Amin Purfard - Academia.edu (original) (raw)

Papers by Amin Purfard

Asian Pacific Journal of Tropical Biomedicine, 2012

LWT - Food Science and Technology, 2014

ABSTRACT Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflo... more ABSTRACT Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflora essential oil (ZMO, 2, 4, 6 and 8% w/w of gelatin). Scanning electron microscopy observations indicate that ZMO droplets were well dispersed in the film matrix. Water solubility, water swelling, water uptake, water vapor permeability, tensile strength, elongation at break and Young's modulus for gelatin films were 27 ± 0.8%, 391 ± 11%, 135 ± 5%, 0.22 ± 0.014 g mm/m2 kPa h, 4.4 ± 0.4 MPa, 125 ± 7% and 8.8 ± 0.4 MPa, respectively. Incorporation of ZMO into gelatin films caused a significant decrease in swelling and water uptake and increase in solubility and water vapor permeability, a significant decrease in tensile strength, increase in elongation at break, decrease in Young's modulus of the films, dose-dependently. Gelatin/ZMO showed UV–visible light absorbance/transmission ranging from 280 to 480 nm with maximum absorbance at 420 nm. Gelatin films exhibited very low antioxidant activity while, gelatin/ZMO films exhibited excellent antioxidant properties. The gelatin/ZMO films also exhibited excellent antibacterial properties against both Gram-positive and Gram-negative bacteria. Our results suggested that the gelatin/ZMO films could be used as an active film due to its excellent antioxidant and antimicrobial properties for food packaging applications.

Journal of Food Science, 2013

The aim of this study was to determine the properties of gelatin films incorporated with thymol. ... more The aim of this study was to determine the properties of gelatin films incorporated with thymol. Gelatin films were prepared from gelatin solutions (10% w/v) containing thymol (1, 2, 4, and 8% w/w), glycerol (25% w/w) as plasticizer, and glutaraldehyde (2% w/w) as cross-linker. Cross-likened films showed higher tensile strength, higher elongation at break, lower Young's modulus, lower water solubility, lower swelling, lower water uptake, and lower water vapor permeability. Incorporation of thymol caused a significant decrease in tensile strength, increase in elongation at break, decrease in Young's modulus, increase in water solubility, decrease in swelling and water uptake, and increase in water vapor permeability slightly. The films incorporated with thymol exhibited excellent antioxidant and antibacterial properties. The antibacterial activity of the films containing thymol was greatest against Staphylucoccus aureus followed by Bacillus subtilis followed by Escherichia coli and then by Pseudomonas aeruginosa. Thus, gelatin films-containing thymol can be used as safe and effective source of natural antioxidant and antimicrobial agents with the purpose of evaluating their potential use as modern nano wound dressing. This study clearly demonstrates the potential of gelatin films incorporated with thymol as natural antioxidant and antimicrobial nano film. Such antimicrobial films exhibited excellent mechanical, physical, and water activities and could be used as antibacterial nano wound dressing against wounds burn pathogens.

Comparative Clinical Pathology, 2012

Hydatidosis in humans and animals is a substantial cause of deaths in many parts of the world. Su... more Hydatidosis in humans and animals is a substantial cause of deaths in many parts of the world. Surgery and the chemotherapeutic agents are the main treatments for hydatid disease; however, most of them are accompanied by adverse side effects. In this study, the scolicidal effect of essential oils from Zataria multiflora and Ferula assafoetida against Echinococcus granulosus protoscolices was investigated. Essential oils from Z. multiflora and F. assafoetida were prepared by hydrodistillation and analyzed by gas chromatography–mass spectrometry. Carvacrol (29.2 %), thymol (25.4 %), p-cymene (11.2 %), linalool (9.6 %), and γ-terpinene (8 %) were detected as the main components of the Z. multiflora, while the main components of F. assafoetida were E-1-propenyl-sec-butyl disulfide (62.7 %), β-ocimene (21.7 %) and β-pinene (5 %). Scolicidal activities of the essential oils against protoscolices for Z. multiflora and F. assafoetida were obtained at concentrations more than 17 and 60 μg/mL, respectively. Accordingly, it was concluded that both essential oils can selectively reduce protoscolices viability, and Z. multiflora, which contains phenolic monoterpenes, is more cytotoxic rather than F. assafoetida which contains disulfide compounds. Therefore, Z. multiflora and F. assafoetida could be recommended as a treatment against hydatid cyst.

