Ana Carrasco - Academia.edu (original) (raw)

Papers by Ana Carrasco

Research paper thumbnail of Densities and Viscosities of the Ternary Mixtures 2Methyl1-propanol (or 2Methyl2-propanol) + N Hexane + 1-Chlorobutane at 298.15 K

Journal of Chemical and Engineering Data, 2008

The excess molar volumes, V E , and viscosity deviations, ∆η, were calculated from the measured d... more The excess molar volumes, V E , and viscosity deviations, ∆η, were calculated from the measured density and viscosity values over the whole miscibility composition ranges for the ternary systems water + butyl acetate + methanol and water + ethyl propionate + methanol and their constituent binaries, at 303.15 K and atmospheric pressure. A Redlich-Kister type equation was used to correlate binary V E and ∆η data, as well as the ternary data. This equation was used to calculate the above referred properties along the binodal curve.

Research paper thumbnail of Wild Solanum species as resistance sources against different pathogens of potato

Potato Research, 1998

Resistance screenings againstPhytophthora infestans, Globodera pallida Pa2/3 and the viruses PVYN... more Resistance screenings againstPhytophthora infestans, Globodera pallida Pa2/3 and the viruses PVYN, PLRV, PVM and PVS were performed in 98 accessions belonging to 90Solanum wild species. Seventy accessions showed resistance or partial resistance to one or several of these pathogens. Among themS. brachistotrichum exhibited combined resistance to nematodes, late blight and some of the viruses evaluated. Only part of the resistances previously found in different wild species could be confirmed, which might be due to the existing variability within species, the use of different pathogen strains or isolates, or to different methodologies for resistance screening. Several new resistance sources, such asS. andreanum, S. maglia. S. doddsii andS. boliviense, are provided for the different pathogens analyzed. These findings demonstrate that within the gene pool ofSolanum species, still numerous but in part unexploited resistances are available which could be favourably used in potato breeding.

Research paper thumbnail of Transfer of PLRV resistance from Solanum verrucosum Schlechdt to potato ( S. tuberosum L.) by protoplast electrofusion

Potato Research, 2000

Somatic fusions between an accession of the diploid wild speciesSolanum verrucosum and a dihaploi... more Somatic fusions between an accession of the diploid wild speciesSolanum verrucosum and a dihaploid S.tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out of 16 regenerants were obtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics, chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV.

Research paper thumbnail of Marker-assisted Breeding for Disease Resistance in Potato

Potato Research, 2009

Sub-project 5 of BIOEXPLOIT aims to design durable disease resistance through marker-assisted bre... more Sub-project 5 of BIOEXPLOIT aims to design durable disease resistance through marker-assisted breeding by converting existing markers for high-throughput application, developing and validating high-throughput marker technologies and pyramiding major R genes and/or quantitative trait loci into elite material. Activities include (1) the fine mapping of the quantitative trait locus PiXspg which accounts for a large proportion of the variation in late blight resistance, (2) converting SNP-based markers and an AFLP marker to easy-to-use-markers, (3) testing of progenies with combined sources of late blight resistance for presence of R genes and agronomic features, (4) backcrossing new sources of resistance to S. tuberosum and molecular screening of breeding materials with marker GP94 linked with gene Rpi-phu1 conferring late blight resistance, (5) evaluating potato clones with enhanced resistance against Phytophthora infestans under field conditions of Toluca (México), and (6) developing populations and marker-assisted breeding for disease resistance.

Research paper thumbnail of Germplasm enhancement with wild tuber-bearing species: introgression of PVY resistance and high dry matter content from Solanum berthaultii, S. gourlayi, S. tarijense and S. vernei

Potato Research, 2005

The narrow genetic base of the cultivated potato is a severe constraint for potato breeding. Wild... more The narrow genetic base of the cultivated potato is a severe constraint for potato breeding. Wild tuber-bearing species constitute an important source of genes for resistance to diseases and pests as well as abiotic factors. Seven interspecific hybrid populations were generated from crosses between diploidTuberosum clones and four wild species:Solanum berthaultii, S. gourlayi, S. tarijense andS. vernei, and evaluated in a field experiment in Burgos (Spain) as a way to broaden the genetic base of the cultivated potato. Good tuberization ability and great variability for yield within the different families were detected. Hybridization with the wild species resulted in high levels of PVY resistance inS. berthaultii andS. vernei hybrids and a large increase of dry matter content in all populations except theS. berthaultii hybrids.

