Amir Khaki - Academia.edu (original) (raw)
Papers by Amir Khaki
Crescent Journal of Medical and Biological Sciences, 2015
Objective: Nowadays, modern technologies are increasingly used in domestic industries, home appli... more Objective: Nowadays, modern technologies are increasingly used in domestic industries, home appliances, and cell phones. This has highlighted the necessity of protecting human beings from the impacts of electromagnetic fields (EMFs) as a new challenge. Therefore, the present study aimed to find out what impacts EMFs have on the structure of Langerhans Islets and the insulin secretion levels in rat. Materials and Methods: Fourteen 12-week-old male rats were selected randomly: 5 as a control group and 9 as an experimental group. The experimental group was exposed to an EMF produced by an electromagnetic device, with a frequency of 50 Hz and intensity of 3 mT 4 h a day for 6 weeks. At the end of the 6 weeks, blood and pancreas tissue samples were taken for enzyme linked immunosorbent assay (ELISA) test and preparation for microscopic studies. Results: Results showed that in EMF exposed group insulin level was decreased (P < 0.05), which was associated with decreased area and perimet...
The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructoseegg yo... more The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructoseegg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in this study. The semen samples were diluted by Tris-glucoseegg yolk. Diluted semen sample was divided into three parts. The first part was added with 0.9 mg mL-1 vitamin C, the second part was added with 1 mg mL-1 vitamin E and the third part was considered as a control without any addition. The diluted semen samples were cooled gradually and preserved at 5 ˚C for five days. Sperms in cooled diluted semen samples were examined for motility, vitality, abnormalities and acrosomal defects every 24 hr for five days. Results of the present study showed an increase in the viability of spermatozoa diluted in the Tris diluent containing vitamins C or E stored at 5 ˚C for 120 hr compared with the control group. There were significant (p < 0.05) effects of vitamins C and E addition to semen diluents on sperm motility as well as the sperm viability in different times of preservation at 5 ˚C. Significant (p < 0.05) higher sperm abnormalities and acrosomal defects values (37.6 ± 1.3% and 71.5 ± 1.1%, respectively) were found after 120 hr incubation in Tris free vitamin C (Control) at 5 ˚C compared with those of containing vitamin C (18.8 ± 1.8% and 52.8 ± 4.3%, respectively). From the results of the present study, it could be concluded, that the addition of antioxidants such as vitamins C and vitamin E to semen preservation media could improve longevity and quality of cooled sperm in Awassi ram semen.
African Journal of …, 2011
African Journal of Pharmacy and Pharmacology Vol. 5(7), pp. 903-907, July 2011 Available online h... more African Journal of Pharmacy and Pharmacology Vol. 5(7), pp. 903-907, July 2011 Available online http://www.academicjournals.org/ajpp ISSN 1996-0816 ©2011 Academic Journals ... Recovery of spermatogenesis by Allium cepa in ... Arash Khaki1, Laya Farzadi1, Shahin Ahmadi1*, ...
Journal of Medicinal Plants Research, 2010
... They may include weight loss, anemia, (Khalil-Manesh et al., 1994; Waldron, 1966) memory loss... more ... They may include weight loss, anemia, (Khalil-Manesh et al., 1994; Waldron, 1966) memory loss, (Hopkins, 1970) nephropathy, infertility, liver, testis and heart damages' (Patocka et al., 2003; Gurer-Orhan et al., 2004) etc. ... Khaki A, Heidari M, Ghaffari Novin M, Khaki AA (2008). ...
Journal of Information Technology & Software Engineering, 2019
“Network security” and “information security” are mostly used interchangeably. In fact, as the us... more “Network security” and “information security” are mostly used interchangeably. In fact, as the users and staffs do not know much about the security, intruders can easily enter a computer network reaching secret information. Due to the increasing requirement of users to computer networks, the present study aims to present the algorithms and methods to enhance the security of the networks. As a matter of fact, it is tried to increase the security of sending packages according to cryptography algorithms and decrease the package size by compression method. Therefore, traffic load in connection lines will decrease. Besides, authentication is done by bloom filter in this study. It is expected to guarantee the security of sending packages banning intruders to enter the system.