Background: Development of biodegradable and biocompatible films based on protein polymer with st... more Background: Development of biodegradable and biocompatible films based on protein polymer with strong antioxidant and antibacterial activities has gradually obtained extensive concern in the world. In this study, the improvement of gelatin film properties incorporated with Ferula assa-foetida essential oil (FAO) as a potential antioxidant/antibacterial wound dressing film was investigated. Materials and Methods: Gelatin films were prepared from gelatin solutions (10% w/v) containing different concentration of FAO. The effect of FAO addition on water solubility, water swelling, water vapor permeability, mechanical behavior, light barrier properties as well as antioxidant and antibacterial activities of the films were examined. Results: Water solubility, water swelling and water vapor permeability for pure gelatin films were 29 ± 1.6%, 396 ± 8%, 0.23 ± 0.018 g.mm/m2.h, respectively. Incorporation of FAO into gelatin films caused a significant decrease in swelling and increase in solub...

Journal of Food Safety, 2012

The composition, reactive oxygen species, reactive nitrogen species, hydrogen peroxide and thioba... more The composition, reactive oxygen species, reactive nitrogen species, hydrogen peroxide and thiobarbituric acid reactive substances scavenging, antibacterial and antifungal activities of aqueous extract and essential oil from Zataria multiflora was examined. Carvacrol (29.489%), thymol (25.701%), p-cymene (11.247%), linalool (9.363%) and g-terpinene (8.054%) were detected as the main components of the oil. The inhibitory concentrations for total radical scavenging activities were between 1.92-3.15 and 2.46-4.58 mg/mL of aqueous extract and essential oil, respectively. The minimum inhibitory concentration for Salmonella typhi, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermi, Bacillus subtilis, Aspergillus niger and Candida albicans were 2.65 Ϯ 0.7, 2.72 Ϯ 0.8, 2.85 Ϯ 0.5, 3.02 Ϯ 0.8, 3.53 Ϯ 1, 3.65 Ϯ 0.9, 2.2 Ϯ 0.5 and 2.8 Ϯ 0.8 mg/mL of essential oil. However, aqueous extract cannot inhibit all of the bacteria and fungi tested. Thus, Z. multiflora essential oil has antioxidant and antimicrobial activity and can be used in the therapy of oxidative damage and in the therapy of infective diseases and can be used as a tea or additive in foods and traditional remedies for the treatment of infectious diseases. PRACTICAL APPLICATIONS Oxidative stress-related and infective diseases are one of the most common health problems in the worldwide population. A large portion of the world's population currently uses traditional medicines and herbal therapy, which involves the use of the aqueous extract of a plant in the form of an herbal tea. Essential oil derived from herbal medicines that have been known for their biological activities for many decades and should not be toxic to man and could replace chemical antioxidant, antibacterial and antifungal agents. This study clearly demonstrates the potential of essential oil from Zataria multiflora as antioxidant against reactive oxygen species, reactive nitrogen species, hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances and the potential of essential oil from Z. multiflora as antimicrobial against Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermi, Bacillus subtilis, Aspergillus niger and Candida albicans. The chemical composition of essential oil was identified. Thus, identification of such compounds also helps to discover of new antioxidant, antibacterial and antifungal agents.

Asian Pacific Journal of Tropical Biomedicine, 2012

LWT - Food Science and Technology, 2014

Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflora essent... more Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflora essential oil (ZMO, 2, 4, 6 and 8% w/w of gelatin). Scanning electron microscopy observations indicate that ZMO droplets were well dispersed in the film matrix. Water solubility, water swelling, water uptake, water vapor permeability, tensile strength, elongation at break and Young's modulus for gelatin films were 27 AE 0.8%, 391 AE 11%, 135 AE 5%, 0.22 AE 0.014 g mm/m 2 kPa h, 4.4 AE 0.4 MPa, 125 AE 7% and 8.8 AE 0.4 MPa, respectively. Incorporation of ZMO into gelatin films caused a significant decrease in swelling and water uptake and increase in solubility and water vapor permeability, a significant decrease in tensile strength, increase in elongation at break, decrease in Young's modulus of the films, dose-dependently. Gelatin/ ZMO showed UVevisible light absorbance/transmission ranging from 280 to 480 nm with maximum absorbance at 420 nm. Gelatin films exhibited very low antioxidant activity while, gelatin/ZMO films exhibited excellent antioxidant properties. The gelatin/ZMO films also exhibited excellent antibacterial properties against both Gram-positive and Gram-negative bacteria. Our results suggested that the gelatin/ZMO films could be used as an active film due to its excellent antioxidant and antimicrobial properties for food packaging applications.

