Ana Maria Blandino Garrido - Academia.edu (original) (raw)
Papers by Ana Maria Blandino Garrido
Bioresource Technology, 2017
Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the pr... more Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the production of bio-products. Enzyme hydrolysis with the combined effect of mainly cellulases, xylanases and pectinases, turned out to be very efficient, obtaining almost double the concentration of sugars measured with the sole action of Celluclast® and βglucosidase, and increasing 5 times the hydrolysis rate. As the sole pretreatment, ESBC soaked in the hydrolysis buffer were autoclaved, avoiding the application of severe conventional biomass pretreatments. Moreover, a promising alternative for the complete utilization of glucose, xylose, arabinose, mannose and maltose contained in ESBC is proposed in this paper. It consists of sequential fermentation of sugars released in the hydrolysis step to produce bioethanol and lactic acid as main bio-products. Compared to separate fermentations, with this strategy glucose and hemicellulose derived sugars were completely consumed and the 44 % of pectin derived sugars.
Foods, 2020
In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as... more In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as a generic microbial culture medium, was carried out by using exhausted sugar beet pulp pellets (ESBPPs) as raw material. For this purpose, the hydrolysis was performed through the direct addition of the fermented ESBPPs obtained by fungal solid-state fermentation (SSF) as an enzyme source. By directly using this fermented solid, the stages for enzyme extraction and purification were avoided. The effects of temperature, fermented to fresh solid ratio, supplementation of fermented ESBPP with commercial cellulase, and the use of high-solid fed-batch enzymatic hydrolysis were studied to obtain the maximum reducing sugar (RS) concentration and productivity. The highest RS concentration and productivity, 127.3 g·L−1 and 24.3 g·L−1·h−1 respectively, were obtained at 50 °C and with an initial supplementation of 2.17 U of Celluclast® per gram of dried solid in fed-batch mode. This process was ca...
Foods, 2022
The agri-food sector produces large amounts of waste annually, most of which is lignocellulosic b... more The agri-food sector produces large amounts of waste annually, most of which is lignocellulosic biomass [...]
Bioethanol Production from Food Crops, 2019
Abstract The chemical composition of sugar beet roots makes this raw material an attractive feeds... more Abstract The chemical composition of sugar beet roots makes this raw material an attractive feedstock for ethanol fermentation. In the manufacture of sugar from this crop, various intermediates, by-products, and wastes are generated, which can be used for the production of energy and other value-added products, such as biofuels. In this chapter, the current processes for the conversion of the sugary juices (raw, thin, and thick juices), molasses and sugar beet pulp into bioethanol are described. In addition, the economics of sugar beet ethanol and the principal strategies proposed for increasing bioethanol yields are described.
Foods, 2021
Exhausted sugar beet pulp (ESBP), a by-product of the sugar industry, has been used as a substrat... more Exhausted sugar beet pulp (ESBP), a by-product of the sugar industry, has been used as a substrate to produce lactic acid (LA). Due to the fact that ESBP contains a high percentage of pectin and hemicellulose, different pretreatments were studied to solubilize them and to facilitate the access to cellulose in the subsequent enzymatic hydrolysis. Several pretreatments were studied, specifically biological, oxidant with alkaline hydrogen peroxide (AHP), and thermochemical with acid (0.25, 0.5, or 1% w/v of H2SO4). Pretreated ESBP was enzymatically hydrolysed and fermented with the strain Lactiplantibacillus plantarum for LA production. The hydrolysis was carried out with the commercial enzymes Celluclast®, pectinase, and xylanase, for 48 h. After that, the hydrolysate was supplemented with yeast extract and calcium carbonate before the bacteria inoculation. Results showed that all the pretreatments caused a modification of the fibre composition of ESBP. In most cases, the cellulose co...
Journal of the Science of Food and Agriculture, 2020
BACKGROUNDExhausted sugar beet pulp pellets (ESBPP) were used as raw material for lactic acid (LA... more BACKGROUNDExhausted sugar beet pulp pellets (ESBPP) were used as raw material for lactic acid (LA) fermentation. The enzymatic hydrolysis of ESBPP was performed with the solid obtained after the fungal solid‐state fermentation of ESBPP as a source of hydrolytic enzymes. Subsequently, a medium rich in glucose and arabinose was obtained, which was used to produce LA by fermentation. For LA production, two Lactobacillus strains were assayed and the effects of the supplementation of the hydrolysate with a nitrogen source and the mode of pH regulation of the fermentation were investigated. Moreover, a kinetic model for LA fermentation by Lactobacillus plantarum of ESBPP hydrolysates was developed.RESULTSL. plantarum produced a LA concentration 34% higher than that produced by L. casei. The highest LA concentration (30 g L−1) was obtained with L. plantarum when the hydrolysate was supplemented with 5 g L−1 yeast extract and the pH was controlled with CaCO3. The concentration of acetic aci...