Research paper thumbnail of Densities and Viscosities of the Ternary Mixtures 2Methyl1-propanol (or 2Methyl2-propanol) + N Hexane + 1-Chlorobutane at 298.15 K

Journal of Chemical and Engineering Data, 2008

The excess molar volumes, V E , and viscosity deviations, ∆η, were calculated from the measured d... more The excess molar volumes, V E , and viscosity deviations, ∆η, were calculated from the measured density and viscosity values over the whole miscibility composition ranges for the ternary systems water + butyl acetate + methanol and water + ethyl propionate + methanol and their constituent binaries, at 303.15 K and atmospheric pressure. A Redlich-Kister type equation was used to correlate binary V E and ∆η data, as well as the ternary data. This equation was used to calculate the above referred properties along the binodal curve.

Research paper thumbnail of Wild Solanum species as resistance sources against different pathogens of potato

Potato Research, 1998

Resistance screenings againstPhytophthora infestans, Globodera pallida Pa2/3 and the viruses PVYN... more Resistance screenings againstPhytophthora infestans, Globodera pallida Pa2/3 and the viruses PVYN, PLRV, PVM and PVS were performed in 98 accessions belonging to 90Solanum wild species. Seventy accessions showed resistance or partial resistance to one or several of these pathogens. Among themS. brachistotrichum exhibited combined resistance to nematodes, late blight and some of the viruses evaluated. Only part of the resistances previously found in different wild species could be confirmed, which might be due to the existing variability within species, the use of different pathogen strains or isolates, or to different methodologies for resistance screening. Several new resistance sources, such asS. andreanum, S. maglia. S. doddsii andS. boliviense, are provided for the different pathogens analyzed. These findings demonstrate that within the gene pool ofSolanum species, still numerous but in part unexploited resistances are available which could be favourably used in potato breeding.

Research paper thumbnail of Transfer of PLRV resistance from Solanum verrucosum Schlechdt to potato ( S. tuberosum L.) by protoplast electrofusion

Potato Research, 2000

Somatic fusions between an accession of the diploid wild speciesSolanum verrucosum and a dihaploi... more Somatic fusions between an accession of the diploid wild speciesSolanum verrucosum and a dihaploid S.tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out of 16 regenerants were obtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics, chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV.

Research paper thumbnail of Marker-assisted Breeding for Disease Resistance in Potato

Potato Research, 2009

Sub-project 5 of BIOEXPLOIT aims to design durable disease resistance through marker-assisted bre... more Sub-project 5 of BIOEXPLOIT aims to design durable disease resistance through marker-assisted breeding by converting existing markers for high-throughput application, developing and validating high-throughput marker technologies and pyramiding major R genes and/or quantitative trait loci into elite material. Activities include (1) the fine mapping of the quantitative trait locus PiXspg which accounts for a large proportion of the variation in late blight resistance, (2) converting SNP-based markers and an AFLP marker to easy-to-use-markers, (3) testing of progenies with combined sources of late blight resistance for presence of R genes and agronomic features, (4) backcrossing new sources of resistance to S. tuberosum and molecular screening of breeding materials with marker GP94 linked with gene Rpi-phu1 conferring late blight resistance, (5) evaluating potato clones with enhanced resistance against Phytophthora infestans under field conditions of Toluca (México), and (6) developing populations and marker-assisted breeding for disease resistance.

Research paper thumbnail of Germplasm enhancement with wild tuber-bearing species: introgression of PVY resistance and high dry matter content from Solanum berthaultii, S. gourlayi, S. tarijense and S. vernei

Potato Research, 2005

The narrow genetic base of the cultivated potato is a severe constraint for potato breeding. Wild... more The narrow genetic base of the cultivated potato is a severe constraint for potato breeding. Wild tuber-bearing species constitute an important source of genes for resistance to diseases and pests as well as abiotic factors. Seven interspecific hybrid populations were generated from crosses between diploidTuberosum clones and four wild species:Solanum berthaultii, S. gourlayi, S. tarijense andS. vernei, and evaluated in a field experiment in Burgos (Spain) as a way to broaden the genetic base of the cultivated potato. Good tuberization ability and great variability for yield within the different families were detected. Hybridization with the wild species resulted in high levels of PVY resistance inS. berthaultii andS. vernei hybrids and a large increase of dry matter content in all populations except theS. berthaultii hybrids.