Journal of Food Safety, 2013

Gelatin films were prepared from gelatin solutions (10% w/v) containing carvacrol (1%, 2%, 3%, 4%... more Gelatin films were prepared from gelatin solutions (10% w/v) containing carvacrol (1%, 2%, 3%, 4% and 5% w/w), glycerol (25% w/w) as plasticizer and glutaraldehyde (1% w/w) as cross-linker. The mechanical, water solubility, water swelling, water uptake, water vapor permeability, antioxidant and antibacterial properties of the films were obtained according to American Society for Testing and Materials. Gelatin films exhibited good tensile strength, elongation at break, water solubility, swelling, water uptake and water vapor permeability. Incorporation of carvacrol into the gelatin films caused a significant decrease in tensile strength, increase in elongation at break, increase in water solubility, decrease in swelling and water uptake, and increase in water vapor permeability of the films. Gelatin films exhibited low antioxidant activity while, gelatin films incorporated with carvacrol exhibited excellent antioxidant properties. The films incorporated with carvacrol also exhibited excellent antibacterial properties against both grampositive and gram-negative bacteria.

Journal of Food Science, 2013

The aim of this study was to determine the properties of gelatin films incorporated with thymol. ... more The aim of this study was to determine the properties of gelatin films incorporated with thymol. Gelatin films were prepared from gelatin solutions (10% w/v) containing thymol (1, 2, 4, and 8% w/w), glycerol (25% w/w) as plasticizer, and glutaraldehyde (2% w/w) as cross-linker. Cross-likened films showed higher tensile strength, higher elongation at break, lower Young's modulus, lower water solubility, lower swelling, lower water uptake, and lower water vapor permeability. Incorporation of thymol caused a significant decrease in tensile strength, increase in elongation at break, decrease in Young's modulus, increase in water solubility, decrease in swelling and water uptake, and increase in water vapor permeability slightly. The films incorporated with thymol exhibited excellent antioxidant and antibacterial properties. The antibacterial activity of the films containing thymol was greatest against Staphylucoccus aureus followed by Bacillus subtilis followed by Escherichia coli and then by Pseudomonas aeruginosa. Thus, gelatin films-containing thymol can be used as safe and effective source of natural antioxidant and antimicrobial agents with the purpose of evaluating their potential use as modern nano wound dressing. This study clearly demonstrates the potential of gelatin films incorporated with thymol as natural antioxidant and antimicrobial nano film. Such antimicrobial films exhibited excellent mechanical, physical, and water activities and could be used as antibacterial nano wound dressing against wounds burn pathogens.

Asian Pacific Journal of Tropical Biomedicine, 2012

LWT - Food Science and Technology, 2014

ABSTRACT Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflo... more ABSTRACT Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflora essential oil (ZMO, 2, 4, 6 and 8% w/w of gelatin). Scanning electron microscopy observations indicate that ZMO droplets were well dispersed in the film matrix. Water solubility, water swelling, water uptake, water vapor permeability, tensile strength, elongation at break and Young's modulus for gelatin films were 27 ± 0.8%, 391 ± 11%, 135 ± 5%, 0.22 ± 0.014 g mm/m2 kPa h, 4.4 ± 0.4 MPa, 125 ± 7% and 8.8 ± 0.4 MPa, respectively. Incorporation of ZMO into gelatin films caused a significant decrease in swelling and water uptake and increase in solubility and water vapor permeability, a significant decrease in tensile strength, increase in elongation at break, decrease in Young's modulus of the films, dose-dependently. Gelatin/ZMO showed UV–visible light absorbance/transmission ranging from 280 to 480 nm with maximum absorbance at 420 nm. Gelatin films exhibited very low antioxidant activity while, gelatin/ZMO films exhibited excellent antioxidant properties. The gelatin/ZMO films also exhibited excellent antibacterial properties against both Gram-positive and Gram-negative bacteria. Our results suggested that the gelatin/ZMO films could be used as an active film due to its excellent antioxidant and antimicrobial properties for food packaging applications.

Journal of Food Science, 2013

The aim of this study was to determine the properties of gelatin films incorporated with thymol. ... more The aim of this study was to determine the properties of gelatin films incorporated with thymol. Gelatin films were prepared from gelatin solutions (10% w/v) containing thymol (1, 2, 4, and 8% w/w), glycerol (25% w/w) as plasticizer, and glutaraldehyde (2% w/w) as cross-linker. Cross-likened films showed higher tensile strength, higher elongation at break, lower Young's modulus, lower water solubility, lower swelling, lower water uptake, and lower water vapor permeability. Incorporation of thymol caused a significant decrease in tensile strength, increase in elongation at break, decrease in Young's modulus, increase in water solubility, decrease in swelling and water uptake, and increase in water vapor permeability slightly. The films incorporated with thymol exhibited excellent antioxidant and antibacterial properties. The antibacterial activity of the films containing thymol was greatest against Staphylucoccus aureus followed by Bacillus subtilis followed by Escherichia coli and then by Pseudomonas aeruginosa. Thus, gelatin films-containing thymol can be used as safe and effective source of natural antioxidant and antimicrobial agents with the purpose of evaluating their potential use as modern nano wound dressing. This study clearly demonstrates the potential of gelatin films incorporated with thymol as natural antioxidant and antimicrobial nano film. Such antimicrobial films exhibited excellent mechanical, physical, and water activities and could be used as antibacterial nano wound dressing against wounds burn pathogens.