Background: Cyclo-oxygenase-2 5 T (COX-2) 5 T specific inhibitors were examined for predication o... more Background: Cyclo-oxygenase-2 5 T (COX-2) 5 T specific inhibitors were examined for predication or treatment of different tumors and it is indicated that COX-2 specific inhibitors play an important regulatory role in apoptosis of tumoral tissues. Therefore, the present study was designed in order to examine the preventive effects of a COX-2 specific inhibitor called celecoxib on 5 T 4-nitroquinoline 1-oxide (4NQO)-induced squamous cell carcinoma on rat5 T. Materials and Methods: In this experimental study, 30 Sprague Dawley rats (with the age of 3-3.5 months) were selected and divided into three groups. In order to induce lingual carcinoma, 4NQO powder was prepared 3 times a week for each cage. In this study, celecoxib power was mixed with a basic food (basal diet) in order to examine the systematic effect. Tongue samples were sent to laboratory for immunohistochemical (IHC) staining and histological examination4 T. Results: Based on morphological criteria and the ratio of apoptosis to cell proliferation, the prevalence of tongue precancerous lesions was reduced significantly by celecoxib. Conclusion: Celecoxib systematic has inhibitory effects on the 5 T 4-nitroquinoline 1-oxide (4NQO)-induced squamous cell carcinoma of tongue. The effect of celecoxib is probably via suppression of cell proliferation and induction of apoptosis4 T 5 T .4 T
Toxicology Letters - TOXICOL LETT, 2010
Middle East Fertility Society Journal, 2012
Abstract Cryopreservation of human oocytes and embryos or blastocyts is an important choice in as... more Abstract Cryopreservation of human oocytes and embryos or blastocyts is an important choice in assisted reproduction treatment that leads to an increased cumulative outcome while decreasing other attempts’ costs. It provides an opportunity for patients to have more than one attempt following an ovarian stimulation cycle, thereby decreasing the exposure of patients to exogenous gonadotrophins. Vitrification is a cryopreservation technique that leads to a glass-like solidification. Oocyte, zygote, embryo and blastocyst freezing by vitrification method for cryopreservation have been used for many years beside sperms preservation. Moreover, the use of vitrification technology for ovarian tissue cryopreservation to freeze eggs offers such an elderly women who sometime find more difficulty in conceiving or in maintaining pregnancy till full term because of old age compared to relatively younger women who might get better chances to get a healthy pregnancy. Furthermore, vitrification helps cancer patients who are looking to preserve their fertility later on after completing their treatment.
The Annals of Thoracic Surgery, 1998
The postoperative course was satisfactory. Chemotherapy with vincristine, doxorubicin, etoposide,... more The postoperative course was satisfactory. Chemotherapy with vincristine, doxorubicin, etoposide, prednisolone, and pentostatin was performed for 6 months after the operation. Since the completion of that chemotherapy, no further chemotherapy has been performed, and the patient was doing well without any sign of recurrence as of April 1998. Comment Surgical treatment of cardiac malignant lymphoma, both primary and metastatic, usually does not obtain satisfactory results [1, 2]. The occurrence of a tumor (whether malignant or benign) in the left ventricular outflow is rare. Malignant lymphoma is sometimes accompanied by acquired immunodeficiency syndrome or occurs after heart transplantation [3, 4]. Adult T cell leukemialymphoma is also sometimes accompanied by malignant lymphoma or lymphoma. The present patient was positive for anti-human T lymphotrophic virus-1 antibody, although symptomatic ATL had not developed, suggesting a relationship between ATL and the occurrence of malignant lymphoma [5]. Although the tumor was partially connected with the base of the left coronary cusp of the aortic valve, the border between the tumor and aortic valve was relatively clear. Because ATL is often accompanied by a hemorrhagic tendency, we avoided valve replacement in this patient. Although the results of chemotherapy for malignant lymphoma are improving, patients still sometimes die of infectious complications before complete remission. We experienced a patient with cardiac primary malignant lymphoma who died of pneumonia when the lymphoma was almost cured [6]. In the present case, the patient's young age, good response to chemotherapy, and the fact that ATL had not yet appeared even though the patient was positive for antihuman T lymphotropic virus-1 antibody may be the reasons for the very long survival period.
