Oscar Amin - Academia.edu (original) (raw)

Papers by Oscar Amin

Research paper thumbnail of Environmental Chemistry POLYNUCLEAR AROMATIC AND CHLORINATED HYDROCARBONS IN MUSSELS FROM THE COASTAL ZONE OF USHUAIA, TIERRA DEL FUEGO, ARGENTINA

Mussels (Mytilus edulis chilensis) were collected from 12 coastal locations in Ushuaia Bay, Argen... more Mussels (Mytilus edulis chilensis) were collected from 12 coastal locations in Ushuaia Bay, Argentina, and the surrounding area in October 1999 and again in October 2003. Concentrations of polynuclear aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and selected chlorinated pesticides were determined to assess the impact of a fast-growing population in the area. Total PAH concentrations ranged from 2.24 to an extremely high concentration of 2,420 mg/g lipid measured in mussels collected near an oil jetty used to discharge to shore storage tanks. The composition of PAHs in these samples indicates that the source of these compounds inside Ushuaia Bay is predominantly petrogenic, with some pyrogenic background, whereas mostly pyrogenic-related PAHs were evident in areas outside the bay. Total concentrations of PCBs ranged between 12.8 and 8,210 ng/g lipid, with the highest concentration, detected inside Ushuaia harbor, representing a 10-fold increase when compared with h...

Research paper thumbnail of Integrated biomarker response in Mytilus chilensis exposed to untreated urban discharges along the coast of Ushuaia Bay (Beagle Channel, Argentina)

Environmental Science and Pollution Research, 2021

The short-term effects of coastal untreated effluents from Ushuaia Bay, Beagle Channel, on the bi... more The short-term effects of coastal untreated effluents from Ushuaia Bay, Beagle Channel, on the biochemical and physiological biomarkers of Mytilus chilensis were assessed. An integrated biomarker response (IBR) index was calculated as a helpful tool to represent the general stress of the experimental organisms. Cultured mussels were exposed during 96 h to three coastal sites impacted by sewage effluents. At the beginning (T0) and every 24 h, mussels were subsampled from each site and different biochemical and physiological biomarkers were determined. There was no mortality registered in the experiments during the 96 h. However, biochemical and physiological biomarkers presented significant variations. Lipid peroxidation mean levels in mussels decreased in mantle and increased in digestive gland with respect to T0 in almost all cases. Acetylcholinesterase activity was inhibited in all sites, reaching a maximal decrease of 35% with respect to T0. Catalase remained stable and glutathione-S-transferase was activated. Oxygen consumption and ammonia excretion rates increased in organisms from two sites and, consequently, O:N ratio decreased, denoting a symptom of stress. IBR values showed the existence of different stress levels between exposed and unexposed mussels. These results exhibited an alteration of the general metabolism of mussels exposed for a short period of time to untreated coastal wastewater, suggesting the use of these organisms as early indicators of changes in the environmental quality of coastal waters of Ushuaia Bay.

Research paper thumbnail of Ammonia toxicity in Southern King Crab (Lithodes santolla, Molina 1742) larvae

International Aquatic Research, 2019

Effects of ammonia on zoea I of the Southern King Crab, Lithodes santolla (Decapoda, Lithodidae) ... more Effects of ammonia on zoea I of the Southern King Crab, Lithodes santolla (Decapoda, Lithodidae) were analyzed through acute (96 h) and chronic (29 days in total) assays (seven total ammonia nitrogen (TAN) concentrations: 9, 15, 25, 41, 67, 110, and 182 mg L-1 , plus control). The estimated LC 50-96 h was 107.97 mg TAN L-1 (1.93 mg NH 3-N L-1), while the safe level of ammonia was 10.79 mg TAN L-1 (0.19 mg NH 3-N L-1). Survival was highest in the three lowest ammonia concentrations throughout 96 h (93.3%, 90% and 93.3% in 9, 15 and 25 mg TAN L-1 , respectively). In chronic assays, the percentage of survival decreased along with the exposure time and the ammonia concentration. Zoeaes mean life time tended to increase almost gradually with the increment of ammonia concentration. Mean molting time from zoea I to II was 4.06 days, while it increased from zoea II to III, and zoea III to the post-larval stage (6.00 and 8.39 more days, respectively) with ammonia concentration. The percentage of individuals that have molted in every molt stage tended to decrease while ammonia concentration increased. Therefore, the results obtained in the present study bring new information about ammonia toxicity in early stages of development of crab Lithodes santolla, an important commercial species of the Beagle Channel.

Research paper thumbnail of Starvation effects on physiological parameters and biochemical composition of the hepatopancreas of the southern king crab Lithodes santolla (Molina, 1782)

Revista de biología marina y oceanografía, 2008

Efectos del ayuno sobre parámetros fisiológicos y composición bioquímica del hepatopancreas de la... more Efectos del ayuno sobre parámetros fisiológicos y composición bioquímica del hepatopancreas de la centolla Lithodes santolla (Molina, 1782

Research paper thumbnail of Toxicidad del petróleo diesel en el primer estadio larval de la centolla (Lithodes santolla) y del centollón (Paralomis granulosa)

Revista de biología marina y oceanografía, 2002

Toxicidad del petróleo diesel en el primer estadio larval de la centolla (Lithodes santolla) y de... more Toxicidad del petróleo diesel en el primer estadio larval de la centolla (Lithodes santolla) y del centollón (Paralomis granulosa) Toxicity of diesel-oil in the first larval stage of the southern king crab (Lithodes santolla) and false southern king crab (Paralomis granulosa

Research paper thumbnail of Environmental Characterization of a Eutrophicated Semi-Enclosed System: Nutrient Budget (Encerrada Bay, Tierra del Fuego Island, Patagonia, Argentina)

Water, Air, and Soil Pollution, 2009

... environment for over 20 years. Installed sewage network service is insufficient to meet the d... more ... environment for over 20 years. Installed sewage network service is insufficient to meet the demand, especially concerning pluvial outflow channels and urban and industrial effluent treatment. The sewage network collects domestic ...

