and competition are associated with depressed immune function. Young hors... more High-intensity exercise and competition are associated with depressed immune function. Young horses, which participate in high-intensity exercise and competitions, are at increased risk for the development of infectious disease due to depression of immune function. The effects of branched-chain amino acid (BCAA) supplementation on the immune status of young racing horses were evaluated, determining whether BCAA might help to avoid or reduce immune suppression during exercise and competitions. Twenty horses (10 male and 10 female) were treated with BCAA supplementation; another twenty untreated horses (10 male and 10 female) constituted control group. Peripheral blood was collected from each animal and evaluated for lymphocyte subsets, phagocytosis analysis of monocytes and granulocytes, lymphocyte proliferative response, and expression of cytokine-encoding messenger ribonucleic acids (mRNAs). The numbers of CD4 + , CD8 + , and major histocompatibility complex (MHC) class II + cells in females of the treated group were significantly higher than those in females of the control group. The lymphocyte proliferative response in female of the treated group also was significantly higher than that in females of the control group. In addition, expression of mRNAs encoding interleukin-1β (IL-1β) and interferon-γ (IFN-γ) in females of the treated group was significantly higher than that in females of the control group. There were no significant differences between males of the treated and control groups. The results of this study indicated the positive effects of BCAA supplementation in counteracting immunosuppression in young female racing horses during and following high-intensity exercise.

Research paper thumbnail of Different doses of tannin reflect a double-edged impact on broiler chicken immunity

Veterinary Immunology and Immunopathology, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of The effect of lead toxicity on male albino rats reproduction with ameliorate by vitamin E and pumpkin seeds oil

Benha Veterinary Medical Journal, 2015

Lead is an industrial pollutant that may exert specific toxic effect on mammals. The aim of study... more Lead is an industrial pollutant that may exert specific toxic effect on mammals. The aim of study was to investigate the protective effect of vit. E and pumpkin seeds oil on male reproductive system of albino rat induced by lead toxicity , the studying toxic effect of lead on body weight , testis weight, Glutathione, Glutathione-s-transferase, lipid peroxide, testosterone, lead residue , sperm gram, Nrf2 gene expression and histopathological change in testes of rat. Sixty male albino rats randomly divided into 4 groups. Group I was given olive oil 3 times a week orally, group II was given 1.5 g/L lead acetate daily in drinking water, group III was given lead acetate plus 600 mg/kg/ bwt vit. E orally 3 times a week, while group IV was given lead acetate plus 288 mg/kg/bwt pumpkin seeds oil orally 3 time a week. The experiment was extended for 8 weeks. Our results revealed significant decrease in body weight, GSH, GST, testosterone hormone level and sperm viability, and significant increase in MDA, concentration of lead, sperm abnormality and expression of Nrf2 gene in group II without any significant changes in testis and epididymis weight. Moreover, the pathological changes in testes showed focal degeneration with loss of spermatogenic series in the seminiferous tubules. All above mentioned result were significantly improved in group III & IV. In conclusion, vitamin E and pumpkin seeds oil have a protective effect on the testicular damage induced by lead.

Research paper thumbnail of Ameliorative Effect of Quercetin against Abamectin-Induced Hemato-Biochemical Alterations and Hepatorenal Oxidative Damage in Nile Tilapia, Oreochromis niloticus

Animals

Abamectin (ABM) is a common agricultural pesticide and veterinary anthelmintic drug. It can disch... more Abamectin (ABM) is a common agricultural pesticide and veterinary anthelmintic drug. It can discharge from the sites of application to aquatic systems via surface run-off or spray drift, causing harmful effects to aquatic organisms. The present study investigated the protective effect of dietary quercetin supplementation on hemato-biochemical parameters and hepato-renal oxidative stress biomarkers in Nile tilapia (Oreochromis niloticus) exposed to a sublethal dose of ABM. Fish were allocated into six equal groups. The first group was kept as a control group. The second and third groups (Q400, and Q800) were fed diets supplemented with two quercetin levels (400 and 800 mg/kg diet), respectively. The fourth group (ABM) was intoxicated with 20.73 µg/L of ABM. The fifth and sixth groups (ABM + Q400, and ABM + Q800) were fed diet supplemented with two quercetin levels (400 and 800 mg/kg diet) and simultaneously intoxicated with ABM for 60 days. The results showed that ABM significantly d...

Research paper thumbnail of Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

Comparative Biochemistry and Physiology, Part C , 2023

The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different phys... more The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different physiological responses of male African catfish, Clarias gariepinus, and the ameliorative effect of Chlorella vulgaris. The fish (160 ± 5.10 g) were exposed to 1/20 LC 50 of OXY (0.58 mg/L) for 60 consecutive days with or without coadministration of C. vulgaris (25 g/kg diet) in triplicate groups. The results revealed that chronic exposure to a sublethal level of OXY induced severe anemia and leukopenia. OXY-exposed fish experienced hypoproteinemia, marked lower AchE levels, and a significant increase in glucose, liver, and kidney function biomarkers. The DNA fragmentation of the liver increased by 15 % in fish compared to the control. On the other hand, lipid peroxidation, superoxide dismutase, and catalase activities were markedly increased in the liver and testes homogenates of the OXY-exposed fish. Meanwhile, total antioxidant capacity and glutathione S-transferase levels declined in the same tissues. Exposure to OXY induced a significant reduction in testosterone and luteinizing hormone levels and a significant increase in follicle stimulating hormone and estradiol. Meanwhile, C. vulgaris dietary supplementation succeeded in alleviating the negative impact of OXY on hematobiochemical parameters and restoring the antioxidant balance in the liver and testes. Furthermore, it ameliorated endocrine disruption and repaired sex hormone levels. In conclusion, exposure to OXY could induce systemic stress, oxidative stress, and endocrine disruption in male C. gariepinus. The dietary supplementation of C. vulgaris could be a potential protective strategy against the toxicity of OXY.