Journal of the Science of Food and Agriculture, 2020
BACKGROUNDExhausted sugar beet pulp pellets (ESBPP), a sugar industry by‐product generated after ... more BACKGROUNDExhausted sugar beet pulp pellets (ESBPP), a sugar industry by‐product generated after sugar extraction in the sugar production process, have been used as a raw material for lactic acid (LA) production via hydrolysis and fermentation by Lactobacillus casei. To design a more cost‐effective process, simultaneous saccharification and fermentation (SSF) of ESBPP is proposed in the present study. The effects of pH control, nutrient supplementation and solid addition in fed‐batch SSF on lactic acid production were investigated.RESULTSThe highest LA concentration (26.88 g L–1) was reached in fed‐batch SSF at a solid/liquid loading of 0.2 g mL–1, with pH control (by adding 30 g L–1 CaCO3 to the medium) and nutrient supplementation (by adding 20 mL of MRS medium per 100 mL of buffer). Under these conditions, a maximum productivity of 0.63 g L−1 h−1 was achieved, which is 2.7 times higher than that attained in the control experiment (SSF inoculated at time 0 h). However, a slightly ...
Journal of the Science of Food and Agriculture, 2019
Background: Considering the large amounts of by-products derived from orange processing, generall... more Background: Considering the large amounts of by-products derived from orange processing, generally discarded, the aim of this article was to explore the feasibility of using orange peels for lactic acid production in solid state fermentation. Results: Different species of lactic acid bacteria were employed singly and in co-culture, in order to evaluate their ability to ferment orange peels and produce lactic acid. Among the single cultures tested, Lactobacillus casei 2246 was the most efficient strain, reaching the highest concentration of lactic acid (209.65 g/Kg) and yield (0.88 g/g). The employment of Lactobacillus plantarum 285 and Lactobacillus paracasei 4186 in co-culture, produced a comparable amount of lactic acid, showing better performance than the same strains in single cultures. Conclusion: Orange peels represent a suitable raw material for solid state fermentation employing lactic acid bacteria. Lactic acid was obtained consuming the most of sugars available leading to high yields. Despite all the strains tested showed the same growth ability, different peculiarities in lactic acid production depending on the species/strains were revealed suggesting the relevance on strains selection.
Waste Management & Research: The Journal for a Sustainable Circular Economy, 2018
Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, the... more Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydr...
Biofuels, Bioproducts and Biorefining, 2019
In this work, a mathematical model is proposed to predict the kinetic behaviour of the enzymatic ... more In this work, a mathematical model is proposed to predict the kinetic behaviour of the enzymatic conversion of various types of lignocellulosic biomass into fermentable sugars. Digestion of the cellulosic polymers is carried out using enzymatic hydrolysis under different conditions. Unlike other kinetic models, published previously for this process, this one considers the heterogeneous nature of the process by which a solid, in the form of small particles, is decomposed to monosaccharides by the action of a diverse set of enzymes in solution. The effect of the particle size on the hydrolysis rate has also been taken into consideration. To assess the model's goodness of fit to any general situation, the experimental data obtained in the hydrolysis of three different lignocellulosic residues have been analysed. Thus, the hydrolysis data of wheat straw, rice husks and exhausted sugar beet pellets have been compared with the theoretical values calculated by the model. The results obtained show that this model predicts the enzyme's hydrolysis of lignocellulosic substrates under different conditions very accurately and it could therefore be used efficiently in the optimization of the hydrolysis processes implemented in the bio-refinery industry.
Trends in Food Science & Technology, 2018
Background: Globally, significant amounts of agro-food residues are generated, most of which are ... more Background: Globally, significant amounts of agro-food residues are generated, most of which are burned as waste disposal. Given the abundance of this biomass, it can be used as raw material for the production of biofuels and valuable chemicals through biochemical conversion. Research in this field has gained importance given concerns regarding limitation of fossil resources, environmental problems and sustainability and preference towards natural, biodegradable and environmentally friendly products. Furthermore, the accessibility of alternative substrate sources for fermentative production of these high-value products has been one of the primary goals of industrial biotechnology research in the past two decades. Scope and approach: The present review is focused in the production of high value products through microbial fermentation of sugars from agro-food residues. Research in this field is interesting due to concerns regarding limitation of fossil resources. On the one hand, for the production of these bioproducts it is important the accessibility of alternative substrate and, on the other hand, the selection of the right microorganism to obtain the desired product. For this reason, the novelty of the present work is that details all different bioproducts which can be obtained through fermentation of sugars derived from agro-food residues, describing raw materials, pretreatments, mode of operation, conditions and microorganisms used, together with the production yields.