Research paper thumbnail of Feeding habits of the false southern king crab Paralomis granulosa (Lithodidae) in the Beagle Channel, Tierra del Fuego, Argentina

Scientia Marina, 1999

Stomach contents of 282 false southern king crabs (Paralomis granulosa), between 10 to 90 mm CL f... more Stomach contents of 282 false southern king crabs (Paralomis granulosa), between 10 to 90 mm CL from the Beagle Channel (Golondrina Bay and Roca Mora), Argentina, were examined by the frequency of occurrence method of analysis and by a food index. Roca Mora is an area where juveniles (<50 mm CL) dominate and in Golondrina Bay adults (>60 mm CL) are common; in this last area sexual segregation was also observed. The principal food groups for crabs of Golondrina Bay were algae, molluscs, crustaceans, bryozoans and foraminiferans; for crabs from Roca Mora the natural diet consisted of three major food groups: hydrozoans, echinoderms and foraminiferans. The relative frequency of different prey groups varied in relation to the size, season and sex. There were no significant differences in the quantity of food consumed by sexes in both areas. Generally small crabs (<40 mm CL) contained more food than large crabs (>50 mm CL). Juveniles consumed a greater amount of food during winter and spring. In summer (moulting period), juveniles had the highest vacuity index. Adults consumed minor amounts of food during autumn, before the spawning-moulting-mating period when the vacuity index was higher (spring).

Research paper thumbnail of Effects of copper on the physiological responses of the commercial crab <i>Lithodes santolla</i> (Decapoda: Anomura) larvae

Scientia Marina, 2010

Effects of copper toxicity on zoea I of Lithodes santolla (Decapoda: Anomura) were analysed. The ... more Effects of copper toxicity on zoea I of Lithodes santolla (Decapoda: Anomura) were analysed. The 96-h LC 50 was estimated, resulting in 298.5 µg L-1. Groups of larvae were exposed to sublethal concentrations (40, 80 and 160 µg L-1) for 96 h. Oxygen consumption, ammonia excretion, O:N atomic ratio, lipid peroxidation (LPO) and body water content were measured. Oxygen consumption of treated groups (mean 46.92 ± 8.03 µg-atom O 2 h-1 mg-1) did not differ significantly with control. Ammonia excretion decreased by 60% at higher Cu concentration (1.61 ± 0.65 µg-atom N-NH 3 h-1 mg-1), leading to a 117% increase in the O:N ratio. LPO values during the exposure time were higher in all treatments than in the controls. The water content was significantly higher in treatments than in controls. The highest concentration assayed, which represents about 50% of 96-h LC 50 , had evident effects on the parameters analysed. The values of copper in water reported for the coastal zone of Ushuaia bay exceed the value established by the United States Environmental Protection Agency (USEPA) for ambient water quality criteria. Therefore, the results obtained in the present study are a contribution to the study of potential effects of copper as a common stressor in the first larval stage of this commercial species of the Beagle Channel.

Research paper thumbnail of Heavy metal concentrations and biomarkers of oxidative stress in native mussels (Mytilus edulis chilensis) from Beagle Channel coast (Tierra del Fuego, Argentina)

Marine Pollution Bulletin, 2011

The aim of this study was to evaluate the usefulness of oxidative stress biomarkers of pollution ... more The aim of this study was to evaluate the usefulness of oxidative stress biomarkers of pollution in native mussels Mytilus edulis chilensis from the Beagle Channel. Spatial and seasonal variations of catalase, glutathione-S-transferase and lipid peroxidation in gills and digestive gland were analyzed in relation to environmental parameters, heavy metals in sediment and in tissue. Four sites with anthropogenic impact and a control site were selected and monitored during the four seasons of 2007. We found significant differences among sites in concentrations of dissolved nutrients and heavy metals in sediments, with the highest values recorded at sites with anthropogenic pressure. Different patterns were observed between concentrations of metals in tissues and in sediments suggesting differences in bioavailability. There were also significant differences in biomarker responses among sites, despite the strong seasonal variability. Our results showed relatively moderate levels of pollution in the study area as a result of urban influences.

Research paper thumbnail of Assessment of recent sediment influence in an urban polluted subantarctic coastal ecosystem. Beagle Channel (Southern Argentina)

Marine Pollution Bulletin, 2011

Research paper thumbnail of Responses of the resident rocky crab (Halicarcinus planatus, Decapoda) to natural stressors and effluent discharges in Ushuaia Bay, Tierra del Fuego, Argentina

Journal of Experimental Marine Biology and Ecology, 2012

Ushuaia City has been growing since 1980 and industrial and domestic effluents have been discharg... more Ushuaia City has been growing since 1980 and industrial and domestic effluents have been discharged along its coasts. The present study evaluates the acute exposure of the rocky crab Halicarcinus planatus to three environmental stressors (salinity, pH and ammonia) and to in situ coastal whole effluents (Industrial Zone (IZ), Yacht Club (YC) and Encerrada Bay (EB)). Neither mortalities nor molting events were recorded during the study period. The highest physiological rates were at salinity 25, at pH 9.5 and at 3 mg N-NH 3 L −1 (174.38± 17.76 μg O 2 h −1 g −1 and 12.80 ± 4.54 μg N-NH 3 h −1 g −1 ; 199.45 ± 11.86 μg O 2 h −1 g − 1 and 27.82 ± 6.88 μg N-NH 3 h −1 g −1 ; and 232± 43.5 μg O 2 h −1 g −1 and 26.29± 3.42 μg N-NH 3 h −1 g −1 , respectively). Crabs exposed to the studied areas also showed a tendency to increase physiological parameters. Acetylcholynesterase (AChE) activity showed maximal inhibition in organisms from YC (0.14± 0.07 nmol min −1 mg protein −1). H. planatus showed responses to both specific and complex environmental stressors and its use as a suitable bioindicator of environmental changes is discussed.