… of Hospitality Management, 2004
Veterinary Research Forum, 2012
To investigate uterine and serum Ca++ and Mg++ variations during the estrous cycle in the bovine,... more To investigate uterine and serum Ca++ and Mg++ variations during the estrous cycle in the bovine, 66 genital tracts and blood samples were collected from Urmia abattoir, Urmia, Iran. The phase of the estrous cycle was determined by examination of the structures present on ovaries and uterine tonicity. Of the collected samples, 17 were pro-estrus, 12 estrus, 14 metestrus and 23 diestrus. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. The mean ± SEM concentration of serum Ca++ in pro-estrus, estrus, metestrus and diestrus was 5.77 ± 0.69, 8.87 ± 1.83, 10.95 ± 1.52, 11.09 ± 1.08 mg dL-1, and the mean concentration of uterine fluid Ca++ was 4.40 ± 0.72, 3.15 ± 0.67, 5.89 ± 0.88, 8.63 ± 0.97 mg dL-1, respectively. The mean concentration of serum Mg++ in pro-estrus, estrus, metestrus and diestrus was 3.53 ± 0.30, 4.20 ± 0.52, 3.49 ± 0.38, 3.39 ± 0.29 mg dL-1, and mean concentration of uterine fluid Mg++ was 5.27 ± 0.42, 4.92 ± 0.60, 5.56 ± 0.30, 5...
BackgroundOne interesting topic in reproductive biology is to explore the correlations between mi... more BackgroundOne interesting topic in reproductive biology is to explore the correlations between minerals and male fertility. The aim of the present study was to investigate the relationships between Calcium (Ca), Magnesium (Mg), Zinc (Zn), Copper (Cu), and Iron (Fe) and their associations with semen quality in bulls.MethodsEjaculates were collected from Simmental bulls. Semen quality parameters including volume, concentration, motility, sperm viability and morphology were assessed. One step dilution method was utilized for semen freezing. Progressive motility of frozen-thawed semen (PMFT) was regarded as the main criterion for dividing data into three groups: group 1 (˂ 40% motility), group 2 (40% -50% motility), and group 3 (> 50% motility). Serum mineral concentrations were determined by atomic absorption. The Serum Fe was significantly lower in “> 50%” group compared with other groups.ResultsSemen volume was negatively correlated with PMFT (r = -0.42, p ≤ 0.05 and serum Fe (...
Veterinary Research Forum, 2021
This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), cat... more This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in the first and second ejaculations and their relationships with semen quality parameters in Fleckvieh bulls. Repetitive ejaculates were separately collected, analyzed, and frozen from the sires. Progressive motility of frozen-thawed semen (PMFT) was considered the main factor for more data classification into three following groups: <40.00%, 40.00 - 50.00%, and >50.00%. Seminal plasma trace elements and enzymes were determined using atomic absorption spectrometry and ELISA, respectively. The results revealed significant differences between the first and second ejaculations. Semen concentration, SOD, GPx, and Fe were different in ejaculations. Although PMFT groups in different ejaculations did not show significant differences, there was significant alteration between different PMFT groups and first an...
Veterinary Research Forum, 2012
To investigate uterine and serum copper (Cu) and zinc (Zn) concentrations variation during the bo... more To investigate uterine and serum copper (Cu) and zinc (Zn) concentrations variation during the bovine estrus cycle , 232 blood and genital tract samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by the examination of the ovaries and the uterine tonicity. Of the 46 samples selected for use in the study, 13 were pro-estrus, 10 estrus, 8 metestrus, and 15 diestrus. The uterus was incised and uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. The total mean (± SEM) Cu concentrations in serum and uterine fluid samples, determined by spectrophotometry, were 66.1± 6.5 and 171.3 ± 33.2µg dL-1 respectively, which were significantly different, while total mean serum and uterine fluid Zn concentrations were 91.9 ± 5.4 and 291.6 ± 23.4 µg dL-1, which also showed a significant difference. The mean serum Cu values in different phases of the estrous cycle were not significantly different, while uterine fluid Cu conten...