Applied microbiology and biotechnology, Jan 15, 2016
A simple kinetic model, with only three fitting parameters, for several enzyme productions in Pet... more A simple kinetic model, with only three fitting parameters, for several enzyme productions in Petri dishes by solid-state fermentation is proposed in this paper, which may be a valuable tool for simulation of this type of processes. Basically, the model is able to predict temporal fungal enzyme production by solid-state fermentation on complex substrates, maximum enzyme activity expected and time at which these maxima are reached. In this work, several fermentations in solid state were performed in Petri dishes, using four filamentous fungi grown on different agro-industrial residues, measuring xylanase, exo-polygalacturonase, cellulose and laccase activities over time. Regression coefficients after fitting experimental data to the proposed model turned out to be quite high in all cases. In fact, these results are very interesting considering, on the one hand, the simplicity of the model and, on the other hand, that enzyme activities correspond to different enzymes, produced by diff...
Advances in Food Biotechnology, 2015
Process Biochemistry, 2007
The feasibility of using grape pomace for the production of xylanase and exo-polygalacturonase by... more The feasibility of using grape pomace for the production of xylanase and exo-polygalacturonase by Aspergillus awamori in solid state fermentation has been evaluated. Solid state fermentation experiments indicated that the particle size did not influence the enzyme production. The addition of extra carbon sources and the initial moisture content of the grape pomace were found to have a marked influence on the enzymes yields. Xylanase and exo-PG activities were high at 65%(w/w) initial moisture content and glucose ...
Bioresource Technology, 2015
Evaluation of microwave-assisted pretreatment of lignocellulosic biomass immersed in alkaline gly... more Evaluation of microwave-assisted pretreatment of lignocellulosic biomass immersed in alkaline glycerol for fermentable sugars production, Bioresource Technology (2015),
Bioresource Technology, 2017
Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the pr... more Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the production of bio-products. Enzyme hydrolysis with the combined effect of mainly cellulases, xylanases and pectinases, turned out to be very efficient, obtaining almost double the concentration of sugars measured with the sole action of Celluclast® and βglucosidase, and increasing 5 times the hydrolysis rate. As the sole pretreatment, ESBC soaked in the hydrolysis buffer were autoclaved, avoiding the application of severe conventional biomass pretreatments. Moreover, a promising alternative for the complete utilization of glucose, xylose, arabinose, mannose and maltose contained in ESBC is proposed in this paper. It consists of sequential fermentation of sugars released in the hydrolysis step to produce bioethanol and lactic acid as main bio-products. Compared to separate fermentations, with this strategy glucose and hemicellulose derived sugars were completely consumed and the 44 % of pectin derived sugars.
Foods, 2020
In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as... more In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as a generic microbial culture medium, was carried out by using exhausted sugar beet pulp pellets (ESBPPs) as raw material. For this purpose, the hydrolysis was performed through the direct addition of the fermented ESBPPs obtained by fungal solid-state fermentation (SSF) as an enzyme source. By directly using this fermented solid, the stages for enzyme extraction and purification were avoided. The effects of temperature, fermented to fresh solid ratio, supplementation of fermented ESBPP with commercial cellulase, and the use of high-solid fed-batch enzymatic hydrolysis were studied to obtain the maximum reducing sugar (RS) concentration and productivity. The highest RS concentration and productivity, 127.3 g·L−1 and 24.3 g·L−1·h−1 respectively, were obtained at 50 °C and with an initial supplementation of 2.17 U of Celluclast® per gram of dried solid in fed-batch mode. This process was ca...
Foods, 2022
The agri-food sector produces large amounts of waste annually, most of which is lignocellulosic b... more The agri-food sector produces large amounts of waste annually, most of which is lignocellulosic biomass [...]