Research paper thumbnail of Effects of lead and cadmium on hatching of the southern king crabLithodes santolla(Decapoda, Anomura)

Invertebrate Reproduction & Development, 1998

Eggs of the king crab Lithodes santolla were exposed to cadmium (0.2 and 2 mg/1) and lead (0.16 a... more Eggs of the king crab Lithodes santolla were exposed to cadmium (0.2 and 2 mg/1) and lead (0.16 and 1.6 mg/1) for 30 days. Concentrations used represented 1/10 and 1 of the respective LC50 values for zoea I of the studied species. Proportion of larvae hatching, meantime of eclosion and proportion of abnormal larvae were recorded. Abnormalities detected were hydropsy;

Research paper thumbnail of Organotin compounds in sediments of three coastal environments from the Patagonian shore, Argentina

International Journal of Environment and Waste Management, 2011

One of the most toxic compounds that have been introduced into the marine environment is Tributyl... more One of the most toxic compounds that have been introduced into the marine environment is Tributyltin (TBT) used as a biocide in antifouling paints. The aim of this study was to obtain the first information of TBT pollution in sediments of three environments of the Argentinean coastline. Sediments from the Bahía Blanca estuary, San Antonio Bay and Ushuaia Bay were sampled to determine TBT and dibutyltin (DBT) concentration. The butyltin compounds were identified and quantified by gas chromatography and mass spectrometry Recently, he has published about heavy metal and hydrocarbon pollution. Jorge Marcovecchio got Biological Degree from UNMdP (Mar del Plata University). He got PhD in Biological Sciences oriented to Chemical Oceanography from the same university. He became Main Researcher for CONICET (Argentina) at IADO (Argentinean Institute of Oceanography). He leads a group of investigation on biogeochemical cycles of nutrients and metals in coastal and estuarine systems, coastal management, ecotoxicology of metals, phytoplankton productivity in estuarine environments, environmental quality. He teaches both undergraduate and postgraduate courses at UTN

Research paper thumbnail of Polynuclear aromatic and chlorinated hydrocarbons in mussels from the coastal zone of Ushuaia, Tierra del Fuego, Argentina

Environmental Toxicology and Chemistry, 2011

Mussels (Mytilus edulis chilensis) were collected from 12 coastal locations in Ushuaia Bay, Argen... more Mussels (Mytilus edulis chilensis) were collected from 12 coastal locations in Ushuaia Bay, Argentina, and the surrounding area in October 1999 and again in October 2003. Concentrations of polynuclear aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and selected chlorinated pesticides were determined to assess the impact of a fast-growing population in the area. Total PAH concentrations ranged from 2.24 to an extremely high concentration of 2,420 µg/g lipid measured in mussels collected near an oil jetty used to discharge to shore storage tanks. The composition of PAHs in these samples indicates that the source of these compounds inside Ushuaia Bay is predominantly petrogenic, with some pyrogenic background, whereas mostly pyrogenic-related PAHs were evident in areas outside the bay. Total concentrations of PCBs ranged between 12.8 and 8,210 ng/g lipid, with the highest concentration, detected inside Ushuaia harbor, representing a 10-fold increase when compared with historical data. Chlorinated pesticides were detected at comparatively lower concentrations, with 4-4&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;- 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene being the most common. The aggressive increase in population and related activities observed in the city of Ushuaia over the last two decades might have affected the environmental quality of the local bay. Moreover, the oceanographic and atmospheric conditions existing in Ushuaia Bay and surrounding areas may favor the accumulation and long-term presence of these organic pollutants in all compartments of this fragile environment.

Research paper thumbnail of Assessment of land influence on a high-latitude marine coastal system: Tierra del Fuego, southernmost Argentina

Environmental Monitoring and Assessment, 2010

The study deals with the determination of physico-chemical parameters, inorganic nutrients, parti... more The study deals with the determination of physico-chemical parameters, inorganic nutrients, particulate organic matter, and photosynthetic pigments on a monthly basis during an annual cycle from nine sampling sites of the coastal zone of a high-latitude ecosystem (Tierra del Fuego, Argentina). Nitrites and phosphates concentrations were similar to other systems of the south Atlantic coast (median, 0.30 and 1.02 μM, respectively), while nitrates were higher in all sampling periods (median, 45.37 μM), and silicates were significantly smaller (median, 7.76

Research paper thumbnail of Biochemical response of amphipods (Gammarid: Paramorea) in a sediment laboratory exposure from Ushuaia Bay, Beagle Channel

Ecotoxicology and Environmental Safety, 2011

A coastal system (Ushuaia Bay, Argentina) impacted by anthropogenic activities was studied by the... more A coastal system (Ushuaia Bay, Argentina) impacted by anthropogenic activities was studied by the response of local amphipods (Parmorea sp., Gammaridae) to the exposure of coastal sediments in a laboratory assay. Four coastal areas with different loadings of contaminants and one considered as reference were studied. Organic matter, carbohydrates, proteins and heavy metals were measured in sediment samples. Organisms were exposed to sediments for seven days and catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE) and lipid peroxidation (LPO) were measured afterward. Amphipods exhibited an activation of GST and inhibition of AChE in most impacted areas. Principal Component Analysis (PCA) was conducted in order to associate the biological responses with sediment metal concentration and its eutrophicated status. Levels of Cd and Cr were associated with the inhibition of AChE and with the enhancement of GST. CAT and LPO were enhanced in most areas, but no link was found with the contaminants studied by PCA, suggesting that other parameters present in sediments not included in the PCA affect the amphipods. The most impacted area corresponds to Nautical Club station, with a highly eutrophicated status and high content of metals, where amphipods after the exposure were affected in a biochemical level.

