Ana Blandino - Academia.edu (original) (raw)

Papers by Ana Blandino

DOAJ (DOAJ: Directory of Open Access Journals), Jun 1, 2022

Bioresource Technology, 2017

Fermentation

The production of bioplastics from renewable materials has gained interest in recent years, due t... more The production of bioplastics from renewable materials has gained interest in recent years, due to the large accumulation of non-degradable plastic produced in the environment. Here, sugar beet pulp (SBP) is evaluated as a potential raw material for the production of bioplastics such as polylactic acid (PLA) and polyhydroxyalkanoates (PHAs). SBP is a by-product obtained in the sugar industry after sugar extraction from sugar beet, and it is mainly used for animal feed. It has a varied composition consisting mainly of cellulose, hemicellulose and pectin. Thus, it has been used to produce different value-added products such as methane, hydrogen, pectin, simple sugars, ethanol, lactic acid and succinic acid. This review focuses on the different bioprocesses involved in the production of lactic acid and PHAs, both precursors of bioplastics, from sugars derived from SBP. The review, therefore, describes the pretreatments applied to SBP, the conditions most frequently used for the enzymat...

Bioresource Technology, 2017

Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the pr... more Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the production of bio-products. Enzyme hydrolysis with the combined effect of mainly cellulases, xylanases and pectinases, turned out to be very efficient, obtaining almost double the concentration of sugars measured with the sole action of Celluclast® and βglucosidase, and increasing 5 times the hydrolysis rate. As the sole pretreatment, ESBC soaked in the hydrolysis buffer were autoclaved, avoiding the application of severe conventional biomass pretreatments. Moreover, a promising alternative for the complete utilization of glucose, xylose, arabinose, mannose and maltose contained in ESBC is proposed in this paper. It consists of sequential fermentation of sugars released in the hydrolysis step to produce bioethanol and lactic acid as main bio-products. Compared to separate fermentations, with this strategy glucose and hemicellulose derived sugars were completely consumed and the 44 % of pectin derived sugars.

Journal of the Science of Food and Agriculture

BackgroundOrange peels can serve as a cost‐effective raw material for the production of lactic ac... more BackgroundOrange peels can serve as a cost‐effective raw material for the production of lactic acid. Indeed, given their high concentration of carbohydrates and low content of lignin, they represent an important source of fermentable sugars, recoverable after a hydrolytic step.ResultsIn the present article, the fermented solid, obtained after 5 days of Aspergillus awamori growth, was used as the only source of enzymes, mainly composed of xylanase (40.6 IU g−1 of dried washed orange peels) and exo‐polygalacturonase (16.3 IU g−1 of dried washed orange peels) activities. After the hydrolysis, the highest concentration of reducing sugars (24.4 g L−1) was achieved with 20% fermented and 80% non‐fermented orange peels. The hydrolysate was fermented with three lactic acid bacteria strains (Lacticaseibacillus casei 2246 and 2240 and Lacticaseibacillus rhamnosus 1019) which demonstrated good growth ability. The yeast extract supplementation increased the lactic acid production rate and yield...

Biofuels, Bioproducts and Biorefining

Applied Microbiology and Biotechnology

Rugulopteryx okamurae is an invasive brown alga causing severe environmental and economic problem... more Rugulopteryx okamurae is an invasive brown alga causing severe environmental and economic problems on the western Mediterranean coasts. Thus, in addition to the difficulties caused to the fishing and tourism sectors, there is a need to manage its accumulation on the beaches. This work aims to valorise this waste by using it as raw material for producing monosaccharides through a two-stage sequential process. These sugars could be used for different fermentative processes to obtain high-value-added bioproducts. In this work, biological pretreatment of the previously conditioned seaweed with the fungus Aspergillus awamori in solid-state fermentation (SSF), followed by enzymatic hydrolysis with a commercial enzyme cocktail, was performed. The effect of the extension of the biological pretreatment (2, 5, 8 and 12 days) on the subsequent release of total reducing sugars (TRS) in the enzymatic hydrolysis stage was studied. To analyse this effect, experimental data of TRS produced along th...

Foods, 2022

The agri-food sector produces large amounts of waste annually, most of which is lignocellulosic b... more The agri-food sector produces large amounts of waste annually, most of which is lignocellulosic biomass [...]

Waste Management & Research: The Journal for a Sustainable Circular Economy, 2018

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, the... more Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydr...

Foods, 2020

In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as... more In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as a generic microbial culture medium, was carried out by using exhausted sugar beet pulp pellets (ESBPPs) as raw material. For this purpose, the hydrolysis was performed through the direct addition of the fermented ESBPPs obtained by fungal solid-state fermentation (SSF) as an enzyme source. By directly using this fermented solid, the stages for enzyme extraction and purification were avoided. The effects of temperature, fermented to fresh solid ratio, supplementation of fermented ESBPP with commercial cellulase, and the use of high-solid fed-batch enzymatic hydrolysis were studied to obtain the maximum reducing sugar (RS) concentration and productivity. The highest RS concentration and productivity, 127.3 g·L−1 and 24.3 g·L−1·h−1 respectively, were obtained at 50 °C and with an initial supplementation of 2.17 U of Celluclast® per gram of dried solid in fed-batch mode. This process was ca...