Bioethanol Production from Food Crops, 2019
Abstract The chemical composition of sugar beet roots makes this raw material an attractive feeds... more Abstract The chemical composition of sugar beet roots makes this raw material an attractive feedstock for ethanol fermentation. In the manufacture of sugar from this crop, various intermediates, by-products, and wastes are generated, which can be used for the production of energy and other value-added products, such as biofuels. In this chapter, the current processes for the conversion of the sugary juices (raw, thin, and thick juices), molasses and sugar beet pulp into bioethanol are described. In addition, the economics of sugar beet ethanol and the principal strategies proposed for increasing bioethanol yields are described.
Foods, 2021
Exhausted sugar beet pulp (ESBP), a by-product of the sugar industry, has been used as a substrat... more Exhausted sugar beet pulp (ESBP), a by-product of the sugar industry, has been used as a substrate to produce lactic acid (LA). Due to the fact that ESBP contains a high percentage of pectin and hemicellulose, different pretreatments were studied to solubilize them and to facilitate the access to cellulose in the subsequent enzymatic hydrolysis. Several pretreatments were studied, specifically biological, oxidant with alkaline hydrogen peroxide (AHP), and thermochemical with acid (0.25, 0.5, or 1% w/v of H2SO4). Pretreated ESBP was enzymatically hydrolysed and fermented with the strain Lactiplantibacillus plantarum for LA production. The hydrolysis was carried out with the commercial enzymes Celluclast®, pectinase, and xylanase, for 48 h. After that, the hydrolysate was supplemented with yeast extract and calcium carbonate before the bacteria inoculation. Results showed that all the pretreatments caused a modification of the fibre composition of ESBP. In most cases, the cellulose co...
Journal of the Science of Food and Agriculture, 2020
BACKGROUNDExhausted sugar beet pulp pellets (ESBPP) were used as raw material for lactic acid (LA... more BACKGROUNDExhausted sugar beet pulp pellets (ESBPP) were used as raw material for lactic acid (LA) fermentation. The enzymatic hydrolysis of ESBPP was performed with the solid obtained after the fungal solid‐state fermentation of ESBPP as a source of hydrolytic enzymes. Subsequently, a medium rich in glucose and arabinose was obtained, which was used to produce LA by fermentation. For LA production, two Lactobacillus strains were assayed and the effects of the supplementation of the hydrolysate with a nitrogen source and the mode of pH regulation of the fermentation were investigated. Moreover, a kinetic model for LA fermentation by Lactobacillus plantarum of ESBPP hydrolysates was developed.RESULTSL. plantarum produced a LA concentration 34% higher than that produced by L. casei. The highest LA concentration (30 g L−1) was obtained with L. plantarum when the hydrolysate was supplemented with 5 g L−1 yeast extract and the pH was controlled with CaCO3. The concentration of acetic aci...
Journal of the Science of Food and Agriculture, 2020
BACKGROUNDExhausted sugar beet pulp pellets (ESBPP), a sugar industry by‐product generated after ... more BACKGROUNDExhausted sugar beet pulp pellets (ESBPP), a sugar industry by‐product generated after sugar extraction in the sugar production process, have been used as a raw material for lactic acid (LA) production via hydrolysis and fermentation by Lactobacillus casei. To design a more cost‐effective process, simultaneous saccharification and fermentation (SSF) of ESBPP is proposed in the present study. The effects of pH control, nutrient supplementation and solid addition in fed‐batch SSF on lactic acid production were investigated.RESULTSThe highest LA concentration (26.88 g L–1) was reached in fed‐batch SSF at a solid/liquid loading of 0.2 g mL–1, with pH control (by adding 30 g L–1 CaCO3 to the medium) and nutrient supplementation (by adding 20 mL of MRS medium per 100 mL of buffer). Under these conditions, a maximum productivity of 0.63 g L−1 h−1 was achieved, which is 2.7 times higher than that attained in the control experiment (SSF inoculated at time 0 h). However, a slightly ...
Journal of the Science of Food and Agriculture, 2019
Background: Considering the large amounts of by-products derived from orange processing, generall... more Background: Considering the large amounts of by-products derived from orange processing, generally discarded, the aim of this article was to explore the feasibility of using orange peels for lactic acid production in solid state fermentation. Results: Different species of lactic acid bacteria were employed singly and in co-culture, in order to evaluate their ability to ferment orange peels and produce lactic acid. Among the single cultures tested, Lactobacillus casei 2246 was the most efficient strain, reaching the highest concentration of lactic acid (209.65 g/Kg) and yield (0.88 g/g). The employment of Lactobacillus plantarum 285 and Lactobacillus paracasei 4186 in co-culture, produced a comparable amount of lactic acid, showing better performance than the same strains in single cultures. Conclusion: Orange peels represent a suitable raw material for solid state fermentation employing lactic acid bacteria. Lactic acid was obtained consuming the most of sugars available leading to high yields. Despite all the strains tested showed the same growth ability, different peculiarities in lactic acid production depending on the species/strains were revealed suggesting the relevance on strains selection.