Research paper thumbnail of Heavy metals monitoring in the southernmost mussel farm of the world (Beagle Channel, Argentina)

Ecotoxicology and Environmental Safety, 2010

Water quality surrounding the mussel farm of Mytilus edulis chilensis at Brown Bay (Beagle Channe... more Water quality surrounding the mussel farm of Mytilus edulis chilensis at Brown Bay (Beagle Channel) was evaluated. The levels of five heavy metals in sediment and in gill and digestive gland of mussels were examined to consider potential risks to human health. Cd showed the highest enrichment factor in relation to its level in Earth crust (3.85-21.58), which could be related to an upwelling phenomenon. A seasonal trend was found regarding metal bioaccumulation, being higher in winter than in summer. The bioaccumulation pattern in gill was Zn&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Fe&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Cu&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Cd, meanwhile in digestive gland was Fe&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Zn&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Cu&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Cd. Despite Pb was measured in sediment (15.59-23.91 microg/g dw), it was not available for being incorporated by mussels. In all cases it was below the detection limit (2.37 microg/g dw). With regard to human consumption of mussels from Brown Bay, none of the elements analyzed should cause concern for consumers. Values measured in tissue mussels were below the limit of 10 microg/g dw for Cd and Pb established by SENASA for molluscs. Considering that studied mussels are for human consumption and the relatively high levels of metals in sediment may vary their availability if physical parameters changes, periodical monitoring must be carried out to avoid human risks and to produce food in a responsible manner that complies with the food safety standards.

Research paper thumbnail of Evaluation of sublethal biomarkers in Litopenaeus vannamei on foodborne exposure to methyl parathion

Ecotoxicology and Environmental Safety, 2005

Sublethal effects of foodborne exposure to methyl parathion (0.62 and 1.31 microg methyl parathio... more Sublethal effects of foodborne exposure to methyl parathion (0.62 and 1.31 microg methyl parathion*g(-1) dry weight of food) on juveniles of Litopenaeus vannamei using integrated biochemical (acetylcholinesterase (AChE) and ATPases) and physiological (feeding rate (FR), egestion rate (ER), and hepatosomatic index (HI)) biomarkers were evaluated. The HI was significantly higher in controls than in pesticide treatments. The FR was significantly lower in controls than in pesticide treatments while no significant differences were detected in the ER. AChE activity was significantly higher in controls than in pesticide treatments (control = 0.11 +/- 0.02; solvent control = 0.11 +/- 0.03; 0.62 = 0.07 +/- 0.01; 1.31 = 0.08 +/- 0.02 microM*min(-1)*mgprotein(-1)). The total-ATPase activity was significantly lower in controls than in pesticide treatments (control=77.90+/-12.41; solvent control = 83.69 +/- 22.05; 0.62 = 110.03 +/- 22.17; 1.31 = 121.54 +/- 19.84 microM P(i)*h(-1)*mgprotein(-1)). The Mg(2+)-ATPase activity was significantly higher in treatments than in controls (control = 65.14+/-10.76; solvent control = 75.12 +/- 21.10; 0.62 = 100.53 +/- 20.97; 1.31 = 108.94 +/- 17.26 microM P(i)*h(-1)*mgprotein(-1)). Finally, the results obtained for the Na(+)/K(+)-ATPase activity were significantly higher in control and in 1.31 than in solvent control and in 0.62 (control = 14.06+/-2.63; solvent control=7.30 +/- 4.13; 0.62 = 7.60 +/- 3.81; 1.31 = 13.42 +/- 2.88 microM P(i)*h(-1)*mgprotein(-1)). The results in this study showed that pulse exposures to methyl parathion via food could elicit measurable effects on the marine shrimp L. vannamei, indicating that foodborne exposure can be a reliable toxicological procedure and, if combined with pulse exposures, could also simulate more realistic exposure scenarios.

Research paper thumbnail of Heavy metal toxicity in Exosphaeroma gigas (Crustacea, Isopoda) from the coastal zone of Beagle Channel

Ecotoxicology and Environmental Safety, 2007

Acute toxicity of copper, cadmium, and zinc on isopod Exosphaeroma gigas was evaluated at 20 per ... more Acute toxicity of copper, cadmium, and zinc on isopod Exosphaeroma gigas was evaluated at 20 per thousand and 30 per thousand salinity. Six concentrations were assayed to estimate effective concentration of the toxicant that affects 50% of tested animal (EC50), while physiological responses and bioaccumulation were determined at 0.42 and 1.95 mg L(-1) of each metal. The following toxicity orders were obtained: Cd&amp;amp;amp;amp;amp;amp;amp;amp;gt;or=Cu&amp;amp;amp;amp;amp;amp;amp;amp;gt;Zn at 20 per thousand salinity and Cu&amp;amp;amp;amp;amp;amp;amp;amp;gt;Zn&amp;amp;amp;amp;amp;amp;amp;amp;gt;or=Cd at 30 per thousand salinity. Copper treatments showed a reduction in oxygen consumption at 30 per thousand salinity, while an opposite trend was observed at 20 per thousand salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.42(20 per thousand) mg L(-1). Ammonia excretion was generally higher in treatments than control at 20 per thousand salinity. At 30 per thousand salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations.

Research paper thumbnail of Biomarkers and heavy metal bioaccumulation in mussels transplanted to coastal waters of the Beagle Channel

Ecotoxicology and Environmental Safety, 2010

Mussels coming from a mussel farm at Brown Bay (Beagle Channel) were transplanted to four sites i... more Mussels coming from a mussel farm at Brown Bay (Beagle Channel) were transplanted to four sites inside Ushuaia Bay for 2 and 4 weeks. The objective of this study was to assess the quality of coastal waters of Ushuaia Bay by measuring catalase activity, lipid peroxidation, total lipid content, bioaccumulation of heavy metals and condition index in transplanted mussel Mytilus edulis chilensis. Biomarkers except condition index showed significant differences among exposure times as well as among tissues. Digestive gland presented the highest catalase activity, malondialdehyde level and total lipid content. Digestive gland also was the main target tissue of accumulation of iron and copper, while gill accumulated the highest levels of zinc. A principal component analyzes with the whole set of data allowed to separate stations based on physicochemical conditions and biochemical responses of each studied area.