Food Research International, 2003

Chemical engineering transactions, 2009

Previous studies demonstrated that Aspergillus awamori produces pectinases, xylanases and cellula... more Previous studies demonstrated that Aspergillus awamori produces pectinases, xylanases and cellulases by solid state fermentation. In order to evaluate the production of these enzymes in a laboratory-scale bioreactor, a rotating drum fermentor was used. Different air flow rates were tested demonstrating a positive effect of this parameter on enzyme activity. The production of the highest enzyme activity level was attained working in static or with an agitation of 1 min/day, using an aeration of 120 mL/min.

Chemical engineering transactions, 2009

Chemical engineering transactions, 2009

Many microorganisms that decompose lignocellulosic material are being studied as producers of enz... more Many microorganisms that decompose lignocellulosic material are being studied as producers of enzymes to perform enzymatic hydrolysis of the lignocellulosic material present in residues from the agroindustries. Grape pomace is the residue left after juice extraction from the grapes in the wine making industry, but it is under-exploited and most of it is generally disposed in open areas, leading to serious environmental problems. Citrus peels are the main solid by-product of the citrus processing industry and constitute about 50% of fresh fruit weight. The disposal of the fresh peels is also becoming a major problem in many factories. As an alternative to the disposal of both residues, in this work the production of xylanase, exo-polygalacturonase and CMCase has been studied by using a mixture 1:1 (w/w) of grape pomace and orange peels as solid substrate for the fermentation of the fungus Aspergillus awamori. A solid state glass-column reactor with forced air at lab scale was used fo...

Bioethanol Production from Food Crops

Abstract The chemical composition of sugar beet roots makes this raw material an attractive feeds... more Abstract The chemical composition of sugar beet roots makes this raw material an attractive feedstock for ethanol fermentation. In the manufacture of sugar from this crop, various intermediates, by-products, and wastes are generated, which can be used for the production of energy and other value-added products, such as biofuels. In this chapter, the current processes for the conversion of the sugary juices (raw, thin, and thick juices), molasses and sugar beet pulp into bioethanol are described. In addition, the economics of sugar beet ethanol and the principal strategies proposed for increasing bioethanol yields are described.

Advances in Food Biotechnology, 2015

DOAJ (DOAJ: Directory of Open Access Journals), Jun 1, 2022

Bioresource Technology, 2017

Fermentation

The production of bioplastics from renewable materials has gained interest in recent years, due t... more The production of bioplastics from renewable materials has gained interest in recent years, due to the large accumulation of non-degradable plastic produced in the environment. Here, sugar beet pulp (SBP) is evaluated as a potential raw material for the production of bioplastics such as polylactic acid (PLA) and polyhydroxyalkanoates (PHAs). SBP is a by-product obtained in the sugar industry after sugar extraction from sugar beet, and it is mainly used for animal feed. It has a varied composition consisting mainly of cellulose, hemicellulose and pectin. Thus, it has been used to produce different value-added products such as methane, hydrogen, pectin, simple sugars, ethanol, lactic acid and succinic acid. This review focuses on the different bioprocesses involved in the production of lactic acid and PHAs, both precursors of bioplastics, from sugars derived from SBP. The review, therefore, describes the pretreatments applied to SBP, the conditions most frequently used for the enzymat...

Bioresource Technology, 2017

Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the pr... more Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the production of bio-products. Enzyme hydrolysis with the combined effect of mainly cellulases, xylanases and pectinases, turned out to be very efficient, obtaining almost double the concentration of sugars measured with the sole action of Celluclast® and βglucosidase, and increasing 5 times the hydrolysis rate. As the sole pretreatment, ESBC soaked in the hydrolysis buffer were autoclaved, avoiding the application of severe conventional biomass pretreatments. Moreover, a promising alternative for the complete utilization of glucose, xylose, arabinose, mannose and maltose contained in ESBC is proposed in this paper. It consists of sequential fermentation of sugars released in the hydrolysis step to produce bioethanol and lactic acid as main bio-products. Compared to separate fermentations, with this strategy glucose and hemicellulose derived sugars were completely consumed and the 44 % of pectin derived sugars.

