samah khattab - Academia.edu (original) (raw)
Papers by samah khattab
Introduction: The abuse of anabolic-androgenic steroids (AAS) has recently emerged as a major for... more Introduction: The abuse of anabolic-androgenic steroids (AAS) has recently emerged as a major form of substance abuse worldwide. Chronic AAS use suppresses the function of the hypothalamic-pituitary-gonadal axis, which may lead to hypogonadism and thus infertility. Aim of the work: This study was performed to evaluate the potential effects of methandrostenolone (dianabol), nandrolone decanoate (nandrolone) separately and in combination on reproductive system of adult male albino rats and their recovery response. Material and Methods: 72 adult male albino rats were divided into 4 groups: group I; control group, group II; treated with dianabol only, group III; treated with nandrolone only, group IV; treated with both nandrolone and dianabol. Treatments were given for 8 weeks followed by 4 weeks recovery. Hormonal level assay [Testosterone, Follicle Stimulating Hormone (FSH) and Lutenizing Hormone (LH)], semen analysis, histopathological and immunohistochemical examinations were perfor...
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](Toxicology Mechanisms and Methods
Abstract The major sources for human exposure to Benzo [a] pyrene (B[a]P) are contaminated food, ... more Abstract The major sources for human exposure to Benzo [a] pyrene (B[a]P) are contaminated food, water, and inhalation of polycyclic aromatic hydrocarbon. B[a]P is a well-known human genotoxic carcinogen (IARC Group 1). It has a tumorigenic potential in virtually all in vivo experimental animal model systems. The study aimed to evaluate the effect of catechin hydrate (CH) against B [a] P-induced toxicity in the lung of adult albino rats. Thirty-six adult male albino rats (150–200 g) were divided into six groups, three control groups, and three experimental groups: B[a] P-treated group, (CH)-treated group, and B[a] P+(CH)-treated group. At the end of the fourth week of the study, blood samples and lung tissues were obtained for the biochemical and genotoxicity, RT-PCR, histopathological, and immunohistochemical investigations, respectively. Our results clarified that B[a] P exposure caused a subsequent decrease in the activities of antioxidant enzymes (SOD, CAT), and conversely (MDA) levels elevated markedly. Also, B[a] P induced DNA damages and activated the apoptotic pathway, presented by upregulated Bax, caspase-3, and downregulated Bcl-2 gens. However, treatment with CH increased antioxidant enzymes as well as regulated apoptosis. Discernible histological changes in the lung also supported the protective effects of CH. These findings suggested that CH is an effective natural product that attenuates Benzo [a] pyrene-induced lung toxicity.
Toxicology Research
Benzo [a] pyrene (B[a]P) is a potent mutagen and carcinogen, considered one of the commonest conc... more Benzo [a] pyrene (B[a]P) is a potent mutagen and carcinogen, considered one of the commonest concomitants in the environment. The study aimed to evaluate the effect of catechin hydrate on benzo pyrene-induced kidney toxicity. Thirty-six adult male albino rats were divided into six groups: group I untreated control, group II received 10 mL/kg of corn oil (solvent of benzo [a] pyrene) twice a week, group III received 1 mL/kg 0.5% dimethyl sulfoxide (DMSO) (solvent of catechin) once per day, group IV received 50 mg/kg body weight of benzo[a]pyrene twice a week, group V received 20 mg/kg body weight of catechin in 1 mL/kg 0.5% DMSO once daily, and group VI received both catechin+benzo [a] pyrene with the same doses. All treatment was given by oral gavage for four weeks. At the end of the experiment, blood samples were collected for biochemical investigations, tissues were obtained for genotoxicity, RT-PCR, and histopathological studies. B[a]P exposure induced an increase in serum urea a...
Introduction: The abuse of anabolic-androgenic steroids (AAS) has recently emerged as a major for... more Introduction: The abuse of anabolic-androgenic steroids (AAS) has recently emerged as a major form of substance abuse worldwide. Chronic AAS use suppresses the function of the hypothalamic-pituitary-gonadal axis, which may lead to hypogonadism and thus infertility. Aim of the work: This study was performed to evaluate the potential effects of methandrostenolone (dianabol), nandrolone decanoate (nandrolone) separately and in combination on reproductive system of adult male albino rats and their recovery response. Material and Methods: 72 adult male albino rats were divided into 4 groups: group I; control group, group II; treated with dianabol only, group III; treated with nandrolone only, group IV; treated with both nandrolone and dianabol. Treatments were given for 8 weeks followed by 4 weeks recovery. Hormonal level assay [Testosterone, Follicle Stimulating Hormone (FSH) and Lutenizing Hormone (LH)], semen analysis, histopathological and immunohistochemical examinations were perfor...
Toxicology Mechanisms and Methods
Abstract The major sources for human exposure to Benzo [a] pyrene (B[a]P) are contaminated food, ... more Abstract The major sources for human exposure to Benzo [a] pyrene (B[a]P) are contaminated food, water, and inhalation of polycyclic aromatic hydrocarbon. B[a]P is a well-known human genotoxic carcinogen (IARC Group 1). It has a tumorigenic potential in virtually all in vivo experimental animal model systems. The study aimed to evaluate the effect of catechin hydrate (CH) against B [a] P-induced toxicity in the lung of adult albino rats. Thirty-six adult male albino rats (150–200 g) were divided into six groups, three control groups, and three experimental groups: B[a] P-treated group, (CH)-treated group, and B[a] P+(CH)-treated group. At the end of the fourth week of the study, blood samples and lung tissues were obtained for the biochemical and genotoxicity, RT-PCR, histopathological, and immunohistochemical investigations, respectively. Our results clarified that B[a] P exposure caused a subsequent decrease in the activities of antioxidant enzymes (SOD, CAT), and conversely (MDA) levels elevated markedly. Also, B[a] P induced DNA damages and activated the apoptotic pathway, presented by upregulated Bax, caspase-3, and downregulated Bcl-2 gens. However, treatment with CH increased antioxidant enzymes as well as regulated apoptosis. Discernible histological changes in the lung also supported the protective effects of CH. These findings suggested that CH is an effective natural product that attenuates Benzo [a] pyrene-induced lung toxicity.
Toxicology Research
Benzo [a] pyrene (B[a]P) is a potent mutagen and carcinogen, considered one of the commonest conc... more Benzo [a] pyrene (B[a]P) is a potent mutagen and carcinogen, considered one of the commonest concomitants in the environment. The study aimed to evaluate the effect of catechin hydrate on benzo pyrene-induced kidney toxicity. Thirty-six adult male albino rats were divided into six groups: group I untreated control, group II received 10 mL/kg of corn oil (solvent of benzo [a] pyrene) twice a week, group III received 1 mL/kg 0.5% dimethyl sulfoxide (DMSO) (solvent of catechin) once per day, group IV received 50 mg/kg body weight of benzo[a]pyrene twice a week, group V received 20 mg/kg body weight of catechin in 1 mL/kg 0.5% DMSO once daily, and group VI received both catechin+benzo [a] pyrene with the same doses. All treatment was given by oral gavage for four weeks. At the end of the experiment, blood samples were collected for biochemical investigations, tissues were obtained for genotoxicity, RT-PCR, and histopathological studies. B[a]P exposure induced an increase in serum urea a...