Amitava Ghosh - Academia.edu (original) (raw)

Papers by Amitava Ghosh

Indo American Journal of Pharmaceutical Research, 2016

An experimental study with the objective to evaluate the acute toxicity study and anti-ulcerative... more An experimental study with the objective to evaluate the acute toxicity study and anti-ulcerative property of the polyherbal formulation named-ASC01 (formulated in the same institute) was performed as per OECD guidelines 423. The polyherbal ayurvedic formulation (PHAF) is a scientifically developed combination of herbs that contains herbal ingredients like Yashtimadhu, Shatavari which have reported antiulcer activity. Firstly an acute toxicity study was formulated (as per OECD guidelines 423) the animals were observed for signs of convulsions, tremors, circling, depression, excitement and mortality. No abnormal sign of symptoms were observed in any of the animal fed with the PHAF at the dose upto 5000 mg/kg body weight. No mortality was observed in any of the animals indicating its safety. The anti-ulcer property of the polyherbal formulation was evaluated on wistar albino rats grouped into 4 groups (n=6). The findings showed that the said polyherbal test extract (ASC01) helped in reversing duodenal and peptic ulcer in murine models. Please cite this article in press as Raja Chakraverty et al. Evaluation of the safety and anti-ulcerative potential of a polyherbal ayurvedic formulation (ASC01): an exploratory study. Indo American Journal of Pharmaceutical Research.2016:6(03).

Snakebite is a major health hazard that leads to high mortality rate, especially in India. The pr... more Snakebite is a major health hazard that leads to high mortality rate, especially in India. The present review article focuses on the point of view of different in–vitro neutralization assays that serve as an index for assessing the status of therapy. For this purpose bibliographic and scientific literature articles in indexed journal, databases were comprehensively researched, and contemporary articles were studied from different abstracting and indexing systems like PubMed using relevant keywords. It was found that some of the in-vitro assays like Phospholipase A2, Hemorrhagic activity assay, agglutination assay for potency assessment, general proteolytic activity assay, snake venom metalloprotease activity (SVMP), hemolytic activity, neutralization of serum inhibitory activity assay, neutralization of fibrinolytic activity, inhibition of venom cardiotoxic activity assay and brine shrimp lethality test are prevalent in use. Performing these in–vitro tests is essential for the devel...

The present study is aimed at assessment of the innate pharmacological potential of leaves of Ery... more The present study is aimed at assessment of the innate pharmacological potential of leaves of Eryngium foetidum. The study objective includes the evaluation of the different hydro-alcoholic concentrations of Eryngium foetidum extracted and to find correlates with its yield value and the biological effect in animal models of hepatoprotectivity. The plant was collected from a rural garden of Assam. The plant was subsequently authenticated at the Botanical Survey of India, Shibpur, Howrah and a specimen number was accorded a reference number and retained for future reference. The extraction process was carried out by maceration and the hydro-ethanol extract was prepared in different ratios of 30:70, 50:50 and 70: 30 respectively. The yield of the three different extracts was compared and the acute toxicity study was performed as per OECD guidelines 423 after obtaining prior permission from the Institutional animal ethics committee (IAEC). The study, however, showed no mortality or unto...

The purpose of this present work was to evaluate the antinociceptive efficacy of a combination of... more The purpose of this present work was to evaluate the antinociceptive efficacy of a combination of an opioid and a non-opioid analgesic in experimental models of nociceptive pain in swiss albino mice using established pharmacological models of pain. As of date, no single analgesic drug is considered perfect in the treatment of all types of pain. Each analgesic agent possesses some distinct advantages and disadvantages with respect to the others. Hence, the clinical outcomes might be improved in certain conditions with the use of a combination of two analgesics, rather than relying on a single one. A combination is most effective when the individual agents act through different mechanisms of analgesia and when they act synergistically. By activating multiple pain-inhibitory pathways, combination analgesics can provide more effective pain relief for a wider spectrum of pain, help in dose reduction and may also reduce adverse drug reactions. This work highlights the therapeutic potentia...