Research paper thumbnail of Environmental Chemistry POLYNUCLEAR AROMATIC AND CHLORINATED HYDROCARBONS IN MUSSELS FROM THE COASTAL ZONE OF USHUAIA, TIERRA DEL FUEGO, ARGENTINA

Mussels (Mytilus edulis chilensis) were collected from 12 coastal locations in Ushuaia Bay, Argen... more Mussels (Mytilus edulis chilensis) were collected from 12 coastal locations in Ushuaia Bay, Argentina, and the surrounding area in October 1999 and again in October 2003. Concentrations of polynuclear aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and selected chlorinated pesticides were determined to assess the impact of a fast-growing population in the area. Total PAH concentrations ranged from 2.24 to an extremely high concentration of 2,420 mg/g lipid measured in mussels collected near an oil jetty used to discharge to shore storage tanks. The composition of PAHs in these samples indicates that the source of these compounds inside Ushuaia Bay is predominantly petrogenic, with some pyrogenic background, whereas mostly pyrogenic-related PAHs were evident in areas outside the bay. Total concentrations of PCBs ranged between 12.8 and 8,210 ng/g lipid, with the highest concentration, detected inside Ushuaia harbor, representing a 10-fold increase when compared with h...

Research paper thumbnail of Integrated biomarker response in Mytilus chilensis exposed to untreated urban discharges along the coast of Ushuaia Bay (Beagle Channel, Argentina)

Environmental Science and Pollution Research, 2021

The short-term effects of coastal untreated effluents from Ushuaia Bay, Beagle Channel, on the bi... more The short-term effects of coastal untreated effluents from Ushuaia Bay, Beagle Channel, on the biochemical and physiological biomarkers of Mytilus chilensis were assessed. An integrated biomarker response (IBR) index was calculated as a helpful tool to represent the general stress of the experimental organisms. Cultured mussels were exposed during 96 h to three coastal sites impacted by sewage effluents. At the beginning (T0) and every 24 h, mussels were subsampled from each site and different biochemical and physiological biomarkers were determined. There was no mortality registered in the experiments during the 96 h. However, biochemical and physiological biomarkers presented significant variations. Lipid peroxidation mean levels in mussels decreased in mantle and increased in digestive gland with respect to T0 in almost all cases. Acetylcholinesterase activity was inhibited in all sites, reaching a maximal decrease of 35% with respect to T0. Catalase remained stable and glutathione-S-transferase was activated. Oxygen consumption and ammonia excretion rates increased in organisms from two sites and, consequently, O:N ratio decreased, denoting a symptom of stress. IBR values showed the existence of different stress levels between exposed and unexposed mussels. These results exhibited an alteration of the general metabolism of mussels exposed for a short period of time to untreated coastal wastewater, suggesting the use of these organisms as early indicators of changes in the environmental quality of coastal waters of Ushuaia Bay.

Research paper thumbnail of Ammonia toxicity in Southern King Crab (Lithodes santolla, Molina 1742) larvae

International Aquatic Research, 2019

Effects of ammonia on zoea I of the Southern King Crab, Lithodes santolla (Decapoda, Lithodidae) ... more Effects of ammonia on zoea I of the Southern King Crab, Lithodes santolla (Decapoda, Lithodidae) were analyzed through acute (96 h) and chronic (29 days in total) assays (seven total ammonia nitrogen (TAN) concentrations: 9, 15, 25, 41, 67, 110, and 182 mg L-1 , plus control). The estimated LC 50-96 h was 107.97 mg TAN L-1 (1.93 mg NH 3-N L-1), while the safe level of ammonia was 10.79 mg TAN L-1 (0.19 mg NH 3-N L-1). Survival was highest in the three lowest ammonia concentrations throughout 96 h (93.3%, 90% and 93.3% in 9, 15 and 25 mg TAN L-1 , respectively). In chronic assays, the percentage of survival decreased along with the exposure time and the ammonia concentration. Zoeaes mean life time tended to increase almost gradually with the increment of ammonia concentration. Mean molting time from zoea I to II was 4.06 days, while it increased from zoea II to III, and zoea III to the post-larval stage (6.00 and 8.39 more days, respectively) with ammonia concentration. The percentage of individuals that have molted in every molt stage tended to decrease while ammonia concentration increased. Therefore, the results obtained in the present study bring new information about ammonia toxicity in early stages of development of crab Lithodes santolla, an important commercial species of the Beagle Channel.

Research paper thumbnail of Starvation effects on physiological parameters and biochemical composition of the hepatopancreas of the southern king crab Lithodes santolla (Molina, 1782)

Revista de biología marina y oceanografía, 2008

Efectos del ayuno sobre parámetros fisiológicos y composición bioquímica del hepatopancreas de la... more Efectos del ayuno sobre parámetros fisiológicos y composición bioquímica del hepatopancreas de la centolla Lithodes santolla (Molina, 1782

Research paper thumbnail of Toxicidad del petróleo diesel en el primer estadio larval de la centolla (Lithodes santolla) y del centollón (Paralomis granulosa)

Revista de biología marina y oceanografía, 2002

Toxicidad del petróleo diesel en el primer estadio larval de la centolla (Lithodes santolla) y de... more Toxicidad del petróleo diesel en el primer estadio larval de la centolla (Lithodes santolla) y del centollón (Paralomis granulosa) Toxicity of diesel-oil in the first larval stage of the southern king crab (Lithodes santolla) and false southern king crab (Paralomis granulosa

Research paper thumbnail of Environmental Characterization of a Eutrophicated Semi-Enclosed System: Nutrient Budget (Encerrada Bay, Tierra del Fuego Island, Patagonia, Argentina)

Water, Air, and Soil Pollution, 2009

... environment for over 20 years. Installed sewage network service is insufficient to meet the d... more ... environment for over 20 years. Installed sewage network service is insufficient to meet the demand, especially concerning pluvial outflow channels and urban and industrial effluent treatment. The sewage network collects domestic ...