Veterinary Research Forum, 2014
To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estr... more To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, 15 estrous, 16 met-estrous and 22 diestrous. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean ± SEM total serum and uterine fluid Ca in cyclic buffaloes were 8.68 ± 0.28 mg dL-1 and 8.10 ± 0.2 mg dL-1 vs. 6.76 ± 0.65 mg dL-1 and 7.90 ± 0.15 mg dL-1 in pre-pubertal calves, respectively. Blood serum Mg was not different in cyclic and pre-pubertal animals but the uterine fluid Mg in cyclic cows was higher than those in pre-pubertal calves. Serum Ca in pro-estrus and estrus were higher than those in other stag...
To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cy... more To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia, Iran. The phase of the estrous cycle was determined by examining ovarian structures; 18, 15, 16 and 22 were pro-estrous, estrous, met-estrous and diestrous, respectively. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean (± SEM) total serum (77.10 ± 1.50 µg dL-1) and uterine fluid (296.40 ± 9.40 μg dL-1) Cu in cyclic cows was higher than the values of 54.00 ± 1.10 μg dL-1 and 133.40 ± 5.70 μg dL-1 in pre-pubertal calves, respectively. Blood serum (114.60 ± 3.20 μg dL-1) and the uterine fluid (349.90 ± 8.90 μg dL-1) Zn content in cyclic cows were also higher than those (98.80 ± 1.50 μg dL-1 and 246.6 ± 4.50 μg dL-1 res...
Background The objective of the study was to investigate the effects of in vitro zinc sulphate ad... more Background The objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and DNA stability) after cryopreservation. Materials and Methods In this Prospective longitudinal laboratory study, semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions from Iran, Urmia during summer and autumn 2011, 25 samples were used in each treatment. Sperm progressive motility, viability and abnormal morphology were measured before and at 0.5 (T0), 1(T1) and 2(T2) hours after diluting semen(1:10 v/v) in Tris-citric acid based extender (without egg yolk and glycerol) at 37˚C containing none (control group), 0.072, 0.144, 0.288, 0.576 and 1.152 mg/L zinc sulphate to investigate dose and time effects. Next, a Tris-citric acid-egg yolk-glycerol extender (20% egg yolk and 7% glycerol) containing the same amount of zinc sulphate was prepared, diluted seme...
Crescent Journal of Medical and Biological Sciences, 2015
Objective: Nowadays, modern technologies are increasingly used in domestic industries, home appli... more Objective: Nowadays, modern technologies are increasingly used in domestic industries, home appliances, and cell phones. This has highlighted the necessity of protecting human beings from the impacts of electromagnetic fields (EMFs) as a new challenge. Therefore, the present study aimed to find out what impacts EMFs have on the structure of Langerhans Islets and the insulin secretion levels in rat. Materials and Methods: Fourteen 12-week-old male rats were selected randomly: 5 as a control group and 9 as an experimental group. The experimental group was exposed to an EMF produced by an electromagnetic device, with a frequency of 50 Hz and intensity of 3 mT 4 h a day for 6 weeks. At the end of the 6 weeks, blood and pancreas tissue samples were taken for enzyme linked immunosorbent assay (ELISA) test and preparation for microscopic studies. Results: Results showed that in EMF exposed group insulin level was decreased (P < 0.05), which was associated with decreased area and perimet...
The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructoseegg yo... more The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructoseegg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in this study. The semen samples were diluted by Tris-glucoseegg yolk. Diluted semen sample was divided into three parts. The first part was added with 0.9 mg mL-1 vitamin C, the second part was added with 1 mg mL-1 vitamin E and the third part was considered as a control without any addition. The diluted semen samples were cooled gradually and preserved at 5 ˚C for five days. Sperms in cooled diluted semen samples were examined for motility, vitality, abnormalities and acrosomal defects every 24 hr for five days. Results of the present study showed an increase in the viability of spermatozoa diluted in the Tris diluent containing vitamins C or E stored at 5 ˚C for 120 hr compared with the control group. There were significant (p < 0.05) effects of vitamins C and E addition to semen diluents on sperm motility as well as the sperm viability in different times of preservation at 5 ˚C. Significant (p < 0.05) higher sperm abnormalities and acrosomal defects values (37.6 ± 1.3% and 71.5 ± 1.1%, respectively) were found after 120 hr incubation in Tris free vitamin C (Control) at 5 ˚C compared with those of containing vitamin C (18.8 ± 1.8% and 52.8 ± 4.3%, respectively). From the results of the present study, it could be concluded, that the addition of antioxidants such as vitamins C and vitamin E to semen preservation media could improve longevity and quality of cooled sperm in Awassi ram semen.