Journal of the Science of Food and Agriculture

BackgroundOrange peels can serve as a cost‐effective raw material for the production of lactic ac... more BackgroundOrange peels can serve as a cost‐effective raw material for the production of lactic acid. Indeed, given their high concentration of carbohydrates and low content of lignin, they represent an important source of fermentable sugars, recoverable after a hydrolytic step.ResultsIn the present article, the fermented solid, obtained after 5 days of Aspergillus awamori growth, was used as the only source of enzymes, mainly composed of xylanase (40.6 IU g−1 of dried washed orange peels) and exo‐polygalacturonase (16.3 IU g−1 of dried washed orange peels) activities. After the hydrolysis, the highest concentration of reducing sugars (24.4 g L−1) was achieved with 20% fermented and 80% non‐fermented orange peels. The hydrolysate was fermented with three lactic acid bacteria strains (Lacticaseibacillus casei 2246 and 2240 and Lacticaseibacillus rhamnosus 1019) which demonstrated good growth ability. The yeast extract supplementation increased the lactic acid production rate and yield...

Biofuels, Bioproducts and Biorefining

Applied Microbiology and Biotechnology

Rugulopteryx okamurae is an invasive brown alga causing severe environmental and economic problem... more Rugulopteryx okamurae is an invasive brown alga causing severe environmental and economic problems on the western Mediterranean coasts. Thus, in addition to the difficulties caused to the fishing and tourism sectors, there is a need to manage its accumulation on the beaches. This work aims to valorise this waste by using it as raw material for producing monosaccharides through a two-stage sequential process. These sugars could be used for different fermentative processes to obtain high-value-added bioproducts. In this work, biological pretreatment of the previously conditioned seaweed with the fungus Aspergillus awamori in solid-state fermentation (SSF), followed by enzymatic hydrolysis with a commercial enzyme cocktail, was performed. The effect of the extension of the biological pretreatment (2, 5, 8 and 12 days) on the subsequent release of total reducing sugars (TRS) in the enzymatic hydrolysis stage was studied. To analyse this effect, experimental data of TRS produced along th...

Foods, 2022

The agri-food sector produces large amounts of waste annually, most of which is lignocellulosic b... more The agri-food sector produces large amounts of waste annually, most of which is lignocellulosic biomass [...]

Waste Management & Research: The Journal for a Sustainable Circular Economy, 2018

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, the... more Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydr...

Foods, 2020

In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as... more In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as a generic microbial culture medium, was carried out by using exhausted sugar beet pulp pellets (ESBPPs) as raw material. For this purpose, the hydrolysis was performed through the direct addition of the fermented ESBPPs obtained by fungal solid-state fermentation (SSF) as an enzyme source. By directly using this fermented solid, the stages for enzyme extraction and purification were avoided. The effects of temperature, fermented to fresh solid ratio, supplementation of fermented ESBPP with commercial cellulase, and the use of high-solid fed-batch enzymatic hydrolysis were studied to obtain the maximum reducing sugar (RS) concentration and productivity. The highest RS concentration and productivity, 127.3 g·L−1 and 24.3 g·L−1·h−1 respectively, were obtained at 50 °C and with an initial supplementation of 2.17 U of Celluclast® per gram of dried solid in fed-batch mode. This process was ca...

Food Research International, 2003

Chemical engineering transactions, 2009

Previous studies demonstrated that Aspergillus awamori produces pectinases, xylanases and cellula... more Previous studies demonstrated that Aspergillus awamori produces pectinases, xylanases and cellulases by solid state fermentation. In order to evaluate the production of these enzymes in a laboratory-scale bioreactor, a rotating drum fermentor was used. Different air flow rates were tested demonstrating a positive effect of this parameter on enzyme activity. The production of the highest enzyme activity level was attained working in static or with an agitation of 1 min/day, using an aeration of 120 mL/min.

Chemical engineering transactions, 2009

Chemical engineering transactions, 2009

Many microorganisms that decompose lignocellulosic material are being studied as producers of enz... more Many microorganisms that decompose lignocellulosic material are being studied as producers of enzymes to perform enzymatic hydrolysis of the lignocellulosic material present in residues from the agroindustries. Grape pomace is the residue left after juice extraction from the grapes in the wine making industry, but it is under-exploited and most of it is generally disposed in open areas, leading to serious environmental problems. Citrus peels are the main solid by-product of the citrus processing industry and constitute about 50% of fresh fruit weight. The disposal of the fresh peels is also becoming a major problem in many factories. As an alternative to the disposal of both residues, in this work the production of xylanase, exo-polygalacturonase and CMCase has been studied by using a mixture 1:1 (w/w) of grape pomace and orange peels as solid substrate for the fermentation of the fungus Aspergillus awamori. A solid state glass-column reactor with forced air at lab scale was used fo...

Bioethanol Production from Food Crops

Abstract The chemical composition of sugar beet roots makes this raw material an attractive feeds... more Abstract The chemical composition of sugar beet roots makes this raw material an attractive feedstock for ethanol fermentation. In the manufacture of sugar from this crop, various intermediates, by-products, and wastes are generated, which can be used for the production of energy and other value-added products, such as biofuels. In this chapter, the current processes for the conversion of the sugary juices (raw, thin, and thick juices), molasses and sugar beet pulp into bioethanol are described. In addition, the economics of sugar beet ethanol and the principal strategies proposed for increasing bioethanol yields are described.

Advances in Food Biotechnology, 2015