Research paper thumbnail of Feeding habits of the false southern king crab Paralomis granulosa (Lithodidae) in the Beagle Channel, Tierra del Fuego, Argentina

Scientia Marina, 1999

Stomach contents of 282 false southern king crabs (Paralomis granulosa), between 10 to 90 mm CL f... more Stomach contents of 282 false southern king crabs (Paralomis granulosa), between 10 to 90 mm CL from the Beagle Channel (Golondrina Bay and Roca Mora), Argentina, were examined by the frequency of occurrence method of analysis and by a food index. Roca Mora is an area where juveniles (<50 mm CL) dominate and in Golondrina Bay adults (>60 mm CL) are common; in this last area sexual segregation was also observed. The principal food groups for crabs of Golondrina Bay were algae, molluscs, crustaceans, bryozoans and foraminiferans; for crabs from Roca Mora the natural diet consisted of three major food groups: hydrozoans, echinoderms and foraminiferans. The relative frequency of different prey groups varied in relation to the size, season and sex. There were no significant differences in the quantity of food consumed by sexes in both areas. Generally small crabs (<40 mm CL) contained more food than large crabs (>50 mm CL). Juveniles consumed a greater amount of food during winter and spring. In summer (moulting period), juveniles had the highest vacuity index. Adults consumed minor amounts of food during autumn, before the spawning-moulting-mating period when the vacuity index was higher (spring).

Research paper thumbnail of Effects of copper on the physiological responses of the commercial crab <i>Lithodes santolla</i> (Decapoda: Anomura) larvae

Scientia Marina, 2010

Effects of copper toxicity on zoea I of Lithodes santolla (Decapoda: Anomura) were analysed. The ... more Effects of copper toxicity on zoea I of Lithodes santolla (Decapoda: Anomura) were analysed. The 96-h LC 50 was estimated, resulting in 298.5 µg L-1. Groups of larvae were exposed to sublethal concentrations (40, 80 and 160 µg L-1) for 96 h. Oxygen consumption, ammonia excretion, O:N atomic ratio, lipid peroxidation (LPO) and body water content were measured. Oxygen consumption of treated groups (mean 46.92 ± 8.03 µg-atom O 2 h-1 mg-1) did not differ significantly with control. Ammonia excretion decreased by 60% at higher Cu concentration (1.61 ± 0.65 µg-atom N-NH 3 h-1 mg-1), leading to a 117% increase in the O:N ratio. LPO values during the exposure time were higher in all treatments than in the controls. The water content was significantly higher in treatments than in controls. The highest concentration assayed, which represents about 50% of 96-h LC 50 , had evident effects on the parameters analysed. The values of copper in water reported for the coastal zone of Ushuaia bay exceed the value established by the United States Environmental Protection Agency (USEPA) for ambient water quality criteria. Therefore, the results obtained in the present study are a contribution to the study of potential effects of copper as a common stressor in the first larval stage of this commercial species of the Beagle Channel.

Research paper thumbnail of Heavy metal concentrations and biomarkers of oxidative stress in native mussels (Mytilus edulis chilensis) from Beagle Channel coast (Tierra del Fuego, Argentina)

Marine Pollution Bulletin, 2011

The aim of this study was to evaluate the usefulness of oxidative stress biomarkers of pollution ... more The aim of this study was to evaluate the usefulness of oxidative stress biomarkers of pollution in native mussels Mytilus edulis chilensis from the Beagle Channel. Spatial and seasonal variations of catalase, glutathione-S-transferase and lipid peroxidation in gills and digestive gland were analyzed in relation to environmental parameters, heavy metals in sediment and in tissue. Four sites with anthropogenic impact and a control site were selected and monitored during the four seasons of 2007. We found significant differences among sites in concentrations of dissolved nutrients and heavy metals in sediments, with the highest values recorded at sites with anthropogenic pressure. Different patterns were observed between concentrations of metals in tissues and in sediments suggesting differences in bioavailability. There were also significant differences in biomarker responses among sites, despite the strong seasonal variability. Our results showed relatively moderate levels of pollution in the study area as a result of urban influences.

Research paper thumbnail of Assessment of recent sediment influence in an urban polluted subantarctic coastal ecosystem. Beagle Channel (Southern Argentina)

Marine Pollution Bulletin, 2011

Research paper thumbnail of Responses of the resident rocky crab (Halicarcinus planatus, Decapoda) to natural stressors and effluent discharges in Ushuaia Bay, Tierra del Fuego, Argentina

Journal of Experimental Marine Biology and Ecology, 2012

Ushuaia City has been growing since 1980 and industrial and domestic effluents have been discharg... more Ushuaia City has been growing since 1980 and industrial and domestic effluents have been discharged along its coasts. The present study evaluates the acute exposure of the rocky crab Halicarcinus planatus to three environmental stressors (salinity, pH and ammonia) and to in situ coastal whole effluents (Industrial Zone (IZ), Yacht Club (YC) and Encerrada Bay (EB)). Neither mortalities nor molting events were recorded during the study period. The highest physiological rates were at salinity 25, at pH 9.5 and at 3 mg N-NH 3 L −1 (174.38± 17.76 μg O 2 h −1 g −1 and 12.80 ± 4.54 μg N-NH 3 h −1 g −1 ; 199.45 ± 11.86 μg O 2 h −1 g − 1 and 27.82 ± 6.88 μg N-NH 3 h −1 g −1 ; and 232± 43.5 μg O 2 h −1 g −1 and 26.29± 3.42 μg N-NH 3 h −1 g −1 , respectively). Crabs exposed to the studied areas also showed a tendency to increase physiological parameters. Acetylcholynesterase (AChE) activity showed maximal inhibition in organisms from YC (0.14± 0.07 nmol min −1 mg protein −1). H. planatus showed responses to both specific and complex environmental stressors and its use as a suitable bioindicator of environmental changes is discussed.