African Journal of …, 2011
African Journal of Pharmacy and Pharmacology Vol. 5(7), pp. 903-907, July 2011 Available online h... more African Journal of Pharmacy and Pharmacology Vol. 5(7), pp. 903-907, July 2011 Available online http://www.academicjournals.org/ajpp ISSN 1996-0816 ©2011 Academic Journals ... Recovery of spermatogenesis by Allium cepa in ... Arash Khaki1, Laya Farzadi1, Shahin Ahmadi1*, ...
Journal of Medicinal Plants Research, 2010
... They may include weight loss, anemia, (Khalil-Manesh et al., 1994; Waldron, 1966) memory loss... more ... They may include weight loss, anemia, (Khalil-Manesh et al., 1994; Waldron, 1966) memory loss, (Hopkins, 1970) nephropathy, infertility, liver, testis and heart damages' (Patocka et al., 2003; Gurer-Orhan et al., 2004) etc. ... Khaki A, Heidari M, Ghaffari Novin M, Khaki AA (2008). ...
Journal of Information Technology & Software Engineering, 2019
“Network security” and “information security” are mostly used interchangeably. In fact, as the us... more “Network security” and “information security” are mostly used interchangeably. In fact, as the users and staffs do not know much about the security, intruders can easily enter a computer network reaching secret information. Due to the increasing requirement of users to computer networks, the present study aims to present the algorithms and methods to enhance the security of the networks. As a matter of fact, it is tried to increase the security of sending packages according to cryptography algorithms and decrease the package size by compression method. Therefore, traffic load in connection lines will decrease. Besides, authentication is done by bloom filter in this study. It is expected to guarantee the security of sending packages banning intruders to enter the system.
Background: Cyclo-oxygenase-2 5 T (COX-2) 5 T specific inhibitors were examined for predication o... more Background: Cyclo-oxygenase-2 5 T (COX-2) 5 T specific inhibitors were examined for predication or treatment of different tumors and it is indicated that COX-2 specific inhibitors play an important regulatory role in apoptosis of tumoral tissues. Therefore, the present study was designed in order to examine the preventive effects of a COX-2 specific inhibitor called celecoxib on 5 T 4-nitroquinoline 1-oxide (4NQO)-induced squamous cell carcinoma on rat5 T. Materials and Methods: In this experimental study, 30 Sprague Dawley rats (with the age of 3-3.5 months) were selected and divided into three groups. In order to induce lingual carcinoma, 4NQO powder was prepared 3 times a week for each cage. In this study, celecoxib power was mixed with a basic food (basal diet) in order to examine the systematic effect. Tongue samples were sent to laboratory for immunohistochemical (IHC) staining and histological examination4 T. Results: Based on morphological criteria and the ratio of apoptosis to cell proliferation, the prevalence of tongue precancerous lesions was reduced significantly by celecoxib. Conclusion: Celecoxib systematic has inhibitory effects on the 5 T 4-nitroquinoline 1-oxide (4NQO)-induced squamous cell carcinoma of tongue. The effect of celecoxib is probably via suppression of cell proliferation and induction of apoptosis4 T 5 T .4 T
Toxicology Letters - TOXICOL LETT, 2010
Middle East Fertility Society Journal, 2012
Abstract Cryopreservation of human oocytes and embryos or blastocyts is an important choice in as... more Abstract Cryopreservation of human oocytes and embryos or blastocyts is an important choice in assisted reproduction treatment that leads to an increased cumulative outcome while decreasing other attempts’ costs. It provides an opportunity for patients to have more than one attempt following an ovarian stimulation cycle, thereby decreasing the exposure of patients to exogenous gonadotrophins. Vitrification is a cryopreservation technique that leads to a glass-like solidification. Oocyte, zygote, embryo and blastocyst freezing by vitrification method for cryopreservation have been used for many years beside sperms preservation. Moreover, the use of vitrification technology for ovarian tissue cryopreservation to freeze eggs offers such an elderly women who sometime find more difficulty in conceiving or in maintaining pregnancy till full term because of old age compared to relatively younger women who might get better chances to get a healthy pregnancy. Furthermore, vitrification helps cancer patients who are looking to preserve their fertility later on after completing their treatment.