ISRN Pharmaceutics, 2013

Delivery of drugs into eyes using conventional drug delivery systems, such as solutions, is a con... more Delivery of drugs into eyes using conventional drug delivery systems, such as solutions, is a considerable challenge to the treatment of ocular diseases. Drug loss from the ocular surface by lachrymal fluid secretion, lachrymal fluid-eye barriers, and blood-ocular barriers are main obstacles. A number of ophthalmic drug delivery carriers have been made to improve the bioavailability and to prolong the residence time of drugs applied topically onto the eye. The potential use of microemulsions as an ocular drug delivery carrier offers several favorable pharmaceutical and biopharmaceutical properties such as their excellent thermodynamic stability, phase transition to liquid-crystal state, very low surface tension, and small droplet size, which may result in improved ocular drug retention, extended duration of action, high ocular absorption, and permeation of loaded drugs. Further, both lipophilic and hydrophilic characteristics are present in microemulsions, so that the loaded drugs c...

ISRN pharmaceutics, 2012

The aim of the present work was designed to develop a model-sustained release matrix tablet formu... more The aim of the present work was designed to develop a model-sustained release matrix tablet formulation for Metformin hydrochloride using wet granulation technique. In the present study the formulation design was employed to statistically optimize different parameters of Metformin hydrochloride tablets at different drug-to-polymer ratios employing polymers Hydroxypropyl methylcellulose of two grades K4M and K100M as two independent variables whereas the dependent variables studied were X(60), X(120), T(50), T(90), n, and b values obtained from dissolution kinetics data. The in vitro drug release studies were carried out at simulated intestinal fluids, and the release showed a non-Fickian anomalous transport mechanism. The drug release was found to reveal zero order kinetics. The granules and the tablets were tested for their normal physical, morphological, and analytical parameters and were found to be within the satisfactory levels. There were no significant drug-polymer interactio...

Industrial Crops and Products, 2013

ABSTRACT

Colloids and Surfaces B: Biointerfaces, 1995

Acta Pharmaceutica, Jun 15, 2012

A b s t r a c t A r t i c l e I n f o The present study is aimed at assessment of the innate phar... more A b s t r a c t A r t i c l e I n f o The present study is aimed at assessment of the innate pharmacological potential of leaves of Eryngium foetidum. The study objective includes the evaluation of the different hydro-alcoholic concentrations of Eryngium foetidum extracted and to find correlates with its yield value and the biological effect in animal models of hepatoprotectivity. The plant was collected from a rural garden of Assam. The plant was subsequently authenticated at the Botanical Survey of India, Shibpur, Howrah and a specimen number was accorded a reference number and retained for future reference. The extraction process was carried out by maceration and the hydro-ethanol extract was prepared in different ratios of 30:70, 50:50 and 70: 30 respectively. The yield of the three different extracts was compared and the acute toxicity study was performed as per OECD guidelines 423 after obtaining prior permission from the Institutional animal ethics committee (IAEC). The study, however, showed no mortality or untoward behavioural morbidities in the experimental animals post the oral acute toxicity study at doses up to 2000 mg/ kg body weight. Subsequently the efficacy study of the drug was performed using 18 Swiss albino mice (n=3) as a prospective pilot interventional efficacy study to evaluate the beneficial effect of the drug in hepatotoxicity induced by Carbon tetrachloride (CCl 4) in murine models. The experimental groups were: negative control, disease control, standard (silymarin treated) and hydro-alcoholic extract groups. The findings were statistically analyzed using two way-ANOVA at CI of 95% with p<0.05 from the efficacy study shows promise in putative role of this indigenous plant in the amelioration of hepatotoxicity and poses no signs of morbidity or mortality upto a dose of 2000 mg/kg body weight with the drug. Follow up studies with larger sample size are however, warranted to corroborate findings to definitely arrive at a meaningful conclusion regarding the standing and the hepatoprotective potential of this indigenous drug.

Antimicrobial Agents and Chemotherapy, Nov 1, 1996

Journal of the American Society For Mass Spectrometry, Sep 7, 2012