Research paper thumbnail of Effects of lead and cadmium on hatching of the southern king crabLithodes santolla(Decapoda, Anomura)

Invertebrate Reproduction & Development, 1998

Eggs of the king crab Lithodes santolla were exposed to cadmium (0.2 and 2 mg/1) and lead (0.16 a... more Eggs of the king crab Lithodes santolla were exposed to cadmium (0.2 and 2 mg/1) and lead (0.16 and 1.6 mg/1) for 30 days. Concentrations used represented 1/10 and 1 of the respective LC50 values for zoea I of the studied species. Proportion of larvae hatching, meantime of eclosion and proportion of abnormal larvae were recorded. Abnormalities detected were hydropsy;

Research paper thumbnail of Organotin compounds in sediments of three coastal environments from the Patagonian shore, Argentina

International Journal of Environment and Waste Management, 2011

One of the most toxic compounds that have been introduced into the marine environment is Tributyl... more One of the most toxic compounds that have been introduced into the marine environment is Tributyltin (TBT) used as a biocide in antifouling paints. The aim of this study was to obtain the first information of TBT pollution in sediments of three environments of the Argentinean coastline. Sediments from the Bahía Blanca estuary, San Antonio Bay and Ushuaia Bay were sampled to determine TBT and dibutyltin (DBT) concentration. The butyltin compounds were identified and quantified by gas chromatography and mass spectrometry Recently, he has published about heavy metal and hydrocarbon pollution. Jorge Marcovecchio got Biological Degree from UNMdP (Mar del Plata University). He got PhD in Biological Sciences oriented to Chemical Oceanography from the same university. He became Main Researcher for CONICET (Argentina) at IADO (Argentinean Institute of Oceanography). He leads a group of investigation on biogeochemical cycles of nutrients and metals in coastal and estuarine systems, coastal management, ecotoxicology of metals, phytoplankton productivity in estuarine environments, environmental quality. He teaches both undergraduate and postgraduate courses at UTN

Research paper thumbnail of Polynuclear aromatic and chlorinated hydrocarbons in mussels from the coastal zone of Ushuaia, Tierra del Fuego, Argentina

Environmental Toxicology and Chemistry, 2011

Mussels (Mytilus edulis chilensis) were collected from 12 coastal locations in Ushuaia Bay, Argen... more Mussels (Mytilus edulis chilensis) were collected from 12 coastal locations in Ushuaia Bay, Argentina, and the surrounding area in October 1999 and again in October 2003. Concentrations of polynuclear aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and selected chlorinated pesticides were determined to assess the impact of a fast-growing population in the area. Total PAH concentrations ranged from 2.24 to an extremely high concentration of 2,420 µg/g lipid measured in mussels collected near an oil jetty used to discharge to shore storage tanks. The composition of PAHs in these samples indicates that the source of these compounds inside Ushuaia Bay is predominantly petrogenic, with some pyrogenic background, whereas mostly pyrogenic-related PAHs were evident in areas outside the bay. Total concentrations of PCBs ranged between 12.8 and 8,210 ng/g lipid, with the highest concentration, detected inside Ushuaia harbor, representing a 10-fold increase when compared with historical data. Chlorinated pesticides were detected at comparatively lower concentrations, with 4-4&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;- 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene being the most common. The aggressive increase in population and related activities observed in the city of Ushuaia over the last two decades might have affected the environmental quality of the local bay. Moreover, the oceanographic and atmospheric conditions existing in Ushuaia Bay and surrounding areas may favor the accumulation and long-term presence of these organic pollutants in all compartments of this fragile environment.

Research paper thumbnail of Assessment of land influence on a high-latitude marine coastal system: Tierra del Fuego, southernmost Argentina

Environmental Monitoring and Assessment, 2010

The study deals with the determination of physico-chemical parameters, inorganic nutrients, parti... more The study deals with the determination of physico-chemical parameters, inorganic nutrients, particulate organic matter, and photosynthetic pigments on a monthly basis during an annual cycle from nine sampling sites of the coastal zone of a high-latitude ecosystem (Tierra del Fuego, Argentina). Nitrites and phosphates concentrations were similar to other systems of the south Atlantic coast (median, 0.30 and 1.02 μM, respectively), while nitrates were higher in all sampling periods (median, 45.37 μM), and silicates were significantly smaller (median, 7.76

Research paper thumbnail of Biochemical response of amphipods (Gammarid: Paramorea) in a sediment laboratory exposure from Ushuaia Bay, Beagle Channel

Ecotoxicology and Environmental Safety, 2011

A coastal system (Ushuaia Bay, Argentina) impacted by anthropogenic activities was studied by the... more A coastal system (Ushuaia Bay, Argentina) impacted by anthropogenic activities was studied by the response of local amphipods (Parmorea sp., Gammaridae) to the exposure of coastal sediments in a laboratory assay. Four coastal areas with different loadings of contaminants and one considered as reference were studied. Organic matter, carbohydrates, proteins and heavy metals were measured in sediment samples. Organisms were exposed to sediments for seven days and catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE) and lipid peroxidation (LPO) were measured afterward. Amphipods exhibited an activation of GST and inhibition of AChE in most impacted areas. Principal Component Analysis (PCA) was conducted in order to associate the biological responses with sediment metal concentration and its eutrophicated status. Levels of Cd and Cr were associated with the inhibition of AChE and with the enhancement of GST. CAT and LPO were enhanced in most areas, but no link was found with the contaminants studied by PCA, suggesting that other parameters present in sediments not included in the PCA affect the amphipods. The most impacted area corresponds to Nautical Club station, with a highly eutrophicated status and high content of metals, where amphipods after the exposure were affected in a biochemical level.