The Annals of Thoracic Surgery, 1998
The postoperative course was satisfactory. Chemotherapy with vincristine, doxorubicin, etoposide,... more The postoperative course was satisfactory. Chemotherapy with vincristine, doxorubicin, etoposide, prednisolone, and pentostatin was performed for 6 months after the operation. Since the completion of that chemotherapy, no further chemotherapy has been performed, and the patient was doing well without any sign of recurrence as of April 1998. Comment Surgical treatment of cardiac malignant lymphoma, both primary and metastatic, usually does not obtain satisfactory results [1, 2]. The occurrence of a tumor (whether malignant or benign) in the left ventricular outflow is rare. Malignant lymphoma is sometimes accompanied by acquired immunodeficiency syndrome or occurs after heart transplantation [3, 4]. Adult T cell leukemialymphoma is also sometimes accompanied by malignant lymphoma or lymphoma. The present patient was positive for anti-human T lymphotrophic virus-1 antibody, although symptomatic ATL had not developed, suggesting a relationship between ATL and the occurrence of malignant lymphoma [5]. Although the tumor was partially connected with the base of the left coronary cusp of the aortic valve, the border between the tumor and aortic valve was relatively clear. Because ATL is often accompanied by a hemorrhagic tendency, we avoided valve replacement in this patient. Although the results of chemotherapy for malignant lymphoma are improving, patients still sometimes die of infectious complications before complete remission. We experienced a patient with cardiac primary malignant lymphoma who died of pneumonia when the lymphoma was almost cured [6]. In the present case, the patient's young age, good response to chemotherapy, and the fact that ATL had not yet appeared even though the patient was positive for antihuman T lymphotropic virus-1 antibody may be the reasons for the very long survival period.
… of Hospitality Management, 2004
Veterinary Research Forum, 2012
To investigate uterine and serum Ca++ and Mg++ variations during the estrous cycle in the bovine,... more To investigate uterine and serum Ca++ and Mg++ variations during the estrous cycle in the bovine, 66 genital tracts and blood samples were collected from Urmia abattoir, Urmia, Iran. The phase of the estrous cycle was determined by examination of the structures present on ovaries and uterine tonicity. Of the collected samples, 17 were pro-estrus, 12 estrus, 14 metestrus and 23 diestrus. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. The mean ± SEM concentration of serum Ca++ in pro-estrus, estrus, metestrus and diestrus was 5.77 ± 0.69, 8.87 ± 1.83, 10.95 ± 1.52, 11.09 ± 1.08 mg dL-1, and the mean concentration of uterine fluid Ca++ was 4.40 ± 0.72, 3.15 ± 0.67, 5.89 ± 0.88, 8.63 ± 0.97 mg dL-1, respectively. The mean concentration of serum Mg++ in pro-estrus, estrus, metestrus and diestrus was 3.53 ± 0.30, 4.20 ± 0.52, 3.49 ± 0.38, 3.39 ± 0.29 mg dL-1, and mean concentration of uterine fluid Mg++ was 5.27 ± 0.42, 4.92 ± 0.60, 5.56 ± 0.30, 5...
BackgroundOne interesting topic in reproductive biology is to explore the correlations between mi... more BackgroundOne interesting topic in reproductive biology is to explore the correlations between minerals and male fertility. The aim of the present study was to investigate the relationships between Calcium (Ca), Magnesium (Mg), Zinc (Zn), Copper (Cu), and Iron (Fe) and their associations with semen quality in bulls.MethodsEjaculates were collected from Simmental bulls. Semen quality parameters including volume, concentration, motility, sperm viability and morphology were assessed. One step dilution method was utilized for semen freezing. Progressive motility of frozen-thawed semen (PMFT) was regarded as the main criterion for dividing data into three groups: group 1 (˂ 40% motility), group 2 (40% -50% motility), and group 3 (> 50% motility). Serum mineral concentrations were determined by atomic absorption. The Serum Fe was significantly lower in “> 50%” group compared with other groups.ResultsSemen volume was negatively correlated with PMFT (r = -0.42, p ≤ 0.05 and serum Fe (...