Comparative Clinical Pathology, 2012

Hydatidosis in humans and animals is a substantial cause of deaths in many parts of the world. Su... more Hydatidosis in humans and animals is a substantial cause of deaths in many parts of the world. Surgery and the chemotherapeutic agents are the main treatments for hydatid disease; however, most of them are accompanied by adverse side effects. In this study, the scolicidal effect of essential oils from Zataria multiflora and Ferula assafoetida against Echinococcus granulosus protoscolices was investigated. Essential oils from Z. multiflora and F. assafoetida were prepared by hydrodistillation and analyzed by gas chromatography–mass spectrometry. Carvacrol (29.2 %), thymol (25.4 %), p-cymene (11.2 %), linalool (9.6 %), and γ-terpinene (8 %) were detected as the main components of the Z. multiflora, while the main components of F. assafoetida were E-1-propenyl-sec-butyl disulfide (62.7 %), β-ocimene (21.7 %) and β-pinene (5 %). Scolicidal activities of the essential oils against protoscolices for Z. multiflora and F. assafoetida were obtained at concentrations more than 17 and 60 μg/mL, respectively. Accordingly, it was concluded that both essential oils can selectively reduce protoscolices viability, and Z. multiflora, which contains phenolic monoterpenes, is more cytotoxic rather than F. assafoetida which contains disulfide compounds. Therefore, Z. multiflora and F. assafoetida could be recommended as a treatment against hydatid cyst.

Background: Development of biodegradable and biocompatible films based on protein polymer with st... more Background: Development of biodegradable and biocompatible films based on protein polymer with strong antioxidant and antibacterial activities has gradually obtained extensive concern in the world. In this study, the improvement of gelatin film properties incorporated with Ferula assa-foetida essential oil (FAO) as a potential antioxidant/antibacterial wound dressing film was investigated. Materials and Methods: Gelatin films were prepared from gelatin solutions (10% w/v) containing different concentration of FAO. The effect of FAO addition on water solubility, water swelling, water vapor permeability, mechanical behavior, light barrier properties as well as antioxidant and antibacterial activities of the films were examined. Results: Water solubility, water swelling and water vapor permeability for pure gelatin films were 29 ± 1.6%, 396 ± 8%, 0.23 ± 0.018 g.mm/m2.h, respectively. Incorporation of FAO into gelatin films caused a significant decrease in swelling and increase in solub...

Journal of Food Safety, 2012

The composition, reactive oxygen species, reactive nitrogen species, hydrogen peroxide and thioba... more The composition, reactive oxygen species, reactive nitrogen species, hydrogen peroxide and thiobarbituric acid reactive substances scavenging, antibacterial and antifungal activities of aqueous extract and essential oil from Zataria multiflora was examined. Carvacrol (29.489%), thymol (25.701%), p-cymene (11.247%), linalool (9.363%) and g-terpinene (8.054%) were detected as the main components of the oil. The inhibitory concentrations for total radical scavenging activities were between 1.92-3.15 and 2.46-4.58 mg/mL of aqueous extract and essential oil, respectively. The minimum inhibitory concentration for Salmonella typhi, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermi, Bacillus subtilis, Aspergillus niger and Candida albicans were 2.65 Ϯ 0.7, 2.72 Ϯ 0.8, 2.85 Ϯ 0.5, 3.02 Ϯ 0.8, 3.53 Ϯ 1, 3.65 Ϯ 0.9, 2.2 Ϯ 0.5 and 2.8 Ϯ 0.8 mg/mL of essential oil. However, aqueous extract cannot inhibit all of the bacteria and fungi tested. Thus, Z. multiflora essential oil has antioxidant and antimicrobial activity and can be used in the therapy of oxidative damage and in the therapy of infective diseases and can be used as a tea or additive in foods and traditional remedies for the treatment of infectious diseases. PRACTICAL APPLICATIONS Oxidative stress-related and infective diseases are one of the most common health problems in the worldwide population. A large portion of the world's population currently uses traditional medicines and herbal therapy, which involves the use of the aqueous extract of a plant in the form of an herbal tea. Essential oil derived from herbal medicines that have been known for their biological activities for many decades and should not be toxic to man and could replace chemical antioxidant, antibacterial and antifungal agents. This study clearly demonstrates the potential of essential oil from Zataria multiflora as antioxidant against reactive oxygen species, reactive nitrogen species, hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances and the potential of essential oil from Z. multiflora as antimicrobial against Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermi, Bacillus subtilis, Aspergillus niger and Candida albicans. The chemical composition of essential oil was identified. Thus, identification of such compounds also helps to discover of new antioxidant, antibacterial and antifungal agents.