Research paper thumbnail of Heavy metals monitoring in the southernmost mussel farm of the world (Beagle Channel, Argentina)

Ecotoxicology and Environmental Safety, 2010

Water quality surrounding the mussel farm of Mytilus edulis chilensis at Brown Bay (Beagle Channe... more Water quality surrounding the mussel farm of Mytilus edulis chilensis at Brown Bay (Beagle Channel) was evaluated. The levels of five heavy metals in sediment and in gill and digestive gland of mussels were examined to consider potential risks to human health. Cd showed the highest enrichment factor in relation to its level in Earth crust (3.85-21.58), which could be related to an upwelling phenomenon. A seasonal trend was found regarding metal bioaccumulation, being higher in winter than in summer. The bioaccumulation pattern in gill was Zn&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Fe&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Cu&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Cd, meanwhile in digestive gland was Fe&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Zn&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Cu&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Cd. Despite Pb was measured in sediment (15.59-23.91 microg/g dw), it was not available for being incorporated by mussels. In all cases it was below the detection limit (2.37 microg/g dw). With regard to human consumption of mussels from Brown Bay, none of the elements analyzed should cause concern for consumers. Values measured in tissue mussels were below the limit of 10 microg/g dw for Cd and Pb established by SENASA for molluscs. Considering that studied mussels are for human consumption and the relatively high levels of metals in sediment may vary their availability if physical parameters changes, periodical monitoring must be carried out to avoid human risks and to produce food in a responsible manner that complies with the food safety standards.

Research paper thumbnail of Evaluation of sublethal biomarkers in Litopenaeus vannamei on foodborne exposure to methyl parathion

Ecotoxicology and Environmental Safety, 2005

Sublethal effects of foodborne exposure to methyl parathion (0.62 and 1.31 microg methyl parathio... more Sublethal effects of foodborne exposure to methyl parathion (0.62 and 1.31 microg methyl parathion*g(-1) dry weight of food) on juveniles of Litopenaeus vannamei using integrated biochemical (acetylcholinesterase (AChE) and ATPases) and physiological (feeding rate (FR), egestion rate (ER), and hepatosomatic index (HI)) biomarkers were evaluated. The HI was significantly higher in controls than in pesticide treatments. The FR was significantly lower in controls than in pesticide treatments while no significant differences were detected in the ER. AChE activity was significantly higher in controls than in pesticide treatments (control = 0.11 +/- 0.02; solvent control = 0.11 +/- 0.03; 0.62 = 0.07 +/- 0.01; 1.31 = 0.08 +/- 0.02 microM*min(-1)*mgprotein(-1)). The total-ATPase activity was significantly lower in controls than in pesticide treatments (control=77.90+/-12.41; solvent control = 83.69 +/- 22.05; 0.62 = 110.03 +/- 22.17; 1.31 = 121.54 +/- 19.84 microM P(i)*h(-1)*mgprotein(-1)). The Mg(2+)-ATPase activity was significantly higher in treatments than in controls (control = 65.14+/-10.76; solvent control = 75.12 +/- 21.10; 0.62 = 100.53 +/- 20.97; 1.31 = 108.94 +/- 17.26 microM P(i)*h(-1)*mgprotein(-1)). Finally, the results obtained for the Na(+)/K(+)-ATPase activity were significantly higher in control and in 1.31 than in solvent control and in 0.62 (control = 14.06+/-2.63; solvent control=7.30 +/- 4.13; 0.62 = 7.60 +/- 3.81; 1.31 = 13.42 +/- 2.88 microM P(i)*h(-1)*mgprotein(-1)). The results in this study showed that pulse exposures to methyl parathion via food could elicit measurable effects on the marine shrimp L. vannamei, indicating that foodborne exposure can be a reliable toxicological procedure and, if combined with pulse exposures, could also simulate more realistic exposure scenarios.

Research paper thumbnail of Heavy metal toxicity in Exosphaeroma gigas (Crustacea, Isopoda) from the coastal zone of Beagle Channel

Ecotoxicology and Environmental Safety, 2007

Acute toxicity of copper, cadmium, and zinc on isopod Exosphaeroma gigas was evaluated at 20 per ... more Acute toxicity of copper, cadmium, and zinc on isopod Exosphaeroma gigas was evaluated at 20 per thousand and 30 per thousand salinity. Six concentrations were assayed to estimate effective concentration of the toxicant that affects 50% of tested animal (EC50), while physiological responses and bioaccumulation were determined at 0.42 and 1.95 mg L(-1) of each metal. The following toxicity orders were obtained: Cd&amp;amp;amp;amp;amp;amp;amp;amp;gt;or=Cu&amp;amp;amp;amp;amp;amp;amp;amp;gt;Zn at 20 per thousand salinity and Cu&amp;amp;amp;amp;amp;amp;amp;amp;gt;Zn&amp;amp;amp;amp;amp;amp;amp;amp;gt;or=Cd at 30 per thousand salinity. Copper treatments showed a reduction in oxygen consumption at 30 per thousand salinity, while an opposite trend was observed at 20 per thousand salinity. Zinc caused dissimilar effects, while in cadmium significant reduction was only registered in 0.42(20 per thousand) mg L(-1). Ammonia excretion was generally higher in treatments than control at 20 per thousand salinity. At 30 per thousand salinity, excretion did not change in relation to control or diminished. In general terms, O:N atomic ratios indicated a preponderant protein metabolism. Bioaccumulation of assayed metals was higher at lowest salinity and increased with increasing toxic concentrations.

Research paper thumbnail of Biomarkers and heavy metal bioaccumulation in mussels transplanted to coastal waters of the Beagle Channel

Ecotoxicology and Environmental Safety, 2010

Mussels coming from a mussel farm at Brown Bay (Beagle Channel) were transplanted to four sites i... more Mussels coming from a mussel farm at Brown Bay (Beagle Channel) were transplanted to four sites inside Ushuaia Bay for 2 and 4 weeks. The objective of this study was to assess the quality of coastal waters of Ushuaia Bay by measuring catalase activity, lipid peroxidation, total lipid content, bioaccumulation of heavy metals and condition index in transplanted mussel Mytilus edulis chilensis. Biomarkers except condition index showed significant differences among exposure times as well as among tissues. Digestive gland presented the highest catalase activity, malondialdehyde level and total lipid content. Digestive gland also was the main target tissue of accumulation of iron and copper, while gill accumulated the highest levels of zinc. A principal component analyzes with the whole set of data allowed to separate stations based on physicochemical conditions and biochemical responses of each studied area.