Veterinary Research Forum, 2021
This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), cat... more This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in the first and second ejaculations and their relationships with semen quality parameters in Fleckvieh bulls. Repetitive ejaculates were separately collected, analyzed, and frozen from the sires. Progressive motility of frozen-thawed semen (PMFT) was considered the main factor for more data classification into three following groups: <40.00%, 40.00 - 50.00%, and >50.00%. Seminal plasma trace elements and enzymes were determined using atomic absorption spectrometry and ELISA, respectively. The results revealed significant differences between the first and second ejaculations. Semen concentration, SOD, GPx, and Fe were different in ejaculations. Although PMFT groups in different ejaculations did not show significant differences, there was significant alteration between different PMFT groups and first an...
Veterinary Research Forum, 2012
To investigate uterine and serum copper (Cu) and zinc (Zn) concentrations variation during the bo... more To investigate uterine and serum copper (Cu) and zinc (Zn) concentrations variation during the bovine estrus cycle , 232 blood and genital tract samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by the examination of the ovaries and the uterine tonicity. Of the 46 samples selected for use in the study, 13 were pro-estrus, 10 estrus, 8 metestrus, and 15 diestrus. The uterus was incised and uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. The total mean (± SEM) Cu concentrations in serum and uterine fluid samples, determined by spectrophotometry, were 66.1± 6.5 and 171.3 ± 33.2µg dL-1 respectively, which were significantly different, while total mean serum and uterine fluid Zn concentrations were 91.9 ± 5.4 and 291.6 ± 23.4 µg dL-1, which also showed a significant difference. The mean serum Cu values in different phases of the estrous cycle were not significantly different, while uterine fluid Cu conten...
Veterinary Research Forum, 2014
To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estr... more To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, 15 estrous, 16 met-estrous and 22 diestrous. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean ± SEM total serum and uterine fluid Ca in cyclic buffaloes were 8.68 ± 0.28 mg dL-1 and 8.10 ± 0.2 mg dL-1 vs. 6.76 ± 0.65 mg dL-1 and 7.90 ± 0.15 mg dL-1 in pre-pubertal calves, respectively. Blood serum Mg was not different in cyclic and pre-pubertal animals but the uterine fluid Mg in cyclic cows was higher than those in pre-pubertal calves. Serum Ca in pro-estrus and estrus were higher than those in other stag...
To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cy... more To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia, Iran. The phase of the estrous cycle was determined by examining ovarian structures; 18, 15, 16 and 22 were pro-estrous, estrous, met-estrous and diestrous, respectively. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean (± SEM) total serum (77.10 ± 1.50 µg dL-1) and uterine fluid (296.40 ± 9.40 μg dL-1) Cu in cyclic cows was higher than the values of 54.00 ± 1.10 μg dL-1 and 133.40 ± 5.70 μg dL-1 in pre-pubertal calves, respectively. Blood serum (114.60 ± 3.20 μg dL-1) and the uterine fluid (349.90 ± 8.90 μg dL-1) Zn content in cyclic cows were also higher than those (98.80 ± 1.50 μg dL-1 and 246.6 ± 4.50 μg dL-1 res...
Background The objective of the study was to investigate the effects of in vitro zinc sulphate ad... more Background The objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and DNA stability) after cryopreservation. Materials and Methods In this Prospective longitudinal laboratory study, semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions from Iran, Urmia during summer and autumn 2011, 25 samples were used in each treatment. Sperm progressive motility, viability and abnormal morphology were measured before and at 0.5 (T0), 1(T1) and 2(T2) hours after diluting semen(1:10 v/v) in Tris-citric acid based extender (without egg yolk and glycerol) at 37˚C containing none (control group), 0.072, 0.144, 0.288, 0.576 and 1.152 mg/L zinc sulphate to investigate dose and time effects. Next, a Tris-citric acid-egg yolk-glycerol extender (20% egg yolk and 7% glycerol) containing the same amount of zinc sulphate was prepared, diluted seme...