Waste Management & Research: The Journal for a Sustainable Circular Economy, 2018
Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, the... more Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydr...
Biofuels, Bioproducts and Biorefining, 2019
In this work, a mathematical model is proposed to predict the kinetic behaviour of the enzymatic ... more In this work, a mathematical model is proposed to predict the kinetic behaviour of the enzymatic conversion of various types of lignocellulosic biomass into fermentable sugars. Digestion of the cellulosic polymers is carried out using enzymatic hydrolysis under different conditions. Unlike other kinetic models, published previously for this process, this one considers the heterogeneous nature of the process by which a solid, in the form of small particles, is decomposed to monosaccharides by the action of a diverse set of enzymes in solution. The effect of the particle size on the hydrolysis rate has also been taken into consideration. To assess the model's goodness of fit to any general situation, the experimental data obtained in the hydrolysis of three different lignocellulosic residues have been analysed. Thus, the hydrolysis data of wheat straw, rice husks and exhausted sugar beet pellets have been compared with the theoretical values calculated by the model. The results obtained show that this model predicts the enzyme's hydrolysis of lignocellulosic substrates under different conditions very accurately and it could therefore be used efficiently in the optimization of the hydrolysis processes implemented in the bio-refinery industry.
Trends in Food Science & Technology, 2018
Background: Globally, significant amounts of agro-food residues are generated, most of which are ... more Background: Globally, significant amounts of agro-food residues are generated, most of which are burned as waste disposal. Given the abundance of this biomass, it can be used as raw material for the production of biofuels and valuable chemicals through biochemical conversion. Research in this field has gained importance given concerns regarding limitation of fossil resources, environmental problems and sustainability and preference towards natural, biodegradable and environmentally friendly products. Furthermore, the accessibility of alternative substrate sources for fermentative production of these high-value products has been one of the primary goals of industrial biotechnology research in the past two decades. Scope and approach: The present review is focused in the production of high value products through microbial fermentation of sugars from agro-food residues. Research in this field is interesting due to concerns regarding limitation of fossil resources. On the one hand, for the production of these bioproducts it is important the accessibility of alternative substrate and, on the other hand, the selection of the right microorganism to obtain the desired product. For this reason, the novelty of the present work is that details all different bioproducts which can be obtained through fermentation of sugars derived from agro-food residues, describing raw materials, pretreatments, mode of operation, conditions and microorganisms used, together with the production yields.
Applied microbiology and biotechnology, Jan 15, 2016
A simple kinetic model, with only three fitting parameters, for several enzyme productions in Pet... more A simple kinetic model, with only three fitting parameters, for several enzyme productions in Petri dishes by solid-state fermentation is proposed in this paper, which may be a valuable tool for simulation of this type of processes. Basically, the model is able to predict temporal fungal enzyme production by solid-state fermentation on complex substrates, maximum enzyme activity expected and time at which these maxima are reached. In this work, several fermentations in solid state were performed in Petri dishes, using four filamentous fungi grown on different agro-industrial residues, measuring xylanase, exo-polygalacturonase, cellulose and laccase activities over time. Regression coefficients after fitting experimental data to the proposed model turned out to be quite high in all cases. In fact, these results are very interesting considering, on the one hand, the simplicity of the model and, on the other hand, that enzyme activities correspond to different enzymes, produced by diff...
Advances in Food Biotechnology, 2015
Process Biochemistry, 2007
The feasibility of using grape pomace for the production of xylanase and exo-polygalacturonase by... more The feasibility of using grape pomace for the production of xylanase and exo-polygalacturonase by Aspergillus awamori in solid state fermentation has been evaluated. Solid state fermentation experiments indicated that the particle size did not influence the enzyme production. The addition of extra carbon sources and the initial moisture content of the grape pomace were found to have a marked influence on the enzymes yields. Xylanase and exo-PG activities were high at 65%(w/w) initial moisture content and glucose ...
Bioresource Technology, 2015
Evaluation of microwave-assisted pretreatment of lignocellulosic biomass immersed in alkaline gly... more Evaluation of microwave-assisted pretreatment of lignocellulosic biomass immersed in alkaline glycerol for fermentable sugars production, Bioresource Technology (2015),