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Amna Mustafa

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Papers by Amna Mustafa

Research paper thumbnail of In vitro total phenolics, total flavonoids, antioxidant and antibacterial activities of selected medicinal plants using different solvent systems

BMC Chemistry

Recently, an interest has surged in utilizing indigenous medicinal plants to treat infectious ill... more Recently, an interest has surged in utilizing indigenous medicinal plants to treat infectious illnesses and extract bioactive substances, highlighting the need to analyze medicinal plants for phytochemicals and bioactivities. The present study was aimed to evaluate the impact of different solvent systems (aqueous, ethanol, and methanol) used for extraction on total phenolics, total flavonoids, antioxidant, and antibacterial activity of three medicinal plants of Azad Kashmir (Achillea millefolium,Bergenia ciliata,andAloe vera). High phenolic content was found in methanol extracts ofB. ciliata(27.48 ± 0.58 mg GAE/g dry weight),A. vera(25.61 ± 0.33 mg GAE/g dry weight), andA. millefolium(24.25 ± 0.67 mg GAE/g dry weight). High flavonoid content was obtained in the ethanol extract ofA. millefolium(27.13 ± 0.64 mg QE/g dry weight), methanol extract ofB. ciliata(17.44 ± 0.44 ± 0.44 mg QE/g dry weight), and the methanol extract ofA. vera(14.68 ± 0.67 mg QE/g dry weight). Strong 2,2-dipheny...

Research paper thumbnail of 15. Phytochemical evaluation and assessment of genetic diversity amongst Artemisia species collected from foothills of Himalayas

Artemisia is a significant genus in family Asteraceae and has large number of taxa having economi... more Artemisia is a significant genus in family Asteraceae and has large number of taxa having economic and ecological importance. The aim of the study was to analyze highly medicinal products as well, antioxidant activity and genetic variability in three species of Artemisia from various sites of district Poonch. For the evaluation of phytochemicals and antioxidants, different volumes of extracts and solvents were used from leaves. The species were analyzed for genetic diversity within and between various Artemisia species. For molecular investigation, random amplification of polymorphic DNA (RAPD) fragment was used to determine genetic variations between and within species. From of ten primers only six primers gave amplified products. By using all six primers, total 331 bands were amplified from which 30 were monomorphic, 301 were polymorphic and percentage of polymorphism was 91% in Artemisia japonica, 321 bands were amplified in Artemisia roxberghiana from which 21 were monomorphic, 3...

Research paper thumbnail of In vitro total phenolics, total flavonoids, antioxidant and antibacterial activities of selected medicinal plants using different solvent systems

BMC Chemistry

Recently, an interest has surged in utilizing indigenous medicinal plants to treat infectious ill... more Recently, an interest has surged in utilizing indigenous medicinal plants to treat infectious illnesses and extract bioactive substances, highlighting the need to analyze medicinal plants for phytochemicals and bioactivities. The present study was aimed to evaluate the impact of different solvent systems (aqueous, ethanol, and methanol) used for extraction on total phenolics, total flavonoids, antioxidant, and antibacterial activity of three medicinal plants of Azad Kashmir (Achillea millefolium,Bergenia ciliata,andAloe vera). High phenolic content was found in methanol extracts ofB. ciliata(27.48 ± 0.58 mg GAE/g dry weight),A. vera(25.61 ± 0.33 mg GAE/g dry weight), andA. millefolium(24.25 ± 0.67 mg GAE/g dry weight). High flavonoid content was obtained in the ethanol extract ofA. millefolium(27.13 ± 0.64 mg QE/g dry weight), methanol extract ofB. ciliata(17.44 ± 0.44 ± 0.44 mg QE/g dry weight), and the methanol extract ofA. vera(14.68 ± 0.67 mg QE/g dry weight). Strong 2,2-dipheny...

Research paper thumbnail of 15. Phytochemical evaluation and assessment of genetic diversity amongst Artemisia species collected from foothills of Himalayas

Artemisia is a significant genus in family Asteraceae and has large number of taxa having economi... more Artemisia is a significant genus in family Asteraceae and has large number of taxa having economic and ecological importance. The aim of the study was to analyze highly medicinal products as well, antioxidant activity and genetic variability in three species of Artemisia from various sites of district Poonch. For the evaluation of phytochemicals and antioxidants, different volumes of extracts and solvents were used from leaves. The species were analyzed for genetic diversity within and between various Artemisia species. For molecular investigation, random amplification of polymorphic DNA (RAPD) fragment was used to determine genetic variations between and within species. From of ten primers only six primers gave amplified products. By using all six primers, total 331 bands were amplified from which 30 were monomorphic, 301 were polymorphic and percentage of polymorphism was 91% in Artemisia japonica, 321 bands were amplified in Artemisia roxberghiana from which 21 were monomorphic, 3...

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