Asian Pacific Journal of Tropical Biomedicine, 2012

LWT - Food Science and Technology, 2014

Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflora essent... more Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflora essential oil (ZMO, 2, 4, 6 and 8% w/w of gelatin). Scanning electron microscopy observations indicate that ZMO droplets were well dispersed in the film matrix. Water solubility, water swelling, water uptake, water vapor permeability, tensile strength, elongation at break and Young's modulus for gelatin films were 27 AE 0.8%, 391 AE 11%, 135 AE 5%, 0.22 AE 0.014 g mm/m 2 kPa h, 4.4 AE 0.4 MPa, 125 AE 7% and 8.8 AE 0.4 MPa, respectively. Incorporation of ZMO into gelatin films caused a significant decrease in swelling and water uptake and increase in solubility and water vapor permeability, a significant decrease in tensile strength, increase in elongation at break, decrease in Young's modulus of the films, dose-dependently. Gelatin/ ZMO showed UVevisible light absorbance/transmission ranging from 280 to 480 nm with maximum absorbance at 420 nm. Gelatin films exhibited very low antioxidant activity while, gelatin/ZMO films exhibited excellent antioxidant properties. The gelatin/ZMO films also exhibited excellent antibacterial properties against both Gram-positive and Gram-negative bacteria. Our results suggested that the gelatin/ZMO films could be used as an active film due to its excellent antioxidant and antimicrobial properties for food packaging applications.

Journal of Food Safety, 2013

Gelatin films were prepared from gelatin solutions (10% w/v) containing carvacrol (1%, 2%, 3%, 4%... more Gelatin films were prepared from gelatin solutions (10% w/v) containing carvacrol (1%, 2%, 3%, 4% and 5% w/w), glycerol (25% w/w) as plasticizer and glutaraldehyde (1% w/w) as cross-linker. The mechanical, water solubility, water swelling, water uptake, water vapor permeability, antioxidant and antibacterial properties of the films were obtained according to American Society for Testing and Materials. Gelatin films exhibited good tensile strength, elongation at break, water solubility, swelling, water uptake and water vapor permeability. Incorporation of carvacrol into the gelatin films caused a significant decrease in tensile strength, increase in elongation at break, increase in water solubility, decrease in swelling and water uptake, and increase in water vapor permeability of the films. Gelatin films exhibited low antioxidant activity while, gelatin films incorporated with carvacrol exhibited excellent antioxidant properties. The films incorporated with carvacrol also exhibited excellent antibacterial properties against both grampositive and gram-negative bacteria.

Journal of Food Science, 2013

The aim of this study was to determine the properties of gelatin films incorporated with thymol. ... more The aim of this study was to determine the properties of gelatin films incorporated with thymol. Gelatin films were prepared from gelatin solutions (10% w/v) containing thymol (1, 2, 4, and 8% w/w), glycerol (25% w/w) as plasticizer, and glutaraldehyde (2% w/w) as cross-linker. Cross-likened films showed higher tensile strength, higher elongation at break, lower Young's modulus, lower water solubility, lower swelling, lower water uptake, and lower water vapor permeability. Incorporation of thymol caused a significant decrease in tensile strength, increase in elongation at break, decrease in Young's modulus, increase in water solubility, decrease in swelling and water uptake, and increase in water vapor permeability slightly. The films incorporated with thymol exhibited excellent antioxidant and antibacterial properties. The antibacterial activity of the films containing thymol was greatest against Staphylucoccus aureus followed by Bacillus subtilis followed by Escherichia coli and then by Pseudomonas aeruginosa. Thus, gelatin films-containing thymol can be used as safe and effective source of natural antioxidant and antimicrobial agents with the purpose of evaluating their potential use as modern nano wound dressing. This study clearly demonstrates the potential of gelatin films incorporated with thymol as natural antioxidant and antimicrobial nano film. Such antimicrobial films exhibited excellent mechanical, physical, and water activities and could be used as antibacterial nano wound dressing against wounds burn pathogens.